http://www.protocol-online.org/forums/index.php Mon, 06 Jul 2009 14:32:03 -0700 30 noBioforumhttp://feedburner.google.com http://feedproxy.google.com/~r/Bioforum/~3/bxT98y4sskg/index.php ÈçºÎ±ÜÃâPCRʱµÄ´íÎóÒý·¢£¿<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=gXblJYaD"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=ouXGEyeY"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=oNSqFPAg"><img src="http://feeds.feedburner.com/~f/Bioforum?i=oNSqFPAg" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=V1Oa3wEn"><img src="http://feeds.feedburner.com/~f/Bioforum?i=V1Oa3wEn" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=HzDapD3Z"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=Fro0TFPL"><img src="http://feeds.feedburner.com/~f/Bioforum?i=Fro0TFPL" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=dtSAyWwI"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=KaticsGU"><img src="http://feeds.feedburner.com/~f/Bioforum?i=KaticsGU" border="0"></img></a> </div> Wed, 30 Mar 2005 04:07:35 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5862 http://www.protocol-online.org/forums/index.php?showtopic=5862 http://feedproxy.google.com/~r/Bioforum/~3/1SPqSHW8Wok/index.php Ó¦ÓÃoligo-cappingµÄ·½·¨¹¹½¨È«³¤cDNAÎÄ¿âµÄʱºò£¬RTÍêÁË×öÁËÑéÖ¤£¬5¡®¶ËºÍ3¡¯¶ËÒýÎﶼûÓÐÎÊÌâ£<br />¬²âÐò½á¹ûÁ½¶Ë¶¼ÓÐsfi1λµã£¬µ«ÎÒÃÇ°ÑRT-PCRµÃµ½µÄcDNAºÍÔØÌå·Ö±ðÓÃsfi1øÇв¢Á¬½Óºó£¬×ª»¯µÃµ½µÄ¿Ë¡£¬ÓÃsfi1Çв»¿ª£¬ËµÃ÷¸ú±<br />¾Ã»Á¬ÉÏ£¬²»ÖªµÀÊÇʲôԭÒò£¿<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=L5R1y8so"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=CFu1CyG9"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=FvDUkinv"><img src="http://feeds.feedburner.com/~f/Bioforum?i=FvDUkinv" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=qZ6sDYYi"><img src="http://feeds.feedburner.com/~f/Bioforum?i=qZ6sDYYi" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=tq7zMRCb"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=lLo1HBUN"><img src="http://feeds.feedburner.com/~f/Bioforum?i=lLo1HBUN" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=qw39SiYD"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=E37m1FyX"><img src="http://feeds.feedburner.com/~f/Bioforum?i=E37m1FyX" border="0"></img></a> </div> Wed, 30 Mar 2005 04:04:15 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5861 http://www.protocol-online.org/forums/index.php?showtopic=5861 http://feedproxy.google.com/~r/Bioforum/~3/ayNVOMCTFFI/index.php ÎÒÃÇʵÑéÊÒ×î½üÓÃoligo-cappingµÄ·½·¨¹¹½¨È«³¤cDNAÎĿ⣬RTÍêÁËÖ®ºóÓÃEL1a·Ö±ð×öÁË5¡®ºÍ3¡¯¶ËµÄÑéÖ¤£¬Ö¤Ã÷ÎÒÃ<br />ǵÄÒýÎïûÓÐÎÊÌ⣬µ«ÎÒÃÇ×öÍêSFI1µÄøÇкÍÔØÌåÁ¬½Óºó£¬µÃµ½µÄ¿Ë¡²úÎïÓÃsfi1Çв»¿ª£¬Ë<br />µ<br />Ã÷¸ù±¾Ã»Á¬ÉÏ£¬¿É²âÐòÑéÖ¤ÒýÎïµÄÇеãÊÇÕýÈ·µÄ£¬ÔØÌåÒ²×ö¹ýÁ¬½ÓʵÑ飬ûÎÊÌâµÄ£¬Õæ²»Öª<br />µ<br />ÀÊÇÄĶù³öÁËÎÊÌ⣿<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=BucyAMIW"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=XrjXDtia"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=86Rgiafq"><img src="http://feeds.feedburner.com/~f/Bioforum?i=86Rgiafq" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=CvQcm3T2"><img src="http://feeds.feedburner.com/~f/Bioforum?i=CvQcm3T2" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=MDUVSGcx"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=y8dhVQNl"><img src="http://feeds.feedburner.com/~f/Bioforum?i=y8dhVQNl" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=V35SdupT"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=zgoCtemL"><img