Please vote for your favourite image using the poll at the bottom of the page. The voting will close on Wednesday, 24 June 2026, at 13:00 BST (UTC + 1). One vote per person.

Browse through the gallery (click to view full image)

The post Vote for a Development cover from the Quintay International Course on Developmental Biology appeared first on the Node.

In this quarterly webinar series, hosted by Pablo J. Sáez and Valeria Venturini, we aim to showcase the latest research in the field, and we’d love to hear from researchers (especially early-career researchers) who would like to present their work. You can get in touch with us at focalplane@biologists.com or fill in our short application form.

The post New FocalPlane features… webinar series on cell migration appeared first on the Node.

Development, host of the Node, invites you to submit your latest research to our upcoming Special Issue – Plant and Algae Development. This issue will be coordinated by Academic Editor Dominique Bergmann (Stanford University, USA) and Guest Editor Susana Coelho (Max Planck Institute for Biology, Tübingen, Germany).

Developmental biology aims to understand how a single cell, the zygote, becomes a complex multicellular organism with specialised cell types organised into functional units. Derived from unicellular ancestors, algae, plants and animals use shared developmental principles, such as biophysical interactions, signalling, patterning and cell fate determination, to innovate and overcome obstacles for multicellular development, such as coordinating growth. The highly plastic development of photosynthetic organisms have long offered specific opportunities to answer questions regarding the origins of multicellularity, stem cell maintenance and regeneration, as well as environmental and microbial interactions and integration. In recent years, advances in genomics, imaging and synthetic biology are revealing how gene regulatory networks, mechanical biology and signalling interact to shape form and function in plants and algae. Furthermore, mathematical, computational and modelling approaches combined with traditional experimental biology have revealed, explained and predicted quantifiable properties behind phenotypes and across scales. We also see an exciting range of research organisms in use today, including species of algae, moss, ferns, flowering plants and others. In this special issue, we aim to showcase the contribution of plants and multicellular algae to our understanding of development, regeneration and evolution, highlighting quality research across the entire breadth of developmental biology.

The deadline for submitting research papers is 2 November 2026.

The post Call for papers – Plant and Algae Development appeared first on the Node.

This question has followed me for more than a decade. Not as a single project, but as a thread that kept resurfacing—each time forcing us to rethink what we thought we understood.

The idea of comparing regenerative zebrafish with non-regenerative medaka started as one of several proposals I discussed with Didier Stainier when I joined his lab in 2014. At the time, it felt simple: let biology provide the contrast, instead of trying to extract answers from a single system.

Zebrafish regenerate their hearts. Medaka do not. That difference was too striking to ignore (1).

I was in my second postdoc then, aware that I needed something I could carry forward. Didier encouraged me to pursue it, even though it was clearly high-risk. Looking back, that decision shaped everything that followed.

When the data pointed to something unexpected

Together with Rubén Marín-Juez —who later became both a key collaborator and a close friend—we established a cryoinjury model in medaka and generated our first comparative RNA-seq datasets (2).

What we saw was not what we expected.

Instead of cardiomyocyte-centered differences, which at the time were widely viewed as the primary drivers of cardiac regeneration, the strongest signals pointed toward immune responses and angiogenesis (2). I remember hesitating. It felt like we were drifting away from what many would consider the “core” of cardiac regeneration.

But the data were clear, and we decided to follow it.

We used to joke that we were like the Maze Runners—moving forward without knowing what was coming next, or how things would end. Rubén focused on angiogenesis and uncovered how revascularization is an early and essential step in regeneration (3, 4). In parallel, I moved toward immunity—despite being warned, quite accurately, that “immunity is too complicated and messy to work with.”

In hindsight, that hesitation reflects something broader. Work in non-mammalian models is often judged by how directly it translates to human biology, rather than by the clarity of the biological principles it can reveal. Yet it is precisely these systems—and the people willing to pursue them—that allow us to uncover mechanisms that are otherwise difficult to see.

A result we thought we understood—but didn’t

What stood out early was that macrophage infiltration appeared delayed and reduced in medaka (2). So, we asked a simple question: what happens if we delay macrophage recruitment in zebrafish?

Using clodrosome, we transiently depleted macrophages prior to injury and observed impaired regeneration.

At the time, we interpreted this primarily as a timing effect—an early delay that irreversibly disrupts regeneration.

This seemed to fit the data. Macrophages eventually came back, and their numbers recovered within about a week. Yet the heart still failed to regenerate.

We moved forward with that explanation.

But it never fully made sense.

Revisiting the problem with better resolution

It was only years later, after I started my lab at the Institute of Biomedical Sciences at Academia Sinica in Taiwan, that we revisited this question with better tools.

Through temporal single-cell profiling—driven in large part by the careful and persistent work of Ke-Hsuan Wei, one of the first PhD students in my lab—we realized something we had completely missed before: clodrosome was not simply delaying macrophages—it was preferentially depleting the resident macrophage population (5).

These cardiac resident-like macrophage subsets turned out to be essential for heart regeneration—coordinating revascularization, cardiomyocyte survival, debris clearance, and extracellular matrix remodeling.

That realization reframed everything.

Even when we allowed extended recovery, giving circulating, monocyte-derived macrophages ample time to repopulate the heart, regeneration did not recover.

At that moment, the entire story finally made sense.

It was not only about timing. It was also about identity.

What initially appeared to be a delay in macrophage function was, in fact, the loss of a specific and irreplaceable cell population.

Turning a non-regenerative system on

We then asked the opposite question: instead of suppressing the immune response, could activating it change the outcome?

Poly I:C—identified through comparative transcriptomics—enhanced macrophage recruitment and, unexpectedly, enabled de novo regeneration in medaka (2).

That was one of those moments when you don’t immediately trust the data. We repeated the experiments, trying to convince ourselves it wasn’t an artifact.

But it held.

Regenerative capacity began to look less like a fixed property and more like something that could be modulated.

From immune identity to regenerative signal

Our recent paper in PNAS represents the latest step in this progression (6).

Led by Kaushik Chowdhury, this phase of the work brought together comparative analysis, single-cell profiling, and functional experiments to identify a regeneration-associated macrophage population induced by poly I:C.

These macrophages localize to the injury border zone and express Granulin.

What started as a candidate marker became a functional insight. Through a series of carefully executed experiments, the team showed that recombinant Granulin alone is sufficient to promote cardiomyocyte proliferation and reduce scarring—linking immune activation to a concrete regenerative outcome.

Closing the loop, granulin expression is also activated in zebrafish following cardiac injury and is essential for heart regeneration.

Basic science, unexpected translation

I always consider myself a basic scientist and a developmental biologist. None of this work started with a translational goal.

It was driven by curiosity—by a question that seemed fundamental, but not immediately “useful” or “applicable”.

And yet, it led us to a concept that is inherently translational: that regeneration might be induced through immune modulation.

As Didier once put it:

“One never really knows when a basic science finding will transform translational research… CRISPR/Cas9 is just one recent example.”(7)

That perspective has stayed with me throughout this journey. Especially at moments when the work felt uncertain, or when its relevance was not immediately obvious.

Still the same question

If there is one idea that has gradually emerged, it is that regenerative capacity is not fixed.

It is governed, at least in part, by the identity and function of immune cells—and therefore potentially modifiable.

That doesn’t make the problem simple. But it reframes it.

And in many ways, we are still following the same question.

Just with a clearer understanding of what actually matters—and with a team that made it possible to see it.

References

1.     Ito K, Morioka M, Kimura S, Tasaki M, Inohaya K, Kudo A. Differential reparative phenotypes between zebrafish and medaka after cardiac injury. Developmental Dynamics. 2014;243(9):1106-15.

2.     Lai S-L, Marín-Juez R, Moura PL, Kuenne C, Lai JKH, Tsedeke AT, et al. Reciprocal analyses in zebrafish and medaka reveal that harnessing the immune response promotes cardiac regeneration. eLife. 2017;6:e25605.

3.     Marin-Juez R, Marass M, Gauvrit S, Rossi A, Lai S-L, Materna SC, et al. Fast revascularization of the injured area is essential to support zebrafish heart regeneration. Proceedings of the National Academy of Sciences. 2016;113(40):11237-42.

4.     Marín-Juez R, El-Sammak H, Helker CSM, Kamezaki A, Mullapuli ST, Bibli S-I, et al. Coronary Revascularization During Heart Regeneration Is Regulated by Epicardial and Endocardial Cues and Forms a Scaffold for Cardiomyocyte Repopulation. Developmental Cell. 2019;51(4):503-15.e4.

5.     Wei K-H, Lin IT, Chowdhury K, Lim KL, Liu K-T, Ko T-M, et al. Comparative single-cell profiling reveals distinct cardiac resident macrophages essential for zebrafish heart regeneration. eLife. 2023;12:e84679.

6.     Chowdhury K, Huang C-L, Lin IT, Hung Y-J, Lim KL, Liu H-W, et al. Immune modulation promotes heart regeneration through macrophage and Granulin functions in medaka. Proceedings of the National Academy of Sciences. 2026;123(16):e2524705123.

7.     Grewal S. An interview with Didier Stainier. Development. 2015;142(17):2861-3.

The post From comparison to mechanism: decoding heart regeneration appeared first on the Node.

Normally, at The Company of Biologists (Development’s publisher), we follow a hybrid working model, which means that we work from home for half of the week (which is usually 2-3 days a week for me) and the rest in the office. If you haven’t seen a photo of our office building yet, it is a rather beautiful building, combining the charm of a cottage-style exterior (complete with hipped roofs and classic sash windows) with a bright, modern and open-style office inside.

At Development, Ingrid Tsang and I are the Reviews Editors and we mainly handle the journal’s front-section content (so, that includes Reviews, Spotlights, Perspectives, Hypotheses, Primers, interviews – yeah, we have an extensive list!) and we work closely with Alex Eve, Executive Editor of Development.

I normally start my workday between 9:30-10 am, and my first task is always to reply to emails while sipping my morning coffee (a strong flat white if I am home and a long cappuccino when in the office).

After the first half an hour to attending to emails regarding submissions, chasing authors for their submissions, or finishing off a pending task from the previous day (which often involves taking a final read through a decision letter), I move on to the main tasks of the day.

If I am working on a chunky edit – meaning a developmental edit of a Review article – I would usually block off an entire day for it (at least 7–8 hours). This typically happens once a Review-type article (which we commission in-house and invite authors to submit) has gone through peer review. At that stage, we, the in-house Reviews Editors, read through the full manuscript in detail, commenting on scientific accuracy, language and structure, conciseness and accessibility, journal style, article length and references – all while helping authors address the Reviewers’ comments more effectively. I also go through the figures and legends (we take our display items very seriously, as a single figure often speaks a thousand words), commenting on visual appeal, labelling and other finer details. Developmentally editing an article is usually the most rigorous part of the job, at least in my opinion, as it ensures that the final piece is not only of high quality but also forward-looking and engaging for our wide readership. At Development, we pride ourselves on being extremely hands-on when guiding authors and helping them address both our feedback and the Reviewers’ comments, in order to publish the best possible version of their review.

Another crucial part of our job is commissioning. We have our in-house commissioning meetings every two weeks. So, if you catch me the afternoon before, I am usually frantically reading articles on a certain topic of interest, trying to prepare somewhat cohesive pitches to discuss with the rest of the team. We mainly invite authors to write peer-reviewed, review-type content for us. To identify emerging topics in the field, we attend important conferences, chat with researchers across a wide range of developmental biology disciplines, keep an eye on their websites, analyse research trends across primary research articles and participate in extensive 1-1.5-hour long commissioning meetings.

Once we have agreed on a topic and a suitable author, we invite them to write for us. If they accept our invitation, the author will then often involve their students and collaborators as co-authors, and at that point we discuss the potential scope and type of the article. Of course, we also have a thorough in-house pipeline that monitors the status of all articles from invitation through to acceptance.

When we’re in the office, Alex, Ingrid, Andrea (Community Manager of the Node) and I often chat about various aspects of the job throughout the day – both formally and informally – because our work requires teamwork and collaboration. So, Ingrid and I will often discuss scheduling to make sure our publication pipeline stays tight (i.e. that every Issue publishes a few front-section content). If we have just returned from a conference, we chat with Alex and Andrea about emerging research trends and potential blogs/ posts for the community site. We also bounce around ideas for commissioning topics and share feedback on each other’s pitches, amidst a healthy dose of random life chats.

For me, the day usually ends with a quick catch-up on plans for the next day. This is also when I respond to any remaining email replies from the morning. I usually like to do a final run-through of my to-do list, ticking things off and marking any pending tasks (if there are any). And with that, I sign off for the day!

PS: Ingrid has also written a piece on a day in the life of a Reviews Editor – so do give that a read as well!

The post What does a Reviews Editor do? appeared first on the Node.

Preprint highlights

• Canonical mTOR signaling supports complete fin regeneration selected by Theodora M Stougiannou
• Whole-Cell Proteomics Identifies Novel Regulators of Ciliogenesis Beyond the Axoneme selected by Jawdat Sandakly
• Synthetic lumen rounding directs neural progenitor division mode selected by Sristilekha Nath
• Lamin A/C directs nucleosome-scale chromatin remodeling to define early lineage segregation in mammals selected by Deevitha Balasubramanian
• Abnormal ventricular wall patterning precedes and drives MYBPC3 hypertrophic cardiomyopathy selected by Theodora M Stougiannou

Preprint:

Theodora M Stougiannou

Preprint:

Canonical mTOR signaling supports complete fin regeneration
Josane F. de Sousa, Gabriela Lima, Louise Perez, Michaela Tsanova, Cyrus Bronson, Garrison Boehl, Icyss Sargeant, Rogerio Gomes, Aline C. Dragalzew, Wainna B. Mendes, Igor Schneider

preLight:

Fins, and cells, and signals, and regeneration, oh my! How the Senegal bichir regrows its fins after amputation.

The authors of this preprint investigate fin regeneration in the Senegal bichir (Polypterus senegalus), a type of ray-finned fish capable of full fin regeneration; this biological characteristic is quite impressive on its own, as the fin includes different tissues, such as skeletal, cartilaginous, muscular and connective tissue with complexity comparable to that found in tetrapod limbs.

The preprint authors show that regeneration entails the activation of canonical mTOR cellular programs, as treatment with the mTOR inhibitor rapamycin prevented this regeneration, though wound healing proceeded normally. Signaling was activated upon amputation, first in epithelial cells in the epidermis and then in adjacent mesenchymal cells below the superficial layers, as well as myeloid cell types.

