Can you tell us about your background and what you work on now?

I’m trained as a developmental plant biologist and did my PhD and postdoc at New York University, USA. I’m also a multidisciplinary artist and have always had creative projects going alongside my research, working across graphic design, motion graphics/animation, music, and video art. I just relocated from New York to London to focus more fully on creative work.

Were you always going to be a scientist? And what about art – have you always enjoyed it?

As a child, I really wanted to be an artist. To try to  and encourage me to get more into science, my parents would get me these science coloring books on biology, astronomy, etc. Those books really informed my love of the synthesis of art and science. When I got interested in science again, towards the end of my undergraduate studies, it was really through a love of the figures and the aesthetics of science graphics. The creative problem-solving and hands-on nature of benchwork and experimental biology were also always really appealing and rewarding to me. But a PhD and a postdoc later, my favorite part of research is still the visuals.

What or who are your most important artistic influences?

I’m equally drawn to precision and noise. On the one hand, I love the clean line art of technical drawings, manuals and other documentation, scientific and medical textbook diagrams, and architectural drawings. At the same time, I love experimental media of all kinds: works by people at the margins of their fields, outsider art, non-linear storytelling, purely textural and abstract pieces, horror B-movies, and high-concept works that don’t take themselves too seriously. Trying to integrate those extremes, in ways both dramatic and understated, motivates a lot of my creative work. For example, in a recent project for Pioneer Works Science Studios, I got to combine a 2D line-art animation style with more abstract textures I made using my video synthesizer.

I struggle with favorites, but here are a few works I’ve enjoyed recently: The Wax Child by Olga Ravn, the architecture of the Kyoto International Conference Center, the fresh-mint ice cream from Towpath in London (it tastes like actual mint!), and Good Morning Extra! by Water With Water.

How do you make your art?

For my graphic design projects, I primarily work in Figma, Illustrator, and InDesign. I use After Effects for motion graphics, 2D animation, and compositing. I also use 3D software for a lot of my static and animated work, but render everything using toon shaders to give it all a technical line-art feel. Lately, I’ve been experimenting with interactive animations using Rive and developing interactive biology learning materials. I make my video art using analog modular video synthesizers.

Does your science influence your art at all, or vice versa, or are they separate worlds?

They definitely mutually reinforce one another. The meticulous, organized, and cross-disciplinary nature of science lends itself well to the pixel-perfect and collaborative demands of graphic design. And being able to think in terms of systems, parts, and processes helps with the procedural nature of making art using analog video or other node-based workflows.

What are you thinking of working on next?

On the personal side, I am working on an ongoing video art series called post-CAD that sits somewhere between postcards, graffiti, and thrift store re-paintings. Outside of my visual art practice, I play in the art rock duo Tar Of and the SWANA electronic duo Googoosh Dolls, and co-organize the annual technowruz series in NYC. We are about to mix a new Tar Of album so that’s next on my plate as well.

Professionally, I’m currently looking for opportunities where I can focus more fully on creative work informed by my science background, either in-house somewhere or within a studio or agency.

How/ where can people find more about you?

You can find me on Instagram @raminrahni and see more of my work at raminrahni.com 

The post SciArt Profile: Ramin Rahni appeared first on the Node.

In this quarterly webinar series, hosted by Pablo J. Sáez and Valeria Venturini, we aim to showcase the latest research in the field, and we’d love to hear from researchers (especially early-career researchers) who would like to present their work. You can get in touch with us at focalplane@biologists.com or fill in our short application form.

The post New FocalPlane features… webinar series on cell migration appeared first on the Node.

Development, host of the Node, invites you to submit your latest research to our upcoming Special Issue – Plant and Algae Development. This issue will be coordinated by Academic Editor Dominique Bergmann (Stanford University, USA) and Guest Editor Susana Coelho (Max Planck Institute for Biology, Tübingen, Germany).

Developmental biology aims to understand how a single cell, the zygote, becomes a complex multicellular organism with specialised cell types organised into functional units. Derived from unicellular ancestors, algae, plants and animals use shared developmental principles, such as biophysical interactions, signalling, patterning and cell fate determination, to innovate and overcome obstacles for multicellular development, such as coordinating growth. The highly plastic development of photosynthetic organisms have long offered specific opportunities to answer questions regarding the origins of multicellularity, stem cell maintenance and regeneration, as well as environmental and microbial interactions and integration. In recent years, advances in genomics, imaging and synthetic biology are revealing how gene regulatory networks, mechanical biology and signalling interact to shape form and function in plants and algae. Furthermore, mathematical, computational and modelling approaches combined with traditional experimental biology have revealed, explained and predicted quantifiable properties behind phenotypes and across scales. We also see an exciting range of research organisms in use today, including species of algae, moss, ferns, flowering plants and others. In this special issue, we aim to showcase the contribution of plants and multicellular algae to our understanding of development, regeneration and evolution, highlighting quality research across the entire breadth of developmental biology.

The deadline for submitting research papers is 2 November 2026.

The post Call for papers – Plant and Algae Development appeared first on the Node.

The meeting will be held at the Francis Crick Insitute on the 15th June 2026.

There is still time to register to attend the conference!

The deadline for our imaging competition has been extended until 10^th June so that everyone can submit their amazing scientific images!

The competition details are below and on the submission form. Link: https://docs.google.com/forms/d/e/1FAIpQLScPPGh6YvNrS7Pbe79E5OxEb6lSg8yhpDdJuHARpbpBjBfVZg/viewform?usp=dialog

Note: it appears that some people are having issues with google forms, apologies if this has affected you. If you cannot use the form, please email your entry to us (see below for what is needed) and we will add it for you!

Looking forward to seeing all your images!

Many thanks,

Timothy

On behalf of YEN committee

Competition details:

• Submit an original image you have generated related to developmental or stem biology. This may be a microscopy image, an image analysis output, or an artwork inspired by your scientific work.
• Provide a title and a short description of the image, explaining the scientific context and techniques used (e.g. microscopy method or analysis strategy).
• Provide your full name and affiliation. This information will be used for our internal records and future acknowledgement, but will not be displayed during voting. 

