http://scialert.net Current Research in Tuberculosis en-us Science Alert Mon, 20 May 2013 18:11:57 +0200 Mon, 20 May 2013 18:14:14 +0200 RssPublisher 0.2.0 beta http://scialert.net/images/logo.gif http://scialert.net 41 233 Current Research in Tuberculosis 1819-3366http://feedburner.google.com Electrochemical Technique using Methylene Blue with Pencil Graphite Electrode for Optimum Detection of Mycobacterium tuberculosis DNA Mycobacterium tuberculosis (M. tuberculosis) using Pencil Graphite Electrode (PGE) and Methylene Blue (MB) as electroactive intercalators via non-covalent attachment was developed. Synthetic oligonucleotides of M. tuberculosis which consisted of M. Probe, M. Target, M. Non-complementary and M. Mutation were used for DNA hybridization detection on PGE. Differential Pulse Voltammetry (DPV) was performed using a PalmSens Electrochemical Portable Apparatus controlled by a Pocket PC. Various parameters affecting the response of the signals were explored and optimized including M. Probe concentration, immobilization time and hybridization time of immobilized M. Probe, concentration and accumulation time of MB, as well as the concentration of M. Target. The results obtained after measurement showed that the voltammetric signal of MB before hybridization was higher compared to after hybridization. This indicates that MB has high affinity towards guanine bases. Differences in the signals of MB for hybridization between M. Probes and Polymerase Chain Reaction (PCR) amplified products were observed. The Relative Standard Deviation (RSD) for TB PCR positive sample, TB PCR positive control, TB PCR negative sample, TB PCR negative control and TB PCR blank were 11.5, 2.9, 22.8, 13.8 and 20.3%, respectively which can be applied for the detection of M. tuberculosis.]]> http://scialert.net/abstract/?doi=crt.2012.1.12 20 May, 2013 Diagnostic Utility of Capilia TB Assay for Identification of Mycobacterium tuberculosis Complex Mycobacterium tuberculosis (M. tuberculosis) is important for tuberculosis control, so is the need for a rapid, simple and inexpensive identification test for mycobacterial culture isolates. The laboratories under Revised National Tuberculosis Control Programmes (RNTCP) in India are being scaled up by the introduction of rapid broth based culture systems. Laboratory mycobacterial culture isolates have to be further identified as M. tuberculosis or Non Tuberculous Mycobacteria (NTM) since accurate identification of M. tuberculosis complex is mandatory for appropriate diagnosis and treatment of tuberculosis. Biochemical tests are slow and sometimes require subcultures, as M. tuberculosis complex take weeks to grow. Capilia TB test is an immumno-chromatographic assay using monoclonal antibodies to detect MPB64 antigen/protein which is specific for M. tuberculosis complex (MTC). Therefore, the usefulness of Capilia TB test for culture confirmation of M. tuberculosis complex was evaluated in 75 mycobacteria positive clinical isolates by comparing it with conventional biochemical identification tests. The overall sensitivity and specificity was found to be 96.7 and 100%, respectively. The turn-around time for capilia ranged from 9-16 days as compared to biochemical identification which was 30-70 days. Capilia TB test is simple to perform and provides rapid confirmation of M. tuberculosis complex with minimal investment in terms of infrastructure, human labour and expertise. Laboratories using liquid culture may consider Capilia TB for rapid identification of M. tuberculosis complex.]]> http://scialert.net/abstract/?doi=crt.2012.13.18 20 May, 2013