<?xml version='1.0' encoding='UTF-8'?><?xml-stylesheet href="http://www.blogger.com/styles/atom.css" type="text/css"?><feed xmlns='http://www.w3.org/2005/Atom' xmlns:openSearch='http://a9.com/-/spec/opensearch/1.1/' xmlns:georss='http://www.georss.org/georss' xmlns:thr='http://purl.org/syndication/thread/1.0' xmlns:gd='http://schemas.google.com/g/2005' gd:etag='W/&quot;C0ACQHw5cCp7ImA9WhRSFU8.&quot;'><id>tag:blogger.com,1999:blog-6930680727578028885</id><updated>2011-11-17T18:22:41.228+09:00</updated><category term='De novo sequencing'/><category term='iOS'/><category term='Computer Science'/><category term='Bioinformatics'/><category term='Biology'/><category term='Paper'/><title>Biology in Computational World</title><subtitle type='html'></subtitle><link rel='http://schemas.google.com/g/2005#feed' type='application/atom+xml' href='http://socoolbioinformatics.blogspot.com/feeds/posts/default'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default?redirect=false&amp;v=2'/><link rel='alternate' type='text/html' href='http://socoolbioinformatics.blogspot.com/'/><link rel='hub' href='http://pubsubhubbub.appspot.com/'/><author><name>SCB</name><email>noreply@blogger.com</email></author><generator version='7.00' uri='http://www.blogger.com'>Blogger</generator><openSearch:totalResults>9</openSearch:totalResults><openSearch:startIndex>1</openSearch:startIndex><openSearch:itemsPerPage>25</openSearch:itemsPerPage><entry gd:etag='W/&quot;C0EER3Y_fSp7ImA9WhRSFU8.&quot;'><id>tag:blogger.com,1999:blog-6930680727578028885.post-4779943971208627260</id><published>2011-11-17T18:19:00.000+09:00</published><updated>2011-11-17T18:20:06.845+09:00</updated><app:edited xmlns:app='http://www.w3.org/2007/app'>2011-11-17T18:20:06.845+09:00</app:edited><category scheme='http://www.blogger.com/atom/ns#' term='Paper'/><category scheme='http://www.blogger.com/atom/ns#' term='Biology'/><category scheme='http://www.blogger.com/atom/ns#' term='Bioinformatics'/><title>The age of a single cell assembly</title><summary>
The age of a single cell assembly has become. Every assembler will be reconstructed to focus on the single cell assembly.




Picking up the pieces: 
Picking up the pieces

Nature Methods 8, 896 (2011).            doi:10.1038/nmeth.1753

Author: Michael Eisenstein

An improved algorithm for genomic analysis allows scientists to build remarkably accurate and complete genomic sequences from single</summary><link rel='replies' type='application/atom+xml' href='http://socoolbioinformatics.blogspot.com/feeds/4779943971208627260/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/age-of-single-cell-assembly.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/4779943971208627260?v=2'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/4779943971208627260?v=2'/><link rel='alternate' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/age-of-single-cell-assembly.html' title='The age of a single cell assembly'/><author><name>SCB</name><email>noreply@blogger.com</email></author><thr:total>0</thr:total></entry><entry gd:etag='W/&quot;C0YDQX85fyp7ImA9WhRSFU8.&quot;'><id>tag:blogger.com,1999:blog-6930680727578028885.post-3794093816258935839</id><published>2011-11-17T18:07:00.000+09:00</published><updated>2011-11-17T18:12:50.127+09:00</updated><app:edited xmlns:app='http://www.w3.org/2007/app'>2011-11-17T18:12:50.127+09:00</app:edited><category scheme='http://www.blogger.com/atom/ns#' term='Paper'/><category scheme='http://www.blogger.com/atom/ns#' term='Biology'/><category scheme='http://www.blogger.com/atom/ns#' term='Bioinformatics'/><title>Annotation of the domestic dog genome sequence: finding the missing genes.</title><summary>
The sequencing the domestic dog genome with RNA-Seq technology!!



