tag:blogger.com,1999:blog-7493153214778385929Wed, 04 Jan 2012 07:07:18 +0000We wish we not only do science but live it, just as how hot fudge lives with ice cream. Science is fun, as fun as sitting under the sun, with ice cream melting all over our faces.http://gohotfudge.blogspot.com/noreply@blogger.com (wl)Blogger362125tag:blogger.com,1999:blog-7493153214778385929.post-7608590266762477188Tue, 03 Jan 2012 05:42:00 +00002012-01-02T23:44:30.027-06:00 Nature 473, 337–342 Received 16 November 2010 Accepted 01 April 2011 Published online 18 May 2011 The Selbath group in Germany used NIH313T mammalian cell line as a model to quantify cellular mRNA and protein expression levels, and the half life of proteins.  They found mRNA level is the best indicator to predict protein abundance in an individual cell, but not the half life of a given http://feedproxy.google.com/~r/HotFudge/~3/QZf6IefW7iY/protein-ranking-inside-cell.htmlnoreply@blogger.com (wl)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/QZf6IefW7iY" height="1" width="1"/>http://gohotfudge.blogspot.com/2012/01/protein-ranking-inside-cell.htmltag:blogger.com,1999:blog-7493153214778385929.post-433556762352357123Tue, 03 Jan 2012 05:00:00 +00002012-01-02T23:00:09.658-06:00 Nature Medicine (2011) doi:10.1038/nm.2600 Received 20 December 2010 Accepted 26 May 2011 Published online 25 December 2011 Another paper which improves microscope imaging technology by optimizing clearing procedure of fixed tissues to allow the samples to be fully penetrable for optical imaging.  With the newly-developed clearing procedure, they may make the spinal cord transparent http://feedproxy.google.com/~r/HotFudge/~3/QNAi0guXhlg/as-far-as-light-can-go.htmlnoreply@blogger.com (wl)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/QNAi0guXhlg" height="1" width="1"/>http://gohotfudge.blogspot.com/2012/01/as-far-as-light-can-go.htmltag:blogger.com,1999:blog-7493153214778385929.post-7424742115916694335Tue, 03 Jan 2012 04:44:00 +00002012-01-02T22:45:06.365-06:00 Nature Medicine (2011) doi:10.1038/nm.2559 Received 12 January 2011 Accepted 14 October 2011 Published online 18 December 2011 The story was a back-to-back two papers in the latest issue of Nature Medicine.  Both groups found a deacetylase, Sirt 1, may protect mice from neurodegeneration of Huntington's disease.  The Krainc lab generated a conditional mouse line with brain specific http://feedproxy.google.com/~r/HotFudge/~3/3AO3m-yTaN8/nature-medicine-2011-doi10.htmlnoreply@blogger.com (wl)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/3AO3m-yTaN8" height="1" width="1"/>http://gohotfudge.blogspot.com/2012/01/nature-medicine-2011-doi10.htmltag:blogger.com,1999:blog-7493153214778385929.post-5810598813210031374Mon, 02 Jan 2012 05:31:00 +00002012-01-01T23:31:21.891-06:00 Published Online December 15 2011 Science DOI: 10.1126/science.1213495 I first noticed the above paper because 1) it was right below the "mycobacteria grow asymmetrically" paper Jen mentioned in previous post. 2) it's from Steve Reiner's lab. Then, there're only TWO figures with reasonable size of panels. What a beautiful story teller.  