You can view examples from the YEN 2025 Image Competition here.

Terms and Conditions

By sending us an image for the YEN Image Competition, you agree to the YEN Committee displaying the image publicly (including on our website and social media) and using it on YEN materials to promote future events. We will always credit you unless you request otherwise.

All submissions will undergo an initial review by the YEN Committee based on quality and visual impact. Applicants will be notified of the outcome of this selection through the email address provided in this form.  

The post YEN 2026 Conference and Image Competition appeared first on the Node.

What makes IMEV special is not only its location on the French Riviera, but also the scientific history embedded in the place itself. Since the late 19th century, researchers have come to Villefranche-sur-Mer to study marine embryos, fertilization, plankton, and development; taking advantage of the bay’s extraordinary biodiversity and direct access to living marine organisms. In many ways, modern marine developmental biology was shaped in places like this.

And you still feel that spirit today.

Although IMEV is relatively small, it is well equipped and highly efficient for experimental work (Fig. 1).

Fig. 1. Institut de la Mer de Villefranche (IMEV), Villefranche-sur-Mer, France.

Access to marine organisms, imaging platforms, and technical support made it possible to rapidly test several GCaMP variants and explore live imaging approaches in sea urchin larvae (Fig. 2).

Fig. 2. Calcium imaging in the sea urchin larva Paracentrotus lividus expressing GCaMP6s.
A. Brightfield image overlaid with GCaMP6s fluorescence signal (green). B – B’. MAX projections from selected time windows of an XYT recording, showing representative spontaneous calcium activity patterns. Fluorescence intensity is displayed using the Fire (LUT).

A key part of what made this project so productive was being hosted by Luis Bezares Calderon.

I had never met Luis before this visit. I knew his work on neuronal and behavioral mechanisms in Platynereis, so during the proposal stage I sent him a simple email asking whether he would consider hosting me at IMEV. He generously agreed.

From the beginning, he gave me full access to his lab resources and created a highly collaborative environment. But what shaped the experience most was not only the experimental support, it was the way he constantly challenged the thinking behind the experiments.

His questions were persistent and fundamental: Why this approach? How will it answer your question? Are you just going to look at traces forever, or understand their function?

Coming from a molecular biology background, I was used to thinking mainly in terms of genes, molecules, and cellular mechanisms. Working with Luis pushed me to think more directly about nervous system function and behavior. For the first time in my life as a researcher, I witnessed behavioral assays in sea urchin larvae with my own eyes while simultaneously trying to connect activity dynamics to biological function.

This connection between molecules, cells, neural activity, and behavior is something Luis actively builds into his science. I found that intellectually challenging, stimulating, and genuinely exciting.

This experience reminded me that marine stations are not just places to run experiments. They are environments where questions can evolve quickly because of access, collaboration, and the intensity of scientific interaction.

I am very grateful to everyone at IMEV, and especially to Luis, for making this possible.

The post What can happen during a short stay at a marine station? appeared first on the Node.

This question has followed me for more than a decade. Not as a single project, but as a thread that kept resurfacing—each time forcing us to rethink what we thought we understood.

The idea of comparing regenerative zebrafish with non-regenerative medaka started as one of several proposals I discussed with Didier Stainier when I joined his lab in 2014. At the time, it felt simple: let biology provide the contrast, instead of trying to extract answers from a single system.

Zebrafish regenerate their hearts. Medaka do not. That difference was too striking to ignore (1).

I was in my second postdoc then, aware that I needed something I could carry forward. Didier encouraged me to pursue it, even though it was clearly high-risk. Looking back, that decision shaped everything that followed.

When the data pointed to something unexpected

Together with Rubén Marín-Juez —who later became both a key collaborator and a close friend—we established a cryoinjury model in medaka and generated our first comparative RNA-seq datasets (2).

What we saw was not what we expected.

Instead of cardiomyocyte-centered differences, which at the time were widely viewed as the primary drivers of cardiac regeneration, the strongest signals pointed toward immune responses and angiogenesis (2). I remember hesitating. It felt like we were drifting away from what many would consider the “core” of cardiac regeneration.

But the data were clear, and we decided to follow it.

We used to joke that we were like the Maze Runners—moving forward without knowing what was coming next, or how things would end. Rubén focused on angiogenesis and uncovered how revascularization is an early and essential step in regeneration (3, 4). In parallel, I moved toward immunity—despite being warned, quite accurately, that “immunity is too complicated and messy to work with.”

In hindsight, that hesitation reflects something broader. Work in non-mammalian models is often judged by how directly it translates to human biology, rather than by the clarity of the biological principles it can reveal. Yet it is precisely these systems—and the people willing to pursue them—that allow us to uncover mechanisms that are otherwise difficult to see.

A result we thought we understood—but didn’t

What stood out early was that macrophage infiltration appeared delayed and reduced in medaka (2). So, we asked a simple question: what happens if we delay macrophage recruitment in zebrafish?

Using clodrosome, we transiently depleted macrophages prior to injury and observed impaired regeneration.

At the time, we interpreted this primarily as a timing effect—an early delay that irreversibly disrupts regeneration.

This seemed to fit the data. Macrophages eventually came back, and their numbers recovered within about a week. Yet the heart still failed to regenerate.

We moved forward with that explanation.

But it never fully made sense.

Revisiting the problem with better resolution

It was only years later, after I started my lab at the Institute of Biomedical Sciences at Academia Sinica in Taiwan, that we revisited this question with better tools.

Through temporal single-cell profiling—driven in large part by the careful and persistent work of Ke-Hsuan Wei, one of the first PhD students in my lab—we realized something we had completely missed before: clodrosome was not simply delaying macrophages—it was preferentially depleting the resident macrophage population (5).

These cardiac resident-like macrophage subsets turned out to be essential for heart regeneration—coordinating revascularization, cardiomyocyte survival, debris clearance, and extracellular matrix remodeling.

That realization reframed everything.

Even when we allowed extended recovery, giving circulating, monocyte-derived macrophages ample time to repopulate the heart, regeneration did not recover.