RNA-Seq.. Now, for genetic research, I don't know that RNA-Seq has to be implemented necessarily with NGS sequencing.



But, the money and time cost to analyze the data are the problem.




Annotation of the domestic dog genome sequence: finding the missing genes.: 




Annotation of the domestic dog genome sequence: finding </summary><link rel='replies' type='application/atom+xml' href='http://socoolbioinformatics.blogspot.com/feeds/3794093816258935839/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/annotation-of-domestic-dog-genome.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/3794093816258935839?v=2'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/3794093816258935839?v=2'/><link rel='alternate' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/annotation-of-domestic-dog-genome.html' title='Annotation of the domestic dog genome sequence: finding the missing genes.'/><author><name>SCB</name><email>noreply@blogger.com</email></author><thr:total>0</thr:total></entry><entry gd:etag='W/&quot;CkUMSHc4fCp7ImA9WhRSEkg.&quot;'><id>tag:blogger.com,1999:blog-6930680727578028885.post-430223409221664154</id><published>2011-11-14T14:38:00.001+09:00</published><updated>2011-11-14T14:58:09.934+09:00</updated><app:edited xmlns:app='http://www.w3.org/2007/app'>2011-11-14T14:58:09.934+09:00</app:edited><category scheme='http://www.blogger.com/atom/ns#' term='Computer Science'/><category scheme='http://www.blogger.com/atom/ns#' term='Bioinformatics'/><title>Spectra alignment</title><summary>


After k times shift, the algorithm to find common elements between two sets.


D(k) is the maximum common elements between two sets after k times shift.


[Example]

We assume that one shift is that each element, from 6th element to nth element, will subtract 5.

Set A = {10, 20, 30, 40, 50, 60, 70, 80, 90, 100}
Set B = {10, 30, 30, 40, 50, 55, 65, 75, 85, 95}
Set C = {10, 15, 30, 35, 50, 55, </summary><link rel='replies' type='application/atom+xml' href='http://socoolbioinformatics.blogspot.com/feeds/430223409221664154/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/spectra-alignment.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/430223409221664154?v=2'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/430223409221664154?v=2'/><link rel='alternate' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/spectra-alignment.html' title='Spectra alignment'/><author><name>SCB</name><email>noreply@blogger.com</email></author><media:thumbnail xmlns:media='http://search.yahoo.com/mrss/' url='http://2.bp.blogspot.com/-of1QKHUerdA/TsCtOW1FNHI/AAAAAAAAAFQ/mn6rX_glLJI/s72-c/spectra_alignment_1.png' height='72' width='72'/><thr:total>0</thr:total></entry><entry gd:etag='W/&quot;A0AASXY7fSp7ImA9WhRSEko.&quot;'><id>tag:blogger.com,1999:blog-6930680727578028885.post-10860845297927941</id><published>2011-11-14T11:14:00.001+09:00</published><updated>2011-11-14T23:09:08.805+09:00</updated><app:edited xmlns:app='http://www.w3.org/2007/app'>2011-11-14T23:09:08.805+09:00</app:edited><category scheme='http://www.blogger.com/atom/ns#' term='Paper'/><category scheme='http://www.blogger.com/atom/ns#' term='Computer Science'/><category scheme='http://www.blogger.com/atom/ns#' term='Bioinformatics'/><title>de Bruijn graph theory</title><summary>Recently, de Bruijn graph theory has widely used in the filed of genome assembly.

Expecially, Velvet assembler is based on this graph theory.

The goal of De Bruijn graph is to make superstring through overlapping k - 1 suffix sequence.