I guess I always have a weakness for a clean story, http://feedproxy.google.com/~r/HotFudge/~3/_JgQ2GXPE3Y/b-cell-divide-asymmetrically.htmlnoreply@blogger.com (wl)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/_JgQ2GXPE3Y" height="1" width="1"/>http://gohotfudge.blogspot.com/2012/01/b-cell-divide-asymmetrically.htmltag:blogger.com,1999:blog-7493153214778385929.post-4040186133072732378Mon, 02 Jan 2012 04:11:00 +00002012-01-01T22:11:45.296-06:00 Published Online December 1 2011 Science 23 December 2011: Vol. 334 no. 6063 pp. 1727-1731 DOI: 10.1126/science.1206936 The blood-brain barrier tightly controls what molecules and cells may entry into the brain. Astrocytes are known to critically contribute, in a contact dependent way, to maintain BBB integrity and immune quiescence by releasing soluble factors. Prat lab now suggested SHH http://feedproxy.google.com/~r/HotFudge/~3/JsrNPnVqppc/secure-brain.htmlnoreply@blogger.com (wl)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/JsrNPnVqppc" height="1" width="1"/>http://gohotfudge.blogspot.com/2012/01/secure-brain.htmltag:blogger.com,1999:blog-7493153214778385929.post-8821361011166472003Tue, 20 Dec 2011 22:57:00 +00002011-12-20T17:10:25.044-06:00The TB field noticed mycobacteria are more heterogeneitic than other bacteria (say E. coli or Bacillus, not too much further) for a long time. We assume that can contribute to the drug resistance and dormancy but we haven't been able to figure out the mechanism. The Fortune lab has been interested in disecting the mechanisms. Here they combine microfluidics and fluoromicroscopy and found out http://feedproxy.google.com/~r/HotFudge/~3/tJJ4ODjxaCI/mycobacteria-grow-asymmetrically.htmlnoreply@blogger.com (Jun)3<img src="http://feeds.feedburner.com/~r/HotFudge/~4/tJJ4ODjxaCI" height="1" width="1"/>http://gohotfudge.blogspot.com/2011/12/mycobacteria-grow-asymmetrically.htmltag:blogger.com,1999:blog-7493153214778385929.post-1638915825784069070Tue, 29 Nov 2011 17:44:00 +00002011-11-29T11:44:05.853-06:00 David M. Dosa, M.D., M.P.H. N Engl J Med 2007; 357:328-329July 26, 2007http://feedproxy.google.com/~r/HotFudge/~3/FKsbmYBDddM/oscar-cat-nejm.htmlnoreply@blogger.com (wl)1<img src="http://feeds.feedburner.com/~r/HotFudge/~4/FKsbmYBDddM" height="1" width="1"/>http://gohotfudge.blogspot.com/2011/11/oscar-cat-nejm.htmltag:blogger.com,1999:blog-7493153214778385929.post-7872401063590270556Sun, 27 Nov 2011 05:43:00 +00002011-11-26T23:53:22.841-06:00 Published online before print November 21, 2011, doi: 10.1073/pnas.1108487108 PNAS November 21, 2011 As title (yep.. the paper is sooo straightforward and can be easily summarized in one sentence)Human ES cell derived neurons can modulate the excitability of pre-existing mouse neurons in vitro and in vivo, and also form the new functional synapse with mouse neurons. Hmm... "Wow" http://feedproxy.google.com/~r/HotFudge/~3/lIQ22FyjKLs/cross-species-cooperation.htmlnoreply@blogger.com (wl)1<img src="http://feeds.feedburner.com/~r/HotFudge/~4/lIQ22FyjKLs" height="1" width="1"/>http://gohotfudge.blogspot.com/2011/11/cross-species-cooperation.htmltag:blogger.com,1999:blog-7493153214778385929.post-1718529856180480313Sun, 27 Nov 2011 05:04:00 +00002011-11-26T23:31:30.495-06:00 Nature Biotechnology (2011) doi:10.1038/nbt.2038 Received 02 May 2011 Accepted 12 October 2011 Published online 13 November 2011 Flow cytometry + microfluidic technologies on a single cell basis first got my attention.  Yet the data turned out to be surprisingly cool-- The group analyzed gene expression pattern of normal human colon epithelium, and compared to the profile of a primary http://feedproxy.google.com/~r/HotFudge/~3/iXwccMaWxJI/world-in-cell.htmlnoreply@blogger.com (wl)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/iXwccMaWxJI" height="1" width="1"/>http://gohotfudge.blogspot.com/2011/11/world-in-cell.htmltag:blogger.com,1999:blog-7493153214778385929.post-3802195346708458390Sun, 27 Nov 2011 03:05:00 +00002011-11-26T22:09:35.850-06:00 Sci. Signal., 15 November 2011 Vol. 4, Issue 199, p. ra77 [DOI: 10.1126/scisignal.2002246] I like this paper a lot.  