LifeTein, the peptide and protein synthesis service company

Rush Peptide Synthesis in Action

2019-11-13T17:43:00.002-05:00

Introducing LifeTein's faster microwave peptide synthesis technology!

LifeTein's new platform is designed for maximized speed and efficiency. Unparalleled peptide quality, greater flexibility, and improved reliability make LifeTein the vendor of choice for all your peptide synthesis needs.

Remarkably Fast:

Rush synthesis service in 3-5 Days
Difficult peptides such as beta-Amyloid in hours rather than days
4-minute cycle time

LifeTein provides the fastest turnaround time and most reliable quality in the industry. Peptides are made in New Jersey, USA. Projects move from conception to bench in only 3–5 days so you can deal with your research deadlines.

Accelerate Your Research: Move your peptides to the top of the queue at every step in the peptide synthesis process. LifeTein peptides are synthesized and shipped 40% faster than the industry standard. Actual shipping times are based on the individual peptide and protein characteristics.

Made in the USA 🇺🇸: Rest assured that your rush peptide orders are made in New Jersey, 100% accurate and compliant with all intellectual property laws.

LifeTein’s Synthetic Scorpion Toxin Peptides Helped Scientists Unravel Chronic Pain Mechanisms

2019-11-13T11:01:00.002-05:00

LifeTein’s synthetic Wasabi Receptor Toxin, Wasabi Receptor Toxin Mutants, Biotinylated Wasabi Receptor Toxin, and AlexaFluor-488 conjugated Wasabi Receptor Toxin and Mutants helped scientists unravel chronic pain mechanisms.

Researchers at the University of California, San Francisco (UCSF) have identified a scorpion toxin that targets the “wasabi receptor”. The wasabi receptor is an ion channel protein that is responsible for the sinus-clearing or eye-stinging pain experienced when eating wasabi or chopping onions.

It was found that the scorpion toxin, a peptide as the wasabi receptor toxin, or WaTx, activates the wasabi receptor TRPA1 and triggers this pain response to irritants. The WaTx peptide is a novel cell-penetrating peptide and can directly pass through the plasma membrane, without needing to traverse through channel proteins.

The WaTx peptide could be used to study chronic pain and inflammation and may lead to the development of novel non-opioid pain therapies. WaTx produces pain and pain hypersensitivity, but not neurogenic inflammation.

https://www.lifetein.com/blog/lifeteins-synthetic-scorpion-toxin-peptides-helped-scientists-unravel-chronic-pain-mechanisms/

Reference: Lin King, J. V., Emrick, J. J., Kelly, M. J. S., Herzig, V., King, G. F., Medzihradszky, K. F., & Julius, D. (2019). A Cell-Penetrating Scorpion Toxin Enables Mode-Specific Modulation of TRPA1 and Pain. Cell. doi:10.1016/j.cell.2019.07.014

A click chemistry was reported about the formation of azides from primary amines

2019-11-13T10:54:00.003-05:00

A click chemistry was reported about the formation of azides from primary amines. This powerful tool enables the reaction of just one equivalent of a simple diazotizing species, and fluorosulfuryl azide (FSO2N3), for the preparation of over 1,200 azides on 96-well plates in a safe and practical manner. This method greatly expands the number of accessible azides and 1,2,3-triazoles because the primary amine is one of the most abundant functional groups in small compounds, proteins and antibodies.

The method opens the door for numerous applications in drug screening and discovery. The cell penetration peptides can be easily introduced to conjugate with any azide containing drugs, compounds, antibodies, or proteins.

The cell penetration peptides (CPPs) are capable of delivering biologically active cargo to the cell interior. The desired therapeutic cargo could be attached to a CPP using the copper free click chemistry and then delivered to an intracellular target, thereby overcoming the entry restrictions set by the plasma membrane.

How to form the fibrillary structure using beta-amyloid peptides?

2019-11-13T10:53:00.003-05:00

Aβ-(1–42) was dissolved to 1 mM in 100% hexafluoroisopropanol, hexafluoroisopropanol was removed under vacuum, and the peptide was stored at −20 °C. For the aggregation protocols, the peptide was first resuspended in dry Me2SO (DMSO) to 5 mM. For oligomeric conditions, F-12 (without phenol red) culture media was added to bring the peptide to a final concentration of 100 μM, and the peptide was incubated at 4 °C for 24 h. For fibrillar conditions, 10 mM HCl was added to bring the peptide to a final concentration of 100 μM, and the peptide was incubated for 24 h at 37 °C. ADDLS, amyloid derived diffusible ligands.

