Promega Profiles in DNA Articles

Career Opportunities

Tue, 08 May 2012 16:09:18 -0500

Promega Corporation is looking for an experienced scientist for an open position as a Forensic Regional Account Manager.

Evaluation of Haplotypes to Enhance the Power of STR Test Kits in Cases of Questioned Family Relationships

Fri, 09 Mar 2012 14:16:44 -0600

Scientists at Oklahoma State University determined that the use of FESFPS-Penta E haplotypes for parentage testing increased the discriminatory power of currently available test batteries in cases of disputed relatedness.

Book Review: The Killer of Little Shepherds

Tue, 07 Feb 2012 14:31:39 -0600

Ann MacPhetridge reviews Douglas Starr’s book The Killer of Little Shepherds, an account of Joseph Vacher, one of the worst serial killers in history, who terrorized the French countryside in the late 1890s, and the investigative efforts to capture him and bring him to justice.

Development of the PowerPlex® 21 System

Tue, 31 Jan 2012 16:00:55 -0600

The PowerPlex® 21 System is a five-color system that includes all materials needed for co-amplification and detection of 21 loci: D1S1656, D2S1338,D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51,D19S433, D21S11, Amelogenin, CSF1PO, FGA, Penta D, Penta E, TH01, TPOX and vWA.

Forensic Casework Sample Processing on the Maxwell® 16 Instrument

Thu, 26 Jan 2012 14:43:55 -0600

In this Technical Tips article, Paraj Mandrekar presents answers to a number of frequently asked questions about forensic casework sample processing on the Maxwell® 16 Instrument.

13th European Forensic DNA Working Group Meeting

Thu, 19 Jan 2012 14:37:56 -0600

The 13th European Forensic DNA Working Group Meeting will be held May 9 and 10, 2012, in Krakow, Poland.

Database Process Automation Using DNA IQ™, Plexor® HY and PowerPlex® 16 HS Systems on the Biomek® 3000 Workstation

Fri, 26 Aug 2011 09:34:17 -0500

In this article, Cyndi Cunnington from the Idaho State Police Forensic Services Laboratory describes the lab's experience to evaluate the Promega Identity Automation™ methods for DNA IQ™ extraction, Plexor® HY setup, sample normalization and PowerPlex® 16 HS amplification setup on the Beckman Coulter Biomek® 3000 workstation.

Allele Frequencies for Penta C in Caucasian, African American, American Indian, Asian and Hispanic Populations

Thu, 25 Aug 2011 10:29:40 -0500

This study presents Penta C allele frequencies for Caucasian, African American, American Indian, Asian and Hispanic populations.

Newest Members of the Genetic Identity Team

Mon, 18 Jul 2011 10:20:53 -0500

Promega introduces the newest Genetic Identity team members: Rohaizah James and Jon Drobac.

The Latest and Greatest NIST PCR-Based DNA Profiling Standard: Updates and Status of Standard Reference Material® (SRM) 2391c

Thu, 14 Jul 2011 16:16:20 -0500

In this article, NIST scientists introduce the new Standard Reference Material® 2391c (SRM 2391c) PCR-Based DNA Profiling Standard. This is the third renewal of this SRM, originally released in 1995 and consists of six components, labeled A through F.

New Control DNA for PowerPlex® Systems

Wed, 13 Jul 2011 16:27:46 -0500

Promega is replacing the 9947A DNA and 9948 Male DNA provided in PowerPlex® Systems with 2800M Control DNA beginning in 2012. This new control DNA is a single-source male human genomic DNA purified from blood. Because it is a male DNA, the control DNA is appropriate for use with all PowerPlex® Systems.

Improved Primer Pair for the SE33 Locus in the PowerPlex® ESI 17 Pro System

Tue, 12 Jul 2011 12:39:19 -0500

This article describes changes to the SE33 primer pair in the new PowerPlex® ESI 17 Pro System. This change allowed robust amplification of DNA samples containing the X.1 or X.3 SNP and allowed generation of amplicons that are not affected by the migration shift caused by these mutations. Also, the changes to this primer pair eliminated nonspecific amplification seen in some casework samples with the PowerPlex® ESI 17 System.

Forensic DNA Profiles Crossing Borders in Europe (Implementation of the Treaty of Prüm)

Fri, 08 Jul 2011 12:35:32 -0500

In this article, Kees van der Beek discusses the scope of the treaty of Prüm, its implementation status, the software used for the exchange of DNA profiles, Prüm inclusion and matching rules, different types of matches that are produced, the results obtained, prevention of false-positive matches and detection of false-negative matches.

Analysis of PowerPlex® Systems using the Applied Biosystems 3500 and 3500xL Genetic Analyzers

Wed, 16 Mar 2011 16:51:41 -0500

In this Technical Tips article, Dawn Rabbach answers your frequently asked questions about how to analyze PowerPlex® reactions on the new Applied Biosystems 3500 and 3500xL Genetic Analyzers.

Mixture Interpretation: Why is it Sometimes So Hard?

Wed, 16 Mar 2011 16:13:18 -0500

Interpretation of mixed DNA profiles can be simple and straightforward in some scenarios. However, certain factors (e.g., increasing relatedness or number of shared alleles, increasing the number of contributors or disparity in the amount of DNA from each source, an increase in degradation or presence of artifacts) will likely make mixture interpretation more complex. A combination of several factors generally confounds interpretation significantly.