src="http://feeds.feedburner.com/~f/Bioforum?i=zgoCtemL" border="0"></img></a> </div> Tue, 29 Mar 2005 22:32:47 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5852 http://www.protocol-online.org/forums/index.php?showtopic=5852 http://feedproxy.google.com/~r/Bioforum/~3/kxMO1r9G77o/index.php ÎÒÃÇʵÑéÊÒ×î½üÓÃoligo-cappingµÄ·½·¨¹¹½¨È«³¤cDNAÎĿ⣬RTÍêÁËÖ®ºóÓÃEL1a·Ö±ð×öÁË5¡®ºÍ3¡¯¶ËµÄÑéÖ¤£¬Ö¤Ã÷ÎÒÃ<br />ǵÄÒýÎïûÓÐÎÊÌ⣬µ«ÎÒÃÇ×öÍêSFI1µÄøÇкÍÔØÌåÁ¬½Óºó£¬µÃµ½µÄ¿Ë¡²úÎïÓÃsfi1Çв»¿ª£¬Ë<br />µ<br />Ã÷¸ù±¾Ã»Á¬ÉÏ£¬¿É²âÐòÑéÖ¤ÒýÎïµÄÇеãÊÇÕýÈ·µÄ£¬ÔØÌåÒ²×ö¹ýÁ¬½ÓʵÑ飬ûÎÊÌâµÄ£¬Õæ²»Öª<br />µ<br />ÀÊÇÄĶù³öÁËÎÊÌ⣿<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=R9HSfH6P"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=hw7EjgkG"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=Wxk5C0di"><img src="http://feeds.feedburner.com/~f/Bioforum?i=Wxk5C0di" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=1Vf20mHX"><img src="http://feeds.feedburner.com/~f/Bioforum?i=1Vf20mHX" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=49jzc0y9"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=6I2it1JV"><img src="http://feeds.feedburner.com/~f/Bioforum?i=6I2it1JV" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=4bdPoCFr"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=TaIGIPce"><img src="http://feeds.feedburner.com/~f/Bioforum?i=TaIGIPce" border="0"></img></a> </div> Tue, 29 Mar 2005 22:31:46 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5851 http://www.protocol-online.org/forums/index.php?showtopic=5851 http://feedproxy.google.com/~r/Bioforum/~3/PGEM3qUZ930/index.php ʵÑéÐèÒªÓÃÏÔ΢ÇиîµÄ·½·¨´ÓʯÀ¯¹Ì¶¨µÄ´óÊó·Î°©×éÖ¯ÖÐÌáÈ¡DNA£¬µ«ÓÉÓÚ²¡Ôî½ÏС£¬ÏÔ΢¾<br />µÏ²ÅÄܼûµ½£¬¼ÓÉÏʯÀ¯×éÖ¯ÖÐDNAµÄÆÆ»µ£¬ÎÒµ£ÐÄÕâÑùÌáÈ¡µ½µÄDNA»á²»»áÌ«ÉÙ£¬ÎÞ·¨×ö¼×»<br />ù<br />»¯PCR£¿<br /> ²éÎÄÏ×·¢ÏÖ£¬ÓÐÒ»ÖÖ·½·¨½Ðnested, two stage MSP£¬¾ÍÊÇÏÈÉè¼ÆÒ»¶ÔÒýÎïÀ©ÔöÄ¿µÄ»ùÒò£¬È»ºóÔÙ½øÐÐMSP£¨Ó¦¸Ã¾ÍÊÇǶÌףУãң©£¬ÓÃÕâÖÖ<br />·½·¨ÊÇ·ñ¾Í¿ÉÒÔ½â¾öСÁ¿DNAµÄMSPÁËÄØ£¿<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=fzckOMFR"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=tbBkAeHs"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=3CUxTe0v"><img src="http://feeds.feedburner.com/~f/Bioforum?i=3CUxTe0v" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=WpHp16Kt"><img src="http://feeds.feedburner.com/~f/Bioforum?i=WpHp16Kt" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=y2oodaSg"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=ZwhNWgF7"><img src="http://feeds.feedburner.com/~f/Bioforum?i=ZwhNWgF7" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=3FIQ6L8B"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=6SRrc85H"><img src="http://feeds.feedburner.com/~f/Bioforum?i=6SRrc85H" border="0"></img></a> </div> Tue, 29 Mar 2005 01:08:00 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5827 http://www.protocol-online.org/forums/index.php?showtopic=5827 http://feedproxy.google.com/~r/Bioforum/~3/8X85GLGteEc/index.php ÎÒÏë×ö¹ØÓÚsd´óÊóµÄmsp,ÇëÎÊÓÐûÓÐÕâ·½ÃæµÄÏÖ³ÉÒýÎïÈçp16,mgmt,gstp,dpark,»òÕßÓÐûÓÐ<br />Êý¾Ý¿âʲôµÄ,ÎÒÒ»Ö±¹ØÐÄ×ÅÕâ¸öÂÛ̳,·Ç³£ÐÒÔËÄúÊǼ׻ù»¯·½ÃæµÄȨÍþ,ÖÕÓÚÒª¿ªÊ¼×öʵÑéÁ<br />Ë<br />,Ï£ÍûÄܵõ½hulÀÏʦµÄ°ïÖú,ллa<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=zr2r2Fzm"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=mdWc3vuP"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=uEjJkKgY"><img