It seems that mTOR programs in myeloid populations are responsible for the coordination of regenerative procedures across different cell types, as well as its eventual resolution.  Moreover, existence of such programs in species of fish highlights the ‘ancient’ evolutionary origins of tissue regeneration, giving hope for application of these principles in other species.

Jawdat Sandakly

Preprint:

Whole-Cell Proteomics Identifies Novel Regulators of Ciliogenesis Beyond the Axoneme
Xiaolu Xu, Yanbao Yu, Tony Zheng, Fiona Clark, Jean Ross, Neha Sindhu, Andre L P Tavares, John B Wallingford, Shuo Wei, Jian Sun

preLight:

Uncovering new players in ciliogenesis by whole-cell proteomics

Motile cilia are microtubule-based organelles that are involved in fundamental biological processes such as embryonic development, signalling, and mucus clearance. Their dysfunction results in several disorders known as ciliopathies.

Several efforts over the years have helped in elucidating the molecular architecture of motile cilia and in understanding ciliary structures and functions. Moreover, previous proteomic studies provided valuable insights into the axonemal composition. However, many molecular regulators of ciliogenesis remain unknown and other critical cellular components beyond the axoneme involved in ciliogenesis require further investigation.

In this preprint, the authors performed a high-resolution whole-cell proteomic profile of multiciliated cells (MCC), whose function is regulated by axonemal proteins, basal bodies, cytoplasmic factors, and nuclear components. They induced MCC cell fate in Xenopus, therefore enriching ciliary proteins and generating mucociliary organoids. Following their high-depth proteomic profiling, they identified several previously uncharacterized proteins that are essential for MCC maintenance and ciliogenesis. Through in situ hybridization, immunostaining, and gene knockdown, they further confirmed the new candidates, thus providing new potential targets to be further explored to gain a better understanding of the mechanisms related to ciliopathies.

Sristilekha Nath

Preprint:

Synthetic lumen rounding directs neural progenitor division mode
Marina Marchenko, Guillermo Martínez Ara, Juslina Pulikkal, Keisuke Ishihara, Miki Ebisuya

preLight:

Cells read lumen geometry to instruct division mode and lineage progression

During early brain development, tissue geometry – including lumen geometry – dynamically changes; a process which varies across species. But does this geometry simply result from development, or does it actively instruct how cells behave?

The authors of this preprint investigate this question by artificially controlling lumen geometry in brain organoids using two approaches: chemical induction of Shroom3, a protein that drives apical constriction and OptoShroom3, an optogenetic system enabling precise, light-controlled activation. The latter enables spatially targeted control without affecting overall Shroom3 levels within the organoids.

The results reveal that lumen geometry is not a passive consequence of development, but an active regulator of cell behavior. Chemically-induced Shroom3 organoids formed much rounder lumens and neural buds, and generated basal progenitor cells faster than controls, while cells gradually switched from vertical to horizontal cleavage planes over time, a critical reorientation since horizontal division results in asymmetric cell division that generates more basal progenitors, whereas vertical division (in controls) maintains more apical progenitors. When the authors used OptoShroom3 to create rounded lumens with localized blue light illumination, apical progenitor cells in target buds similarly shifted toward horizontal cleavage planes within an hour, whereas those without illumination (control bud within the same organoid) did not.

These findings demonstrate that cells ‘read’ their geometric environment to make developmental decisions, suggesting lumen shape as a key determinant, not merely a consequence of morphogenetic outcomes, a principle likely applicable broadly across organs and species. 

Deevitha Balasubramanian

Preprint:

Lamin A/C directs nucleosome-scale chromatin remodeling to define early lineage segregation in mammals
Alice Sherrard, Liangwen Zhong, Caroline Hoppe, Srikar Krishna, Scott Youlten, Curtis W. Boswell, Stephen Cross, Fiona E. Sievers, Goli Ardestani, Denny Sakkas, Liyun Miao, Zachary D. Smith, Berna Sozen, Antonio J. Giraldez

preLight:

Nuclear lamins direct the first lineage decision in mammalian cells

The first lineage decision during mammalian development into the inner cell mass (ICM) and trophectoderm (TE) is well known to be initiated by transcriptional and epigenetic factors and reinforced by mechanical forces. While global chromatin organization is understood to be important for this process, it remains unclear how the fine-scale distribution of chromatin and nucleosomes plays a role in these cell fate decisions.

To investigate this, the authors set up an improved chromatin electron tomography protocol called ChromEMT to observe nanometer-scale sub-nucleosomal structures. Using ChromEMT on human and mouse cell cultures before, during, and after specification into ICM and TE, they identified key differences in chromatin packing density and nucleosome spacing between these lineages. Importantly, they found that TE nuclei have highly compacted chromatin at their nuclear periphery. In line with this increased peripheral compaction, the authors could show that proteins located at the inner nuclear membrane, particularly lamins A and C, are specifically upregulated in TE cells across mammals. Loss of Lamin A/C resulted in loss of peripheral chromatin compaction and upregulation of pluripotency genes in TE cells, suggesting an overall transition to ICM-like characteristics. This, in turn, impairs normal progression through embryogenesis.

In concert with many more supporting findings, this preprint demonstrates how chromatin compaction and nuclear lamins directly shape early mammalian development.

Theodora M Stougiannou

Preprint:

Abnormal ventricular wall patterning precedes and drives MYBPC3 hypertrophic cardiomyopathy
Alejandro Salguero-Jiménez, Alba Pau-Navalón, Marcos Siguero-Álvarez, Carlos Relaño-Rupérez, Javier Santos-Cantador, María Sabater-Molina, Xiaoxi Luo, Laura Lalaguna, Laura Sen-Martín, Daniel Martín Pérez, Abel Galicia Martín, Bin Zhou, Juan Antonio Bernal Rodríguez, Fátima Sánchez-Cabo, Enrique Lara-Pezzi, Jorge Alegre-Cebollada, Juan R. Gimeno-Blanes, Donal MacGrogan, José Luis de la Pompa

preLight:

More ‘heart’, more problems; a natural history of myocardial hypertrophy progression from embryonic development to adulthood and the role of sarcomeric protein mutations (Mybpc3) in its emergence.

The authors of this preprint investigated the developmental biology underlying hypertrophic cardiomyopathy and left ventricular non-compaction in mice. To this end, they used CRISPR-Cas9, a method used to induce genetic alterations, to introduce MYBPC3 frameshift mutations in the mouse genome and then followed these mice from embryonic and fetal development into adulthood. Adult mice with these mutations displayed hypertrophic cardiomyopathy but with no evidence of left ventricular non-compaction, as opposed to humans. These formations began as trabecular enlargement and crypt enlargement during embryonic development and progressed to hypertrophy in adulthood. Lineage tracing studies further showed invasion of cardiomyocytes normally found in compact myocardium (Hey+ cardiomyocytes), into the developing trabeculae, while after birth, Hey+ cardiomyocytes became restricted to compact myocardium and the inner trabecular myocardium underwent hypertrophy. This is associated with downregulation of the Prdm16; this study highlights how the latter has potential to combat myocardial hypertrophy.

This study highlights the natural history of myocardial hypertrophy and how loss of Mybpc3 is associated with reduction in Prdm16 and onset of pathological hypertrophic remodeling.

The post preLighters’ choice – A curated selection of recent preprints appeared first on the Node.

Location: Buxted Park, East Sussex, UK

Organisers: Elena Casacuberta and James Gahan

One of the central questions in developmental biology is how different cell fates are generated from a single founding cell. Although great strides have been made in our understanding of this problem in animals, the evolutionary origins of this process are not understood. It is known that many unicellular organisms progress through different cell stages during their life cycle, known as temporal cell differentiation, and it has been hypothesized that spatial cell differentiation (as seen in animals) evolved from this more ancient differentiation-mode. A full understanding of how this occurred has been hampered by a lack of information on the basic principles underlying temporal cell differentiation in the closest relatives of animals, the unicellular holozoans.

In recent years, several studies have revealed that many of the genes and pathways directly related to development and cell fate in animals were already present in their unicellular ancestors. Moreover, many examples have shown the formation of specialized cell types in response to specific environmental ques and transient multicellular structures have been reported in many unicellular holozoan lineages. Therefore, recent discoveries strongly point towards an earlier origin of several developmental processes, including cell differentiation, than was previously thought and make a strong case that understanding the mechanisms underpinning “development” in these unicellular lineages will be key to understand the emergence of definitive animal cell differentiation and development.

The Workshop will consist of sessions of talks and discussions centred around various aspects of development to unicellular holozoans. Each session will contain a mixture of researchers working on unicellular holozoans and those working on other eukaryotic systems who will provide alternative insights. Through these sessions the Workshop will build knowledge aiming to produce a white-paper document outlining the emerging conceptual framework in the field, the major outstanding questions as well as seeding collaborative efforts to address these questions.

Organisers & speakers

Elena Casacuberta Institute for Evolutionary Biology, Spain
James Gahan University of Galway, Ireland

Detlev Arendt EMBL, Germany
David Booth University of California, San Francisco, USA
Thibaut Brunet Institut Pasteur, France
Pawel Burkhardt University of Bergen, Norway
Susana Coelho Max Planck Institute for Biology Tübingen, Germany
Omaya Dudin University of Geneva, Switzerland
Nicole King University of California, Berkeley, USA
Lucie Laplane CNRS, Université Paris, France
Eric Libby Umeå University, Sweden
Aurora Mihaela Nedelcu University of New Brunswick, Canada
Àlex de Mendoza Queen Mary University of London, United Kingdom
Iñaki Ruiz-Trillo The Institute of Evolutionary Biology, Spain
Florentine Rutaganira Stanford University, USA
Arnau Sebé-Padrós Centre for Genomic Regulation, Spain
Hiroshi Suga Prefectural University of Hiroshima, Japan
Katrina Velle UMass Dartford, USA
Renske Vroomans University of Cambridge, United Kingdom

We offer 10 funded places for early-career researchers (PhD, postdocs and PIs in the first three years of their first appointment) to attend our Workshops along with the 20 invited speakers. We just ask that you pay for your own travel costs. If you would like to attend please complete the online application form and include a one page CV and a letter of support from your supervisor. If your supervisor would prefer to send the letter directly to us please ask them to email it to workshops@biologists.com

All attendees are expected to actively contribute to the Workshops by asking questions at presentation sessions and taking part in discussions, as well as giving a short talk on their research.

The early-career research deadline is on Friday 12 June 2026. For more information, visit the Company’s Workshops page.

The post Rethinking Cell Differentiation and Development: A Unicellular Perspective Workshop appeared first on the Node.

Both the format and the individual chapters have been updated. Indeed, there are eight new chapters in the book. The initial chapters are mostly new and are introductions to the principles of development, evolution, and ecology. These should allow each student, no matter in which discipline they were originally trained, to take part in subsequent discussions. Plasticity and symbiotic relations during development are highlighted in these chapters and especially in the new introductory chapter. If species are united vertically by evolution and horizontally by ecology, developmental biology provides a third axis permeating them both.

      The second portion of the book integrates these concepts to emphasize

            •the organism as an ecosystem (holobiont theory)

            • heredity as the transmission of genes, epigenetic patterns, cultures, and even particular environments

            • evolution through developmental regulatory genes and symbiosis

            • phenotypic plasticity and evolution

            • the origins of complexity.

 The third section of the book concerns the “downsides” of having such entangled systems of development: teratogenesis, endocrine disruptors, and the developmental origins of adult disease. The book ends with an assessment of what eco-evo-devo science can do to alleviate the biodiversity crisis.

 Over 50 years ago, Leigh van Valen wrote,  “A plausible argument could be made that evolution is the control of development by ecology.” This well-illustrated volume provides evidence for that argument.  We think that Eco-Evo-Devo will show undergraduate and graduate students how developmental biology helps form a new evolutionary framework for the origins and maintenance of biodiversity.

An overview, description, table of contents (and incredibly beautiful cover) can be seen at the Oxford University Press website. 

Scott F. Gilbert

The post New evo-devo textbook ‘Eco-Evo-Devo: The Environmental Regulation of Development, Evolution, and Health’ appeared first on the Node.

You can find more information on the webinar series, including how to register for event notifications and to sign up to give a talk, on our dedicated webpage.

Our first webinar is on Thursday 30 April at 15:00 BST and will feature talks from Beatrice Lace and Simon Gilroy. Beatrice will present, ‘Multiplexing and Endogenous Fluorescence Discrimination Using FLIM in Plants’ and Simon will ask, ‘Do plants feel pain? How imaging has brought plant wound signaling into the human timescale’.

The post New FocalPlane features… webinar series on quantitative plant imaging appeared first on the Node.

We are happy to announce the forthcoming workshop entitled “Roadmap for
EvoDevoMec”,
Nov. 2nd – 5th, 2026, Université Paris Cité.

The link with evolutionary history of an organism is key to understand
embryonic development. Much of the focus has been so far on genetic
circuitry. However, it has become increasingly clear that physical
constraints are an essential aspect at the basis of morphogenetic
processes. We have now reached an exciting stage where it becomes possible
to integrate mechanical considerations in our view of how development has
been shaped during evolution: EvoDevMec.

This new approach has recently raised great interest, but biological
questions still need to be reformulated with precision. In addition, due to
its integrative nature, it poses a scientific and technical challenge: What
type of experimental systems could be used? How could we define
experimental proof of concepts? How can theoretical biophysical models help
integrate various questions and approaches?

We aim at discussing these questions in an informal context, i.e. with
chalk talks. The workshop is limited to 30 persons, from PhD students to
senior scientists from various countries. We will welcome biologists using
a breadth of models (from unicellular organisms to animal and plant
models), physicists (theoreticians, numericians, experimentalists), and
mathematicians.

For application and details see : https://evodevmec2026.sciencesconf.org

Looking forward to seeing you in Paris,
François Fagotto, François Graner, Marie Manceau, Dennis Wörthmüller

The post Roadmap for EvoDevMec – Workshop, Nov 2nd-5th 2026, Paris, France appeared first on the Node.

The preprints this month are hosted on bioRxiv – use these links to get to the section you want.

