http://scialert.net Asian Journal of Biotechnology en-us Science Alert Sun, 19 May 2013 18:11:57 +0200 Sun, 19 May 2013 18:14:14 +0200 RssPublisher 0.2.0 beta http://scialert.net/images/logo.gif http://scialert.net 41 233 Asian Journal of Biotechnology 1996-0700http://feedburner.google.com Protein Characterization of the Aqueous Soluble Phase of Acidified and Autolyzed Bolti Fish (Tilapia nilotica) Viscera Tilapia nilotica) viscera are either discarded leading to environmental pollution and wastage of bioresources. This biomass has however a potential to generate considerable revenue and can be turned into a commercially viable business. The objective of this study was to evaluate the proximate protein characterization of the aqueous soluble phase of acidified and autolyzed Bolti fish viscera. Solubilization of fish viscera at natural pH (6.2); slight acidified pH (5.2) as well as acidified pH (4.2 and 3.2) was determined. Autohydrolysis reactions were conducted on freshly thawed viscera utilizing gradient temperatures and terminated at various time points (0, 8, 18, 32 and 47 h) by heat inactivation of the endogenous enzymes. Viscera were then characterized with respect to total nitrogen content, soluble protein, ammonia content, amino acid compositions and protein molecular weight distribution. Interestingly, endogenous enzyme activities were sufficient for obtaining a solubilization yield protein of up to 30 g L^-1. SDS-PAGE profile showed that no characteristic band can be individualized for each hydrolysate and at the same time, greater variation was found only in the hydrolysate digested under initial pH 4.2 for 47 h. Amino acid analysis revealed that several amino acids were present in considerable amounts in the obtained hydrolysates, among them alanine, glutamic acid and proline. In addition, many hydrolysates contained useful quantities of the essential amino acids such as leucine, isoleucine, phenylalanine, histidine and valine and that may represent an interesting approach to upgrade these byproducts for food or feed purposes. In fact the results of our research would provide the incentive for commercial developments leading to large-scale and cost-effective production of fish peptone hydrolysates rich with essential amino acids from Bolti fish viscera.]]> http://scialert.net/abstract/?doi=ajbkr.2012.108.119 19 May, 2013 In vitro Micropropagation using Corm Bud Explants: An Endangered Medicinal Plant of Gloriosa superba L. in vitro micropropagation of Gloriosa superba by using corm bud explant. MS basal medium supplemented with different concentration and combination of 2,4-D (2,4-dichlorophenoxy acetic acid), IAA (Indole-3-acetic acid), BAP (6-benzyl aminopurine), GA₃ (Gibberellic acid), Zen (Zeatin), NAA (α-naphthaleneacetic acid), AC (Activated Charcoals) and CW (Coconut Water) were used. The 98.30±0.84% of yellowish Callus initiation was observed in MS media with (2, 4-D 1.0 mg L^-1, IAA 0.5 mg L^-1) after 4 week culture. This corm bud callus transferred to the shoots initiation medium. The maximum number (94.00±2.92) of multiple shoots was obtained on half strength of MS medium with (Kn (Kinetin) 1.0+BAP1.5+20% CW). The shoot lets transferred to the roots initiation medium. The 96.20±2.59% of multiple roots was obtained at the concentration of MS medium with BAP(8.0)+GA₃(1.0)+Zen(0.5)+NAA(1.0)+2 g L^-1 AC. In other case without addition of activated charcoal in the MS medium, only 92.20±1.92% of root initiation was occurred, 16% of root induction depends on the addition of activated charcoal present in the MS medium. The rooted plantlets were transferred into small plastic nursery tray which was containing vermi compost, sand and red soil in the ratio of 1:2:2 and kept in a mist house. After acclimatization in the mist house for 2-months, the regenerated plantlets were hardened in the greenhouse and successfully transferred into soil which shows 90% survival rate. This new protocol was standardized for easy mass propagation of such an endangered medicinal plant G. superba by using corm bud explants.]]> http://scialert.net/abstract/?doi=ajbkr.2012.120.128 19 May, 2013 Decolorization of Synthetic Dyes Using Bacteria Isolated from Textile Industry Effluent Bacillus subtilis (isolate B2, B7 and C1), Bacillus megaterium (isolate D3), Erysipelothrix (isolate C4) and Amphibacillus xylanus (isolate A1). These isolates were cultured with three different concentrations of seven different textile dyes viz. Cibacron red FN-R, novacron blue, terasil green, novacron navy, novacron orange and novacron yellow. Degradation ability of dye stuffs by the isolates (0.01, 0.05, 0.1 mg L^-1) was observed by dye decolorization assay. Almost all dyes except novacron red were decolorized up to 99% by bacterial isolates after 3 days of incubation.]]> http://scialert.net/abstract/?doi=ajbkr.2012.129.136 19 May, 2013 Comparative Study of Microbial Fuel Cell for Electricity Generation by Enriched Exoelectron Generating Bacteria from Environmental Samples -2 with graphite rod and 42.59 mW m^-2 with copper electrode and current density obtained was 108.57 mA m^-2 with graphite rod and 88.01 mA m^-2 with copper electrode all obtained with KMnO₄ solution.]]> http://scialert.net/abstract/?doi=ajbkr.2012.137.142 19 May, 2013 Regeneration of Plantlets from Leaf Derived Callus of Aerva lanata, Juss: A Medicinal Plant Aerva lanata of Amaranthaceae. Callus obtained by culturing young leaf discs on Murashige and Skoog^-1’s (MS) medium with 1.0 mg L^-1 2, 4.D (dichlorophenoxyacetic acid) was subcultured on MS medium containing 2.0 mg L^-1 6, Benzyl Amino Purine (BAP), produced 30-40 multiple shoots. Rooting was achieved on half or full strength MS medium with 0.5-0.1 mg L^-1 Naphthalene Acetic Acid (NAA) in 14 days. Regenerated plantlets were acclimatized and transferred to soil were showed normal morphological characteristics. Survival of transplants was 90-95% under green house condition.]]> http://scialert.net/abstract/?doi=ajbkr.2012.143.146 19 May, 2013