src="http://feeds.feedburner.com/~f/Bioforum?i=uEjJkKgY" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=TAUxDqP1"><img src="http://feeds.feedburner.com/~f/Bioforum?i=TAUxDqP1" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=8amiYAnU"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=PaRyGB81"><img src="http://feeds.feedburner.com/~f/Bioforum?i=PaRyGB81" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=ezLVUV6F"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=3TT12uLF"><img src="http://feeds.feedburner.com/~f/Bioforum?i=3TT12uLF" border="0"></img></a> </div> Mon, 28 Mar 2005 19:56:07 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5823 http://www.protocol-online.org/forums/index.php?showtopic=5823 http://feedproxy.google.com/~r/Bioforum/~3/y7kAoGYBwNE/index.php ÓиöÎÊÌâ²»Ã÷°×£¬ÎÒ¿´µ½²»Í¬µÄʵÑéÊÒÓõÄÑÇÁòËáÇâÄƵÄŨ¶È²»Í¬£¬ÎÒÊÇ°´HERMANÌá³öµÄ2.<br />6MµÄÖÕŨ¶È£¬ÓÐÈËÓÃ3.3M£¬»¹ÓÐÓøü¸ßŨ¶ÈµÄ£¬ÎÒÓõÄÊǹú²úµÄÊÔ¼Á£¬¶øHERMANÓõÄÊÇSIGM<br />A<br />µÄÊÔ¼Á£¬ÏëÇë½ÌÄú£º<br />1¡¢ÑÇÁòËáÇâÄÆ¡¢ÇâÑõ»¯Äƺʹ×Ëá淋ÄÖÕŨ¶ÈÓöà´óÁ¿ºÏÊÊ£¿<br />2¡¢´×Ëá淋Ä×÷ÓÃÊÇÖкͷ´Ó¦ÌåϵÖеļ»¹ÓÐ×÷Ϊ³Áµí·´Ó¦µÄÑΣ¬ÄÇô¸ÃÈçºÎÈ·¶¨ÆäÓÃÁ¿Ä<br />Ø£¿<br />3¡¢ÇâÑõ»¯ÄÆÔÙÐÞÊεĹý³ÌÖÐÓõ½Á½´Î£¬µÚÒ»´ÎÆð±äÐÔ×÷Ó㬻úÀíÊÇʲô£¨Ôõô±äÐÔ£©£¿µÚ¶<br />þ´ÎÊÇÍѻǻù×÷Ó㬷´Ó¦Ê½ÊÇʲô£¿<br />ÎÒÏëµ÷ÕûÎÒµÄÊÔÑ飬µ«ÓÖ²»Ã÷°×ÆäÖеÄϸ½Ú£¬Ï£Íû×ö¼×»ù»¯µÄÅóÓÑÄÜÓ»Ô¾²Î¼ÓÌÖÂÛ£¬²»ÖªµÀ<br />ÔÚÄÄÄÜÕÒµ½Õâ·½ÃæµÄ֪ʶ£¿Çë´ó¼Ò¸ø¸öÌáʾ£¡<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=XLSzura5"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=yXXZiMol"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=Mgzelt15"><img src="http://feeds.feedburner.com/~f/Bioforum?i=Mgzelt15" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=UtciIf8R"><img src="http://feeds.feedburner.com/~f/Bioforum?i=UtciIf8R" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=Wro3U9ew"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=imxjc9Ko"><img src="http://feeds.feedburner.com/~f/Bioforum?i=imxjc9Ko" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=fG9Q6ig7"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=QioO9TyW"><img src="http://feeds.feedburner.com/~f/Bioforum?i=QioO9TyW" border="0"></img></a> </div> Tue, 15 Mar 2005 03:29:45 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5595 http://www.protocol-online.org/forums/index.php?showtopic=5595 http://feedproxy.google.com/~r/Bioforum/~3/A31fO0iliI8/index.php ĿǰʵÑé¼±ÐèÕæºË±í´ïÔØÌåpIg£¬ÓÐÄÇλºÃÐÄÕßÌṩ»òÔںδ¦¹ºÂò£¬²»Ê¢¸Ð¼¤£¡<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=e9boQbv5"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=N0HpwVLU"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=GxyILS0m"><img src="http://feeds.feedburner.com/~f/Bioforum?i=GxyILS0m" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=p13XOHgW"><img src="http://feeds.feedburner.com/~f/Bioforum?i=p13XOHgW" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=aHtenq9N"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=9AUJOsAL"><img src="http://feeds.feedburner.com/~f/Bioforum?i=9AUJOsAL" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=BOmA3Fwe"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=gWBwYhvF"><img src="http://feeds.feedburner.com/~f/Bioforum?i=gWBwYhvF" border="0"></img></a> </div> Sat, 12 Mar 2005 19:53:05 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5561 http://www.protocol-online.org/forums/index.php?showtopic=5561 http://feedproxy.google.com/~r/Bioforum/~3/ETpYJoOyino/index.php <img src="http://www.protocol-online.org/forums/style_emoticons/default/sad.gif" style="vertical-align:middle" emoid=":(" border="0" alt="sad.gif" /> ³õ×öMSPµÄ¿ÉÁ¯µÄÎÒ,ΪʲôÎÒµÄpcr²úÎïÖ»Óжþ¾ÛÌå,¶øÇÒÊÇÖ»ÓÐÔÚ¼×»ù»¯ÒýÎï¼ÓÑÇÁòËáÇâÄÆ<br />´¦ÀíµÄÄ£°åµÄʱºòÓжþ¾ÛÌå,ÆäËûÇé¿öÁ¬Ìõ´ø¶¼Ã»ÓÐ<br />ÎÒ×öµÄÕâ¸ö»ùÒòûÓÐÎÄÏ×±¨µÀ¼×»ù»¯µÄ,ÎÒÏÖÔÚÑÏÖØ»³ÒÉÊÇÒòΪ´ó¼Ò¶¼Ã»×ö³öÀ´<img src="http://www.protocol-online.org/forums/style_emoticons/default/smile.gif" style="vertical-align:middle" emoid=":)" border="0" alt="smile.gif" /><br />Ôõô°ìѽ!