Promega PubHub Articles

Simplifying Mechanistic Toxicity Testing Workflow with Automation

Mon, 14 May 2012 11:18:00 -0500

Differentiating Mitochondrial Toxicity from Other Types of Mechanistic Toxicity

Mon, 14 May 2012 08:52:57 -0500

Here we review the current state of the field and the available assays for detecting mitochondrial toxicity.

Doing Good Science: Authenticating Cell Line Identity

Fri, 11 May 2012 09:56:16 -0500

High-Content Live-Cell Imaging in New Dimensions

Thu, 10 May 2012 08:38:36 -0500

Scientists at Promega Corporation and PerkinElmer, Inc., combine the Promega HaloTag® protein reporter technology and PerkinElmer Opera® High Content Screening System and Operetta® High Content Imaging System for high-content analyses of large numbers of cells at subcellular resolution.

Profiling Compound Effects on Cell Health in a Time Course Using a Multiplexed Same-Well Assay

Mon, 07 May 2012 11:56:15 -0500

In this article, the authors highlight the dynamic nature of activity-based cytotoxicity biomarkers and the need for multi-parameter analysis to avoid misinterpreting a compound’s biological impact, which can happen when performing a single-time-point evaluation.

Efficient High-Throughput Protein Purification Using the Magne™ HaloTag® Beads

Wed, 18 Apr 2012 12:23:59 -0500

We developed an automated high-throughput pipeline for protein expression and purification on a Tecan Freedom® robotic platform using the Magne™ HaloTag® Beads.

Full-size Humor

Tue, 10 Apr 2012 09:22:14 -0500

FAQ for RT-qPCR

Fri, 06 Apr 2012 14:44:13 -0500

This article is a serries of questions and answers about RT-qPCR. It includes a glossery of terms and links to realated resources.

Getting the Best Results from Your Reverse Transcription Quantitative PCR Assay

Fri, 06 Apr 2012 10:26:59 -0500

This article describes the key considerations for reverse transcription (RT) qPCR.

Cell-Free Expression Systems to Study HDAC Protein Function

Tue, 27 Mar 2012 14:28:27 -0500

This article describes cell-free expression and capture of histone deacetylase (HDAC) proteins using the TnT® T7 Insect Cell Extract System, HaloTag® labeling and Maxwell® 16 isolation technologies.

Supplemental Information for RNA Quantification Using the GoTaq® RT-qPCR Systems

Mon, 26 Mar 2012 13:55:37 -0500

Information to accompany the PubHub article "RNA Quantification Using the GoTaq RT-qPCR Systems"

RNA Quantification Using the GoTaq® RT-qPCR Systems

Mon, 26 Mar 2012 13:18:22 -0500

This article demonstrates how well the GoTaq 1-Step and 2-Step RT-qPCR Systems perform and compare to four competitor one-step RT-qPCR systems.

Review: Interaction of 3ß-Hydroxysteroid Dehydrogenase with Mitochondrial Translocases

Wed, 14 Mar 2012 09:42:39 -0500

A paper describing use of the TNT® Reticulocyte Lysate System to synthesize substrates for mitochondrial import assays.

Review: Examining Complexity of Human RNA Polymerases Using HaloTag® Technology Coupled to Label Free Quantitative Proteomics

Tue, 13 Mar 2012 14:49:09 -0500

Review of an article published in the Journal of Proteome Research. The HaloTag Technology was used to examine RNA polymerases and their complexes. Techniques included protein pull-downs of unique interactors and cellular localization of the associated complexes.

Monitor Cell Viability and Reporter Gene Expression in the Same Well with the One-Glo™+Tox Luciferase Reporter and Cell Viability Assay

Wed, 15 Feb 2012 12:38:11 -0600

A brief article describing how a single-well assay can aid in interpretation of reporter assay data.