So while thinking to seriously re-start the blog, the said paper is the first one that had pupped to my head that I would like to share.  Positive selection may be merely a process obeying thermodynamic law; a process that to build a common language between T cell and http://feedproxy.google.com/~r/HotFudge/~3/8rsiorNy_pk/fated-to-survive.htmlnoreply@blogger.com (wl)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/8rsiorNy_pk" height="1" width="1"/>http://gohotfudge.blogspot.com/2011/11/fated-to-survive.htmltag:blogger.com,1999:blog-7493153214778385929.post-5216667223041458473Sun, 27 Nov 2011 00:13:00 +00002011-11-26T18:21:20.610-06:00 Science 25 November 2011:  Vol. 334 no. 6059 p. 1046  DOI: 10.1126/science.334.6059.1046-a The week of science was dedicated to a mini series of 6 open questions in terms of "cell."  The first two questions was related to immunology, and thus got my attention.   The series was well written, and definitely a fun to read.   Enjoy. It feels like a whole century since the last http://feedproxy.google.com/~r/HotFudge/~3/ERnweGJ5ATg/reading-is-fundamental.htmlnoreply@blogger.com (wl)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/ERnweGJ5ATg" height="1" width="1"/>http://gohotfudge.blogspot.com/2011/11/reading-is-fundamental.htmltag:blogger.com,1999:blog-7493153214778385929.post-8383756741863498154Sat, 24 Sep 2011 12:30:00 +00002011-09-24T07:54:24.079-05:00還需要進一步確認，但要是確定的話，將打破愛因斯坦狹義相對論裡光速是最快的速度！http://www.nature.com/news/2011/110922/full/news.2011.554.htmlhttp://feedproxy.google.com/~r/HotFudge/~3/L1VZwZuPQGo/blog-post.htmlnoreply@blogger.com (Jun)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/L1VZwZuPQGo" height="1" width="1"/>http://gohotfudge.blogspot.com/2011/09/blog-post.htmltag:blogger.com,1999:blog-7493153214778385929.post-6759530053090953163Thu, 01 Sep 2011 17:49:00 +00002011-11-26T18:24:33.004-06:00 原文聯結 日本團隊發現一種新試劑scale 能讓生物組織樣本變透明（靠減少light scattering） 便於顯微鏡觀察 而且不影響螢光訊號 這真是太強了http://feedproxy.google.com/~r/HotFudge/~3/NVlH7IEjyQE/scale-up.htmlnoreply@blogger.com (wl)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/NVlH7IEjyQE" height="1" width="1"/>http://gohotfudge.blogspot.com/2011/09/scale-up.htmltag:blogger.com,1999:blog-7493153214778385929.post-691989133143994782Thu, 25 Aug 2011 03:04:00 +00002011-08-24T22:30:40.618-05:00A few important papers just got published recently! One is the real target of pyrazinamide, a first line drug to combat tuberculosis. http://www.sciencemag.org/content/early/2011/08/10/science.1208813 The corresponding author identified the bacterial enzyme (pyrazinamidase) that coverts the pro-drug into the real toxic (pyrozinoic acid) inside of the bacteria in 1996. And only after this many http://feedproxy.google.com/~r/HotFudge/~3/EdOPvfQ5_Ao/good-month-to-field-of-infectious.htmlnoreply@blogger.com (Jun)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/EdOPvfQ5_Ao" height="1" width="1"/>http://gohotfudge.blogspot.com/2011/08/good-month-to-field-of-infectious.htmltag:blogger.com,1999:blog-7493153214778385929.post-7922747868029546031Fri, 15 Jul 2011 15:16:00 +00002011-11-27T00:19:50.076-06:00 Published Online 14 July 2011 Science DOI: 10.1126/science.1207745 Psychology studies always intrigues me, as somehow it shone the beauty of science that how to nail down a question with careful experiment design.  