¸÷λ°ï°ïÎÒ°É!!!<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=ibgIwOFj"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=pdnbjuRG"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=AGxARSSr"><img src="http://feeds.feedburner.com/~f/Bioforum?i=AGxARSSr" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=ntEslJho"><img src="http://feeds.feedburner.com/~f/Bioforum?i=ntEslJho" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=wHI5cgfJ"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=7ZO9gBXy"><img src="http://feeds.feedburner.com/~f/Bioforum?i=7ZO9gBXy" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=CmReoiuG"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=BPKjR4Qo"><img src="http://feeds.feedburner.com/~f/Bioforum?i=BPKjR4Qo" border="0"></img></a> </div> Wed, 09 Mar 2005 23:34:38 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5520 http://www.protocol-online.org/forums/index.php?showtopic=5520 http://feedproxy.google.com/~r/Bioforum/~3/2oOYlGNJDEw/index.php <img src="http://www.protocol-online.org/forums/style_emoticons/default/huh.gif" style="vertical-align:middle" emoid=":huh:" border="0" alt="huh.gif" /> ×·Ñ°ÁËn¾Ã£¬ÔÚDNA methylationµÄ°å¿é¿´µ½n¶àÈËÎÊÁËͬÑùµÄÎÊÌ⣬¾ÍÊÇÓÐʲô·½·¨¿ÉÒÔ²âoverall methylation£¬Ä¿Ç°ÎÒÒѾ­ÓÃ5£­m CµÄ¿¹Ìå×öÁËÃâÒßÓ«¹âºÍFACS£¬»¹ÏëÓÃHPLC À´quantifyһϣ¬µ«ÊÇÖ»ÖªµÀºÜ´ÖÂԵĹý³Ì£¬²»ÖªµÀÄÄλ´óϺÄܹ»²»ÁߴͽÌÏêϸµÄprotocol<br />£¬ÔÚÏ¸м¤²»¾¡¡£³ý´ËÒÔÍ⣬²»ÖªµÀÓÐʲô·½·¨¿ÉÒÔ¿´overall methyaltionµÄredistribution£¬Èç¹ûÖªµÀµÄͬ־Äܲ»ÄÜÇ뾡Á¿¸æËßdetailed protocol¡£Ð»Ð»ÁË <img src="http://www.protocol-online.org/forums/style_emoticons/default/rolleyes.gif" style="vertical-align:middle" emoid=":rolleyes:" border="0" alt="rolleyes.gif" /><div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=zSyWzuSi"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=gZM8Tquc"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=GtQrMGJk"><img src="http://feeds.feedburner.com/~f/Bioforum?i=GtQrMGJk" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=E48MSVB8"><img src="http://feeds.feedburner.com/~f/Bioforum?i=E48MSVB8" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=B9glwxew"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=ZMrfD9ep"><img src="http://feeds.feedburner.com/~f/Bioforum?i=ZMrfD9ep" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=Y9RdweWd"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=KusUpAjy"><img src="http://feeds.feedburner.com/~f/Bioforum?i=KusUpAjy" border="0"></img></a> </div> Tue, 08 Mar 2005 18:38:10 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5490 http://www.protocol-online.org/forums/index.php?showtopic=5490 http://feedproxy.google.com/~r/Bioforum/~3/hb2Typ3645I/index.php ¸÷λÐֵܽãÃÃÃÇ:<br /> ÇëÎÊÓÐË­ÖªµÀÔÚÄÄÀï¿ÉÒÔÂòµ½½ÍĸµÄÍ»±ä¾úÖꣿÊÇÌض¨ÒÑÖª»ùÒòλµãÍ»±äµÄ¾úÖê¡£ÖªµÀÍøÉÏ<br />ÁªÏµ·½Ê½Ò²¿É¡£<br /> ʵÑéËùÐ裬Çë´ó¼Ò¶à¶à°ï棬лл!