At that moment, the entire story finally made sense.

It was not only about timing. It was also about identity.

What initially appeared to be a delay in macrophage function was, in fact, the loss of a specific and irreplaceable cell population.

Turning a non-regenerative system on

We then asked the opposite question: instead of suppressing the immune response, could activating it change the outcome?

Poly I:C—identified through comparative transcriptomics—enhanced macrophage recruitment and, unexpectedly, enabled de novo regeneration in medaka (2).

That was one of those moments when you don’t immediately trust the data. We repeated the experiments, trying to convince ourselves it wasn’t an artifact.

But it held.

Regenerative capacity began to look less like a fixed property and more like something that could be modulated.

From immune identity to regenerative signal

Our recent paper in PNAS represents the latest step in this progression (6).

Led by Kaushik Chowdhury, this phase of the work brought together comparative analysis, single-cell profiling, and functional experiments to identify a regeneration-associated macrophage population induced by poly I:C.

These macrophages localize to the injury border zone and express Granulin.

What started as a candidate marker became a functional insight. Through a series of carefully executed experiments, the team showed that recombinant Granulin alone is sufficient to promote cardiomyocyte proliferation and reduce scarring—linking immune activation to a concrete regenerative outcome.

Closing the loop, granulin expression is also activated in zebrafish following cardiac injury and is essential for heart regeneration.

Basic science, unexpected translation

I always consider myself a basic scientist and a developmental biologist. None of this work started with a translational goal.

It was driven by curiosity—by a question that seemed fundamental, but not immediately “useful” or “applicable”.

And yet, it led us to a concept that is inherently translational: that regeneration might be induced through immune modulation.

As Didier once put it:

“One never really knows when a basic science finding will transform translational research… CRISPR/Cas9 is just one recent example.”(7)

That perspective has stayed with me throughout this journey. Especially at moments when the work felt uncertain, or when its relevance was not immediately obvious.

Still the same question

If there is one idea that has gradually emerged, it is that regenerative capacity is not fixed.

It is governed, at least in part, by the identity and function of immune cells—and therefore potentially modifiable.

That doesn’t make the problem simple. But it reframes it.

And in many ways, we are still following the same question.

Just with a clearer understanding of what actually matters—and with a team that made it possible to see it.

References

1.     Ito K, Morioka M, Kimura S, Tasaki M, Inohaya K, Kudo A. Differential reparative phenotypes between zebrafish and medaka after cardiac injury. Developmental Dynamics. 2014;243(9):1106-15.

2.     Lai S-L, Marín-Juez R, Moura PL, Kuenne C, Lai JKH, Tsedeke AT, et al. Reciprocal analyses in zebrafish and medaka reveal that harnessing the immune response promotes cardiac regeneration. eLife. 2017;6:e25605.

3.     Marin-Juez R, Marass M, Gauvrit S, Rossi A, Lai S-L, Materna SC, et al. Fast revascularization of the injured area is essential to support zebrafish heart regeneration. Proceedings of the National Academy of Sciences. 2016;113(40):11237-42.

4.     Marín-Juez R, El-Sammak H, Helker CSM, Kamezaki A, Mullapuli ST, Bibli S-I, et al. Coronary Revascularization During Heart Regeneration Is Regulated by Epicardial and Endocardial Cues and Forms a Scaffold for Cardiomyocyte Repopulation. Developmental Cell. 2019;51(4):503-15.e4.

5.     Wei K-H, Lin IT, Chowdhury K, Lim KL, Liu K-T, Ko T-M, et al. Comparative single-cell profiling reveals distinct cardiac resident macrophages essential for zebrafish heart regeneration. eLife. 2023;12:e84679.

6.     Chowdhury K, Huang C-L, Lin IT, Hung Y-J, Lim KL, Liu H-W, et al. Immune modulation promotes heart regeneration through macrophage and Granulin functions in medaka. Proceedings of the National Academy of Sciences. 2026;123(16):e2524705123.

7.     Grewal S. An interview with Didier Stainier. Development. 2015;142(17):2861-3.

The post From comparison to mechanism: decoding heart regeneration appeared first on the Node.

The post The case for group meetings (all of them) appeared first on the Node.

Research laboratory presentation

The Marín-Juez laboratory, at the CHU Sainte-Justine Research Center, is recruiting PhD students and postdoctoral fellows (up to 5 years fully funded position).  Our laboratory is interested in the cellular and molecular mechanisms regulating cardiac regeneration and development.  

The successful applicant will join the Marín-Juez laboratory at the CHU Sainte-Justine Research Center, where they will have access to state-of-the-art facilities and technology platforms including Advanced imaging platform (light-sheet, spinning-disc confocal, multiphoton, STED super-resolution, etc.), genomics (DropSeq, 10x, Illumina Novaseq, Visium), IPSC Cell Reprograming and bioinformatics platforms.  The CHU Sainte-Justine Research Center provides a thriving scientific environment where the successful applicant will have the opportunity to work with multidisciplinary scientific teams and to collaborate with talented clinicians and researchers. 

Research project description

We have previously uncovered mechanisms governing coronary network replenishment, including the formation of a vascular scaffold that supports cardiomyocyte regeneration and mediates coronary-epicardial interactions and immune responses (Marín-Juez et al., PNAS 2016; Marín-Juez et al., Dev Cell 2019; El-Sammak et al., Circ Res 2022; Wang et al., Development 2024; Gupta et al. Dev Bio 2025; Rouf et al. Development 2026). Our recent work identifies the epicardium as a master regulator of cardiac fibrosis resolution and tissue replenishment (Kayman-Kürekçi et al. NCVR2026).

Building on these findings, we now aim to elucidate how the cardiac endothelium, epicardium, and immune system components cooperate to regulate tissue replenishment, as well as the specific mechanisms underlying their roles in cardiomyocyte regeneration and development.

PhD student position 

Applicants should have training in molecular biology, cell biology, or related fields.  Candidates should be enthusiastic about regenerative and developmental biology. Previous research experience with zebrafish and/or heart regeneration is highly valued but not essential. Candidates with experience in confocal/light-sheet imaging and/or genome engineering are strongly encouraged to apply.