Developmental biology

• Patterning & signalling
• Morphogenesis & mechanics
• Genes & genomes
• Stem cells, regeneration & disease modelling
• Plant development
• Evo-devo

Cell Biology

Modelling

Reviews

Tools & Resources

Research practice & education

Developmental biology

| Patterning & signalling

Lineage domains and cytoskeletal cables organize a cellular square grid in a crustacean
Beatrice L. Steinert, Leo Blondel, Chandrashekar Kuyyamudi, Evangelia Stamataki, Anastasios Pavlopoulos, Cassandra G. Extavour

An optogenetic toolkit for robust activation of FGF, BMP, & Nodal signaling in zebrafish
Leanne E. Iannucci, Velanganni Selvaraj Maria Thomas, William K. Anderson, Micaela R. Murphy, Caitlin E.T. Donahue, Catherine E. Campbell, Matthew T. Monaghan, Allison J. Saul, Katherine W. Rogers

Directed conversion of porcine extended pluripotent stem cells into trophoblast-like stem cells through modulation of conserved TGF-β and ERK signaling pathways
Chi-Hun Park, Young-Hee Jeoung, JiTao Wang, Bhanu P. Telugu

Synthetic germ granules reveal a direct role of Vasa/DDX4 in RNA localization and translational activation
Ruoyu Chen, Henoc Zinga, Jay S. Goodman, Ruth Lehmann

Determinants of the Transition Zone Width of Morphogen Readouts
Jan A. Adelmann, Roman Vetter, José M. Dias, Johan Ericson, Dagmar Iber

Mechanistic tradeoffs between local and long-range signaling activity in natural and synthetic morphogens
Gavin Schlissel, Anders S. Hansen, Pulin Li

Inflammatory IL-1 signaling remodels epidermal stem cell compartments by suppressing Wnt activity
Hung Manh Phung, Ikuto Nishikawa, Nguyen Thi Kim Nguyen, Aiya K. Yesbolatova, Ahmed M. Hegazy, Tomson Kosasih, Jun Aoi, Satoshi Fukushima, Sho Hiroyasu, Hitoshi Takizawa, Aiko Sada

Cajal-Retzius fate specification is disrupted by constitutive activation of β-Catenin in hem progenitors
Amrita Singh, Arpan Parichha, Debarpita Datta, Mallika Chatterjee, Shubha Tole

Kinetic Control of Out-Of-Equilibrium Dynamics in the RhoA Signaling Cascade Shapes Actomyosin Contractility
Serena Prigent Garcia, Étienne Pinard, Camille N. Plancke, Jing Li, Shashi Kumar Suman, Loan Bourdon, Christelle Gally, Taeyoon Kim, François B. Robin

Dorsal/NF-κB exhibits a dorsal-to-ventral mobility gradient in the Drosophila embryo
Hadel Al Asafen, Natalie M. Clark, Etika Goyal, Sadia Siddika Dima, Hung-Yuan Chen, Thomas Jacobsen, Rosangela Sozzani, Gregory T. Reeves

Position Dependent Feedback Drives Scaling and Robustness of Morphogen Gradients
Lewis Scott Mosby, Zena Hadjivasiliou

TGF-β signaling regulates epithelial permeability in Drosophila ovaries by modulating adhesion independent of actomyosin contractility
Harshath Amal, Thea Jacobs, Max Lohrberg, Stefan Luschnig

Reconstructing signaling histories of single cells via perturbation screens and transfer learning
Nicholas T. Hutchins, Miram Meziane, Claire Lu, Maisam Mitalipova, David Fischer, Pulin Li

Direct cell-to-cell transport of Hedgehog morphogen is aided by the diffusible carrier Shifted/DmWif1
Carlos Jiménez-Jiménez, Gustavo Aguilar, Clara Fernández-Pardo, Markus Affolter, Isabel Guerrero

Arginine Kinase 1 regulates energy homeostasis in Drosophila muscle development
Maria Paula Zappia, Anton Westacott, Hannah Cooke, Rhianna Geary, Libby Travers, Lucia de Castro, Oliver Carty, Maxim V Frolov

Weckle is a molecular switch that diverts Toll signalling from innate immunity towards growth by engaging Yki
Maria Dolores Perez-Sanchez, Guiyi Li, Martin Moncrieffe, Francisca Rojo-Cortés, Karina Malinovska, Emily Sample, Myles Maddick, Marta Moreira, Elizabeth Connolly, Anna Parsons, Roberto Feuda, Nick J. Gay, Alicia Hidalgo

Basement membrane mechanics drives patterned response to developmental signalling
Ana Raffaelli, Tom P.J. Wyatt, Claire S. Simon, Léa M.D. Wenger, Kathy K. Niakan, Ewa K. Paluch, Kevin J. Chalut

A single-cell transcriptomic map of the Xenopus mesonephros reveals conserved nephron patterning across vertebrate kidney forms
Mark E. Corkins, Adrian Romero, MaryAnne A. Achieng, Nils O. Lindström, Rachel K. Miller

Sharp cell-type boundaries emerge from temporal coordination between morphogen signals
Ruiqi Li, Yiqun Jiang, Sarah Platt, Tianchi Xin, Ryan Driskell, Kevin A. Peterson, Sarah Van, Hainan Lam, Shagun Lukkad, Eva-LaRue Barber, Chae Ho Lim, M. Mark Taketo, Yuval Kluger, Peggy Myung

Synthetic reconstitution of planar polarity initiation reveals collective migration as a symmetry-breaking cue
Leah A Wallach, Connor D Thomas, Pulin Li

Epithelial-Mesenchymal Wnt Crosstalk Directs Planar Cell Polarity in the Developing Cochlea
Ippei Kishimoto, Abel P. David, Kevin P. Rose, Balasubramanian Narasimhan, Bradley Efron, Sara E. Billings, Erin L. Su, Wuxing Dong, Taha A. Jan, Ronna Hertzano, Alan G. Cheng

Notch-driven fate asymmetry dictates hair cell behavior via a fate-specific kinase
Emily Atlas, Caleb C. Reagor, Brian Frost, Sapna Krishnakumar, A. J. Hudspeth, Adrian Jacobo

A synNotch-based morphogen detection system reveals sFRP2 enhances Wnt3a signaling
Kosuke Mizuno, Satoshi Toda

| Morphogenesis & mechanics

Tenascin N contributes to spinal motor nerve morphogenesis during development
Charles G. Marcucci, Marieke Jones, Coleman Blanton, Sarah Kucenas

A stress-responsive morphogenetic program of the uterine epithelium safeguards the establishment of early pregnancy
Chihiro Ishizawa, Shizu Aikawa, Yamato Fukui, Xueting He, Ryoko Shimizu-Hirota, Daiki Hiratsuka, Mitsunori Matsuo, Takehiro Hiraoka, Yasushi Hirota

Cell-intrinsic compliance mechanism enables release of tensile stress to prevent tissue rupture
Chun Wai Kwan, Shunta Sakaguchi, Michiko Takeda, Takefumi Kondo, Yu-Chiun Wang

The mechanosensitive protein Zyxin influences Hippo signalling and tissue growth via adherens junctions and basal spot junctions in Drosophila
Harmanjeet Singh, Elliot Brooks, Kyoko Jinnai, Shu Kondo, Samuel A. Manning, Benjamin Kroeger, Kieran F. Harvey

Inverted Assembly of the Lens Within Ocular Organoids Reveals Alternate Paths to Ocular Morphogenesis
Elin Stahl, Miguel Angel Delgado-Toscano, Ishwariya Saravanan, Anastasija Paneva, Joachim Wittbrodt, Lucie Zilova

Long-Range Coupling of Posterior Cell Addition and Anterior Vacuolation Provides Robustness in Notochord Elongation
Carlos Camacho-Macorra, Alberto Ceccarelli, Dillan Saunders, Guillermo Serrano Nájera, Osvaldo Chara, Benjamin Steventon

Pre-cuticle DPY-6 acts as a blueprint for aECM periodic organization in C. elegans
Sophie Mazzoli, Thomas Sonntag, Emma Cadena, Claire Valotteau, Susanna K. Birnbaum, Meera V. Sundaram, Nathalie Pujol

Unified Transcriptome and Mechanics Map of the Intact Mammalian Preimplantation Embryo In Situ
Ehsan Habibi, Anubhav Sinha, Haiqian Yang, Payman Yadollahpour, Yiwei Li, Lani Lee, David A. Wollensak, Zachary D. Chiang, Denny Sakkas, Edward S. Boyden, Ming Guo, Aviv Regev, Fei Chen

A single-cell transcriptomic atlas of inner ear morphogenesis in zebrafish
Akankshi Munjal, Kalki Kukreja, Samara Williams, Toru Kawanishi, Natasha M. O’Brown, Kana Ishimatsu, Allon Klein, Sean G. Tsung-Megason, Ian A. Swinburne

Kindlin-2-Moesin interaction orchestrates sprouting angiogenesis via modulating endothelial membrane mechanics and VEGF signaling
Lu Wang, Yuxin Fu, Zeyang Yu, Yi Lei, Tianjing Yang, Jiayu Liu, Nina Ma, Yuming Liu, Kunfu Ouyang, Kai Zhang, Junhao Hu, Xi Fang, Ying Shen, Jing Zhou, Xiaohong Wang

Tissue-scale mechanics controls differentiation strategy and dynamics of epithelial multilayering
Clémentine Villeneuve, Somiealo Azote Epse Hassikpezi, Marga Albu, Matthias Rübsam, Leah C. Biggs, Sabrina Vinzens, Kai Kruse, Anubhav Prakash, Peter Zentis, Elizabeth Lawson-Keister, Gautier Follain, Johanna Ivaska, Carien M. Niessen, M. Lisa Manning, Sara A. Wickström

Mechanical memory of confinement pressure governs expansion size in epithelial monolayers
Linn Engström, Simon K Schnyder, Johannes K Ahnlide, Valeriia Grudtsyna, Martijn Gloerich, Pontus Nordenfelt, Amin Doostmohammadi, Vinay Swaminathan

| Genes & genomes

Light-entrained chromatin priming poises rapid metamorphosis in a marine sponge
Huifang Yuan, Oceane Blard, Zac Pujic, Bernard M. Degnan, Sandie M. Degnan

Single-cell multiomics identifies key nodes and cis-regulatory elements of the networks specifying the eye domains in zebrafish
Javier Macho Rendón, Rocío Polvillo, Álvaro Gónzalez-Cid, Jorge Corbacho, Silvia Naranjo, Sofia Manzo, Ana Sousa-Ortega, Ana Fernández-Miñán, Juan Tena, Juan Ramón Martínez-Morales

Reciprocal zebrafish-medaka hybrids reveal maternal control of zygotic genome activation timing
Krista R. Briedis-Gert, Gunnar Schulze, Maria Novatchkova, Karin Panser, Luis Enrique Cabrera Quio, Anja Koller, Yixuan Guo, Bradley R. Cairns, Eivind Valen, Andrea Pauli

A Multi-tissue Transcriptomic-Metabolomic Map Linking Maternal High-Fiber Diet to Reduced Offspring Type 2 Diabetes
Tetsuto Katsura, Oluwagbotemi Omojola, Antwi-Boasiasko Oteng, Peng Jiang, Katherine A. Overmyer, Josh Coon, Amadou Gaye, Huishi Toh

A transcriptional code controlling fluid shear stress-induced gene expression
Lucija Fleisinger, Susann Bruche, Hyewon Lim, Anna Rataj, Helena Rodriguez-Caro, Amaury Genovese, Vinesh Vinayachandran, Svanhild Nornes, Dorota Szumska, Dhruv S Gupta, Indrika Ratnayaka, Kira Chouliaras, Marek Giers, Simon J Conway, Alice Neal, Sophie Payne, Martin A Schwartz, Mukesh K Jain, Brian G Coon, Sarah De Val

Single-cell multiomic approaches define a gradual, spatially-regulated epigenetic and transcriptional transition from embryonic to adult neural stem cells
Beatrix S. Wang, Konstantina Karamboulas, Nareh Tahmasian, Daniel J. Dennis, David R. Kaplan, Freda D. Miller

Male fertility is independent of Enh13 control of Sox9 testicular expression
Maor Lubman, Meshi Ridnik, Isabelle Stévant, Yael Kimchi Djanshvili, Elisheva Abberbock, Shelly Ziv Lhermann, Nitzan Gonen

A dual-phase enhancer couples progenitor maintenance and pancreatic lineage stability
Marta Duque, João Amorim, Joana Teixeira, Beatriz Custódio, Mafalda Galhardo, Francisco Camões Magalhães, Joana Marques, Ana Paula Pêgo, José Bessa

Enhancer-mediated metabolic pre-patterning defines trabecular cardiomyocyte identity prior to morphogenesis
Costantino Parisi, Shikha Vashisht, Mohammad Salar Ghasemi Nasab, Kandhadayar Gopalan Srinivasan, Katarzyna Misztal, Marcin Zagorski, Cecilia Winata

ERH elicits cell lineage restriction in mammalian preimplantation development and differentiation from pluripotency via H3K9me3-heterochromatin
Andrew Katznelson, Blake Hernandez, Kylea Tapia, Holly Fahning, Adam Burton, Jingchao Zhang, Maria-Elena Torres-Padilla, Nicolas Plachta, Kenneth S. Zaret, Ryan L. McCarthy

A conserved C. elegans zinc finger-homeodomain protein, ZFH-2, continuously required for structural integrity and function of alimentary tract and gonad
Antoine Sussfeld, Berta Vidal, Surojit Sural, Daniel M. Merritt, G. Robert Aguilar, Yasmin Ramadan, Oliver Hobert

The Nkx2.3–Nr5a1 gene cascade plays a crucial role in spleen-specific vascular architecture and marginal zone formation
Kanako Miyabayashi, Koji Ono, Tetsuya Sato, Ayano Yahagi, Masanori Iseki, Katsuhiko Ishihara, Takami Mori, Miki Inoue, Ryuki Shimada, Kei-ichiro Ishiguro, Tomohiro Ishii, Jongsung Noh, Man Ho Choi, Takashi Baba, Yasuyuki Ohkawa, Emi Kiyokage, Kazunori Toida, Yuichi Shima

A Phospho-Switch for Cell Fate Control
Jin Ming, Xianzhuang Liu, Zexiao Jia, Wei Shi, Jiajun Li, Shikun Wang, Yulin Chen, Shixian Lin, Yu Liang, Peng Guo, Hanqing Zhao, Yuxiang Yao, Ruona Shi, Xiaofei Zhang, Yuanyue Shan, Yu Fu, Bo Wang, Chengchen Zhao, Duanqing Pei