So this concept can well match with assembly of short reads of a genome.



de Bruijn graph sample (cited from Wikipedia)



How can de Bruijn graph make a </summary><link rel='replies' type='application/atom+xml' href='http://socoolbioinformatics.blogspot.com/feeds/10860845297927941/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/de-bruijn-graph-theory.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/10860845297927941?v=2'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/10860845297927941?v=2'/><link rel='alternate' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/de-bruijn-graph-theory.html' title='de Bruijn graph theory'/><author><name>SCB</name><email>noreply@blogger.com</email></author><media:thumbnail xmlns:media='http://search.yahoo.com/mrss/' url='http://3.bp.blogspot.com/-yM1u_rnsZOk/TsEY9s0IYAI/AAAAAAAAAFo/2BXgM-aghyc/s72-c/320px-De_bruijn_graph-for_binary_sequence_of_order_4.svg.png' height='72' width='72'/><thr:total>0</thr:total></entry><entry gd:etag='W/&quot;DUIERHsyeyp7ImA9WhRTGU0.&quot;'><id>tag:blogger.com,1999:blog-6930680727578028885.post-7842158105628002200</id><published>2011-11-10T15:45:00.000+09:00</published><updated>2011-11-10T15:45:05.593+09:00</updated><app:edited xmlns:app='http://www.w3.org/2007/app'>2011-11-10T15:45:05.593+09:00</app:edited><category scheme='http://www.blogger.com/atom/ns#' term='De novo sequencing'/><category scheme='http://www.blogger.com/atom/ns#' term='Bioinformatics'/><title>De novo assembly of single-cell bacterial genomes</title><summary>
In case of uncultured bacterial genome, NGS sequencers don't archive sequencing yet.



However, the multiplie displacement amplification (MDA, Go to Wikipedia and See this paper) method can be available.

MDA method is useful at the single cell but it might encounter amplification bias and chimeras.

Amplification bias is caused from the irregular coverage of amplified sequences and chimeras </summary><link rel='replies' type='application/atom+xml' href='http://socoolbioinformatics.blogspot.com/feeds/7842158105628002200/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/de-novo-assembly-of-single-cell.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/7842158105628002200?v=2'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/7842158105628002200?v=2'/><link rel='alternate' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/de-novo-assembly-of-single-cell.html' title='De novo assembly of single-cell bacterial genomes'/><author><name>SCB</name><email>noreply@blogger.com</email></author><thr:total>0</thr:total></entry><entry gd:etag='W/&quot;D0cERn4yeSp7ImA9WhRTF0k.&quot;'><id>tag:blogger.com,1999:blog-6930680727578028885.post-2711925623870355835</id><published>2011-11-08T18:36:00.001+09:00</published><updated>2011-11-08T18:36:47.091+09:00</updated><app:edited xmlns:app='http://www.w3.org/2007/app'>2011-11-08T18:36:47.091+09:00</app:edited><category scheme='http://www.blogger.com/atom/ns#' term='Computer Science'/><category scheme='http://www.blogger.com/atom/ns#' term='Bioinformatics'/><title>'AC' tag of Swissprot dat file</title><summary>I had known that 'AC' is unique like 'ID' in swissprot.

But, it was not true.

Moreover, there were multiple 'AC's in one 'ID'.

Oooops!!</summary><link rel='replies' type='application/atom+xml' href='http://socoolbioinformatics.blogspot.com/feeds/2711925623870355835/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/ac-tag-of-swissprot-dat-file.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/2711925623870355835?v=2'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/2711925623870355835?v=2'/><link rel='alternate' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/ac-tag-of-swissprot-dat-file.html' title='&apos;AC&apos; tag of Swissprot dat file'/><author><name>SCB</name><email>noreply@blogger.com</email></author><thr:total>0</thr:total></entry><entry gd:etag='W/&quot;DEcDQ3gycSp7ImA9WhRTFk0.&quot;'><id>tag:blogger.com,1999:blog-6930680727578028885.post-2765945972822452672</id><published>2011-11-07T03:59:00.000+09:00</published><updated>2011-11-07T04:01:12.699+09:00</updated><app:edited xmlns:app='http://www.w3.org/2007/app'>2011-11-07T04:01:12.699+09:00</app:edited><category scheme='http://www.blogger.com/atom/ns#' term='iOS'/><title></title><summary>





Slightly, I am having some clues for developing iOS application with Xcode.


Ha~.