Postdoc position 

We are looking for candidates with a Ph.D. in the biological sciences and laboratory experience in tissue repair/regeneration, cellular, molecular biology, or genetics.  Previous experience working with zebrafish, imaging and histology are highly valued but not essential. Candidates with experience in confocal/light-sheet imaging and/or genome engineering are strongly encouraged to apply.  Preference will be given to applicants with excellent collaborative and communication skills.  

How to apply

Candidates must send the required documentsto Rubén Marín Juez at ruben.marin.juez.hsj@ssss.gouv.qc.ca

Please provide:

• Curriculum vitæ
• Cover letter 
• References (2 or 3)

The post Molecular control of cardiac regeneration and repair appeared first on the Node.

Our laboratory uses human and mouse embryonic stem cells (ESCs) as well as mouse embryos to understand the molecular mechanisms underlying cell fate decisions. The applicant should have or be about to have a PhD in Developmental Biology, Genetics, Molecular Biology or similar, and must have demonstrated expertise in mouse early embryology (mouse pre-implantation embryo isolation and in vitro mouse embryo culture). Expertise in embryo microinjection and manipulation will be considered an advantage. In addition, molecular biology/mammalian cell culture (preferably in embryonic stem cells) and knowledge of next generation sequencing technologies will also be relevant for the position.

The applicant will be involved in a variety of exciting projects studying cell plasticity/totipotency to explore the underlying mechanisms of new regulators of Zygotic Genome Activation. We seek highly motivated, creative individuals, eager to learn and develop new technologies and complex cell systems based on live cell/embryo imaging, single-cell technologies and CRISPR-based editing, interested in understanding how a single cell can develop into a complex multicellular organism in vitro and in vivo.

Please send a brief cover letter and the names of three references via e-mail to:

Email: sergio.ruizmacias@nih.gov

Laboratory web: https://ccr.cancer.gov/Laboratory-of-Genome-Integrity/sergio-ruiz-macias

Recent publications from the lab:

• Vega-Sendino, et al (2021) The ETS Transcription Factor ERF controls the exit from the naïve pluripotent state. Sciences Advances, 7(40): eabg8306.
• Olbrich, T., et al (2021) CTCF is a barrier for 2C-like reprogramming. Nature Communications 12, article number: 4856.
• Vega-Sendino, M., et al (2024) The homeobox transcription factor DUXBL controls exit from totipotency. Nature Genetics, 56, 697–709.
• Saykali, B., et al (2025) Lineage-specific CDK activity dynammics characterize early mammalian development. Cell Reports, 44: 115558.

The post Postdoctoral position in Embryonic Stem Cell Biology and Early Embryonic Development appeared first on the Node.

Normally, at The Company of Biologists (Development’s publisher), we follow a hybrid working model, which means that we work from home for half of the week (which is usually 2-3 days a week for me) and the rest in the office. If you haven’t seen a photo of our office building yet, it is a rather beautiful building, combining the charm of a cottage-style exterior (complete with hipped roofs and classic sash windows) with a bright, modern and open-style office inside.

At Development, Ingrid Tsang and I are the Reviews Editors and we mainly handle the journal’s front-section content (so, that includes Reviews, Spotlights, Perspectives, Hypotheses, Primers, interviews – yeah, we have an extensive list!) and we work closely with Alex Eve, Executive Editor of Development.

I normally start my workday between 9:30-10 am, and my first task is always to reply to emails while sipping my morning coffee (a strong flat white if I am home and a long cappuccino when in the office).

After the first half an hour to attending to emails regarding submissions, chasing authors for their submissions, or finishing off a pending task from the previous day (which often involves taking a final read through a decision letter), I move on to the main tasks of the day.

If I am working on a chunky edit – meaning a developmental edit of a Review article – I would usually block off an entire day for it (at least 7–8 hours). This typically happens once a Review-type article (which we commission in-house and invite authors to submit) has gone through peer review. At that stage, we, the in-house Reviews Editors, read through the full manuscript in detail, commenting on scientific accuracy, language and structure, conciseness and accessibility, journal style, article length and references – all while helping authors address the Reviewers’ comments more effectively. I also go through the figures and legends (we take our display items very seriously, as a single figure often speaks a thousand words), commenting on visual appeal, labelling and other finer details. Developmentally editing an article is usually the most rigorous part of the job, at least in my opinion, as it ensures that the final piece is not only of high quality but also forward-looking and engaging for our wide readership. At Development, we pride ourselves on being extremely hands-on when guiding authors and helping them address both our feedback and the Reviewers’ comments, in order to publish the best possible version of their review.

Another crucial part of our job is commissioning. We have our in-house commissioning meetings every two weeks. So, if you catch me the afternoon before, I am usually frantically reading articles on a certain topic of interest, trying to prepare somewhat cohesive pitches to discuss with the rest of the team. We mainly invite authors to write peer-reviewed, review-type content for us. To identify emerging topics in the field, we attend important conferences, chat with researchers across a wide range of developmental biology disciplines, keep an eye on their websites, analyse research trends across primary research articles and participate in extensive 1-1.5-hour long commissioning meetings.

Once we have agreed on a topic and a suitable author, we invite them to write for us. If they accept our invitation, the author will then often involve their students and collaborators as co-authors, and at that point we discuss the potential scope and type of the article. Of course, we also have a thorough in-house pipeline that monitors the status of all articles from invitation through to acceptance.

When we’re in the office, Alex, Ingrid, Andrea (Community Manager of the Node) and I often chat about various aspects of the job throughout the day – both formally and informally – because our work requires teamwork and collaboration. So, Ingrid and I will often discuss scheduling to make sure our publication pipeline stays tight (i.e. that every Issue publishes a few front-section content). If we have just returned from a conference, we chat with Alex and Andrea about emerging research trends and potential blogs/ posts for the community site. We also bounce around ideas for commissioning topics and share feedback on each other’s pitches, amidst a healthy dose of random life chats.

