Asian Journal of Plant Sciences - Current Issue

Mango Cultivars Uncovers Aroma Diversity and Chemotaxonomic Insights

10 June, 2026

Background and Objective: Mango (Mangifera indica L.) aroma is a key quality trait, yet the chemical diversity of volatile compounds across Vietnamese cultivars remains poorly characterized. This study aimed to profile volatile organic compounds (VOCs) responsible for aroma diversity and assess their potential for chemotaxonomic differentiation. Materials and Methods: Eight Vietnamese mango cultivars were analyzed using headspace gas chromatography-mass spectrometry (HS-GC/MS) to identify and quantify VOCs. Identified compounds were classified into major chemical groups and multivariate analyses were used to explore cultivar-specific profiles and potential chemotaxonomic markers. Results: A total of 95 VOCs was identified, grouped into alcohols, aldehydes and ketones, acids and esters, terpenes and terpenoids, hydrocarbons and miscellaneous compounds. The β-Terpinolene dominated six cultivars, notably Xoai Keo, Xoai Cat Chu and Xoai Cat Hoa Loc. Other cultivars exhibited distinctive markers: Xoai Thanh Ca was enriched in 3-carene and alpha-terpinolene, while Xoai Uc showed high D-limonene levels. Several terpenes were unique to individual cultivars, enabling chemical fingerprinting and differentiation. Conclusion: Volatile profiles, particularly monoterpenoid composition, effectively distinguish Vietnamese mango cultivars and serve as biochemical markers for breeding, authentication and conservation. These findings reveal untapped aroma diversity and support integrating aroma profiling into mango improvement programs.

Induction of Buds by Cold and Gibberellic Acid Pretreatment of Potato Tubers (Solanum tuberosum L.) for in vitro Plant Regeneration

10 June, 2026

Background and Objective: Potato (Solanum tuberosum L.) is a globally important food crop, yet its cultivation in tropical regions is limited by the absence of an efficient local seed production system. This study aimed to develop an integrated and reproducible protocol for in vitro regeneration of potato adapted to tropical environments. Materials and Methods: Tubers of two cultivars, Roseval and Charlotte, were subjected to four preconditioning treatments combining Gibberellic Acid (GA₃) and cold exposure to enhance bud induction before culture initiation. Excised buds were disinfected with Sodium Hypochlorite (NaOCl) or Calcium Hypochlorite [Ca(ClO)₂] at concentrations of 0.5, 1.0, and 2.0% active chlorine for varying exposure times. Regenerated plantlets were multiplied through successive subcultures on Murashige and Skoog (MS) medium supplemented with Morel and Wetmore vitamins and subsequently acclimatized on different substrates. Data were analyzed in R (v4.3.3) with arcsine-transformed percentages, ANOVA for cultivar, pretreatment, and disinfection effects, and Tukey’s HSD test for significant differences (p<0.05). Results: The combined GA₃+cold pretreatment significantly increased bud formation (20.3 buds per tuber in Roseval and 12.4 in Charlotte), confirming a synergistic effect on dormancy release and sprout development. Optimal disinfection was achieved using 1% active chlorine for 8 min, producing more than 86% healthy plantlets for both disinfectants. During acclimatization, sand and forest soil provided the highest survival rates (96.7% in Roseval and over 80% in Charlotte). Conclusion: This integrated approach harmonized pretreatment, disinfection, and acclimatization steps, enabling the efficient regeneration of vigorous and disease-free potato plantlets. The optimized protocol establishes a technical foundation for local seed potato production in West Africa, thereby contributing to regional food security and sustainable crop propagation.

Gelrite and Aerated Condition Enhanced Rooting and Growth of Pine Apple

10 June, 2026

Background and Objective: The in vitro development of Ananas sp. can be significantly influenced by the choice of gelling agent and culture conditions. Integrating agar and gelrite with aerated culture systems improves nutrient uptake and reduces physiological stress, enhancing plantlet quality and uniformity. The objective of this study was to optimize micropropagation of Ananas by evaluating the effects of gelling agents and aeration on growth and physiological responses. Materials and Methods: Shoots of 2 cm length were cultured on MS medium supplemented with 30 g/L sucrose and 1 mg/L IBA. Two gelling agents (agar and gelrite) were tested under aerated conditions (with or without filter paper) to assess their effects on rooting, growth and physiological stress parameters. Growth parameters, medium utilization, antioxidant enzyme activities (SOD and CAT), leaf yellowing and hormonal balance (IAA, CK, GA3 and melatonin) were recorded. All treatments were replicated thrice (15 vessels/treatment) and data were analyzed using Microsoft Excel 2016 and SPSS 20.0 with Duncan’s test at p<0.05. Results: Plantlets grown on gelrite under aerated conditions showed the highest growth, reaching 11.17 cm in height and 2170 mg fresh weight, with 90.16% medium utilization. Restricted aeration caused significant stress, including elevated SOD (189.88 U/g) and CAT (378.37 U/g) activities, 80% leaf yellowing in non-aerated agar treatments, reduced medium consumption (7.54% in non-aerated gelrite) and lower IAA/CK ratios. Aeration stabilized GA3, IAA and melatonin levels, reduced basal callus formation and produced uniform, healthy plantlets. Conclusion: Aerated culture with gelrite as a gelling agent enhances the efficiency of Ananas micropropagation. This method produces high-quality, uniform plantlets suitable for local cultivation and provides a valuable in vitro resource for future research.