Mitotic bookmarking by Prox1 preserves mammalian neuronal lineage identity memory via promoting timely H3K27me3 restoration
Chouin Wong, Jie Liu, Haoran Yang, Haotian Li, Xiaoqi Luo, Tingyi Li, Zili Chen, Jingyi Chu, Yuying Shen, Shuai Long, Yong Zhang, Yan Song

A Mediator-dependent hypertranscriptional program governs neural stem cell fate decisions in vivo
Tiago Baptista, Daniela Lopes, Ana Rita Rebelo, Catarina CF Homem

Single-Cell Atlas of Transcription and Chromatin States Reveals Regulatory Programs in the Human Brain
Yang Xie, Lei Chang, Guojie Zhong, Jonathan A. Rink, Tatiana Báez-Becerra, Ethan Armand, Wubin Ding, Kai Li, Eric Bonne, Audrey Lie, Hannah S Indralingam, Keyi Dong, Timothy Loe, Bohan Huang, Zhaoning Wang, Ariana S. Barcoma, Jackson K. Willier, Kyle W. Knutson, Jiayi Liu, Silvia Cho, Stella Cao, Kaitlyn G. Russo, Carissa K. Young, Jessica Arzavala, Yareli Sanchez, Aleksandra Bikkina, Natalie Schenker-Ahmed, Colin Kern, Zoey Zhao, Amit Klein, Jesus Flores, Chu-Yi Tai, Jacqueline Olness, Alexander Monell, Siavash Moghadami, Cesar Barragan, Chumo Chen, William Owens, Carolyn O’Connor, Michelle Liem, Mikayla V. Marrin, Cynthia Rose, Shane N. Alt, Nora Emerson, Julia Osteen, Jacinta Lucero, Daofeng Li, Rebecca D. Hodge, Ting Wang, C. Dirk Keene, Xiangming Xu, Quan Zhu, Joseph R. Ecker, M. Margarita Behrens, Bing Ren

DNA supercoiling links transcription and chromatin architecture during human stem cell differentiation
Consuelo Perez, Pierre Murat, Andrew Zeller, Kim C. Liu, Alastair Crisp, Julian E. Sale

Matched single-cell chromatin, transcriptome, and surface marker profiling captures in vivo epigenomic reprogramming during basal-to-luminal transition in the mammary gland
Anna Schwager, Eve Moutaux, Adeline Durand, Alexandra Van Keymeulen, Amélie Viaene, Mélanie Miranda, Louisa Hadj Abed, Simon Besson-Girard, Marion Lambault, Délia Dupré, Grégoire Jouault, Mélissa Saichi, Juliette Bertorello, Simon Dumas, Mathias Schwartz, Marthe Laisné, Justine Marsolier, Manuel Guthmann, Lorraine Bonneville, Urvashi Chitnavis, Déborah Bourc’his, Elisabetta Marangoni, Nicolas Servant, Cédric Blanpain, Leïla Perié, Céline Vallot

| Stem cells, regeneration & disease modelling

Neonatal diethylstilbestrol exposure disrupts uterine epithelial apical-basal polarity and partial EMT state
Rachel E. Bainbridge, Wendy N. Jefferson, Tianyuan Wang, Sara A. Grimm, Carmen J. Williams

PIK3CA-related overgrowth spectrum (PROS) zebrafish models reveal pan-lineage developmental dysregulation
Hannah Brunsdon, Nuoya Wang, Micha Sam Brickman Raredon, Ralitsa R Madsen, Robert K Semple, E Elizabeth Patton

Maternal-fetal immune conflict contributes to male-specific impairments in a mouse model of neurodevelopmental disorders
Irene Sanchez-Martin, Bharti Kukreja, Paige Henderson, Qianyu Lin, Daniel DiMartino, Valerie Bagan, Justin Park, Brian T. Kalish, Lucas Cheadle

PRDM16 is necessary for sensory neuronal development in the Trigeminal Ganglion
Fahmida Raha, Qiman Gao, Lomeli C. Shull, Kristin B. Artinger

Leucettinib-21 decreases dosage effects of DYRK1A in human trisomy 21 iPSC-derived neural cells
Nicole R. West, Mattias F. Lindberg, Julien Dairou, Shawn MacGregor, Sahith Puthireddy, Laurent Meijer, Anita Bhattacharyya

Mutant FGFR3 restricts bone yet expands cortex via ERK-mediated self-repression
Zhuangzhi Zhang, Zhejun Xu, Tongye Fu, Wenhui Zheng, Zizhuo Sha, Chuannan Yang, Feihong Yang, Jialin Li, Jing Ding, Zhengang Yang

Hypoxia differentially affects coronary vessel formation during heart development
Sophie Payne, Susann Bruche, Dorota Szumska, Alice Neal, Mark D Preston, Sarah De Val

Pancreatic Duct Cells as a Potential Source for Human Islet Neogenesis: Insights from Imaging Mass Cytometry
Rui Liang, Tengli Liu, Lanqiu Zhang, Wenmiao Ma, Huixia Ren, Shusen Wang

Rp-vasa: a bona fide Primordial Germ Cell marker that drives embryonic expression in the Chagas disease vector Rhodnius prolixus
G. Martins, M. Berni, T. Guedes-Silva, D. Bressan, J. Vieira, M. Cardoso, A. Pane, V. Gantz, E. Bier, H.M Araujo

Two axolotl-adapted cell-ablation platforms reveal macrophage-dependent processes essential for spinal-cord and skeletal regeneration
Gabriela Johnson, Andrew Hart, Markus Sujansky, Joel H. Graber, James W. Godwin

The regulatory landscape of optic fissure closure in the vertebrate eye
Brian Ho Ching Chan, Mariya Moosajee, Holly Hardy, James Prendergast, Joe Rainger

Evidence that injury can cause Drosophila gut differentiated, polyploid enterocytes to be recruited as stem cells via paligenosis
Dongkook Park, Robert M. Lawrence, Tyler Jackson, Hongjie Li, Jason C. Mills

The abnormal C-terminus in DVL1 impacts Robinow Syndrome phenotypes
Shruti S. Tophkhane, Gamze Akarsu, Sarah J. Gignac, Katherine Fu, Stephanie Xie, Esther M. Verheyen, Joy M. Richman

HSD17B7 is required for the function of sensory hair cells by regulating cholesterol synthesis
Yuqian Shen, Ziyang Wang, Xun Wang, Fuping Qian, Mingjun Zhong, Xin Wang, Jing Cheng, Dong Liu

Regeneration can take place across Drosophila compartments or segments with different Hox gene expression
Rafael Alejandro Juárez-Uribe, Paloma Martín, Laura Utiel, Blanca L. Arrabal, Marina Blanco, Roberto Yagüe-Serrano, Eduardo Cazalla, Ernesto Sánchez-Herrero

Intellectual disability risk gene RFX4 regulates cortical neurogenesis by restraining neuronal differentiation
Julianna J. Determan, Gareth Chapman, Sydney R. Crump, Faiza Batool, Sofia Malik, Taranjit S. Gujral, William Buchser, Caleb Valentine, Serena Elia, Monica Sentmanat, Xiaoxia Cui, Haley Jetter, Kristen L. Kroll

Brain morphological pattern is associated with the presence, severity, and transition of transdiagnostic psychiatric disorders in preadolescents
Nanyu Kuang, Christopher J Hammond, Betty Jo Salmeron, Xiang Xiao, Danni Wang, Laura Murray, Hong Gu, Tianye Zhai, Hui Zheng, Justine Hill, Maria Scavinicky, Hanbing Lu, Amy Janes, Thomas J Ross, Yihong Yang

4D Single-Cell Spatial Transcriptomics Reveals Dynamic Morphogenetic Gradients and Regenerative Domains in Planarians
Kai Han, Yue Chen, Yao Li, Lidong Guo, Yuxiaofei Wang, Xiawei Liu, Yaru Lin, Zhi Huang, Qun Liu, Wenjie Guo, Rui Zhang, Wandong Zhao, Langchao Liang, Xiaoyu Wei, Li Zhou, Xuebin Mao, Jiaqi Wang, Weijian Wu, Hongwei Pan, Tao Yang, He Zhang, Xiaoshan Su, Shanshan Liu, Wenwei Zhang, Longqi Liu, Søren Tvorup Christensen, Jifeng Fei, Xin Liu, Guangyi Fan, Hanbo Li, Ying Gu, Jian Wang, Huanming Yang, Gang Pei, Xun Xu, An Zeng, Mengyang Xu

Drosophila ryanodine receptor gene triggers functional and developmental muscle properties and could be used to assess the impact of human RYR1 mutations
Monika Zmojdzian, Teresa Jagla, Florian Cherik, Magda Dubinska-Magiera, Marta Migocka-Patrzalek, Malgorzata Daczewska, John Rendu, Krzysztof Jagla, Catherine Sarret

Asymmetric Histone Inheritance Regulates Olfactory Stem Cell Fates During Regeneration
Binbin Ma, Guanghui Yang, Jonathan Yao, Charles Wu, Jean Pinckney Vega, Gabriel Manske, Saher Sue Hammoud, Satrajit Sinha, Abhyudai Singh, Haiqing Zhao, Xin Chen

A knock-in Six2Cre line reveals transient interstitial potential in nephron progenitors
Azadeh Haghighitalab, Fariba Nosrati, Zeinab Dehghani-Ghobadi, Mohammed Sayed, Christopher Ahn, Yueh-Chiang Hu, Eunah Chung, Hee-Woong Lim, Joo-Seop Park

Lack of specificity of progenitor responses to injury in regeneration
Cecilia E. Pellegrini, Peter W. Reddien

Unique mineralization pattern revealed in TBCK syndrome mouse model
Kaitlin A. Katsura, Yuchen Jiang, Marius Didziokas, Nir Z. Badt, Sonia Dougherty, Kyle H. Vining, Elizabeth J. Bhoj

Pre-clinical models of idiopathic scoliosis implicate sex-specific roles for complement activity in modulating spinal curve severity
Vida Erfani, Brian Ciruna

Adipocyte-Derived Amino Acid Storage Proteins are Required for Germline Stem Cell Maintenance in Adult Drosophila Females
Anna B. Zike, Mekenzi O. Hazen, Madison G. Abel, Eleanor B. Goldstone, Robert C. Eisman, Lesley N. Weaver

Cell-autonomous Wnt activity promotes transient re-programming and cell cycle re-entry of coronary artery endothelial cells
Bhavnesh Bishnoi, Alfia Nirguni Saini, Vinay Rao, Omkar Golatkar, Ravindra Kailasrao Zirmire, Shruthi Viswanath, Perundurai Subramaniam Dhandapany, Soumyashree Das

Niche-dependent modular regulation of the stem cell transcriptome separates cell identity and potential
Amelie Raz, Hafidh Hassan, Yukiko Yamashita

TNAP and PHOSPHO1 function synergistically to afford critical control over the mineralisation of the postnatal murine skeleton
Lucie E Bourne, Aikta Sharma, Scott Dillon, Jacob Keen, Soher N Jayash, Natalie Crump, Lucinda AE Evans, Maya Karmali, Worachet Promruk, Claire E Clarkin, Sonoko Narisawa, Louise Stephen, Brian L Foster, José Luis Millán, Colin Farquharson, Katherine A Staines

Spinal cord regeneration deploys adult molecular programs that do not recapitulate embryonic development
Yuxiao Xu, Amulya Saini, Wenda Zhang, Lili Zhou, Mayssa H. Mokalled

Tissue composition shapes differential skeletal integration strategies during axolotl limb regeneration
Rita Aires, Sean D. Keeley, Kerstin Brandt, Mário Carreira, Doğa Berşan Güneş, Yagiz Savci, Ulrike Anne Friedrich, Andreas Dahl, Can Aztekin, Tatiana Sandoval-Guzmán

Endocardial TIE1 synergizes with TIE2 to regulate the atrial internal muscular network assembly
Kai Ding, Beibei Xu, Xinhao Yu, Xiwen Jia, Taotao Li, Xin Shen, Junda Li, Xudong Cao, Yahui Liu, Zhen Zhang, Yulong He

Hypoxia-activated scleraxis a mediates epicardial progenitor differentiation into a unique cardiac perivascular cell type
Björn Perder, Yu Xia, Jun Yao, Miaoyan Qiu, Alvin Gea Chen Yao, Muhammad Naeem, Paul Zumbo, Ignace Van der Wee, Avi Yakubov, Kazu Kikuchi, Doron Betel, Todd Evans, Michael R. Harrison, Jingli Cao

| Plant development

A Functional Basis for the Developmental Sequence of the Macrostructure of the Venus Flower Basket (Euplectella aspergillum)
Y. Mistry, S. Morankar, D. Kingsbury, N. Chawla, C. A. Penick, D. Bhate

Naturally occurring variation in gene-associated transposable elements impacts gene expression and phenotypic diversity in woodland strawberry
Santiago Priego-Cubero, Rocio Tolley, Julia Llinares-Gómez, Camila Zlauvinen, Tuomas Toivainen, Timo Hytönen, Carmen Martín-Pizarro, Ileana Tossolini, Pablo A. Manavella

Toxic metals increase root hair density by reducing epidermal cell length
Julia Zheku, Thea Do, M. Arif Ashraf

Extracellular calcium modulates pollen tube growth and guidance in Arabidopsis thaliana
Kumi Matsuura-Tokita, Yoko Mizuta, Daisuke Kurihara, Tetsuya Higashiyama

WUSCHEL modulates jasmonate signaling to control the balance between growth and defense in the shoot apical meristem
Pengfei Fan, Panagiotis Boumpas, Christian Wenzl, Yanfei Ma, Gernot Poschet, Jiao Zhao, Thomas Greb, Jan U. Lohmann

Mechanical coordination of counter-gradient growth maintains organ curvature in the apical hook
Sara Raggi, Hemamshu Ratnakaram, Adrien Heymans, Loitongbam Lorinda Devi, Özer Erguvan, Siamsa M. Doyle, François Jobert, Asal Atakhani, Sijia Liu, Manuel Petit, Jürgen Kleine-Vehn, Krzysztof Wabnik, Stéphane Verger, Stéphanie Robert

A visualization framework for cell division activity and orientation in pre-anthesis ovaries of Prunus species
Ayame Shimbo, Soichiro Nishiyama, Tatsuya Katsuno, Akane Kusumi, Hisayo Yamane, Masahiro M. Kanaoka, Ryutaro Tao

