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Anybody knows how to decode this?

PubMed

• Retrieve PubMed citations in HTML or Text format using PubMed IDs (PMID)

• Search PubMed with terms

• PubMed Link to related records

• PubMed Feature Pages

NLM currently leases PubMed journal citations, at no charge.

Display Formats

Display Formats for Sample Entrez Databases

Note: Scripts/programs that import XML should use E-Utilities.

To remove mod_security from your site, edit .htaccess file:
add these lines:

<IfModule mod_security.c>
###########################################
# disable POST processing to not break multiple image upload

    SecFilterEngine Off
    SecFilterScanPOST Off
</IfModule>

<IfModule mod_deflate.c>

If this does not work, remove mod_security from your httpd.conf by commenting out the following lines:

AddModule mod_security.c
LoadModule security_module modules/mod_security.so
Include “/usr/local/apache/conf/modsec.conf”

 Or move mod_security.conf from conf.d directory to somewhere else.

$ grep -H -r "Ski resort" /home/innovita


Search "Ski resort" in a current directory and sub-directory

$ grep -H -r "Ski resort" .

app/locale/en_US/template/email.

1, Upload a new logo to

images/logo_email.gif

2, Change any file with *new.html

alt=”Magento” –> alt=”InnoVita”

3, Change any file with confirmation.html

 Demo Store –>  InnoVita

4, Change email and phone number

 dummyemail@magentocommerce.com –>me@my.com
magento@varien.com –>me@my.com
(555) 555-0123

5, To make sure you have changed everything
 

cd  /home/xxx/webapps/app/locale/en_US/template/email
cat *.html |grep Demo
cat *.html |grep 555
cat *.html |grep magento

cd sales
cat *.html |grep Demo
cat *.html |grep 555
cat *.html |grep magento

openssl genrsa -des3 -out innovita.key 2048
openssl req -new -key innovita.key -out innovita.csr

2, Upload csr file to GOoDaddy website to generate certificate files. Download the zip file to your server and unzip it.

3, use this command to remove password so apache will start automatically without asking for password.

openssl rsa -in innovita.key -out innovita.key.insecure
innovita.key innovita.key_w_password
cp innovita.key.insecure innovita.key

4, Configure ssl.conf file, add these lines:

SSLCertificateFile /home/innovita/ssl/innovitainc.com.crt
SSLCertificateKeyFile /home/innovita/ssl/innovita.key
SSLCertificateChainFile /home/innovita/ssl/gd_bundle.crt

5, Restart Apache

Apachectl restart

Example: Transfect Hela cells using GeneExpresso and PolyExpress in vitro DNA transfection reagent:

Of course you will see more clearly under fluorescence microscope:

Transfection in the presence of serum and antibiotics

Transfection in the absence of serum and antibiotics

* provide product protection only and must not be used when working with any form of biohazard or chemical hazard
* any potentially infectious aerosol that is created will lead to exposure of the operator and the environment
* horizontal-flow clean-air bench used for cell cultures can expose the researcher to aerosols of allergenic or infectious materials.
* vertical-flow clean-air bench also blows air out into the room

Biological Safety Cabinets

* provide personnel and environmental protection and commonly product protection
* infectious agents must be used in a biological safety cabinet NOT a laminar flow hood

Class I Biological Safety Cabinet

* a ventilated cabinet which provides personnel and environmental protection only
* air flow is directed away from the researcher, but is not HEPA filtered, therefore there is no product protection
* similar to a fume hood with a HEPA filter on the exhaust system to protect against the release of biohazards
* inward air flow ranges from 75-125 linear feet per minute (lfpm)
* can be used with radioisotopes and some toxic chemicals

Class II Biological Safety Cabinet

* provides personnel, product and environmental protection
* there are supply air and exhaust air HEPA filters
* two general types: IIA cabinets have a minimum inward air flow of 75 lfpm and recirculates 70% of the air; IIB cabinets have a minimum inward air flow of 100 lfpm and exhaust either 70% (type B1) or 100% (type B2)
* most of the biological safety cabinets at UVic are Class II

Class III Biological Safety Cabinet

* these cabinets provide personnel, product and environmental protection
* they are hermetically sealed and all procedures are conducted through arm-length rubber gloves
* used in high level (Level 4) containment labs
* there are two HEPA filters on the exhaust system

1, Write a script in Perl or Java to search pubmed by date and keyword.