Aducanumab is a human monoclonal antibody that has been studied for the treatment of Alzheimer’s disease.

The Nobel Prize in Physiology or Medicine 2019 was awarded jointly to William G. Kaelin Jr, Sir Peter J. Ratcliffe and Gregg L. Semenza

2019-11-13T10:51:00.002-05:00

https://www.lifetein.com/blog/the-nobel-prize-in-physiology-or-medicine-2019-was-awarded-jointly-to-william-g-kaelin-jr-sir-peter-j-ratcliffe-and-gregg-l-semenza/

The Nobel Prize in Physiology or Medicine 2019 was awarded jointly to William G. Kaelin Jr, Sir Peter J. Ratcliffe and Gregg L. Semenza. One of the key peptides was made by LifeTein: HIF1a-P564: DLDLEMLAPYIPMDDDFQLR.

Winter Promotion: Keep warm with a Land's End fleece

2014-12-17T13:14:00.001-05:00

Winter Promotion: Keep warm with a Land's End fleece ($59 Value, US Customer Only)

From Dec. 8, 2014 to Dec. 31, 2014 get a free fleece ($59 Value) for any purchase of $500 or more*.

See offer details. *View terms and conditions. *Limit one offer per customer. No combining offers. Not applicable to prior purchases. Limited to stock on hand. Void where prohibited.

http://www.lifetein.com/free-gift-promotions.html

25% Off peptide synthesis for new customers plus free shipping

2014-12-17T13:12:00.001-05:00

LifeTein, the peptide synthesis service company: LifeTein Peptide Cited in Cell

2014-11-11T12:52:00.001-05:00

LifeTein, the peptide synthesis service company: LifeTein Peptide Cited in Cell: Peptide library is increasingly used to define antibody epitopes and substrate specificities of protein kinases. For epitope mapping, overla...

LifeTein Peptide Cited in Cell

2014-11-11T12:43:00.001-05:00

Peptide library is increasingly used to define antibody epitopes and substrate specificities of protein kinases. For epitope mapping, overlapping peptides are made to span the antigenic protein sequence. The antigenic determinant recognized by a monoclonal antibody can then be screened and defined. The alanine scanning method can also be used to assess that residue’s contribution to antibody binding and to determine which substitutions affect antibody recognition (mutational analysis). Unrelated synthetic peptides can be used to evaluate the antibody cross-reactivity.

Overlapping peptides from LifeTein were used to map the region of Fragment 3 by epitope mapping of anti-Fzd2 antibody. This anti-Fzd2 antibody was found to reduce tumor growth. Wnt signaling plays a critical role in colorectal cancer. Researchers found that Wnt receptor Frizzled2 (Fzd2) and its ligands Wnt5a/b are elevated in metastatic liver, lung, colon, and breast cancer cell lines. Their high level expression correlates with markers of epithelial-mesenchymal transition (EMT). By epitope mapping using synthetic peptides from LifeTein, the researchers mapped the epitope to a specific region. The antibody to Fzd2 was found to reduce cell migration and invasion. Targeting this pathway may provide a cure for patients with tumors expressing high amount of Fzd2 and Wnt5a/b.

We have developed an antibody to Fzd2 that reduces cell migration and invasion and inhibits tumor growth and metastasis in xenografts. We propose that targeting this pathway could provide benefit for patients with tumors expressing high levels of Fzd2 and Wnt5a/b.

Cell, Volume 159, Issue 4, 6 November 2014, Pages 844–856, DOI: 10.1016/j.cell.2014.10.032 A Noncanonical Frizzled2 Pathway Regulates Epithelial-Mesenchymal Transition and Metastasis

Synthetic peptide from LifeTein used for drug discovery

2014-11-01T16:01:00.002-04:00

Synthetic TAT peptides from LifeTein were used for fluorescence resonance energy transfer (FRET) assay. This arginine-rich TAT peptide contains two fluorophores with FAM at the N terminus and TAMRA at the C terminus.

Three alanines were used as a spacer to avoid potential interference on the binding affinity against TAR. The sequence used in this study is: (5‑FAM)‑ AAARKKRRQRRRAAA-K(TAMRA)‑C.

The Tat peptide causes two fluorophores to form a dimer, quenching the fluorescence of both fluorophores. When the Tat peptide is bound to the bulge of TAR, the peptide transitioned to the extended conformation.