For me, the day usually ends with a quick catch-up on plans for the next day. This is also when I respond to any remaining email replies from the morning. I usually like to do a final run-through of my to-do list, ticking things off and marking any pending tasks (if there are any). And with that, I sign off for the day!

PS: Ingrid has also written a piece on a day in the life of a Reviews Editor – so do give that a read as well!

The post What does a Reviews Editor do? appeared first on the Node.

Of course, it’s not just me. Saanjbati – my partner in crime on the Reviews Editors team – has also been fielding these questions since she started this job as well. So, given the appetite from ECRs in hearing about our jobs, we’re lifting the lid on the elusive title of ‘Reviews Editor’ to show you what really goes on behind the scenes to deliver Development’s review articles and other front section content. To kick things off, Saanjbati has an article on “What does a Reviews Editor do?” and I am providing a run-down here of one sunny (!) day in April 2026, randomly chosen by my prettiest d20 die for your perusal.

It’s probably worth noting here that, as with most jobs, each day in the journal’s office is very different to the next. So do let me know if you would like to see another day in my life. But for now, hope you enjoy reading about this one!

Whilst I am writing about my experiences as a Reviews Editor at Development, all views here are my own and do not represent the journal.

——

Cambridge, England – 2026
One of April’s many Mondays.

10:00 – Get to work, catch up with the office, grab a coffee. Our core office hours are 10am till 2pm so, as a night owl, I take full advantage of this and start at 10.

10:05 – Check emails that have come in through the weekend, as well as the various reports and notification we automatically receive from our online submissions system. An author who I am really excited about has agreed to write a review for us – whoop! I respond immediately and get stuck into clearing my inbox, which seems to be perennially full no matter how much I try to empty it.

10:30 – Soreen* break! Have a quick chat with the lovely preLights Community Manager, Reinier, about possible exciting preprints to highlight between bites of sticky sugary goodness. I promise to also upload the preprints list I collated at BSDB a few weeks ago. But for now, back to emails.

11:30 – Just received a message from Saanjbati about moving an article around between scheduled issues, so we have a quick chat about this. Then back to working through my inbox.

12:30 – Done with emails! Whew. A few commissioning emails have been sent out, feedback on synopsis given, reviewers chased, submission deadlines updated. Deep dives into synthetic biology and photoreceptors surfaced from. Just a little bit more admin to go…

12:45 – … and we’re done! Time for lunch outside in some suprisingly good weather.

13:15 – After a little bit of sun (and a touch of wind), it’s back to the desk for me. This afternoon, I have a slightly overdue meeting report to send round. It’s been delayed because I lost all the notes I’d been taking on it when my computer decided to restart itself whilst I was at the conference dinner and disco. Obviously a massive shame, but it also pushes me to ruminate harder over my notes in an attempt to rescue them, which might lead me to find other ideas…? Or at least that’s what I tell myself.

13:30 – I’ve realised I need to book my hotel for a trip later this year, which I’ve already forgotten to do four times. I get this out of the way first before I forget a fifth. Makes me excited for EuroEvoDevo in Glasgow!

14:30 – Somehow got sidetracked into looking up biorxiv references made during BSDB, which prompted another deep dive into recent preprints published under ‘Developmental Biology’. Spend a few minutes in awe of how quickly research is moving in certain directions and the seemingly masses of interest in biophysical/quantitative biology. Send some ramblings to Reinier and feel mildly envious of his job.

14:40 – Back to work on my core responsibilities! I’m only halfway through thinking about the meeting report and doing follow-ups on it. But it’s already time to look through the list of articles that were accepted in the past week and think about which should be highlighted.

14:58 – Done, just in time for the research highlight (RH) meeting! This is our weekly meeting where we discuss all the back section (i.e. primary research) articles and decide which to highlight. There are so many interesting papers this week, it’ll be quite hard to choose and I’m excited for the discussion.

15:35 – RHs have been picked, and I’m back at my desk to do a final read-through of the paper I’ve been assigned, just to make sure it’s as interesting as it seemed from my initial read-through.

15:40 – An email from our production team has just come through about a review article I’ve handled. Spend 10 minutes on this. Then another message comes through regarding some travel admin. Another 10 minutes gone.

16:00 – Back to reading this week’s RH paper. I personally really like it and am even more excited to write the highlight, although some of it is feeling quite anatomically complicated and will be difficult to describe in just 200 words without an illustration… Ohh well, that’s a problem for the future.

16:15 – OK, the various miscellaneous items that came in are done. Time to really lock-in on the meeting report, which needs to be sent today. It’s a nice creative exercise to reflect on all the science discussed at the meeting, but quite stressful to go through the whole programme and all my notes and synthesise something coherent for the rest of the team when under a time pressure.

16:50 – My brain is fried, my fingers feel like they’re about to fall off and my spirits are in dire need of chocolate. But the meeting report has finally been sent off! Time to wrap up a few things. As always, some important emails trickle through right as I’m about to leave. I resolve to address them on my way home.

17:00 – I leave early on Mondays so rush out for my bus. Bye!

*(other brands of malt loaves exist etc. etc.)

——

Did you know that anyone can publish on the Node? If you’ve been inspired to write a piece for the developmental biology community, feel free to register an account and then make your own blog post here: https://thenode.biologists.com/wp-admin/post-new.php

The post A day in the life of a Reviews Editor (at Development) appeared first on the Node.

Preprint highlights

• Canonical mTOR signaling supports complete fin regeneration selected by Theodora M Stougiannou
• Whole-Cell Proteomics Identifies Novel Regulators of Ciliogenesis Beyond the Axoneme selected by Jawdat Sandakly
• Synthetic lumen rounding directs neural progenitor division mode selected by Sristilekha Nath
• Lamin A/C directs nucleosome-scale chromatin remodeling to define early lineage segregation in mammals selected by Deevitha Balasubramanian
• Abnormal ventricular wall patterning precedes and drives MYBPC3 hypertrophic cardiomyopathy selected by Theodora M Stougiannou

Preprint:

Theodora M Stougiannou

Preprint:

Canonical mTOR signaling supports complete fin regeneration
Josane F. de Sousa, Gabriela Lima, Louise Perez, Michaela Tsanova, Cyrus Bronson, Garrison Boehl, Icyss Sargeant, Rogerio Gomes, Aline C. Dragalzew, Wainna B. Mendes, Igor Schneider

preLight:

Fins, and cells, and signals, and regeneration, oh my! How the Senegal bichir regrows its fins after amputation.