2, If there is “next” link on the result page, let script to click on “next” link until there is no more “next” link.

3, extract abstract link from search result pages

4, pause 15~ 25  seconds between each click.

5, click each abstract page, make sure efetch is used, e.g.,

http://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pubmed&id=19501051&retmode=xml

http://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pmc&id=2708341

6, parse abstract using an XML parser.

Your apache error log, e.g., /var/log/httpd/white_board_ssl_error_log,
may have a line like this:
ModSecurity: Access denied with code 404 (phase 4).

To fix it, open your http virtual host file add this line:

e.g.,

Original Lyrics from  Les Miserables.

OLD BEGGAR WOMAN
What you think yer at?
Hanging round me pitch?
If you’re new around here, girl
You’ve got a lot to learn!

YOUNG PROSTITUTE
Listen you old bat…
Crazy bloody witch…
‘Least I give me customers
Some pleasure in return!

OLD BEGGAR WOMAN
I know what you give!
Give ‘em all the pox!
Spread around your poison
Till they end up in a box.

GeneExpresso and LipoExpress are from Lab Supply Mall
Lipofectamine 2000 is from Invitrogen.

Expressfect is from Denville Scientific, Inc. (http://www.denvillescientific.com), Expressfect is an OEM product (SoFast) from China (http://www.sunmabio.com, http://www.actgene.com/Convoy.htm).

Lipofectamine 2000	LipoExpress
GeneExpresso	Expressfect (SoFast)
Efficiency comparison of GeneExpresso, LipoExpress, Lipofectamine 2000, and Expressfect on Hela cells. A nuclear targeted GFP DNA was transfected with GeneExpresso, LipoExpress, Lipofectamine 2000, and Expressfect respectively per manufacturer’s protocols. The efficiency was checked 48 hours post transfection.
FACS Analysis

Conclusion:
Both GeneExpresso and LipoExpress have higher transfection efficiency than Lipofectamine 2000. Expressfect (OEM of SoFast) should not be used as transfection reagent.

Compared to EB which is a very strong mutagen, GRGreen caused fewer mutations than EB in the Ames test.

• Available at 10,000X in H₂O for better safety.
• Compatible with UV or blue light transilluminator and common gel documentation systems.
• Will not affect downstream experiments: compatible with all gel purification kits tested, will not inhibit ligation reaction etc.
• Compatible with Sodium Borate Electrophoresis Buffer: Run gel 2-3 times faster at higher voltage, resolve shaper bands in minutes, and less heat generation.
• Cut out DNA bands for subclonning under safer blue light: No mutations caused by EB and UV light.

Stain A	GRGreen
DarkReader Blue Light Transilluminator
Stain A	GRGreen
UV Transilluminator

 Fig. 2, GRGreen in water is more sensitive than Stain A in water.

1% agarose gel prepared with SeaKem LE Agarose (Lonza) and 1X GRGreen or 1X Stain A (from competitor A), run with 1X Lithium Borate Fast Electrophoresis Buffer at 100 V.

Left: 100 bp DNA ladder, 250ng, 125 ng, 62.5 ng and 31.3 ng, total ng DNA per lane.

Right: 1kb DNA ladder, 250ng, 125 ng, 62.5 ng and 31.3 ng, total ng DNA per lane.

TAE	TBE	Sodium Borate

Fig. 3, GRGreen is compatible with three common running buffers.

1% agarose gel prepared with SeaKem LE Agarose (Lonza) and 1X GRGreen, run with 1X  three Electrophoresis Buffers at 100 V.

Storage: Store at 4 ^oC.

Protocol:

1. Prepare 20 to 40 ml of agarose gel solution (concentration from 0.7~2.0%) with TAE, TBE or Borate Buffer in a 250 ml flask and mix it thoroughly. Place the flask in the microware, heat on high until the solution is completely clear and no small floating particles are visible (about 2~3 minutes).

2. After the gel solution cool to about 55 oC, add GRGreen to the solution to 1X final concentration. Swirl the flask gently to mix the solution and avoid forming bubbles.

