small RNA biochemistry

USB thumb drive, MinIon, that can read DNA directly from blood

Mon, 20 Feb 2012 19:51:00 -0800

Oxford Nanopore technologies: Sequence a human genome in 15 minutes & USB thumb drive, MinIon, that can read DNA directly from blood.

http://onforb.es/AdDZ2x

http://bit.ly/ys0OtF

http://bit.ly/AfJVR3

In addition to sequencing DNA, the GridION system is designed for the direct analysis of RNA strands. By customising the processive enzyme to be specific for RNA and adapting the nanopore to distinbuish RNA-specific bases, this RNA analysis system can be integrated into the GridION platform.

This system is designed to analyse the original sample RNA strand directly, rather than by undergoing conversion to cDNA. The simplification of workflow and direct - rather than surrogate - analysis is unique to nanopore sensing.

small RNA biochemistry

USB thumb drive, MinIon, that can read DNA directly from blood

Monsanto: too little experimental evidence that regulation of animal genes by plant microRNAs is an important dietary phenomenon

Technical Analysis: Zhang et al.

Monsanto Company

1/10/2012

Ingested plant miRNAs regulate gene expression in animals

Monsanto Co. from 2008: Numerous endogenous plant small RNAs have perfect complementarity to human genes & other mammals

Ago2 N domain is required for small RNA duplex unwinding but not for RISC loading or target binding & cleavage

Expression determinants of mammalian argonaute proteins in mediating gene silencing

Expression determinants of mammalian argonaute proteins in mediating gene silencing

Conserved Function of lincRNAs in Vertebrate Embryonic Development despite Rapid Sequence Evolution. Ulitsky et al.; Cell

Conserved Function of lincRNAs in Vertebrate Embryonic Development despite Rapid Sequence Evolution

Highlights

Summary

credit where credit is due: microRNA target mimicry and competing endogenous RNAs (ceRNAs)?

MAIN TEXT

REFERENCES

transcription of piRNA cluster in initiating de novo piRNA production against new transposon insertions

A role for transcription from a piRNA cluster in de novo piRNA production

Abstract

The 3′-to-5′ Exoribonuclease Nibbler Shapes the 3′ Ends of MicroRNAs Bound to Drosophila Argonaute1

The 3′-to-5′ Exoribonuclease Nibbler Shapes the 3′ Ends of MicroRNAs Bound to Drosophila Argonaute1

PABP is not essential for microRNA-mediated translational repression and deadenylation in vitro

PABP is not essential for microRNA-mediated translational repression and deadenylation in vitro

Abstract

Dicer is dispensable for asymmetric RISC loading in mammals.

Dicer is dispensable for asymmetric RISC loading in mammals

Abstract

Alternative miRNA Biogenesis

Alternative miRNA Biogenesis Pathways and the Interpretation of Core miRNA Pathway Mutants

Summary

human herpesvirus 6 (HHV-6) expresses a seed ortholog of the human miR-582-5p

Small RNA deep-sequencing identifies microRNAs and other small non-coding RNAs from human herpesvirus 6 (HHV-6B)

ABSTRACT

Animation of RNAi

a common oncogenic mechanism associated with altered DICER1 function that is selected during tumor developmen

Recurrent Somatic DICER1 Mutations in Nonepithelial Ovarian Cancers

the complexity of terminal modification pathways acting upon small regulatory RNAs

Common and distinct patterns of terminal modifications to mirtrons and canonical microRNAs

Abstract

mouse cytomegalovirus transcribes a microRNA-27 inhibitor upon infection

Argonaute protein identity and pairing geometry determine cooperativity in mammalian RNA silencing.

Argonaute protein identity and pairing geometry determine cooperativity in mammalian RNA silencing

Abstract

microRNA therapeutics; Gene Therapy Review