<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=30FWLJ68"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=fa18dRbC"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=4lzdb1PR"><img src="http://feeds.feedburner.com/~f/Bioforum?i=4lzdb1PR" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=6NxNOMBi"><img src="http://feeds.feedburner.com/~f/Bioforum?i=6NxNOMBi" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=LRQJZRnK"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=51c6EEEo"><img src="http://feeds.feedburner.com/~f/Bioforum?i=51c6EEEo" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=hfytIson"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=4eEYEfFE"><img src="http://feeds.feedburner.com/~f/Bioforum?i=4eEYEfFE" border="0"></img></a> </div> Wed, 23 Feb 2005 23:52:55 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5312 http://www.protocol-online.org/forums/index.php?showtopic=5312 http://feedproxy.google.com/~r/Bioforum/~3/8s_GCFhb_eE/index.php ³õ´Îµ½´Ë£¬ÒÔºó»¹ÓкܶàÎÊÌâÏò´ó¼ÒÇë½Ì£¡<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=UTKHBIdV"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=UBtjR399"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=PC3IMuc8"><img src="http://feeds.feedburner.com/~f/Bioforum?i=PC3IMuc8" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=fA1e2xe1"><img src="http://feeds.feedburner.com/~f/Bioforum?i=fA1e2xe1" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=42R5vNYn"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=ePRqw0jF"><img src="http://feeds.feedburner.com/~f/Bioforum?i=ePRqw0jF" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=qNUGYk3v"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=kvvdWTb7"><img src="http://feeds.feedburner.com/~f/Bioforum?i=kvvdWTb7" border="0"></img></a> </div> Mon, 21 Feb 2005 04:10:06 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5272 http://www.protocol-online.org/forums/index.php?showtopic=5272 http://feedproxy.google.com/~r/Bioforum/~3/O2iA_LyhYYA/index.php [COLOR=blue]<br /> ´ó¼ÒºÃ£¬ÎÒÊÇÒ»ÃûÐÂÊÖ£¬¸Õ¸Õ½Ó´¥Õâ¸öÁìÓò£¬ÔÚ¿´ÊéµÄ¹ý³ÌÖÐÓкܶàµÄ²»Àí½â£¬ÏëÇó½Ì¸÷λ<br />Ç°±²Ò»¸ö·Ç³£¼òµ¥µÄÎÊÌ⣬ϣÍû´ó¼Ò²»ÒªÐ¦ÎÒŶ£¡ <img src="http://www.protocol-online.org/forums/style_emoticons/default/unsure.gif" style="vertical-align:middle" emoid=":unsure:" border="0" alt="unsure.gif" /> <br /> ÎÒÔÚ×¼±¸×öE¸ÆÕ³ËØ»ùÒòÆô¶¯×Ó¼×»ù»¯µÄÄÚÈÝ£¬ÎÒÏëÇëÎÊһϣ¬ÎÒÔõÑù²Å¿ÉÒԲ鵽Õâ¸ö»ùÒòÆ<br />ô¶¯×ÓµÄÐòÁС£<br /> ·Ç³£¸Ðл£¡<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=WslDdKwZ"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=pcJYDUjI"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=E0hSoLzn"><img src="http://feeds.feedburner.com/~f/Bioforum?i=E0hSoLzn" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=dj2hoDzO"><img src="http://feeds.feedburner.com/~f/Bioforum?i=dj2hoDzO" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=Ql7QxFPL"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=mnb0yw9P"><img src="http://feeds.feedburner.com/~f/Bioforum?i=mnb0yw9P" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=IVCQ30hm"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=g2TBrQml"><img src="http://feeds.feedburner.com/~f/Bioforum?i=g2TBrQml" border="0"></img></a> </div> Mon, 21 Feb 2005 01:40:58 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5270 http://www.protocol-online.org/forums/index.php?showtopic=5270 http://feedproxy.google.com/~r/Bioforum/~3/QOjR3aHbYTE/index.php ÐÂÄêºÃ!