3. Pour the gel solution into a gel tray until the comb teeth are immersed about 1/4~1/2 into the gel solution.

4. After the agarose gel has solidified you can perform electrophoresis using either 0.5 to 1X TAE, TBE or Borate Buffer.

5. Detect the bands using UV or blue light transilluminator.

FAQ

1, Should I wear gloves when using this dye?

   You should exercise common safe laboratory practices when using this reagent.

2, What blue light transilluminator should I used with GRGreen dye?

    DarkReader.

3, What filter should I use for blue light transilluminator?

    Filter from Clare Chemical.

 MSDS: GRGreen MSDS, Disposal Guide

Protocol

Figure 1. A Cartoon Showing Biodegradation of PolyExpress DNA Transfection Reagent After Endocytosis of Transfection Complex

Features

Bio-degradable after endocytosis

- Exceptional high titers of virus production

- Efficient for very long DNAs (>89 kb)

- Efficient for both single DNA transfection and multi DNA co-transfection

- High levels of recombinant protein production

- Simple and robust transfection procedure

- Very affordable

Storage Condition:  Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Broad Transfection Spectrum for Mammalian Cell Types

Examples Showing Transfection Efficiency of PolyExpress™ DNA In Vitro Transfection Reagent on Common Cell Lines

PolyExpress™	Lipofectamine Plus

Fig. 2, Transfection efficiency comparison of PolyExpress™ vs. lipofectamine Plus on Chinese Hamster Ovary (CHO) cells. HA tagged beta-tubulin cDNA was delivered into CHO cells with PolyExpress™ (left panel) and lipofectamine Plus (right panel) respectively. FITC conjugated antibody against HA tag was utilized to pick up HA-beta-tubulin (Green) while a DM1a antibody was used to detect endogenous alpha-tubulin followed by probing with rhodamine conjugated secondary antibody (Red). (Courtesy of Dr. Shang Yin, University of Texas at Houston Medical School).

PolyExpress™	Lipofectamine 2000

Fig. 3. A comparison showing transfection efficiency of PolyExpress™ reagent vs. a leading product, Lipofectamine 2000 on HEK293FT cells. HEK-293FT cells were transfected with GFP vector (pEGFP-N3) by PolyExpress™ (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

PolyExpress™	Lipofectamine 2000

Fig. 4. A comparison showing transfection efficiency of PolyExpress™ reagent vs. a leading product, Lipofectamine 2000 on HepG2 cells. HepG2 cells were transfected with GFP vector (pEGFP-N3) by PolyExpress™ (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection

PolyExpress™	Lipofectamine 2000

Fig. 5. A comparison showing transfection efficiency of PolyExpress™ reagent vs. a leading product, Lipofectamine 2000 on MDCK cells. MDCK cells are notoriously hard to transfect. With proprietary “Shaved Cell Transfection” protocol, PolyExpress™ (left panel) gives up to 70% GFP positive cells vs. Lipofectamine 2000 (right panel) around 5% efficiency. MDCK cells were transfected with GFP vector (pEGFP-N3) by PolyExpress™ (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 36 hours post transfection.

PolyExpress™	Fugene HD

Fig. 6. A comparison showing transfection efficiency of PolyExpress™ reagent vs. a leading product, Fugene HD on LNCap cells.  LNCap cells were transfected with GFP vector (pEGFP-N3) by PolyExpress™ (left panel) and Fugene HD (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection

PolyExpress™ DIC	PolyExpress™ FITC

Fig. 7. Neuro2A cells transfected with pEGFP-C1 plasmid using PolyExpress™ In Vitro DNA Transfection Reagent. The Neuro2A cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left) and FITC imaging (right) 24 hours post-transfection 

PolyExpress™	Lipofectamine 2000

Fig. 8. Comparison of cytotoxicity of PolyExpress ™ DNA In Vitro Transfection Reagent with L2K™ on primary murine skin fibroblast. The primary murine fibroblast was incubated with the indicated transfection reagents/pEGFP-C1 (DNA) complexes above for 4 hours in serum-free DMEM High Glucose medium followed by replacement of complete serum-containing medium. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging 24 hours post transfection