Sparrow and Wegner group investigated whether the  the advent of the Internet would impact how human beings put effort to memorize facts.  You might not be too http://feedproxy.google.com/~r/HotFudge/~3/krQkF6aRTgY/google-effect.htmlnoreply@blogger.com (wl)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/krQkF6aRTgY" height="1" width="1"/>http://gohotfudge.blogspot.com/2011/07/google-effect.htmltag:blogger.com,1999:blog-7493153214778385929.post-664449733296606622Tue, 12 Jul 2011 19:24:00 +00002011-07-12T14:24:20.121-05:00http://www.nature.com/news/2011/110223/full/470437a.html We had a speaker come in yesterday who spoke about the Decline Effect, and I'll just quote the first paragraph of his commentary in Nature here: "Many scientifically discovered effects published in the literature seem to diminish with time. Dubbed the decline effect, this puzzling anomaly was first discovered in the 1930s in research intohttp://feedproxy.google.com/~r/HotFudge/~3/1k5u14OOubI/decline-effect.htmlnoreply@blogger.com (TCA)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/1k5u14OOubI" height="1" width="1"/>http://gohotfudge.blogspot.com/2011/07/decline-effect.htmltag:blogger.com,1999:blog-7493153214778385929.post-8345238230169390521Sat, 19 Mar 2011 17:54:00 +00002011-03-19T13:13:19.219-05:00Professor Sigal Ben-Yehuda 本來是 Rich Losick 的博士後，有超漂亮的 publication record.一開始只是她的題目跟一個同事有 overlap 所以注意到她的文章。這篇則是一個之前沒有被發現過的 cell-cell communication method. 細菌會形成一些 nanotube 傳遞 intracellular molecule. 他們也有一些圖去證明這跟傳統的 pilus 是不一樣的 (粗很多)。(點圖到 Cell 網頁有精彩影片)(我其實是一直在想一開始他們怎麼會想到把不一樣的細菌混在一起.... 或許是想觀察 biofilm？Rich & Roberto 滿多 Bacillus biofilm formation 的研究會用到帶不一樣的螢光蛋白的細菌... 不過當然得跟作者聊才會知道內情啦)http://feedproxy.google.com/~r/HotFudge/~3/YXfG2IuAGCM/novel-way-of-intercellular.htmlnoreply@blogger.com (Jun)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/YXfG2IuAGCM" height="1" width="1"/>http://gohotfudge.blogspot.com/2011/03/novel-way-of-intercellular.htmltag:blogger.com,1999:blog-7493153214778385929.post-4676107037398748619Sat, 19 Mar 2011 17:51:00 +00002011-03-19T12:53:56.675-05:00http://www.bbc.co.uk/news/world-asia-pacific-12726591http://feedproxy.google.com/~r/HotFudge/~3/_Mfc2jRbsck/clear-explanation-of-nuclear-disaster.htmlnoreply@blogger.com (Jun)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/_Mfc2jRbsck" height="1" width="1"/>http://gohotfudge.blogspot.com/2011/03/clear-explanation-of-nuclear-disaster.htmltag:blogger.com,1999:blog-7493153214778385929.post-3796020014597166976Wed, 23 Feb 2011 15:52:00 +00002011-02-23T09:53:29.444-06:00 Nature 470, 399–403 (17 February 2011) doi:10.1038/nature09728 A new gene named 24 was found to be critical in rhythms regulation.  I posted this as an effort to apologize that I told a good friend that I don't really have stomach for gene regulation. http://feedproxy.google.com/~r/HotFudge/~3/QchMkY-wZgg/24.htmlnoreply@blogger.com (wl)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/QchMkY-wZgg" height="1" width="1"/>http://gohotfudge.blogspot.com/2011/02/24.htmltag:blogger.com,1999:blog-7493153214778385929.post-5413956135205497288Wed, 16 Feb 2011 20:02:00 +00002011-02-16T14:02:01.333-06:00 Nature 469, 419–423 (20 January 2011) doi:10.1038/nature09674 Except for the well known Toll-like receptors and et cetera, innate immunity also uses various antimicrobial peptides, such as defensins, to fight the bacteria in the gut, and protect the human.  