Nature Biotechnology is a monthly journal publishing new concepts in biological technology of relevance to bioengineering, medicine, energy, agriculture, food and the environment. It has a magazine covering the commercial, political, ethical, legal and societal aspects of this research. http://feeds.nature.com/nbt/rss/current Nature Publishing Group en © 2026 Macmillan Publishers Limited, part of Springer Nature. All rights reserved. Nature Biotechnology © 2026 Macmillan Publishers Limited, part of Springer Nature. All rights reserved. permissions@nature.com https://www.nature.com/uploads/product/nbt/rss.png http://feeds.nature.com/nbt/rss/current https://www.nature.com/articles/s41587-026-03126-z Nature Biotechnology, Published online: 11 June 2026; doi:10.1038/s41587-026-03126-z

A method for spatial CRISPR perturbation screening reveals how genetic changes alter coding and non-coding RNA in a native tissue context, including the tumor microenvironment.]]> Leon SchwartzPornchanan PholraksaYogesh Goyal doi:10.1038/s41587-026-03126-z Nature Biotechnology, Published online: 2026-06-11; | doi:10.1038/s41587-026-03126-z 2026-06-11 Nature Biotechnology 10.1038/s41587-026-03126-z https://www.nature.com/articles/s41587-026-03126-z https://www.nature.com/articles/s41587-026-03127-y Nature Biotechnology, Published online: 11 June 2026; doi:10.1038/s41587-026-03127-y

In vivo CRISPR genetic perturbations are spatially mapped at scale.]]> Alev BaysoyXiaolong TianPaul RenauerFeifei ZhangZhiliang BaiHao ShiMingyu YangDingyao ZhangMiao LiuHaikuo LiBo TaoArchibald EnninfulYao LuFu GaoGuangchuan WangWanqiu ZhangThao TranNathan Heath PattersonJie ShengShuozhen BaoChuanpeng DongShan XinBinfan ChenMei ZhongSherri RankinCliff GuyYan WangJon P. ConnellyShondra M. Pruett-MillerDaifeng WangMina XuMark B. GersteinHongbo ChiSidi ChenRong Fan doi:10.1038/s41587-026-03127-y Nature Biotechnology, Published online: 2026-06-11; | doi:10.1038/s41587-026-03127-y 2026-06-11 Nature Biotechnology 10.1038/s41587-026-03127-y https://www.nature.com/articles/s41587-026-03127-y https://www.nature.com/articles/s41587-026-03179-0 Nature Biotechnology, Published online: 10 June 2026; doi:10.1038/s41587-026-03179-0

Biotech is back. But continued investor caution, the rise of China and fast-moving AI are changing the sector’s contours.]]> Melanie Senior doi:10.1038/s41587-026-03179-0 Nature Biotechnology, Published online: 2026-06-10; | doi:10.1038/s41587-026-03179-0 2026-06-10 Nature Biotechnology 10.1038/s41587-026-03179-0 https://www.nature.com/articles/s41587-026-03179-0 https://www.nature.com/articles/s41587-026-03172-7 Nature Biotechnology, Published online: 09 June 2026; doi:10.1038/s41587-026-03172-7

Hybrid solid–liquid optics improve light-sheet imaging of intact biological samples.]]> Cheng GongPauline AffatatoMatt FaySudha R. GuttikondaNathan J. O’ConnorEmerson NobleMaci HealBenjamin HaydockRenee MapaEstanislao Daniel De La CruzGiacomo GattoniJohanna E. KowalkoMaria Antonietta ToschesCharles R. GerfenRene HenChristopher D. MakinsonHanina HibshooshJack R. GlaserRaju Tomer doi:10.1038/s41587-026-03172-7 Nature Biotechnology, Published online: 2026-06-09; | doi:10.1038/s41587-026-03172-7 2026-06-09 Nature Biotechnology 10.1038/s41587-026-03172-7 https://www.nature.com/articles/s41587-026-03172-7 https://www.nature.com/articles/s41587-026-03171-8 Nature Biotechnology, Published online: 08 June 2026; doi:10.1038/s41587-026-03171-8

We have developed single-cell spatial pharmacobiology (SSP), which combines in situ imaging of a systemically infused fluorescent therapeutic antibody with high-plex spatial proteomics. Applied to head and neck and pancreatic tumors from patients treated in phase 1 trials, SSP revealed marked spatial heterogeneity in antibody delivery and target engagement, which was shaped by conserved stromal barriers.]]> doi:10.1038/s41587-026-03171-8 Nature Biotechnology, Published online: 2026-06-08; | doi:10.1038/s41587-026-03171-8 2026-06-08 Nature Biotechnology 10.1038/s41587-026-03171-8 https://www.nature.com/articles/s41587-026-03171-8 https://www.nature.com/articles/s41587-026-03173-6 Nature Biotechnology, Published online: 08 June 2026; doi:10.1038/s41587-026-03173-6

Sub-zero technologies that reduce ice damage in organs could extend donor-graft shelf life — offering transplant centers more time to match recipients.]]> Andrew Marshall doi:10.1038/s41587-026-03173-6 Nature Biotechnology, Published online: 2026-06-08; | doi:10.1038/s41587-026-03173-6 2026-06-08 Nature Biotechnology 10.1038/s41587-026-03173-6 https://www.nature.com/articles/s41587-026-03173-6 https://www.nature.com/articles/s41587-026-03174-5 Nature Biotechnology, Published online: 05 June 2026; doi:10.1038/s41587-026-03174-5

Plant genome editing is multiplexed with twin prime editing.]]> Hongchao LiZhuangzhuang ChaiXiaoli ShiChao SunRui ZhangQiao ZhangZhenyu LiKang ZhangYuan LeiCaixia Gao doi:10.1038/s41587-026-03174-5 Nature Biotechnology, Published online: 2026-06-05; | doi:10.1038/s41587-026-03174-5 2026-06-05 Nature Biotechnology 10.1038/s41587-026-03174-5 https://www.nature.com/articles/s41587-026-03174-5 https://www.nature.com/articles/s41587-026-03162-9 Nature Biotechnology, Published online: 05 June 2026; doi:10.1038/s41587-026-03162-9

Folddisco enables protein structural motif search in million scale databases.]]> Hyunbin KimRachel Seongeun KimMilot MirditaJaewon YoonMartin Steinegger doi:10.1038/s41587-026-03162-9 Nature Biotechnology, Published online: 2026-06-05; | doi:10.1038/s41587-026-03162-9 2026-06-05 Nature Biotechnology 10.1038/s41587-026-03162-9 https://www.nature.com/articles/s41587-026-03162-9