Transformers Outperform ConvNets for Root Segmentation: A Systematic Comparison Across Nine Datasets
Abraham George Smith, Sotiris Lamprinidis, Anand Seethepalli, Larry M. York, Eusun Han, Patrick Möhl, Kyriaki Boulata, Kristian Thorup-Kristensen, Jens Petersen

Evolution of moss leaf-like organs through variations in deeply conserved developmental principles
Wenye Lin, Loann Collet, Laure Mancini, Mandar Deshpande, Brendan Lane, Benjamin P. Lapointe, Agnieszka Bagniewska-Zadworna, Anne-Lise Routier-Kierzkowska, Richard S. Smith, Yoan Coudert, Daniel Kierzkowski

Ca²⁺ oscillations promote microtubule-band turnover and support tip growth in Arabidopsis zygotes
Hikari Matsumoto, Zichen Kang, Tomonobu Nonoyama, Yusuke Kimata, Satoru Tsugawa, Minako Ueda

Effects of lovastatin on auxin transport and root development in Arabidopsis thaliana
Veronica Giourieva, Christos Tersenidis, Alkiviadis Athanasiadis, Stylianos Poulios, Anna Kouskouveli, Konstantinos Vlachonasios, Emmanuel Panteris, George Komis

| Eco-evo-devo

Ancient origin of the dorso-ventral patterning system of vertebrate paired fins
Rebecca E. Dale, Silke Berger, Sara Alaei, Adele Barugahare, Marcus C. Davis, Laura Perlaza-Jimenez, Frank J. Tulenko, Peter D. Currie

Embryonic and larval development of the Pacific saury Cololabis saira: Distinctive characteristics of a rapidly growing beloniform fish
Rie Kusakabe, Shinya Yamauchi, Shigehiro Kuraku

Age- and Light-Dependent Changes in the Zebrafish Olfactory Epithelium
George B. Chapman, Rania Abutarboush, Victoria Connaughton

Thrifty phenotypes in ants: Extending a human developmental hypothesis to a superorganism
Érik Plante, Ehab Abouheif, Jean-Philippe Lessard

Thermal Plasticity of Stage-specific Development Time and Adult Body Size under Temperature Shifts: A Case Study Using Drosophila melanogaster
Aradhya Chattopadhyay, Rishav Roy, Payel Biswas, Shampa M. Ghosh

The dynamic evolution of panarthropod germ cell specification mechanisms
Jonchee A. Kao, Emily L. Rivard, Rishabh R. Kapoor, Cassandra G. Extavour

Annelid eye evolution revealed by developmental, ultrastructural, and connectome analyses of cerebral eyes in Malacoceros fuliginosus
Suman Kumar, Anna Seybold, Oleg Tolstenkov, Sharat Chandra Tumu, Harald Hausen

Tracking morphological development in stony corals
Garrett J. Fundakowski, Viviana Brambilla, Kyle J. A. Zawada, Cher F Y Chow, Emily Croasdale, Amelia J. F. Errington, Luisa Fontoura, Wilhelm J Marais, Rachael M. Woods, Pim Edelaar, Kevin Lala, Joshua S. Madin, Maria Dornelas

Cell Biology

Whole-Cell Proteomics Identifies Novel Regulators of Ciliogenesis Beyond the Axoneme
Xiaolu Xu, Yanbao Yu, Tony Zheng, Fiona Clark, Jean Ross, Neha Sindhu, Andre L P Tavares, John B Wallingford, Shuo Wei, Jian Sun

Developmentally programmed nuclear pore complex replacement enables oocyte specification
Shruti Venkat, Tram Nguyen, Cecilia Blangini, Michelle Pollak, Karen Schindler, Maya Capelson, Prashanth Rangan

PKN is a sex- and species-specific fertilization factor in brown algae
Masakazu Hoshino, Meri Nehlsen, Rita A. Batista, Morgane Raphalen, Toshiyuki Wakimoto, Shinya Uwai, Kazuhiro Kogame, Vikram Alva, Susana Coelho

HDAC1/2-mediated repression of Wnt receptor expression orients asymmetric division polarity in C. elegans
Mar Ferrando-Marco, Beatriz Garcia del Valle, Mark Hintze, Lucy Narunsky, Shuxiao Lin, Junyue Huang, Shannon Edwards, Michalis Barkoulas

Forward Programming Identifies Inducers of Blood-Brain Barrier Properties in Human Pluripotent Stem Cell-Derived Endothelial Cells
Soniya Tamhankar, Yunfeng Ding, Fatemeh Yaghoobi Hashjin, Sarah M. Boutom, Richard Daneman, Sean P. Palecek, Eric V. Shusta

Restoration of Spermatogenesis is Dependent on Activation of a SPRY4-ERK Checkpoint Following Germline Stem Cell Damage
Ying Liu, Tansol Choi, Brad Pearson, Ryan Nachman, Whitney Woo, Na Xu, Ryan Schreiner, Romulo Hurtado, Marco Seandel, Shahin Rafii, Todd Evans

Microtubules sustain the fidelity of cellularization in a coenocytic relative of animals
Margarida Araújo, Marine Olivetta, Paolo Ronchi, Viola Oorschot, Arif Khan, Christian Tischer, Hiral Shah, Gautam Dey, Omaya Dudin

Mechanosensing and IL-13 Signaling Synergistically Modulate Intestinal Stem Cell Differentiation via STAT6 and YAP
Sarbari Saha, Thao Nguyen, Cornelis Mense, Marie Touzet-Robin, Karen Kresbach, Stephan A. Eisler, Ulrich S. Schwarz, Andrew G. Clark

Altered stem cell properties of human hematopoietic stem and progenitor cells based on bone region location
Christopher J. Wells, Christine Hall, Samantha M. Holmes, Isabelle J. Grenier-Pleau, John F. Rudan, Steve Mann, Sheela A. Abraham

Modelling

Segmented wavetrains and sites of reversal in the mouse seminiferous tubules
Kei Sugihara, Ayuki Sekisaka, Toshiyuki Ogawa, Takashi Miura

Early development of male germ cell clones shapes their reproductive success
Tatsuro Ikeda, Maurice Langhinrichs, Tamar Nizharadze, Chieko Koike, Yuzuru Kato, Katsushi Yamaguchi, Shuji Shigenobu, Kana Yoshido, Shinnosuke Suzuki, Toshinori Nakagawa, Ayumi Maruyama, Seiya Mizuno, Satoru Takahashi, Nils B. Becker, Hans-Reimer Rodewald, Thomas Höfer, Shosei Yoshida

A mathematical synthesis of genetics, development, and evolution
Mauricio González-Forero

Tools & Resources

Cellular diversity of the developing chick trigeminal ganglion at single-cell resolution
Arvind Arul Nambi Rajan, Erica J. Hutchins

A quantitative in vivo CRISPR-imaging platform identifies regulators of hyperplastic and hypertrophic adipose morphology in zebrafish
Rebecca Wafer, Panna Tandon, James E. N. Minchin

A robust human airway organoid platform enables scalable expansion and trajectory mapping of pulmonary neuroendocrine cells
Noah Candeli, Lisanne den Hartigh, Nicholas Hou, Andrés Marco, José Antonio Sánchez-Villicaña, Andrea García-González, Shashank Gandhi, Francesca Sgualdino, Alyssa J. Miller, Jason Spence, Susana Chuva de Sousa Lopes, José L. McFaline-Figueroa, Hans Clevers, Talya L. Dayton

Nuclear Histone 3 Post-Translational Modification Profiling in Whole Cells using Spectral Flow Cytometry
Carly S. Golden, Saylor Williams, Sandeep Sreerama, Sophia Blankevoort, H. Joseph Yost, Martin Tristani-Firouzi, Anna Belkina, Maria A. Serrano

Epitope-based labeling for improved live-imaging of endogenous proteins in C. elegans
Elise van der Salm, Mette H. Schroeder, Loes B. Steller, Stephanie I. Miller, Amelie Scheper, Gwen Nowee, Erik. E. Griffin, Suzan Ruijtenberg

A facile method for fluorescent visualization of newly synthesized fibrous collagen by capturing allysine aldehyde groups as cross-link precursors
Junpei Kuroda, Kazunori K. Fujii, Sugiko Futaki, Azumi Hirata, Yuki Taga, Takaki Koide

Electrical stimulation combined with p27Kip1 inactivation drives proliferative neurogenic reprogramming of Mueller glia in the adult mouse retina
Megan L. Stone, Joel Jovanovic, Edward M. Levine

Arrayed single-gene perturbations identify drivers of human anterior neural tube closure
Roya E. Huang, Giridhar M. Anand, Heitor C. Megale, Jason Chen, Chudi Abraham-Igwe, Sharad Ramanathan

Conserved cellular architecture and developmental mechanisms of the zebrafish meninges
Ashley L. Arancio, Kathryn Wilhem, Hung-Jhen Chen, Brandon M. Hernandez, Percy J. Raggi, D’Juan T. Farmer

A Single-Cell Temporal Atlas of Mouse Nasal Embryonic Development
Huan Chen, Yingxiu Chen, Mengjie Pan, Ziyu Feng, Baomei Cai, Yiyi Cheng, Sihao Chen, Jiehong Deng, Xia Yao, Chunhua Zhou, Yunjing Du, Wei He, Ruifang Zhang, Yudong Fu, Shujuan Liu, Lihui Lin, Shengyong Yu, Yuehong Yan, Duanqing Pei, Dajiang Qin, Jiekai Chen, Shangtao Cao

Cell Type Architecture and Positional Gene Gradients in an Adult Animal at Subcellular Resolution
Maoqin Sun, Yuxiaofei Wang, Kai Han, Lidong Guo, Yue Chen, Yao Li, Yaru Lin, Xiawei Liu, Zhi Huang, Qun Liu, Wenjie Guo, Rui Zhang, Wandong Zhao, Langchao Liang, Xiaoyu Wei, Li Zhou, Xuebin Mao, Jiaqi Wang, Weijian Wu, Hongwei Pan, Tao Yang, He Zhang, Xiaoshan Su, Shanshan Liu, Wenwei Zhang, Longqi Liu, Søren Tvorup Christensen, Jifeng Fei, Xin Liu, Ying Gu, Jian Wang, Huanming Yang, Gang Pei, Guangyi Fan, Xun Xu, Hanbo Li, Mengyang Xu, An Zeng

Husbandry and Maintenance of Carausius morosus Laboratory Populations
Macy Ingersoll, Petra Kovacikova, Yousuf Hashmi, Cassandra G. Extavour

A molecular and spatial resource defining tubulin isotype organization during corneal development
R Ramarapu, WR Stoehr, M Miesen, S. Border, SM Thomasy, CD Rogers

Efficient derivation and transcriptional characterization of mouse extra-embryonic endoderm stem cell lines generated by somatic cell nuclear transfer
Shuaipeng Li, Shu Wei, Guomeng Li, Mei Hu, Jiangwei Lin, Wandong Bao

Integrative Inference of Spatially Resolved Cell Lineage Trees using LineageMap
Xinhai Pan, Yiru Chen, Xiuwei Zhang

Observing concurrent subcellular dynamics in large living tissues
Charles S Wright, Sanjeev Uthishtran, Laura Z Kreplin, Hetvi R Gandhi, Abhishek Patil, Harrison M York, Samyukta Sita, Samuel A Manning, Elliot Brooks, Guizhi Sun, In-won Lee, Wing Hei Chan, Sara Hlavca, Samuel Crossman, Helen E Abud, Jan Kaslin, Avnika A Ruparelia, Peter D Currie, Kieran F Harvey, Jose M Polo, John Carroll, Senthil Arumugam

High resolution spatial transcriptomic and proteomic profiling of early primate gastrulation in utero
Nikola Sekulovski, Maliha Kabir, Anusha Rengarajan, Amber E. Carleton, Jenna K. Schmidt, Chien-Wei Lin, Kenichiro Taniguchi

Single-cell-scale spatial transcriptome reveals early regional priming of the developing mouse ovary
Anthony S. Martinez, Tyler J. Gibson, Courtney Diamond, Jennifer Jaime, Jennifer McKey

Efficient multi-lineage cardiovascular differentiation of human pluripotent stem cells in animal serum-free conditions
Nguyen T N Vo, Kelvin Chung, Aishah Nasir, Davor Pavlovic, Chris Denning

Adapting the OpenFlexure Microscope for Affordable Live-Cell Imaging
Jodie R Malcolm, Olympia Physouni, Stuart Lacy, Mark Bentley, Stephen P Howarth, Sandy MacDonald, Alastair P Droop, Benedict Powell, Laura Wiggins, William J Brackenbury, Peter J O’Toole

Direct, high-throughput linking of single-cell imaging and gene expression
Catherine K Xu, Georg Meisl, Nikita Moshkov, Niklas A Schmacke, Karolis Goda, Alexey Shkarin, Maximilian F Schlögel, Tuomas PJ Knowles, Fabian J Theis, Linas Mazutis, Jochen Guck

A live-imaging system for Arabidopsis leaf primordia at early stages
Yujie Zhao, Hokuto Nakayama, Satohiro Okuda, Tetsuya Higashiyama, Hirokazu Tsukaya

NucVerse3D: Generalizable 3D nuclear instance segmentation across heterogeneous microscopy modalities
Jorge Vergara, Cristian Perez-Gallardo, Ricardo Velasco, Dilan Martinez, Diego Badilla, Esteban G. Contreras, Pamela Guevara, Fabián Segovia-Miranda, Hernán Morales-Navarrete

Super-resolution single-cell spatial atlas of plant de novo regeneration
Xiehai Song, Shaoman Zhang, Zhiliang Yue, Yongqi Liu, Shanshan Chen, Yani Niu, Yan Shi, Hengjia Yang, Li Xu, Naixu Liu, Yuanyuan Miao, Man Lv, Jinshan Li, Tong Wang, Meizhi Xu, Binmei Sun, Chuan Qiu, Ruirui Xu, Jizong Wang, Huawei Zhang, Shuguo Hou, Gang Li, Haodong Chen, Xing Wang Deng, Bosheng Li

Long-term ex ovo culture of Caenorhabditis elegans embryos
Clover Ann Stubbert, Cherry Soe, Pavak Kirit Shah

A Data-Driven Image Extraction and Analysis Pipeline for Plant Phenotyping in Controlled Environments
Fahimeh Orvati Nia, Joshua Peeples, Seth C. Murray, Andrew McFarland, Troy Vann, Shima Salehi, Robert Hardin, David D. Baltensperger, Amir Ibrahim, J. Alex Thomasson, Henry Fadamiro, Nithya K Subramanian, Nazar Oladepo, Uday Vysyaraju

Research practice & education

Set-up, validation, evaluation, and cost-benefit analysis of an AI-assisted assessment of responsible research practices in a sample of life science publications
Silke Kniffert, Ben Katthöfer, Robert Emprechtinger, Pasquale Pellegrini, Eva Maria Funk, Ishminder Singh Dhamrait, Yalei Zang, Ailyn Bornmüller, Ulf Toelch

Science should be machine-readable
A. Sina Booeshaghi, Laura Luebbert, Lior Pachter

bioRxiv: the preprint server for biology
Richard Sever, Samantha Hindle, Ted Roeder, Sol Fereres, Olaya Fernández Gayol, Sanchari Ghosh, Martina Proietti Onori, Emma Croushore, Kevin-John Black, Linda Sussman, Janet Argentine, Wayne Manos, Marisol Muñoz, Josh Sinanan, Tracy K. Teal, John R. Inglis

Benefits and Challenges of Integrating a Generative AI Assisted Reading Guide in an Undergraduate Journal Club Assignment
Ashley Ringer McDonald, Anne V. Vázquez

The post February in preprints appeared first on the Node.