Among the class of defensin, β-defensin 1 is probably the most prominent (quotes the paper), yet what remains puzzled is the detectable http://feedproxy.google.com/~r/HotFudge/~3/yhkSOn89qNE/redox-rocks.htmlnoreply@blogger.com (wl)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/yhkSOn89qNE" height="1" width="1"/>http://gohotfudge.blogspot.com/2011/02/redox-rocks.htmltag:blogger.com,1999:blog-7493153214778385929.post-743668341993156535Fri, 12 Nov 2010 17:40:00 +00002010-11-12T11:40:24.894-06:00Anyone remembered the Reactome Array on Science?  Here's the retraction. http://feedproxy.google.com/~r/HotFudge/~3/Iat0SZd5H5A/retractome-array.htmlnoreply@blogger.com (wl)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/Iat0SZd5H5A" height="1" width="1"/>http://gohotfudge.blogspot.com/2010/11/retractome-array.htmltag:blogger.com,1999:blog-7493153214778385929.post-3207652305976205980Tue, 09 Nov 2010 08:29:00 +00002010-11-09T02:55:20.704-06:00Scientists of McMaster University in Canada reported that transduction of skin fibroblasts with Oct4 make fibroblasts transform into heamatopoietic progenitor cells. Only Oct4, but not other Yamanaka factors (Nanog or Sox2), could do this. The Oct4 transduced skin fibroblasts, no matter from adult or neonatal, can further differentiate into granulocytes, megakaryocytes, erythrocytes and monocyteshttp://feedproxy.google.com/~r/HotFudge/~3/LzPZC4uEwqI/make-blood-from-your-skin.htmlnoreply@blogger.com (wenwei)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/LzPZC4uEwqI" height="1" width="1"/>http://gohotfudge.blogspot.com/2010/11/make-blood-from-your-skin.htmltag:blogger.com,1999:blog-7493153214778385929.post-1518741626646204996Mon, 11 Oct 2010 07:38:00 +00002010-10-11T02:38:26.762-05:00 The above list was written by the award winner Douglas L Coleman (A historical perspective on leptin) The Lasker Basic Medical Research Award-- Douglas Coleman and Jeffrey Friedman(for the roles of leptin in developing obesity) The Lasker~DeBakey Clinical Medical Research Award-- Napoleone Ferrara(for defining VEGF as an angiogenic factor and treating macular degeneration by anti-VEGF http://feedproxy.google.com/~r/HotFudge/~3/Bamv4zTV69Q/2010-lasker-award.htmlnoreply@blogger.com (wl)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/Bamv4zTV69Q" height="1" width="1"/>http://gohotfudge.blogspot.com/2010/10/2010-lasker-award.htmltag:blogger.com,1999:blog-7493153214778385929.post-8120949325483456479Sun, 03 Oct 2010 21:03:00 +00002010-10-03T16:03:45.969-05:00Okay, after being lazy for such a long time, let's start with some strikingly dark side of science. linkage: Published online 29 September 2010 Nature 467, 516-518 (2010) | doi:10.1038/467516ahttp://feedproxy.google.com/~r/HotFudge/~3/2Xq99IzInFQ/rr.htmlnoreply@blogger.com (wl)2<img src="http://feeds.feedburner.com/~r/HotFudge/~4/2Xq99IzInFQ" height="1" width="1"/>http://gohotfudge.blogspot.com/2010/10/rr.htmltag:blogger.com,1999:blog-7493153214778385929.post-8623576458356816951Wed, 22 Sep 2010 23:47:00 +00002010-09-22T19:00:22.743-05:00In this paper, authors used an interesting way to make monoclonal antibody. Without going through all of the chimera-fusion and screening process, they sequence identified the most abundant repertoire of the variable region gene sequence. Then they directly synthesized the DNA sequence to make the constructs and use bacteria or mammalian cells to make the monoclonal antibody. Antibodies made viahttp://feedproxy.google.com/~r/HotFudge/~3/PR-2R1SmcIA/alternative-way-to-make-monoclonal.htmlnoreply@blogger.com (Jun)0<img src="http://feeds.feedburner.com/~r/HotFudge/~4/PR-2R1SmcIA" height="1" width="1"/>http://gohotfudge.blogspot.com/2010/09/alternative-way-to-make-monoclonal.html