<br />ÎÒÊÇÒ»ÃûÐÂÊÖ,ÏëÏò¸÷λǰ±²Çë½ÌһЩÎÊÌâ,Çë¶à¶àÖ¸½Ì,лл!<br />ÎÒÕý×ö¿Ë¡»ùÒò·½Ãæ,²éÁËһЩ×ÊÁÏ,ÆäÖÐ×öcDNAºÏ³Éʱ,·´×ªÂ¼Ã¸ÓÐЩʹÓÃAMV,ÓÐЩʹÓÃMML<br />V.<br />ÇëÎÊʹÓÃÄÄÖÖµÄЧ¹û×îºÃ??<br />·Ç³£¸Ðл!<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=oVN98FgH"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=ViF95Anj"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=HIVApkJg"><img src="http://feeds.feedburner.com/~f/Bioforum?i=HIVApkJg" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=K4pL0wwb"><img src="http://feeds.feedburner.com/~f/Bioforum?i=K4pL0wwb" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=jUtWR3ig"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=MMhyWe5S"><img src="http://feeds.feedburner.com/~f/Bioforum?i=MMhyWe5S" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=NG2HT6Wr"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=9X0ZGfft"><img src="http://feeds.feedburner.com/~f/Bioforum?i=9X0ZGfft" border="0"></img></a> </div> Thu, 17 Feb 2005 00:38:32 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5225 http://www.protocol-online.org/forums/index.php?showtopic=5225 http://feedproxy.google.com/~r/Bioforum/~3/RgzEZ6vcoj8/index.php ´ó¼ÒºÃ£¬ÎÒÊÇÐÂÊÖ£¬ÂíÉϾÍÒª¿ªÊ¼×ö×Ô¼ºµÄʵÑéÁË£¬ÎÒÏëÔÚʵÑéµÄ¹ý³ÌÖÐÒ»¶¨»áÓöµ½ÕâÑù»ò<br />ÊÇÄÇÑùµÄÎÊÌ⣬ÒÔºóÃâ²»ÁËÂé·³´ó¼Ò£¬ÔÚÕâÀïÎÒÏÈлл´ó¼ÒÁË£¡ÒÔºó»¹Çë¶à¶à¹ØÕÕѽ <img src="http://www.protocol-online.org/forums/style_emoticons/default/smile.gif" style="vertical-align:middle" emoid=":)" border="0" alt="smile.gif" /><div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=RJRQMuBQ"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=jjJNl20M"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=OHoHULkI"><img src="http://feeds.feedburner.com/~f/Bioforum?i=OHoHULkI" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=qZ8lELv2"><img src="http://feeds.feedburner.com/~f/Bioforum?i=qZ8lELv2" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=DKtPUIuv"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=uwoqLSQn"><img src="http://feeds.feedburner.com/~f/Bioforum?i=uwoqLSQn" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=L8ns0Gl6"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=s9x45Qf4"><img src="http://feeds.feedburner.com/~f/Bioforum?i=s9x45Qf4" border="0"></img></a> </div> Tue, 01 Feb 2005 06:50:44 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5025 http://www.protocol-online.org/forums/index.php?showtopic=5025 http://feedproxy.google.com/~r/Bioforum/~3/_fa5n3Tdj2E/index.php ÎÒÕýÔÚÓÃÈ˵ĻùÒò×é×öMSP<br />1.±ØÐëøÇйýÒ¹Âð?<br />2.ÄÜ°ïÎÒ¿´Ò»ÏÂʵÑé²½ÖèÂð£¿<br />È¡»ùÒò×éDNA10 ul£¬Å¨¶ÈԼΪ100-200ng/ul£¬¼Ó40ulÃð¾ú´¿Ë®£¬»ìÔÈ¡£<br />¼ÓÐÂÏÊÅäÖÃ3MÇâÑõ»¯ÄÆÈÜÒº5 ul£¬42¡æ±£ÎÂ30·ÖÖÓ¡£<br />ÆÚ¼äÅäÖÆ40.5%ÑÇÁòËáÇâÄƺÍ10mMÇâõ«£¬ÆäÖÐÑÇÁòËáÇâÄÆÓÃ3MÇâÑõ»¯ÄƵ÷½ÚPHÖµÖÁ5¡£<br /> 10mMÇâõ«<br /> 40.5%ÑÇÁòËáÇâÄÆ 8.1g ÑÇÁòËáÇâÄÆ<br /> 17ml Ë®<br /> 10N ÇâÑõ»¯ÄÆ µ÷pHµ½5.0<br /> ¼ÓË®ÖÁ20 ml<br />È¡³öÑùÆ·£¬¼ÓÈë10mMÇâõ«30ul£¨ÈÜÒºÑÕɫת»Æ£©£¬<br />¼ÓÈë40.5%ÑÇÁòËáÇâÄÆ510 ul£¬Îý²­Ö½°ü¹üÑùÆ·¡£<br />ÇáÈá»ìÔÈ£¬55¡æ±£ÎÂ16h¡£<br />ÓÃDNA clean-upÊÔ¼ÁºÐ´¿»¯DNA£¬ÊÕ¼¯50ulÏ´ÍÑÒº¡£<br />¼Ó5.5ulŨ¶ÈΪ3NµÄÇâÑõ»¯ÄÆ£¬»ìÔÈ£¬37¡æ·ÅÖÃ15·ÖÖÓ¡£<br /> È»ºóÔÙ¼Ó8MÒÒËá泥¨pH7.0)ÈÜÒº35ul£¬»ìÔÈ¡£<br />¼ÓÔ¤ÀäÎÞË®ÒÒ´¼300ul£¬µßµ¹»ìÔÈ£¬ÖÃ-20¡æ±ùÏä¹ýÒ¹¡£<br /> È¡³öÑù±¾£¬13200rpmÀëÐÄ20min£¬ÆúÈ¥ÉÏÇ壬¼Ó70%ÒÒ´¼100ul£¬13200rpmÀëÐÄ2min£¬ÆúÈ¥ÉÏÇ壬ÁÀ¸ÉDNA£¬¼ÓÈëÃð¾ú´¿Ë®30ul¡£<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=WFttnoB2"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=PYNaxNXg"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=EDbx2LIQ"><img