The authors of this preprint investigate fin regeneration in the Senegal bichir (Polypterus senegalus), a type of ray-finned fish capable of full fin regeneration; this biological characteristic is quite impressive on its own, as the fin includes different tissues, such as skeletal, cartilaginous, muscular and connective tissue with complexity comparable to that found in tetrapod limbs.

The preprint authors show that regeneration entails the activation of canonical mTOR cellular programs, as treatment with the mTOR inhibitor rapamycin prevented this regeneration, though wound healing proceeded normally. Signaling was activated upon amputation, first in epithelial cells in the epidermis and then in adjacent mesenchymal cells below the superficial layers, as well as myeloid cell types.

It seems that mTOR programs in myeloid populations are responsible for the coordination of regenerative procedures across different cell types, as well as its eventual resolution.  Moreover, existence of such programs in species of fish highlights the ‘ancient’ evolutionary origins of tissue regeneration, giving hope for application of these principles in other species.

Jawdat Sandakly

Preprint:

Whole-Cell Proteomics Identifies Novel Regulators of Ciliogenesis Beyond the Axoneme
Xiaolu Xu, Yanbao Yu, Tony Zheng, Fiona Clark, Jean Ross, Neha Sindhu, Andre L P Tavares, John B Wallingford, Shuo Wei, Jian Sun

preLight:

Uncovering new players in ciliogenesis by whole-cell proteomics

Motile cilia are microtubule-based organelles that are involved in fundamental biological processes such as embryonic development, signalling, and mucus clearance. Their dysfunction results in several disorders known as ciliopathies.

Several efforts over the years have helped in elucidating the molecular architecture of motile cilia and in understanding ciliary structures and functions. Moreover, previous proteomic studies provided valuable insights into the axonemal composition. However, many molecular regulators of ciliogenesis remain unknown and other critical cellular components beyond the axoneme involved in ciliogenesis require further investigation.

In this preprint, the authors performed a high-resolution whole-cell proteomic profile of multiciliated cells (MCC), whose function is regulated by axonemal proteins, basal bodies, cytoplasmic factors, and nuclear components. They induced MCC cell fate in Xenopus, therefore enriching ciliary proteins and generating mucociliary organoids. Following their high-depth proteomic profiling, they identified several previously uncharacterized proteins that are essential for MCC maintenance and ciliogenesis. Through in situ hybridization, immunostaining, and gene knockdown, they further confirmed the new candidates, thus providing new potential targets to be further explored to gain a better understanding of the mechanisms related to ciliopathies.

Sristilekha Nath

Preprint:

Synthetic lumen rounding directs neural progenitor division mode
Marina Marchenko, Guillermo Martínez Ara, Juslina Pulikkal, Keisuke Ishihara, Miki Ebisuya

preLight:

Cells read lumen geometry to instruct division mode and lineage progression

During early brain development, tissue geometry – including lumen geometry – dynamically changes; a process which varies across species. But does this geometry simply result from development, or does it actively instruct how cells behave?

The authors of this preprint investigate this question by artificially controlling lumen geometry in brain organoids using two approaches: chemical induction of Shroom3, a protein that drives apical constriction and OptoShroom3, an optogenetic system enabling precise, light-controlled activation. The latter enables spatially targeted control without affecting overall Shroom3 levels within the organoids.

The results reveal that lumen geometry is not a passive consequence of development, but an active regulator of cell behavior. Chemically-induced Shroom3 organoids formed much rounder lumens and neural buds, and generated basal progenitor cells faster than controls, while cells gradually switched from vertical to horizontal cleavage planes over time, a critical reorientation since horizontal division results in asymmetric cell division that generates more basal progenitors, whereas vertical division (in controls) maintains more apical progenitors. When the authors used OptoShroom3 to create rounded lumens with localized blue light illumination, apical progenitor cells in target buds similarly shifted toward horizontal cleavage planes within an hour, whereas those without illumination (control bud within the same organoid) did not.

These findings demonstrate that cells ‘read’ their geometric environment to make developmental decisions, suggesting lumen shape as a key determinant, not merely a consequence of morphogenetic outcomes, a principle likely applicable broadly across organs and species. 

Deevitha Balasubramanian

Preprint:

Lamin A/C directs nucleosome-scale chromatin remodeling to define early lineage segregation in mammals
Alice Sherrard, Liangwen Zhong, Caroline Hoppe, Srikar Krishna, Scott Youlten, Curtis W. Boswell, Stephen Cross, Fiona E. Sievers, Goli Ardestani, Denny Sakkas, Liyun Miao, Zachary D. Smith, Berna Sozen, Antonio J. Giraldez

preLight:

Nuclear lamins direct the first lineage decision in mammalian cells

The first lineage decision during mammalian development into the inner cell mass (ICM) and trophectoderm (TE) is well known to be initiated by transcriptional and epigenetic factors and reinforced by mechanical forces. While global chromatin organization is understood to be important for this process, it remains unclear how the fine-scale distribution of chromatin and nucleosomes plays a role in these cell fate decisions.

To investigate this, the authors set up an improved chromatin electron tomography protocol called ChromEMT to observe nanometer-scale sub-nucleosomal structures. Using ChromEMT on human and mouse cell cultures before, during, and after specification into ICM and TE, they identified key differences in chromatin packing density and nucleosome spacing between these lineages. Importantly, they found that TE nuclei have highly compacted chromatin at their nuclear periphery. In line with this increased peripheral compaction, the authors could show that proteins located at the inner nuclear membrane, particularly lamins A and C, are specifically upregulated in TE cells across mammals. Loss of Lamin A/C resulted in loss of peripheral chromatin compaction and upregulation of pluripotency genes in TE cells, suggesting an overall transition to ICM-like characteristics. This, in turn, impairs normal progression through embryogenesis.