In the study, researchers have identified 11 small molecule inhibitors of TAR‑Tat interaction from high throughput screening (HTS) and virtual screening (VS). One of the compounds showed promising anti‑HIV activity. The hits from HTS and VS suggested that the HTS and VS are complimentary to each other. HTS and VS will boost the efficiency of the drug discovery.

http://scholar.google.com/scholar_url?hl=en&q=http://deepblue.lib.umich.edu/bitstream/handle/2027.42/108771/janghlee_1.pdf%3Fsequence%3D1%26isAllowed%3Dy&sa=X&scisig=AAGBfm20J8EkWT4LD8Rg3mQnJHE7mEZr_w&oi=scholaralrt

Amidated neuropeptides by LifeTein for receptor binding study

2014-10-18T15:32:00.001-04:00

Neuropeptides plays critical roles in every aspect of life. The honey bee short neuropeptide F (sNPF) was synthesized by LifeTein. These neuropeptides were amidated at the C terminus to mimic the native protein structure. The amidated neuropeptide ligands mutated to a different amino acid could not activate the receptor. The amidated Y amino acid is critical for receptor activation and decrease in intracellular cAMP production.

The result is supported by detection of a signature peptide fragment by MALDI-TOF MS and by functional cAMP assays.

(Reference: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109590)

Synthetic phospho-peptide for castor oil seed studies

2014-10-18T14:56:00.000-04:00

Synthetic phospho-peptide from LifeTein were used for the castor oil seed studies. The purified phosphopeptides at Serine from LifeTein confirmed that the phosphorylation can protect sucrose synthase isozyme RcSUS1from proteolysis. The phosphorylation process is modulated by photosynthesis in leaves. Using polypeptides is an important tool to develop effective biotechnological strategies. reference: http://www.jbc.org/content/early/2014/10/13/jbc.M114.585554.short

A breakthrough for drug delivery into tumours using LifeTein cell penetrating peptides

2014-10-07T14:55:00.001-04:00

Biotin labeled peptides were synthesized by LifeTein and conjugated with nanoparticles. A 6-amino-hexanoic linker was added between biotin and peptide to form a flexible space. It was found that a peptide designed for tumour-homing with a carboxy-terminal, basic sequence motif can act as cell penetration peptide to enter tumour tissue. This is a breakthrough for drug delivery into tumours. In addition, this neuropilins mediated endocytosis is distinct from known endocytic pathways. The nutrient regulation of the pathway is a new finding to use of the C-terminal, basic sequence motif pathway in drug delivery.

http://www.lifetein.com/Quality-Peptides-For-Publications.html

How to dissolve peptides with organic solvents

2014-10-02T10:05:00.002-04:00

Dissolving a hydrophobic peptide could be tricky. LifeTein's website offers free information for researchers on how to dissolve peptides in polar organic solvents such as DMSO or ethanol. The website on how to handle and dissolve peptides was cited in Extremophiles. Lifetein's guideline is helpful for researchers to understand the basic procedure on how to dissolve peptides using the best solvent without sacrifice the protein activity at non-aqueous environments.

The reference was published on Extremophiles: DOI: 10.1007/s00792-014-0683-4

Effect of organic solvents on the structure and activity of moderately halophilic Bacillus sp. EMB9 protease

R Sinha, SK Khare - Extremophiles

Read more on how to handle and dissolve peptides: http://www.lifetein.com/handling_and_storage_of_synthetic_peptides.html

Custom antibodies were used to study SKPO-1

2014-09-25T17:00:00.001-04:00

Custom peptide antibodies were provided by LifeTein to study SKPO-1. The SKPO-1 peroxidase domain peptide was synthesized by LifeTein. The polyclonal antibodies were raised in rabbits and then affinity purified to accomplish high specifications and concentrations. The antibodies were successfully used to localize the SKPO-1 protein in hypodermis. It was found that the peroxidase SKPO-1 contributes to the host immune response during infection. http://www.lifetein.com/antibody_services.html

Synthetic peptides used for identification of SOX-CAL binding site

2014-09-25T11:53:00.001-04:00

Synthetic peptide from LifeTein were used to identify the SOX-CAL binding site. Long peptides including helix 1 of the HMG domain were synthesized by LifeTein. The peptides were used to examine the interaction of CaM with the CaM-binding domain of SOX9 (SOX-CAL). The researcher found two binding sites: the well-know high-affinity CaM binding site, and a lower-affinity binding site.

Please click here for the article: http://onlinelibrary.wiley.com/doi/10.1002/cbic.201402414/abstract

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