src="http://feeds.feedburner.com/~f/Bioforum?i=EDbx2LIQ" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=owp6iowK"><img src="http://feeds.feedburner.com/~f/Bioforum?i=owp6iowK" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=QDzLlF0Y"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=X0G2NVQw"><img src="http://feeds.feedburner.com/~f/Bioforum?i=X0G2NVQw" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=eB2AUfpd"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=7LJrcRae"><img src="http://feeds.feedburner.com/~f/Bioforum?i=7LJrcRae" border="0"></img></a> </div> Tue, 01 Feb 2005 04:31:16 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5024 http://www.protocol-online.org/forums/index.php?showtopic=5024 http://feedproxy.google.com/~r/Bioforum/~3/zCr0IqNqaVI/index.php ÎÒÕýÔÚ×öÓйØĤ(¼´ÄáÁúĤÉÏÉÏǧ¸öPCRÔö·ùƬ¶Î),µ«µÃµ½µÄÓ°ÏñÎļþ¿´µ½,Ò»²¿·Öspots²»Çå<br />³þ,¼¸´ÎʵÑé¾ùÈç´Ë.ºÃÏñÊÇÒòΪĤºÍ°ü¹üÔÚËüÖ®ÉϵÄÄáÁú²¼Ö®¼äĦ²ÁËùÖÂ.<br />Ï£ÍûÓд˾­ÑéµÄÈËÄܸæËßÎÒÔõô´¦Àí.<br />лл.<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=sSTAfCP8"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=7p70Wf0a"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=B6KoWArp"><img src="http://feeds.feedburner.com/~f/Bioforum?i=B6KoWArp" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=ajHAcZoS"><img src="http://feeds.feedburner.com/~f/Bioforum?i=ajHAcZoS" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=E0shL9Ew"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=RBJcl04z"><img src="http://feeds.feedburner.com/~f/Bioforum?i=RBJcl04z" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=kDa6RFnZ"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=jSL3yfFJ"><img src="http://feeds.feedburner.com/~f/Bioforum?i=jSL3yfFJ" border="0"></img></a> </div> Tue, 01 Feb 2005 02:04:04 -0800 http://www.protocol-online.org/forums/index.php?showtopic=5017 http://www.protocol-online.org/forums/index.php?showtopic=5017 http://feedproxy.google.com/~r/Bioforum/~3/8MGH2tFhWVg/index.php ÎÒµÄMSP»ù±¾³É¹¦£¬Ö¤Ã÷ÒýÎïÊÇ¿ÉÒԵģ¬ÓÐÒ»µã¾­Ñé¾ÍÊÇÈÈÆô¶¯PCR·Ç³£ÖØÒª£¬ÓÈÆäÔڷǼ׻ù<br />»¯ÌØÒìÐÔÒýÎïÀ©ÔöÌåϵÖУ¬·ÇÈÈÆô¶¯²»¿É£¡<br />ÏÂÒ»²½´òËã½øÐж¨Á¿MSP£¬ÏÖÔÚÕý¿àÓÚÑ¡Ôñ̽ÕëÓм¸¸öÎÊÌâÇë½Ì£º<br />1. ÒýÎïÉè¼ÆÓë̽ÕëºÏ³É£º¾­¹ýMETHPRIMERÉè¼ÆµÄÁ½¶ÔÒýÎïµÄTmÖµ·Ö±ðΪ£º¼×»ù»¯ÌØÒìÐÔ69.54£<br />¬69.57£»·Ç¼×»ù»¯ÌØÒìÐÔ59.88£¬65.94£»ÒýÎïºÏ³Éµ¥ÉÏÓëÈí¼þ¸ø³öµÄTmÖµÏà²î½Ï´ó£¬ÒªµÍ5¶<br />È<br />×óÓÒ£¬¶øÔÚʵ¼ÊMSP¹ý³ÌÖзǼ׻ù»¯ÌØÒìÐÔÒýÎïµÄÍË»ðζÈÖ»ÓÐ52¶È£¬¶¨Á¿PCRµÄ̽ÕëÒªÇóÒý<br />Î<br />ïÓнϸߵÄÍË»ðζȣ¬¿ÉÊDz»Í¬µÄ̽ÕëºÏ³É¹«Ë¾¿Ú¾¶ÍêÈ«²»Í¬£¬ÓеÄ˵²»Äܺϳɣ¬ÓеÄ˵ÍêÈ<br />«<br />¿ÉÒԺϳɣ¬ÎÒÓÐЩµ£ÐÄ¡£<br />2. ̽ÕëÐòÁз½Ã棺ÎÒ¿´µ½µÄ²Î¿¼ÎÄÏ×Éϼ׻ù»¯ÌØÒìÐÔÓë·Ç¼×»ù»¯ÌØÒìÐÔÌåϵµÄ̽ÕëÐòÁÐÊDz»Í¬<br />µÄ£¬¶øÇÒ»¹°üÀ¨ÁË1£­2¸öCpG¼î»ù¶Ô¡£ÎÒ×Ô¼ºÈÏΪÒýÎïÉè¼ÆÒѾ­Äܹ»Çø·Ö¼×»ù»¯Óë·Ç¼×»ù»¯Ð<br />ò<br />ÁУ¬Ì½ÕëÊÇ·ñ¿É²»±ØÔÙ°üÀ¨CpG¼î»ù¶Ô£¿ÕâÑùÁ½¸öÌåϵ¿É¹²ÓÃÒ»¸ö̽Õ룬һ·½Ãæ½ÚʡһЩ£¬Á<br />í<br />Ò»·½Ãæ̽ÕëÉè¼Æ·½Ãæ¾ÍûÓÐÄÇô¾ÖÏÞ£ºÎÒµÄÁ½¶ÔÒýÎïÖ®¼äµÄÐòÁп¿½üÉÏÓÎÒýÎïµÄµØ·½Ö»ÓÐÒ»<br />¸<br />öCpG¼î»ù¶Ô£¬Ì½ÕëÉè¼Æ·¶Î§±È½Ï¾ÖÏÞ¡£<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=OGelbhqP"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=gp8j2SQo"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=RNZE33CS"><img src="http://feeds.feedburner.com/~f/Bioforum?i=RNZE33CS" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=KPMaY0v1"><img