https://scialert.net Journal of Biological Sciences en-us Science Alert Wed, 10 Jun 2026 18:11:57 +0200 Wed, 10 Jun 2026 18:14:14 +0200 RssPublisher 0.2.0 beta https://scialert.net/images/logo.gif https://scialert.net 41 233 Journal of Biological Sciences Assessment of Wound Healing Potentials of Methanol Extract of Anthocleista vogelii Leaves on Female Wistar Rats Background and Objective: Conventional drugs used in wound treatment are not only expensive but also have challenges of allergies and drug resistance. This necessitates the need to seek alternatives, especially in the area of medicinal plants which is still not fully explored. In this article wound healing potentials of methanol extract of Anthocleista vogelii leaves were studied. Materials and Methods: The 25 female Wistar rat (144-176 g) were divided into 5 groups of 5 rats each. Group 1 (normal control), group 2 and 3 were topically administered 0.1 g of Vaseline and 0.1 g Gentamicin, while groups 4 and 5 were administered 0.1 g Vaseline along with 0.1g extract (A. vogelii 1) and 0.2 g extract (A. vogelii 2), respectively for 14 days after inducing excision wound (2 cm) on the dorsal skin. An assay for biochemical and wound healing parameters was carried out using standard methods. Results: Wound healing studies revealed a significant increase in oestrogen level in group 4 and a significant decrease in iron level in group 5 when compared with group 1, 2 and 3. Hematological results revealed an increase in PCV, Hb, RBC, Platelets and RBC, WBC, neutrophil, eosinophil and monocyte on administration of Anthocleista vogelii leaves. Conclusion: The study thus revealed that Anthocleista vogelii leaves possess wound healing potential.]]> https://scialert.net/abstract/?doi=jbs.2025.1.12 10 June, 2026 Profilin and Vicilin Genes of Allergens in Fingerprinting of Minor Legumes Background and Objective: Coding regions of plants are a good source for designing the DNA markers to describe genetic variability in specific genomic parts. Here, the genetic variability of five selected minor legume species was analyzed by DNA markers for profilins and vicilins. Materials and Methods: Varieties of Phaseolus vulgaris L., Cicer arietinum L., Pisum sativum L., Lens culinaris Med. and Vicia faba L. were analyzed by profilin-based amplified polymorphism (PBAP) and vicilin based amplified polymorphism (VBAP) methods. The heterozygosity index, polymorphism information content, effective multiplex ratio, marker index, diversity index and discrimination power were calculated by iMEC. Dendrograms were constructed based on the obtained binary matrices using the Jaccard coefficient of genetic similarity. Polymorphism and marker techniques effectiveness were compared. Results: Profilin-based polymorphism was found to be more effective in generating polymorphic fingerprints in all the analyzed legume species. Vicilin-based polymorphism provides only a species-limited tool for genomic variability analysis in common beans and chickpeas. Conclusion: Profilin-based polymorphism is more effective in generating polymorphic fingerprints. Vicilin-based polymorphism provides a specie-limited tool for genomic variability analysis in legumes.]]> https://scialert.net/abstract/?doi=jbs.2025.13.22 10 June, 2026