In concert with many more supporting findings, this preprint demonstrates how chromatin compaction and nuclear lamins directly shape early mammalian development.

Theodora M Stougiannou

Preprint:

Abnormal ventricular wall patterning precedes and drives MYBPC3 hypertrophic cardiomyopathy
Alejandro Salguero-Jiménez, Alba Pau-Navalón, Marcos Siguero-Álvarez, Carlos Relaño-Rupérez, Javier Santos-Cantador, María Sabater-Molina, Xiaoxi Luo, Laura Lalaguna, Laura Sen-Martín, Daniel Martín Pérez, Abel Galicia Martín, Bin Zhou, Juan Antonio Bernal Rodríguez, Fátima Sánchez-Cabo, Enrique Lara-Pezzi, Jorge Alegre-Cebollada, Juan R. Gimeno-Blanes, Donal MacGrogan, José Luis de la Pompa

preLight:

More ‘heart’, more problems; a natural history of myocardial hypertrophy progression from embryonic development to adulthood and the role of sarcomeric protein mutations (Mybpc3) in its emergence.

The authors of this preprint investigated the developmental biology underlying hypertrophic cardiomyopathy and left ventricular non-compaction in mice. To this end, they used CRISPR-Cas9, a method used to induce genetic alterations, to introduce MYBPC3 frameshift mutations in the mouse genome and then followed these mice from embryonic and fetal development into adulthood. Adult mice with these mutations displayed hypertrophic cardiomyopathy but with no evidence of left ventricular non-compaction, as opposed to humans. These formations began as trabecular enlargement and crypt enlargement during embryonic development and progressed to hypertrophy in adulthood. Lineage tracing studies further showed invasion of cardiomyocytes normally found in compact myocardium (Hey+ cardiomyocytes), into the developing trabeculae, while after birth, Hey+ cardiomyocytes became restricted to compact myocardium and the inner trabecular myocardium underwent hypertrophy. This is associated with downregulation of the Prdm16; this study highlights how the latter has potential to combat myocardial hypertrophy.

This study highlights the natural history of myocardial hypertrophy and how loss of Mybpc3 is associated with reduction in Prdm16 and onset of pathological hypertrophic remodeling.

The post preLighters’ choice – A curated selection of recent preprints appeared first on the Node.

The Medical Research Council provided £750K in funding for the National Mouse Genetics Network’s Business Engagement Fund with a call for applications in early 2023. The Business Engagement Fund supported 3–12-month projects, providing grants of £15–100K, with the expectation that matched funding would be provided by industry collaborators. Funded projects were designed to build and strengthen collaborations between the Network and businesses through feasibility, pilot, or initial studies. These activities aimed to explore ideas and generate initial data to support the development of competitive collaborative grant proposals.

We are now reporting on the first of these projects, highlighting how collaborative endeavours of this kind can help shape preclinical research and accelerate the development of therapeutic interventions.

The project was a partnership between Professor Nick Greene of University College London and OutFox Bio. Nick is a member of the Congenital Anomalies Cluster and a leading academic researcher studying a range of birth defects, with a long-standing interest in the role of folates in development and inherited metabolic disease. OutFox Bio is a delivery technology company focused on the development and optimisation of next-generation lipid nanoparticle (LNP) gene delivery technologies, designed to enable new gene therapy approaches and expand their potential applications.

A life-limiting incurable disease

Non-Ketotic Hyperglycinemia (NKH) is a life-limiting autosomal recessive neurometabolic disease that presents in neonates with lethargy, hypotonia, myoclonic jerks and apnoea. Affected children experience profound neurological impairment and complex epilepsy. Around one-third of infants with severe neonatal-onset NKH die within the first year, but age at death is highly variable, with some children surviving into their teenage years.

NKH is caused by mutations in genes that encode the glycine cleavage system (GCS). Most patients (80%) carry mutations in GLDC (glycine decarboxylase), with the remainder carrying mutations in AMT (aminomethyltransferase). The GCS decarboxylates glycine, with the concomitant transfer of a one-carbon (1C) group to tetrahydrofolate (THF), generating methylene-THF. Subsequent reactions in folate one-carbon metabolism (FOCM) provide 1C groups for multiple outputs, including nucleotide biosynthesis and methylation reactions. Hence, GCS dysfunction leads both to the accumulation of excess glycine in the body and to suppression of FOCM.

There is no cure for NKH; current treatments have limited efficacy. Prognosis remains very poor, highlighting an urgent unmet need for novel therapies. There is currently no established standard of care for NKH, although patients are typically treated with multiple anti-seizure medications. The most common treatment is sodium benzoate, which is administered to lower circulating glycine by stimulating glycine conjugation in the liver, generating hippurate (benzoylglycine) for excretion. Benzoate helps with seizure control but can be toxic and is associated with severe gastrointestinal side effects, necessitating long-term co-administration of proton-pump inhibitors which may carry additional risks. Replacement of benzoate has been highlighted as a priority during discussions with families of affected children.

To investigate NKH pathogenesis and develop novel treatments, Nick’s group developed a GLDC-deficient mouse model that recapitulates hallmark features of the disease, including elevated plasma and tissue glycine and neurological abnormalities. Loss of glycine cleavage system activity was confirmed by enzymatic assay and metabolic tracing using isotopically labelled glycine. Glycine is both a biomarker and a therapeutic target in NKH; both glycine and guanidinoacetate, a glycine–arginine conjugate, are epileptogenic.

In GLDC-deficient mice, the group observed that liver-specific reinstatement of GLDC expression or stimulation of hepatic glycine conjugation through benzoate administration led to normalisation of liver tissue glycine and glycine derivatives, correction of blood glycine concentrations, and reduction of glycine levels in the brain, the main site of NKH pathogenesis. These studies provide proof of principle for liver-directed therapy as a means of controlling systemic and brain glycine levels.