Greetings to the Node community, 

My name is Andrea Murillo, and I am delighted to share that I am the new Community Manager for the Node. I started my research journey as a physiologist and later found my way into endocrinology during my PhD, where I worked with my favourite worm and developmental model species, Capitella teleta. Throughout my PhD, I investigated components of the estrogen signalling pathway across life stages of C. teleta. That is how the wonder of developmental biology first wormed its way into my heart.  

After finishing my PhD, I started working for The Company of Biologists as the Science Communications Officer. In that role, my passion for science communication grew, and my appreciation for biology deepened as I wrote about the Company’s fantastic journal content across many fields. But it was the science and the community surrounding the Node and Development that truly hooked me (I will stop with the worm puns now!). 

As Community Manager, I am excited to build on the great work done by Eva, Cat, Aidan, Helen and Joyce and to continue some of our users’ favourite series and features. In my previous role, I worked closely with both my predecessor, Joyce, and the two Community Managers from our sister sites: Reinier from preLights and Helen from FocalPlane. I am thrilled to be a part of the team as the Node Community Manager, a transition which they have generously supported. I’m planning to bring some of my own fresh ideas to the Node, and I hope you will like them.  

My first chance to meet some of you in person will be at the British Society for Developmental Biology 2026 Spring Meeting next week. If you are attending, please stop by and say hello. I am really excited to learn about your research and, most of all, to meet the people who make great developmental biology and stem cell research happen.  

Wormest regards, 

Andrea 

The post Hi from the new Community Manager appeared first on the Node.

Preprints have become an indispensable part of our research ecosystem. Over the last 10 years, the biological community has witnessed an exponential growth in both submissions and readership of preprints. Arguably, the main drivers behind this growth are the ability of preprints to speed up the dissemination of research and broaden access to results long before formal publication.

Development and our not-for-profit publisher, The Company of Biologists, have a long history of actively supporting preprints (Prosée and Brown, 2025). In 2018, the Company launched preLights, a community-run platform that highlights noteworthy preprints across the biological sciences. Over time, preLights has evolved to provide support and training for early-career researchers to develop their writing skills for summarising and critiquing new work. In addition, Development’s own community site, the Node, posts monthly preprint lists from developmental biology and related fields. These lists are among the most-read posts on the Node, demonstrating the value of preprints within our community. Over time, preLights, the Node and Development have started working together more closely to highlight noteworthy preprints; a recent collaboration between preLights and the Node saw the introduction of curated preprint highlights in the form of ‘preLighters’ choice’ posts and a selection of preLights posts from the stem cell and developmental biology community feature in the journal as quarterly ‘Preprint Highlights’.

As part of this preprint ecosystem, Development launched its ‘In preprints’ series in early 2022 to bring curated, contextualised coverage of preprinted findings directly to our readership (Briscoe and Grewal, 2022). These articles are intended to complement other initiatives, such as preLights, in guiding readers to the preprints that matter the most in the field. We know that Development’s ‘In preprints’ articles receive, on average, over a thousand views within the first 12 months of publication and continue to be read in the years that follow. Development has now published around 60 ‘In preprints’ articles on topics ranging from single-cell lineage tracing techniques (Rodriguez-Fraticelli and Morris, 2022) to human stem cell-based embryo models (Moris and Sturmey, 2023) and Polycomb complexes (Iwasaki et al., 2023) to leaf-shape transitions (Byrne, 2024). You can browse all the ‘In preprints’ articles published to date in our dedicated subject collection.

Preprints featured in these articles have mainly been selected by Development’s in-house Reviews Editors. We are now expanding this initiative by appointing a small group of Preprint Editors – active researchers with their finger on the pulse of preprint literature – to commission and write ‘In preprints’ articles. This is an exciting opportunity to co-curate the preprint literature directly with members of the Development community, bringing specialist expertise and diverse perspectives to bear on an ever-growing body of work. We intend to appoint Preprint Editors who represent specialist topics within the broader scope of Development, and we hope that, as expert academics, they will be able to identify and highlight the best preprints from their fields as part of their routine exploration of the research literature.

We are currently accepting applications for Preprint Editors, and our call will close on Monday 30 March 2026. Applicants with at least 3 years of postdoctoral experience or principal investigators from any career stage are welcome to apply. In exchange for their contributions to the project, Preprint Editors will receive formal recognition and financial remuneration, including the option to receive support to attend relevant conferences. To find out more about this initiative, please visit our webpage: https://journals.biologists.com/dev/pages/preprint-editors.

We look forward to working with our first Preprint Editors following their appointment later this year to further strengthen our coverage of important preprinted work. We also anticipate that this programme will continue to evolve in response to community needs. This next step marks our commitment to supporting the reach of preprinted work and bringing curated, quality research to the attention of our community.

The post Preprint Editors – Development’s next step into the preprint landscape appeared first on the Node.

Biology Open is proud to partner with the European Zebrafish Society (EZS) to offer travel grants for the 13th European Zebrafish Meeting (EZM2026) to be held 7-11 July 2026 in Vienna, Austria. This travel grant is designed to provide financial support for early-career researchers based in the Global South.

The aim is to strengthen links for future collaboration and enhance the researcher’s career opportunities, which is in line with The Company of Biologists’ core value of supporting biologists.

More information and an application form can be found on the EZS website.

Deadline to apply: 15 March 2026

The post BiO Global South Travel Grants for EZM2026 appeared first on the Node.

The preprints this month are hosted on bioRxiv – use these links below to get to the section you want:

Developmental biology

• Patterning & signalling
• Morphogenesis & mechanics
• Genes & genomes
• Stem cells, regeneration & disease modelling
• Plant development
• Environment, evolution and development

Cell Biology

Modelling

Tools & Resources

Research practice and education

Developmental biology

| Patterning & signalling

Notch signaling in the embryonic ectoderm promotes periderm cell fate and represses mineralization of vibrissa hair follicles
Dianzheng Zhao, Yunus Ozekin, Erin Binne, Irene Choi, Aftab Taiyab, Trevor Williams, Hong Li

A Genetic Mechanism Linking Hippo Signaling to Dorsoventral Patterning for Control of Head and Eye Development
Basavanahalli Nunjundaiah Rohith, Neha Gogia, Arushi Rai, Amit Singh, Madhuri Kango-Singh

An APP-centered molecular gateway integrates innate immunity and retinoic acid signaling to drive irreversible metamorphic commitment
Ryohei Furukawa, Mizuki Taguchi, Narufumi Kameya, Keisuke Tanaka, Haruka Sato, Takehiko Itoh, Yuh Shiwa

MEIS1 is Required for Establishing Bergmann Glia–Specific Properties in the Developing Cerebellum
Kentaro Ichijo, Toma Adachi, Tomoo Owa, Minami Mizuno, Kyoka Suyama, Kaiyuan Ji, Koichi Hashizume, Ikuko Hasegawa, Eriko Isogai, Masaki Sone, Yukiko U. Inoue, Ryo Goitsuka, Takuro Nakamura, Takayoshi Inoue, Satoshi Miyashita, Kenji Kondo, Tatsuya Yamasoba, Mikio Hoshino

Cell position is more important than cell shape or age for the acquisition of cell identity in the brown alga Ectocarpus
Denis Saint-Marcoux, Bernard Billoud, Sabine Chenivesse, Carole Duchêne, Aude Le Bail, Jane A. Langdale, Bénédicte Charrier

The Joubert gene TMEM67 is required for the correct establishment of spinal dorsal identities in human organoids
Wiegering, K. Bools, I. Anselme, L. Metayer-Derout, O. Mercey, E. Balissat, Y. Bijek, M. Catala, S. Schneider-Maunoury, A. Stedman

Single-Cell Profiling of the Developing Organ of Corti Identifies Etv4/5/1 as Key Regulators of Pillar Cell Identity
Susumu Sakamoto, Matthew W. Kelley

Coordinated inhibition of SOX9 and cell cycle progression by microRNA-200 restricts sebaceous gland fate specification
Arpan Das, Yuheng C Fu, Haimin Li, Megan A. Wong, Annalina Che, Anumeha Singh, Jimin Han, Glen Bjerke, Dongmei Wang, Rui Yi

Smoothened turnover regulated by Hedgehog signaling in Drosophila
Ryo Hatori, Wanpeng Wang, Thomas B. Kornberg

Lipid-mediated reinforcement of FGF/MAPK signaling enables robust otic placode specification
Stephanie R. Peralta, Natalia Maiorana, Michael L. Piacentino

Evidence of autonomous neural specification for both brain and ventral nerve cord tissue in Annelida
Nicole B. Webster, Johnny A. Davila-Sandoval, Allan M. Carillo-Baltodano, Skyler Duda, B. Duygu Özpolat, Néva P. Meyer

| Morphogenesis & mechanics

Force-dependent stabilization of apical actomyosin by Lmo7 during vertebrate neurulation
Miho Matsuda, Sergei Y. Sokol

Depletion of S100A4+ stromal cells results in abnormal nipple development and nursing failure
Denisa Jaros Belisova, Ema Grofova, Viacheslav Zemlianski, Zuzana Sumbalova Koledova

A Cell Size-Dependent Competition Between Geometry and Polarity Governs Nuclear and Spindle positioning in Early Embryos
Aude Nommick, Macy Baboch, Celia Municio-Diaz, Jeremy Sallé, Remi Le Borgne, Nicolas Minc

Extrinsic MMPs drive epithelial shape change via basal ECM disassembly in the Drosophila wing disc
Chigusa Hinata, Hirotatsu Nakagawa, Shigeaki Nonaka, Katsuya Nozaki, Yoshikatsu Sato, Shizue Ohsawa

A collagen orientation switch reshapes fin architecture
Rintaro Tanimoto, Kazuhide Miyamoto, Koji Tamura, Shigeru Kondo, Junpei Kuroda

Recognizing dUTPase as a mitotic factor essential for early embryonic development
Nikolett Nagy, Otília Tóth, Eszter Oláh, László Henn, Gergely Attila Rácz, Edit Szabó, György Várady, Fanni Beatrix Vigh, Zita Réka Golács, Martin Urbán, Tímea Pintér, Orsolya Ivett Hoffmann, László Hiripi, Hilde Loge Nilsen, Angéla Békési, Miklós Erdélyi, Elen Gócza, Gergely Róna, Judit Tóth, Beáta G. Vértessy

Planar polarization of endogenous ADIP during Xenopus neurulation
Satheeja Santhi Velayudhan, Keiji Itoh, Chih-Wen Chu, Dominique Alfandari, Sergei Y. Sokol

RNA Polymerase III subunit Polr3a is required for craniofacial cartilage and bone development
Bailey T. Lubash, Roxana Gutierrez, Kade Fink, Colette A. Hopkins, Jessica C. Nelson, Kristin E.N. Watt

Cell-cycle inhibition preserves robust development but rebalances lineages in mouse gastruloids
Maxine Leonardi, Yves Paychère, Felix Naef

Distinct roles of the Lyve1 lineage in heart development
Konstantinos Klaourakis, Karolina Zvonickova, Jacinta Kalisch-Smith, Nicola Smart, Duncan Sparrow, David G. Jackson, Paul R. Riley, Joaquim M. Vieira

Spatial Variation in Cortex Glia Cell Cycle Supports Central Nervous System Organization in Drosophila
Vaishali Yadav, Syona Tiwari, Meenal Meshram, Ramkrishna Mishra, Rakesh Pandey, Richa Arya

| Genes & genomes

IAP retrotransposons contribute to the transcriptional diversity of the murine placenta
Samuele M. Amante, Maria L. Vignola, Cyril Pulver, Tessa M. Bertozzi, Anne C. Ferguson-Smith, Marika Charalambous, Miguel R. Branco

MYRF drives heterochronic miRNAs and LIN-42, and amplifies oscillatory programs for stage transitions
Zhao Wang, Shiqian Shen, Xiaoting Feng, Di Chen, Qian Bian, Yingchuan B. Qi

Yin Yang 1-Dependent PcG Function is Essential for TET2 Expression and Early T cell Development
Yinghua Wang, Sahitya Saka, Xuan Pan

A role for HDAC3 in regulating histone lactylation and maintaining oocyte chromatin architecture and fertility
Inês Simões-Gomes, António Jacinto, Ana Pimenta-Marques

The histone code of love: epigenetics of maturation of gonads in the human blood fluke Schistosoma mansoni
Christoph Grunau, Zhigang Lu, Avril Coghlan, Max Moescheid, Thomas Quack, Cristian Chaparro, Eerik Aunin, Jean-Francois Allienne, Adam Reid, Nancy Holroyd, Matt Berriman, Gilda Padalino, Karl F. Hoffmann, Christoph G. Grevelding, Ronaldo de Carvalho Augusto

Histone H3K9 Methyltransferases Regulate Cortical Growth by Coordinating Heterochromatin Formation and Neural Progenitor Dynamics
Sophie Warren, Chris Hemmerich, Ram Podicheti, José-Manuel Baizabal

A developmental timer coordinates organism-wide microRNA transcription
Peipei Wu, Jing Wang, Brett Pryor, Isabella Valentino, David F. Ritter, Kaiser Loel, Justin Kinney, Sevinc Ercan, Leemor Joshua-Tor, Christopher M. Hammell

Identification of germline chromatin modifying factors that influence zygotic transcription activation in C. elegans
Mariateresa Mazzetto, Paige Adekplor, Valerie Reinke

