https://scialert.net Research Journal of Microbiology en-us Science Alert Wed, 10 Jun 2026 18:11:57 +0200 Wed, 10 Jun 2026 18:14:14 +0200 RssPublisher 0.2.0 beta https://scialert.net/images/logo.gif https://scialert.net 41 233 Research Journal of Microbiology Quantitation of mecA and sea genes on Staphylococcus aureus using Quantitative PCR Assay Background and Objective: Methicillin Resistant Staphylococcus aureus (MRSA) is one of the major causes of nosocomial infections and are most profound in community in previously healthy individuals. To detect and quantify antibiotic resistant and virulence genes present in methicillin sensitive S. aureus (MSSA) strains from wounds and burns patients. Materials and Methods: About 200 clinical samples were obtained for S. aureus isolation, identified and characterized by using standard microbiological procedures. Methicillin resistance was determined by using β-lactamase assay and oxacillin disk (Oxoid) susceptibility test. Quantification of the S. aureus strains was performed using quantitative Polymerase Chain Reaction (qPCR) assay. Agarose gel electrophoresis was carried out on the qPCR products using 1.5% agarose gel with a standard DNA ladder (100 bp), visualized under UV transilluminator and the image taken using digital camera. Results: Almost 44 (22%) S. aureus were isolated and characterized with 36 (82%) strains producing β-lactamase and were resistant to oxacillin (MRSA) while, 8 (18%) strains do not produce β-lactamase and were sensitive to oxacillin (MSSA). The β-lactamase and non-β-lactamase isolates were resistant to other antibiotics. The quantification of PCR products indicated that sea genes (virulence enterotoxin factor) were detected from the antibiotic resistant staphylococci ranging from 0-13551.84 nmoles while, the quantification of mecA genes detected ranged from 0-2601.76 nmoles. The agarose gel electrophoresis of the PCR products of mecA and sea genes showed amplicon size of 657 bp for mecA and 526 bp for sea genes after amplification of the antibiotic resistant S. aureus strains. Conclusion: This study detected the presence of antibiotic resistant and virulence genes associated with MRSA in MSSA, which calls for urgent clinical and pharmaceutical attention.]]> https://scialert.net/abstract/?doi=jm.2021.1.7 10 June, 2026 Potential Antimicrobial Substances from the Characterized Bioactive Compounds Extracted from Secondary Metabolites of Aspergillus terreus Background and Objective: The menace of MDR bacteria and search for the potent antimicrobial substance remain a relevant research. Fungi are commonly recognized as microorganisms that play role in the production of secondary metabolites which are antimicrobials. Therefore, this study focused on the antimicrobial properties of the fungal secondary metabolites and their bioactive compounds. Materials and Methods: Soil samples were collected from the rhizosphere region of different farm gardens in Oyo town, Nigeria. Isolation of the fungi was carried out using Potato Dextrose agar, identified through the amplification of the ITS region of the ribosomal RNA operon and identified to be Aspergillus terreus. The isolates were screened for the production of secondary metabolites by batch culture using an incubator shaker at 27°C for 5 days. The metabolite was extracted and concentrated using a rotary evaporator and aliquots of the metabolites were stored at 4°C. Agar-well diffusion assay was employed to evaluate the antibacterial properties of the secondary metabolites of the fungus. The bioactive chemical compounds of the metabolite extracted from fungus were evaluated using the GC-MS technique. Results: The bacterial pathogens investigated in this work were multi-drug resistant bacteria with a minimum resistant rate of 61%. The MDR bacterial pathogens were all susceptible to the secondary metabolite of Aspergillus terreus in this study. Conclusion: The bioactive compounds evaluated in this work showed the occurrence of organic compounds in the metabolite therefore secondary metabolite of Aspergillus terreus holds the chance of discovering the novel and potent drug.]]> https://scialert.net/abstract/?doi=jm.2021.8.18 10 June, 2026