But, I still feel much difficulties.</summary><link rel='replies' type='application/atom+xml' href='http://socoolbioinformatics.blogspot.com/feeds/2765945972822452672/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/slightly-i-am-having-clue-for.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/2765945972822452672?v=2'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/2765945972822452672?v=2'/><link rel='alternate' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/slightly-i-am-having-clue-for.html' title=''/><author><name>SCB</name><email>noreply@blogger.com</email></author><media:thumbnail xmlns:media='http://search.yahoo.com/mrss/' url='http://2.bp.blogspot.com/-HTEuabAbQmw/TrbY8fsCenI/AAAAAAAAADE/MkKw3jzfAmM/s72-c/ios_sample.png' height='72' width='72'/><thr:total>0</thr:total></entry><entry gd:etag='W/&quot;CUYDRH05cSp7ImA9WhRTFEo.&quot;'><id>tag:blogger.com,1999:blog-6930680727578028885.post-5274132600300196088</id><published>2011-11-05T15:03:00.001+09:00</published><updated>2011-11-05T15:06:15.329+09:00</updated><app:edited xmlns:app='http://www.w3.org/2007/app'>2011-11-05T15:06:15.329+09:00</app:edited><category scheme='http://www.blogger.com/atom/ns#' term='iOS'/><title>Start iOS development</title><summary>With mac mini, I started  iOS application development.

Unlike other GUI programming, I am feeling that it's too hard.

Actually, xcode development toolkit is not intuitive.

Maybe I am not good at using xcode.



</summary><link rel='replies' type='application/atom+xml' href='http://socoolbioinformatics.blogspot.com/feeds/5274132600300196088/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/start-ios-development.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/5274132600300196088?v=2'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/5274132600300196088?v=2'/><link rel='alternate' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/start-ios-development.html' title='Start iOS development'/><author><name>SCB</name><email>noreply@blogger.com</email></author><media:thumbnail xmlns:media='http://search.yahoo.com/mrss/' url='https://lh3.googleusercontent.com/-Y5H8VSADgMY/TrTSVKRGfJI/AAAAAAAAAC8/XYkVwYQj2E4/s72-c/blogger-image--1636777739.jpg' height='72' width='72'/><thr:total>0</thr:total></entry><entry gd:etag='W/&quot;A0UMQXs4fSp7ImA9WhRTEko.&quot;'><id>tag:blogger.com,1999:blog-6930680727578028885.post-3831651206596523462</id><published>2011-11-02T15:39:00.000+09:00</published><updated>2011-11-03T09:14:40.535+09:00</updated><app:edited xmlns:app='http://www.w3.org/2007/app'>2011-11-03T09:14:40.535+09:00</app:edited><category scheme='http://www.blogger.com/atom/ns#' term='Paper'/><category scheme='http://www.blogger.com/atom/ns#' term='Computer Science'/><title>The PatchMatch Randomized Matching Algorithm for Image Manipulation | November 2011 | Communications of the ACM</title><summary>
Ho....








The PatchMatch Randomized Matching Algorithm for Image Manipulation | November 2011 | Communications of the ACM
</summary><link rel='replies' type='application/atom+xml' href='http://socoolbioinformatics.blogspot.com/feeds/3831651206596523462/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/patchmatch-randomized-matching.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/3831651206596523462?v=2'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/6930680727578028885/posts/default/3831651206596523462?v=2'/><link rel='alternate' type='text/html' href='http://socoolbioinformatics.blogspot.com/2011/11/patchmatch-randomized-matching.html' title='The PatchMatch Randomized Matching Algorithm for Image Manipulation | November 2011 | Communications of the ACM'/><author><name>SCB</name><email>noreply@blogger.com</email></author><media:thumbnail xmlns:media='http://search.yahoo.com/mrss/' url='http://4.bp.blogspot.com/-ryWS2JctW8o/TrDl7A4m_rI/AAAAAAAAACI/M6Z0J6M_RLg/s72-c/The+PatchMatch+Randomized+Matching+Algorithm+for+Image+Manipulation+-+November+2011+-+Communications+of+the+ACM.png' height='72' width='72'/><thr:total>0</thr:total></entry></feed>