src="http://feeds.feedburner.com/~f/Bioforum?i=KPMaY0v1" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=ALj5oCDq"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=GmSHLz6L"><img src="http://feeds.feedburner.com/~f/Bioforum?i=GmSHLz6L" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=QAJ9VEwr"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=QfxXmfIm"><img src="http://feeds.feedburner.com/~f/Bioforum?i=QfxXmfIm" border="0"></img></a> </div> Wed, 12 Jan 2005 17:30:50 -0800 http://www.protocol-online.org/forums/index.php?showtopic=4798 http://www.protocol-online.org/forums/index.php?showtopic=4798 http://feedproxy.google.com/~r/Bioforum/~3/d_NbARhnw_E/index.php Hi, everyone <br />I want to study a protein functions. It is found that this protein locates in <br />both nucleus and cytoplasm but mainly aggregate in nucleus. So what kind of <br />functions I can guess this protein has and what kind of experiments I can <br />try? <br /> <br />Thank you<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=glkKaEbJ"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=ngFG1mIB"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=EgKnctPq"><img src="http://feeds.feedburner.com/~f/Bioforum?i=EgKnctPq" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=PdKxsCRG"><img src="http://feeds.feedburner.com/~f/Bioforum?i=PdKxsCRG" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=T99BSkL0"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=2m7FI5mI"><img src="http://feeds.feedburner.com/~f/Bioforum?i=2m7FI5mI" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=EYo3xrVV"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=crAo67D6"><img src="http://feeds.feedburner.com/~f/Bioforum?i=crAo67D6" border="0"></img></a> </div> Sun, 09 Jan 2005 20:01:54 -0800 http://www.protocol-online.org/forums/index.php?showtopic=4755 http://www.protocol-online.org/forums/index.php?showtopic=4755 http://feedproxy.google.com/~r/Bioforum/~3/0LdgT1lCgFg/index.php ÎÒÊÇÐÂÀ´µÄ£¬Ïȱ¨¸öµ½°¡£¡£¡£¡<br /><br />ÕâÁ½Ìì¿´ÎÄÏ×£¬·¢ÏÖÓÐЩÎÄÏ×ÔÚÉè¼ÆÒýÎïµÄʱºòÌرðÓÃÀ¨ºÅÀ¨³öÒ»¸ö×î5'¶ËµÄÖ»ÓÐCGµÄ³¤¶Ì²»Ò»µÄƬ¶Ï£¬ËµÊÇʲôCG Clamp£¬²»ÖªÕâÊǸöʲô¶«¶«°¡£¿<br /><br /><br />Õâ¸öÂÛ̳ÊÇÎÒÒ»Ö±ÔÚÕҵģ¬Ï£ÍûÄÜЭÖú°æÖ÷°ÑËü¸ãµÄ¸üÍú£¡£¡£¡<div class="feedflare"> <a href="http://feeds.feedburner.com/~f/Bioforum?a=T0PEEdWF"><img src="http://feeds.feedburner.com/~f/Bioforum?d=41" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=9Zo27h58"><img src="http://feeds.feedburner.com/~f/Bioforum?d=52" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=Q8PqL9N1"><img src="http://feeds.feedburner.com/~f/Bioforum?i=Q8PqL9N1" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=zznS3xi3"><img src="http://feeds.feedburner.com/~f/Bioforum?i=zznS3xi3" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=4YCNPtj2"><img src="http://feeds.feedburner.com/~f/Bioforum?d=54" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=rSCYBepT"><img src="http://feeds.feedburner.com/~f/Bioforum?i=rSCYBepT" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=IfqIlrYJ"><img src="http://feeds.feedburner.com/~f/Bioforum?d=129" border="0"></img></a> <a href="http://feeds.feedburner.com/~f/Bioforum?a=SkzroLUw"><img src="http://feeds.feedburner.com/~f/Bioforum?i=SkzroLUw" border="0"></img></a> </div> Sat, 08 Jan 2005 05:28:32 -0800 http://www.protocol-online.org/forums/index.php?showtopic=4744 http://www.protocol-online.org/forums/index.php?showtopic=4744 en-usnonadult