Kinetochore-localized KMN complexes mediate chromosome segregation by attaching chromosomes to spindle microtubules. Cryo-EM, biochemical, and genetic experiments reveal conserved underlying mechanisms of complex assembly, auto-inhibition, and phosphoregulation that ensure precise control of KMN assembly onto centromeres to enable genome division.

Fan and Yang et al. reveal that the SWR1 chromatin remodeling complex plays a crucial, noncanonical role in preventing the vacuolar delivery of autophagy-related proteins in yeast. This work uncovers a novel link between chromatin remodeling and autophagy dynamics, highlighting the importance of the Atg21-Rvb1 module in this process.

This study reveals that cells in moving tissues strengthen their connections by actively shuttling adhesion proteins along cell edges to vertices, enabling rapid responses to mechanical forces. This transport depends on proteins existing in an optimal physical state, like damp sand, that can be modulated by physiological temperature changes, offering new insights into how tissues maintain integrity during development.

Yang et al. perform a family-wide analysis of RhoGAP and RhoGEF localization and function during Drosophila border cell migration and identify RhoGAP15B as a key regulator of actomyosin dynamics. By limiting RhoA–myosin contractility in leading cells, RhoGAP15B stabilizes protrusions and coordinates cytoskeletal dynamics for efficient collective migration.

Whether and how individual cells within a tissue cooperate with each other to respond to cellular stress has remained elusive. Nandakumar et al. show that Drosophila adipocytes transport select subcellular components across intercellular bridges with their neighbors, and that such transport is required for tissue resilience to various stressors.

Marescal et al. show that quiescent cells disassemble their centromeres through the transcriptional downregulation of most centromere proteins while preserving those required to maintain centromere identity. During quiescence exit, the centromere is reassembled during S phase, but CENP-A is only redeposited in the following G1 after mitotic exit.

Zhao et al. reveal that PI(3)P-dependent recruitment of FGD at endosome–mitochondrion contacts activates CDC42 to regulate mitochondrial networks through the actin organization and DRP-1 in Caenorhabditis elegans.