The SynMuvA lin-15A licenses natural transdifferentiation by antagonizing identity safeguarding mechanisms
Sarah Becker, Marie-Charlotte Morin, Julien Lambert, Shashi Kumar Suman, Francesco Carelli, Alex Appert, Stéphane Roth, Sarah Hoff-Yoessle, Jessica Medina-Sanchez, Manuela Portoso, Stéphanie Le Gras, Julie Ahringer, Sophie Jarriault

Decoding the cell intrinsic and extrinsic roles of PRC2 in early embryogenesis
Chengjie Zhou, Meng Wang, Zhiyuan Chen, Yi Zhang

Endogenous retroviral elements LTR8B and MER65 regulate the PSG9 locus that promotes trophoblast syncytialization Insights into placental evolution and pre-eclampsia pathology
Manvendra Singh, Yuliang Qu, Amit Pande, Julianna Zadora, Florian Herse, Martin Gauster, Xuhui Kong, Rongyan Zheng, Rabia Anwar, Katarina Stevanovic, Ralf Dechend, Marie Cohen, Attila Molvarec, Jichang Wang, Miriam K. Konkel, Bin Zhang, Cedric Feschotte, Gabriela Dveksler, Sandra M. Blois, Laurence D. Hurst, Zsuzsanna Izsvák

Single-cell spatially resolved transcriptomic characterization of the developing mouse cochlea
Philippe Jean, Sabrina Mechaussier, Amrit Singh-Estivalet, Céline Trébeau, Aurore Gaudin, Laura Barrio Cano, Andrea Lelli, Fabienne Wong Jun Tai, Sébastien Megharba, Sandrine Schmutz, Sarra Loulizi, Sophie Novault, David Hardy, Carolina Moraes-Cabe, Milena Hasan, Christine Petit, Raphael Etournay, Nicolas Michalski

A conserved C. elegans zinc finger-homeodomain protein, ZFH-2, continuously required for structural integrity and function of alimentary tract and gonad
Antoine Sussfeld, Berta Vidal, Surojit Sural, Daniel M. Merritt, G. Robert Aguilar, Yasmin Ramadan, Oliver Hobert

let-7 miRNA and lin-46 mRNA are the two essential targets of the LIN28 RNA-binding protein in developmental timing
Jana Brunner, Anca Neagu, Dimos Gaidatzis, Lucas J. Morales Moya, Helge Großhans

Conserved Roles of Sp1 in Zebrafish Development and Early Organogenesis
Ankita Sharma, Sudiksha Mishra, Greg Jude Dsilva, Saurabh J Pradhan, Pavan Dev Govardhan, Sanjeev Galande

An Asynchronous Production Line of Meiotic Prophase I in the Mouse Fetal Ovary
Chang Liu, Ziyi Jin, Gan Liu, Guofeng Feng, Jie Li, Yiwei Wu, Hao Jia, Lin Liu

Aging, dauer, and stature phenotypes are conferred by structure-directed missense mutations in the endogenous AGE-1/phosphatidylinositol 3-kinase catalytic subunit
You Wu, Tam Duong, Neal R. Rasmussen, Kent L. Rossman, David J. Reiner

Mapping of CELF1-RNA interactions reveals post-transcriptional control of lens development
Justine Viet, Matthieu Duot, Agnès Méreau, Yann Audic, Iwan Jan, David Reboutier, Catherine Le Goff-Gaillard, Sarah Y Coomson, Salil A Lachke, Carole Gautier-Courteille, Luc Paillard

microRNAs affecting development of body pigmentation in adult Drosophila melanogaster
Abigail M. Lamb, Jennifer A. Kennell, Eden W. McQueen, Evan J. Waldron, Patricia J. Wittkopp

Identification of miR-187 as a modulator of early oogenesis and female fecundity in medaka
Marlène Davilma, Stéphanie Gay, Manon Thomas, Sully Mak, Fabrice Mahé, Laurence Dubreil, Jérôme Montfort, Aurélien Brionne, Julien Bobe, Violette Thermes

Parthenogenote-Derived Brain Unveils the Critical Role of Paternal Genome in Neural Development
Marina Takechi, Yezhang Zhu, Zezhen Lu, Ying Zeng, Ken-Ichi Mizutani, Toru Nakano, Li Shen, Shinpei Yamaguchi

A panoramic view of the expression and function of the Doublesex/DMRT gene family in C. elegans
Chen Wang, Yehuda Salzberg, Meital Oren-Suissa, Oliver Hobert

Lamin A/C directs nucleosome-scale chromatin remodeling to define early lineage segregation in mammals
Alice Sherrard, Liangwen Zhong, Caroline Hoppe, Srikar Krishna, Scott Youlten, Curtis W. Boswell, Stephen Cross, Fiona E. Sievers, Goli Ardestani, Denny Sakkas, Liyun Miao, Zachary D. Smith, Berna Sozen, Antonio J. Giraldez

| Stem cells, regeneration & disease modelling

Prenatal corticosteroid exposure disrupts vascular-immune interactions and impairs steroidogenesis in the fetal testis
Satoko Matsuyama, Lauren Hudepohl, Kazuhiro Matsuyama, Shu-Yun Li, Meghana Ginugu, Xiaowei Gu, Matthew J. Kofron, Vikram Ravindra, Tetsuo Shoda, Tony DeFalco

Hyaluronan underlies the emergence of form, fate, and function in human cardioids
Stefan M. Jahnel, Anna Dimitriadi, Julia Kodnar, Vasileios Gerakopoulos, Yajushi Khurana, Maximilian Mayrhauser, Tobias Ilmer, Keisuke Ishihara, Sasha Mendjan

Profibrotic Changes Following Tension Application in a Fetal Lamb Model of Long Gap Esophageal Atresia
Jessica C. Pollack, Nicolas Vinit, Shelley Jain, Rachel Conan, Melanie Bates, Mia Kwechin, Alicia Eubanks, Mike Xie, Amanda Muir, Emily Partridge

Ybx1 Deficiency Causes ROS-Driven IBD-Like Intestinal Inflammation and Postnatal Lethality
Bo Zhu, Lakhansing Pardeshi, Yingying Chen, Xianqing Zhou, Wei Ge

Dynamic Reorganization of Developmental to Adult Genome Topology Controls the Initiation and Stabilization of the Human Muscle Stem Cell State
Matthew A. Romero, Peggie Chien, Chiara Nicoletti, Hanna L. Liliom, Gabriella Cox, Emily Skuratovsky, Kholoud Saleh, Devin Gibbs, Lily Gane, Dieu-Huong Hoang, Luca Caputo, Jimmy Massenet, Débora R. Sobreira, Pier Lorenzo Puri, April D. Pyle

A single cell atlas defines perinatal factors that drive murine bone marrow development
Brian M Dulmovits, Carson Shalaby, Fangfang Song, James Garifallou, Joshua Bertels, Fanxin Long, Christopher S Thom

Aging disrupts tissue homeostasis and constrains blastema-mediated regeneration in the Cladonema medusa
Ren Kanehisa, Hiroko Nakatani, Sho Takatori, Taisuke Tomita, Masayuki Miura, Yu-ichiro Nakajima

Capturing self-renewing multipotent neural crest stem cells from human pluripotent stem cells
Yayoi Toyooka, Nami Kawaraichi, Daisuke Kamiya, Teruyoshi Yamashita, Yusaku Komoike, Kimiko Fukuda, Teppei Akaboshi, Hirokazu Matsumoto, Makoto Ikeya

Fibroblast-specific Deletion of Yap/Taz Impairs Mouse Postnatal Dermal Development by Suppressing Collagen Production and Deposition
Alexandre, Ava J Kim, Kirk C Hansen, Maxwell McCabe, Jun Young Kim, Zhaoping Qin, Zhaolin Zhang, Tianyuan He, Chunfang Guo, John J Voorhees, Gary J Fisher, Taihao Quan

Activation of developmental transcription factors using RNA technology promotes heart repair
Riley J. Leonard, Mason Sweat, Steven Eliason, William Kutschke, Brad A. Amendt

In vivo xenogenic reconstitution of human alveolar epithelial architecture and function
Akira Yamagata, Satoshi Konishi, Satoshi Ikeo, Hiroshi Moriyama, Senye Takahashi, Naoyuki Sone, Satoshi Hamada, Atsushi Saito, Takashi Kawaguchi, Shu Hisata, Akira Niwa, Toshiaki Kikuchi, Hirofumi Chiba, Megumu K. Saito, Koichi Hagiwara, Toyohiro Hirai, Mio Iwasaki, Takuya Yamamoto, Takeshi Takahashi, Shimpei Gotoh

PI3K inhibitor-free differentiation and maturation of human iPSC-derived arterial- and venous-like endothelial cells
Oliwia N. Mruk, Ralitsa R. Madsen

Scalable high-fidelity human vascularized cortical assembloids recapitulate neurovascular co-development and cell specialization
Shubhang Bhalla, Belda Gulsuyu, Damian Sanchez, Jayden M. Ross, Santhosh Arul, Adnan Gopinadhan, Muhammet Öztürk, Tanzila Mukhtar, Jonathan J. Augustin, Jerry C. Wang, Joseph Kim, Chang N. Kim, Sena Oten, Yohei Rosen, John M. Bernabei, Vijay Letchuman, Shantel Weinsheimer, Helen Kim, Elizabeth E. Crouch, Edward F. Chang, David Haussler, Mircea Teodorescu, Arnold R. Kriegstein, Tomasz J. Nowakowski, Ethan A. Winkler

Isthmin-1 is a Key Regulator of Induced Pluripotent Stem Cell–Derived Cardiomyocytes Maturation through Activation of p53 Signaling
Haowen Guo, Xin Zhou, Yang Shi, Bin Zhou, Jiaqi Tang, Faxiang Xu, Yanchen Guo, Fang Chen, Dongming Su, Qingguo Li

Cellular basis of accelerated whole-tooth regeneration
Talha Mubeen, Haowen He, George W. Gruenhagen, Anoushka Satoskar, Jeffrey T. Streelman

Identification of distinct functions of GLIS3 in β-cell generation critical to prevention of neonatal diabetes
David W. Scoville, Sara A. Grimm, Xin Xu, Benedict Anchang, Anton M. Jetten

Maternal lipids prime quiescent neural stem cells to reactivate in response to dietary nutrients
Md Ausrafuggaman Nahid, Susan E. Doyle, Kelly E. Dunham, Michelle L. Bland, Sarah E. Siegrist

Developmental programming of hematopoietic stem cell dormancy by evasion of Notch signaling
Patricia Herrero-Molinero, Eric Cantón, María Maqueda, Cristina Ruiz-Herguido, Arnau Iglesias, Jessica González, Brandon Hadland, Lluis Espinosa, Anna Bigas

Preconceptional immunomodulation partially corrects pregnancy abnormalities induced by endometriosis in a mouse model, with normalization of transcriptional alterations observed in the developing fetal-maternal interface at the single cell level
Kheira Bouzid, Roxane Bartkowski, Alix Silvert, Fabiana Moresi, Camille Souchet, Marine Thomas, Isabelle Lagoutte, Vaarany Karunanithy, Brigitte Izac, Charles Chapron, Pietro Santulli, Frédéric Batteux, Céline Mehats, Louis Marcellin, Ludivine Doridot

| Plant development

Novel repressors of cambium activity in Arabidopsis
Xing Wang, Jingyi Han, Emma K. Turley, Riikka Mäkilä, Anne-Maarit Bågman, Julia M. Kraus, Qing He, Hanan Alhowty, Joanna Edwards, Yuqi Li, Raluca Blasciuc, Wiktoria Fatz, Wenbin Wei, Miguel de Lucas, Siobhán M. Brady, Shixue Zheng, Chunli Chen, Ari Pekka Mäh-önen, J. Peter Etchells

AGP-Ca2+ binding is essential for pollen development and pollen tube growth in Arabidopsis thaliana
Jessy Silva, Maria João Ferreira, Paul Dupree, Matthew R. Tucker, Maria Manuela Ribeiro Costa, Sílvia Coimbra

Rewiring vascular patterning through translational control in Arabidopsis
Donghwi Ko, Raili Ruonala, Huili Liu, Ondrej Novak, Karin Ljung, Nuria De Diego, Robert Malinowski, Ykä Helariutta

Functional Redundancy of ZmSWEET6a/b in Mediating Sugar Transport and Redox Homeostasis for Maize Primexine Formation
Yan Zhang, Shuangtian Bi, Fengkun Sun, Jiajun Bu, Yurong Wang, Mateus Mondin, Zhaobin Dong, Weiwei Jin, Wei Huang

Arabidopsis GLK transcription factors interact with ABI4 to modulate cotyledon greening in light-exposed etiolated seedlings
Pengxin Yu, Friederike Saga, Miriam Bäumers, Ute Hoecker

Comprehensive characterisation of IAA inactivation pathways reveals the impact of glycosylation on auxin metabolism and plant development
Rubén Casanova-Sáez, Aleš Pěnčík, Federica Brunoni, Anita Ament, Pavel Hladík, Asta Žukauskaitė, Jan Šimura, Ute Voß, Ondřej Novák, Malcolm Bennett, Karin Ljung, Eduardo Mateo-Bonmatí

N-terminal phosphorylation inhibits Arabidopsis katanin and affects vegetative and reproductive development in opposite ways
Vivek Ambastha, Graham Burkart, Rachappa Balkunde, Ram Dixit

A conserved and predictable pluripotency window in callus unlocks efficient transformation in grasses and beyond
Yiyi Wang, Mengjiao Chu, Zhixia Wang, Jinhao Shao, Haijuan Zhang, Zhibiao Nan, Chunjie Li, Lei Lei

Two-step polar plastid migration via F-actin and microtubules ensures unequal inheritance during asymmetric division of Arabidopsis zygote
Keigo Tada, Hikari Matsumoto, Takao Oi, Zichen Kang, Tomonobu Nonoyama, Satoru Tsugawa, Yusuke Kimata, Shuhei Kusano, Shinya Hagihara, Shintaro Ichikawa, Yutaka Kodama, Minako Ueda

Ribosome profiling reveals distinct translational programs underlying Arabidopsis seed dormancy and germination
Maria Victoria Gomez Roldan, Elodie Layat, Julia Bailey-Serres, Jérémie Bazin, Christophe Bailly

Phosphovariants of the canonical heterotrimeric Gα protein, GPA1, differentially affect G protein activity and Arabidopsis development
David Chakravorty, Sarah M. Assmann