The causative genes are known, making NKH potentially amenable to therapies that restore gene expression. The aim is to develop RNA-based approaches to reinstate GLDC expression and normalise metabolism in NKH. Lipid nanoparticle (LNP)-mediated delivery of mRNA to the liver represents an attractive methodology for therapeutic gene expression. LNP systems have proven to be effective and safe for mRNA delivery and are already in clinical use for other conditions.

In this NMGN Business Engagement Fund project, undertaken in partnership with OutFox Bio, the team initially sought to address two key questions using a reporter-encoding mRNA.  First, they tested whether the liver in NKH remains amenable to LNP-mediated mRNA delivery despite abnormal metabolism. Second, they sought to identify the optimal LNP composition for mRNA delivery to the liver in the NKH GLDC-deficient mouse model. The team identified LNP compositions with improved efficacy compared with clinically approved benchmarks. They also confirmed that compromised glycine metabolism in the liver does not hinder uptake or expression of LNP-delivered mRNA in the NKH mouse model. For example, expression of LNP-mediated reporter expression was at least as high in GLDC-deficient mice as in wild-type mice following treatment with each LNP composition. These findings provided the proof of concept for extending the project to therapeutic mRNA, prioritising the lead LNPs.

The ongoing objective of the project is to develop an mRNA-based therapy that reinstates liver GLDC expression, normalises metabolism, and improves neurological outcomes in the GLDC-deficient NKH mouse model. Outputs from this project are expected to provide an evidence base for advancing this approach towards clinical trials in children with NKH.

Nick presented some of this work at the NKH Crusaders 11th Annual International Family Conference in Boston, where he gave a presentation and took part in several round-table discussions. The event was reported on social media, where Nick’s talk was also mentioned.

The post A Business Engagement Fund project to test Lipid Nanoparticle (LNP)-mediated mRNA delivery and efficacy appeared first on the Node.

Sure, we do what we do for the maths, physics and molecular biology underlying development and evolution … but also out of fascination for the beauty and complexity of Life.

So, we decided to produce a 3.5-minute movie in which an extraordinary guest celebrates Attenborough’s birthday. 

See for yourself: https://youtu.be/oAwsMkMdAMA 

We hope that our movie will contribute to honouring the man who helped the world fall in love with nature.

Social media links:
-X/Twitter: https://x.com/LANEVOL/status/2052621779261124672?s=20
-Bluesky: https://bsky.app/profile/lanevol.bsky.social/post/3mlcysxxa3s25
-LinkedIn: https://www.linkedin.com/

Have a great Attenborough weekend !

Michel & Athanasia
https://www.lanevol.org/news/article/extraordinary-guest-celebrate-sir-david-attenborough
https://www.lanevol.org

The post An extraordinary guest to celebrate Sir David Attenborough appeared first on the Node.

Location: Buxted Park, East Sussex, UK

Organisers: Elena Casacuberta and James Gahan

One of the central questions in developmental biology is how different cell fates are generated from a single founding cell. Although great strides have been made in our understanding of this problem in animals, the evolutionary origins of this process are not understood. It is known that many unicellular organisms progress through different cell stages during their life cycle, known as temporal cell differentiation, and it has been hypothesized that spatial cell differentiation (as seen in animals) evolved from this more ancient differentiation-mode. A full understanding of how this occurred has been hampered by a lack of information on the basic principles underlying temporal cell differentiation in the closest relatives of animals, the unicellular holozoans.

In recent years, several studies have revealed that many of the genes and pathways directly related to development and cell fate in animals were already present in their unicellular ancestors. Moreover, many examples have shown the formation of specialized cell types in response to specific environmental ques and transient multicellular structures have been reported in many unicellular holozoan lineages. Therefore, recent discoveries strongly point towards an earlier origin of several developmental processes, including cell differentiation, than was previously thought and make a strong case that understanding the mechanisms underpinning “development” in these unicellular lineages will be key to understand the emergence of definitive animal cell differentiation and development.

The Workshop will consist of sessions of talks and discussions centred around various aspects of development to unicellular holozoans. Each session will contain a mixture of researchers working on unicellular holozoans and those working on other eukaryotic systems who will provide alternative insights. Through these sessions the Workshop will build knowledge aiming to produce a white-paper document outlining the emerging conceptual framework in the field, the major outstanding questions as well as seeding collaborative efforts to address these questions.

Organisers & speakers

Elena Casacuberta Institute for Evolutionary Biology, Spain
James Gahan University of Galway, Ireland

Detlev Arendt EMBL, Germany
David Booth University of California, San Francisco, USA
Thibaut Brunet Institut Pasteur, France
Pawel Burkhardt University of Bergen, Norway
Susana Coelho Max Planck Institute for Biology Tübingen, Germany
Omaya Dudin University of Geneva, Switzerland
Nicole King University of California, Berkeley, USA
Lucie Laplane CNRS, Université Paris, France
Eric Libby Umeå University, Sweden
Aurora Mihaela Nedelcu University of New Brunswick, Canada
Àlex de Mendoza Queen Mary University of London, United Kingdom
Iñaki Ruiz-Trillo The Institute of Evolutionary Biology, Spain
Florentine Rutaganira Stanford University, USA
Arnau Sebé-Padrós Centre for Genomic Regulation, Spain
Hiroshi Suga Prefectural University of Hiroshima, Japan
Katrina Velle UMass Dartford, USA
Renske Vroomans University of Cambridge, United Kingdom

We offer 10 funded places for early-career researchers (PhD, postdocs and PIs in the first three years of their first appointment) to attend our Workshops along with the 20 invited speakers. We just ask that you pay for your own travel costs. If you would like to attend please complete the online application form and include a one page CV and a letter of support from your supervisor. If your supervisor would prefer to send the letter directly to us please ask them to email it to workshops@biologists.com

All attendees are expected to actively contribute to the Workshops by asking questions at presentation sessions and taking part in discussions, as well as giving a short talk on their research.

The early-career research deadline is on Friday 12 June 2026. For more information, visit the Company’s Workshops page.

The post Rethinking Cell Differentiation and Development: A Unicellular Perspective Workshop appeared first on the Node.