Wounding-Induced Redirection of Sugar Transport Fuels Tissue Repair
Rotem Matosevich, Mika Della Zuana, Itay Cohen, Idan Efroni

EARLY FLOWERING 3 (ELF3): a novel role in integrating environmental stimuli with root stem cell niche maintenance
Ali Eljebbawi, Rebecca C. Burkart, Laura Czempik, Vivien I. Strotmann, Xuelei Lai, Mark d. Tully, Luca Costa, Chloe Zubieta, Stephanie Hutin, Yvonne Stahl

The circadian clock gates lateral root development
Sota Nomoto, Allen Mamerto, Shiho Ueno, Akari E Maeda, Saori Kimura, Kosuke Mase, Ayano Kato, Takamasa Suzuki, Soichi Inagaki, Satomi Sakaoka, Norihito Nakamichi, Todd P. Michael, Hironaka Tsukagoshi

Initiation of asexual reproduction by the AP2/ERF gene GEMMIFER in Marchantia polymorpha
Go Takahashi, Saori Yamaya, Facundo Romani, Ignacy Bonter, Kimitsune Ishizaki, Masaki Shimamura, Tomohiro Kiyosue, Jim Haseloff, Yuki Hirakawa

A MICROTUBULE ASSOCIATED PROTEIN is required for division plane orientation during 3D-differential growth within a tissue
Zsófia Winter, Dorothee Stöckle, Takema Sasaki, Sophie Marc Martin, Yoshihisa Oda, Joop EM Vermeer

A shift in developmental allometry underlies the transition to a multi-ovulate strategy from a single-ovulate ancestral state in Phlox (Polemoniaceae)
Bridget Bickner, Elena M Kramer

Auxin coordinates cell states during Arabidopsis root development
Cassandra Maranas, Sydney VanGilder, Linda Nguyen, Jennifer Nemhauser

A chloroplast-localized protein AT4G33780 regulates Arabidopsis development and stress-associated responses
Zhengchao Yang, Zhiming Yu

Root-Suppressed Phenotype of Tomato Rs Mutant is Seemingly Related to Expression of Root-Meristem-Specific Sulfotransferases
Alka Kumari, Prateek Gupta, Parankusam Santisree, Injangbuanang Pamei, Satyavati Valluri, Kapil Sharma, Kavuri Venkateswara Rao, Shivani Shukla, Srilatha Nama, Yellamaraju Sreelakshmi, Rameshwar Sharma

| Environment, evolution and development

Programming of Embryonic Blood Brain Barrier and Neurovascular Transcriptome by an Anticipatory Acoustic Signal of Heat in the Zebra Finch
Prakrit Subba, Mylene M. Mariette, Katerina A. Palios, Michael G. Emmerson, Elisabetta Versace, Katherine L. Buchanan, David F. Clayton, Julia M. George

Developmental Hypoxia Increases Susceptibility to Cardiac Ventricular Arrhythmias in Adult Offspring
Mitchell C Lock, Kerri LM Smith, Aga Swiderska, Hayat Baba, Andrew Silverwood, Julia Dyba, Olga V Patey, Youguo Niu, Sage G Ford, Freja Steinke, Katherine Dibb, Andrew W Trafford, Dino A Giussani, Gina LJ Galli

Hypoxia couples growth and developmental timing by decoupling steroid synthesis and secretion
George P. Kapali, Alexander W. Shingleton

Early exposure to PFAS disrupts neuro-muscular development in zebrafish embryos
Zainab Afzal, Brian N. Papas, Vandana Veershetty, Evan E Pittman, Charles Hatcher, Jian-Liang Li, Warren Casey, Deepak Kumar

In vitro sexual dimorphism establishment in schistosomes
Remi Pichon, Magda E Lotkowska, Jude L. D. Bulathsinghalage, Madeleine McMath, Mary Evans, Benjamin J. Hulme, Kirsty Ambridge, Geetha Sankaranarayanan, Simon Kershenbaum, Sarah D. Davey, Josephine E. Forde-Thomas, Karl F. Hoffmann, Matthew Berriman, Gabriel Rinaldi

Maternal cardiometabolic dysfunction and fetal sex-specific alterations to uterine vascular reactivity in an ovine model of diet-induced obesity during pregnancy
Rachael C. Crew, Anna L.K. Cochrane, Youguo Niu, Sage G. Ford, Clement L.R. Cahen, Skaai H. Davison, Michael P. Murphy, Susan E. Ozanne, Dino A. Giussani

Prenatal Exposure to Bacterial Extracellular Vesicles Influences Fetal Gut Immunity and Immune Programming
Manuel S. Vidal, Ananth Kumar Kammala, Madhuri Tatiparthy, Ryan C. V. Lintao, Rahul Cherukuri, Ourlad Azeleus Tantengco, Shelly A. Buffington, Enkhtuya Radnaa, Lauren S. Richardson, Ramkumar Menon

Evolutionary dynamics of temporal transcription factor series in the insect optic lobe
Konstantina Filippopoulou, Elisavet Iliopoulou, Claire Julliot de La Morandière, Christy Lee, Marina Marcet-Houben, Toni Gabaldón, Jingyi Jessica Li, Nikolaos Konstantinides

lncRNAs contribute to caste differentiation as a regulatory layer in ants
Guo Ding, Fuqiang Lin, Jixuan Zheng, Dashuang Zuo, Zijun Xiong, Chenyan Liao, Bitao Qiu, Wenjiang Zhong, Jie Zhao, Weiwei Liu, Guojie Zhang

Sex chromosomes and sex hormones contribute jointly and independently to sex biases in cardiac development
Daniel F. Deegan, Gennaro Calendo, Priya Nigam, Raza Naqvi, Arthur P. Arnold, Nora Engel

Cell Biology

Mechanically competitive regulation of cell volume in cytoplasm-sharing cells connected by intercellular bridges
Hiroshi Koyama, Kanako Ikami, Lei Lei, Toshihiko Fujimori

Interface-Resolved Proteomics of Cell–Cell Membranes Reveals Early Spatial Polarity in a Vertebrate Embryo
Fei Zhou, Peter Nemes

Direct labeling of microtubule turnover reveals in-lattice repair and stabilization patterns in developing neurons
Ciarán Butler-Hallissey, Harrison M. York, Florence Pelletier, Jean-Marc Goaillard, Jérémie Gaillard, Manuel Théry, Pascal Verdier-Pinard, Christophe Leterrier

C. elegans E3 ubiquitin ligase EBAX-1 promotes non-apoptotic linker cell-type death through target-directed miRNA degradation
Lauren B. Horowitz, Olya Yarychkivska, Yun Lu, Shai Shaham

Kinesin-1 trans-synaptically regulates synaptic localization of SARM1 for asymmetric neuron diversification
Anaam Khalid, Peter Sahyouni, Jun Yang, Shengyao Yuan, Rui Xiong, Chiou-Fen Chuang

Lysosome-Related Organelles Orchestrate Guanine Crystal Formation in Pigment Cells
Anna Gorelick-Ashkenazi, Yuval Barzilay, Tali Lerer-Goldshtein, Tsviya Olender, Zohar Eyal, May Glaser, Yonatan Broder, Nadav Mishol, Rachael Deis, Merav Kedmi, Dvir Gur

P-glycoprotein exofection between fetal and maternal cells as a mechanism of intercellular material transfer at the feto maternal interface
Madhuri Tatiparthy, Amanda Wang, Vineeth Mahajan, Pilar Flores-Espinosa, Emmanuel Amabebe, Tilu Jain Thomas, Xiao-Ming Wang, Lauren S Richardson, Ramkumar Menon, Ananth K Kammala

Modelling

Clocks and Dominoes: Timing Mechanisms of Embryogenesis
Yonghyun Song, Brian D. Leahy, Hanspeter Pfister, Dalit Ben-Yosef, Daniel J. Needleman

Integrative Inference of Spatially Resolved Cell Lineage Trees using LineageMap
Xinhai Pan, Yiru Chen, Xiuwei Zhang

A proteomic signature of oocyte quality from models of varying oocyte developmental competence
Emily R. Frost, Dulama Richani, Anne Poljak, Ananya Vuyyuru, Xuihua Liao, Elise Georgiou, J M Binuri Gunasekara, Bettina P. Mihalas, Irene E. Sucquart, Kaushiki Kadam, Lindsay E. Wu, Robert B. Gilchrist

MorphoLearn: A morphology-driven workflow to decipher 3D electron microscopy segmentation in diatoms
Clarisse Uwizeye, Serena Flori, Jhoanell Angulo, Pierre-Henri Jouneau, Benoit Gallet, Pascal Albanese, Giovanni Finazzi

How simple physics drives the earliest stages of embryogenesisAlaina Cockerell, Peyman Shadmani, Krasimira Tsaneva-Atanasova, David M. Richards

Early multi-omic signatures and machine learning models predict cardiomyocyte differentiation efficiency and enable robust hPSC differentiation to cardiomyocytesAustin K. Feeney, Aaron D. Simmons, Elizabeth F. Bayne, Yanlong Zhu, Mason R. Pentes, Paulo F. Cobra, Jianhua Zhang, Timothy J. Kamp, Ying Ge, Sean P. Palecek

Tools & Resources

Establishing genetically controlled, closed colonies of an ascidian
The Ciona bio-resource consortium

HoloBio A Holographic Microscopy Tool for Quantitative Biological Analysis
Waira Mona, Maria J. Gil-Herrera, Emanuel Mazo, Daniel Córdoba, Sofia Obando, Maria J. Lopera, Rene Restrepo, Carlos Trujillo, Ana Doblas, Raul Castaneda

Array-CNCC: precise aggregation and arrayed plating facilitate quantitative phenotyping of human cranial neural crest cells and craniofacial disease modelling
Ewa Ozga, Katarzyna M Milto, Martina Demurtas, Lawrence E Bates, Graeme Grimes, Takuya Azami, Jing Su, Carlo De Angelis, Marco Trizzino, Jennifer Nichols, Hannah K Long

Sixteen isotropic 3D fluorescence live imaging datasets of Tribolium castaneum gastrulation
Franziska Krämer, Stefan Münster, Frederic Strobl

Integration of early-stage cryopreservation and cell cycle modulation into a flexible kidney organoid differentiation system
Xiaotian Yan, Jina Wang, Ming Xu, Chunlan Hu, Siyue Chen, Yufeng Zhao, Jiyan Wang, Ruiming Rong, Tongyu Zhu, Weitao Zhang

A robust human airway organoid platform enables scalable expansion and trajectory mapping of pulmonary neuroendocrine cells
Noah Candeli, Lisanne den Hartigh, Nicholas Hou, Andrés Marco, José Antonio Sánchez-Villacaña, Andrea Garcia-Gonzales, Shashank Gandhi, Francesca Sgualdino, Alyssa J. Miller, Jason Spence, Susana Chuva de Sousa Lopes, José L. McFaline-Figueroa, Hans Clevers, Talya L. Dayton

“All-in-one” Single-Cell Proteomic Analysis of Protein Alterations in Human Oocytes Undergoing in Vitro Aging
Jue Zhang, Yuting Lu, Shuoping Zhang, Xingyao Wang, Jiao Lei, Feitai Tang, Shen Zhang, Ge Lin

Real-time single-molecule imaging in zebrafish embryos uncovers non-canonical translation
Maëlle Bellec, Kenny Mattonet, Tatsuya Morisaki, Margaux Lay, Jie Liang, Damien Avinens, Vincent Martinet, Delphine Muriaux, Timothy J Stasevich, Jérémy Dufourt, Didier Y R Stainier

Integration of in situ hybridization and scRNA-seq data provides a 2D topographical map of the developing retina across species
Heer N. V. Joisher, ChangHee Lee, Chaitra Prabhakara, Isabella van der Weide, Yichen Si, Nicholas Lonfat, Constance Cepko

Research practice & education

Development and field test of an intervention to reduce conflict in faculty-doctoral student mentoring relationships
Trevor T. Tuma, Emily Q. Rosenzweig, Justin A. Lavner, Yichi Zhang, Erin L. Dolan

Collective AI use is associated with researcher engagement: Real-time evidence from a scientific conference
Hiroyuki Okada, Shigeto Seno, Ung-il Chung, Naganari Okura

The Demographic and GDP Impacts of Slowing Biological Aging
Raiany Romanni-Klein, Nathaniel Hendrix, Richard W. Evans, Jason DeBacker

Leveraging a hybrid cross-disciplinary training model to accelerate global bioinformatics capacity
Taras K. Oleksyk, Daryna Yakymenko, Sylwia Bożek, Viorel Munteanu, Wojciech Pilch, Zoia Comarova, Victor Gordeev, Grigore Boldirev, Dumitru Ciorbă, Viorel Bostan, Christopher E. Mason, Alexander G. Lucaci, Nadiia Kasianchuk, Daria Nishchenko, Victoria Popic, Andrei Lobiuc, Mihai Covasa, Martin Hölzer, Joanna Polanska, Alex Zelikovsky, Vasili Braga, Mihai Dimian, Paweł Łabaj, Serghei Mangul

Cloud-Connected Pluripotent Stem Cell Platform Enhances Scientific Identity in Underrepresented Students
Samira Vera-Choqqueccota, Drew Ehrlich, Vladimir Luna-Gomez, Sebastian Hernandez, Jesus Gonzalez-Ferrer, Hunter E. Schweiger, Kateryna Voitiuk, Yohei Rosen, Kivilcim Doganyigit, Isabel Cline, Rebecca Ward, Erika Yeh, Karen H. Miga, Barbara Des Rochers, Sri Kurniawan, David Haussler, Kristian López Vargas, Mircea Teodorescu, Mohammed A. Mostajo-Radji

Beyond Deficit and Coexistence: Modeling the Knowledge–Conspiracy–Mistrust Configuration in Public Understanding of Science
Ahmet Süerdem, Svetlomir Zdravkov, Martin J. Ivanov

Uncovering Conceptual Biases in DNA Stabilization: A Student-Led Investigation
Charlotte Polo, Ameeta Thandi, Olivia Chandler, Paula Lugert, Alyssa Hammoud, Theertha Madhi, Malena Ayala, A.J. Berrigan, Andrew Chen, Kate Gillett, Sohan Sanjeev, Mya Sareen, Sean Yu, Yang-yang Zuo, Shawn Xiong

Fine-Grained Detection of AI-Generated Writing in the Biomedical Literature
Richard She

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