In this quarterly webinar series, hosted by Pablo J. Sáez and Valeria Venturini, we aim to showcase the latest research in the field, and we’d love to hear from researchers (especially early-career researchers) who would like to present their work. You can get in touch with us at focalplane@biologists.com or fill in our short application form.

The post New FocalPlane features… webinar series on cell migration appeared first on the Node.

What makes IMEV special is not only its location on the French Riviera, but also the scientific history embedded in the place itself. Since the late 19th century, researchers have come to Villefranche-sur-Mer to study marine embryos, fertilization, plankton, and development; taking advantage of the bay’s extraordinary biodiversity and direct access to living marine organisms. In many ways, modern marine developmental biology was shaped in places like this.

And you still feel that spirit today.

Although IMEV is relatively small, it is well equipped and highly efficient for experimental work (Fig. 1).

Fig. 1. Institut de la Mer de Villefranche (IMEV), Villefranche-sur-Mer, France.

Access to marine organisms, imaging platforms, and technical support made it possible to rapidly test several GCaMP variants and explore live imaging approaches in sea urchin larvae (Fig. 2).

Fig. 2. Calcium imaging in the sea urchin larva Paracentrotus lividus expressing GCaMP6s.
A. Brightfield image overlaid with GCaMP6s fluorescence signal (green). B – B’. MAX projections from selected time windows of an XYT recording, showing representative spontaneous calcium activity patterns. Fluorescence intensity is displayed using the Fire (LUT).

A key part of what made this project so productive was being hosted by Luis Bezares Calderon.

I had never met Luis before this visit. I knew his work on neuronal and behavioral mechanisms in Platynereis, so during the proposal stage I sent him a simple email asking whether he would consider hosting me at IMEV. He generously agreed.

From the beginning, he gave me full access to his lab resources and created a highly collaborative environment. But what shaped the experience most was not only the experimental support, it was the way he constantly challenged the thinking behind the experiments.

His questions were persistent and fundamental: Why this approach? How will it answer your question? Are you just going to look at traces forever, or understand their function?

Coming from a molecular biology background, I was used to thinking mainly in terms of genes, molecules, and cellular mechanisms. Working with Luis pushed me to think more directly about nervous system function and behavior. For the first time in my life as a researcher, I witnessed behavioral assays in sea urchin larvae with my own eyes while simultaneously trying to connect activity dynamics to biological function.

This connection between molecules, cells, neural activity, and behavior is something Luis actively builds into his science. I found that intellectually challenging, stimulating, and genuinely exciting.

This experience reminded me that marine stations are not just places to run experiments. They are environments where questions can evolve quickly because of access, collaboration, and the intensity of scientific interaction.

I am very grateful to everyone at IMEV, and especially to Luis, for making this possible.

The post What can happen during a short stay at a marine station? appeared first on the Node.

Research laboratory presentation

The Marín-Juez laboratory, at the CHU Sainte-Justine Research Center, is recruiting PhD students and postdoctoral fellows (up to 5 years fully funded position).  Our laboratory is interested in the cellular and molecular mechanisms regulating cardiac regeneration and development.  

The successful applicant will join the Marín-Juez laboratory at the CHU Sainte-Justine Research Center, where they will have access to state-of-the-art facilities and technology platforms including Advanced imaging platform (light-sheet, spinning-disc confocal, multiphoton, STED super-resolution, etc.), genomics (DropSeq, 10x, Illumina Novaseq, Visium), IPSC Cell Reprograming and bioinformatics platforms.  The CHU Sainte-Justine Research Center provides a thriving scientific environment where the successful applicant will have the opportunity to work with multidisciplinary scientific teams and to collaborate with talented clinicians and researchers. 

Research project description

We have previously uncovered mechanisms governing coronary network replenishment, including the formation of a vascular scaffold that supports cardiomyocyte regeneration and mediates coronary-epicardial interactions and immune responses (Marín-Juez et al., PNAS 2016; Marín-Juez et al., Dev Cell 2019; El-Sammak et al., Circ Res 2022; Wang et al., Development 2024; Gupta et al. Dev Bio 2025; Rouf et al. Development 2026). Our recent work identifies the epicardium as a master regulator of cardiac fibrosis resolution and tissue replenishment (Kayman-Kürekçi et al. NCVR2026).

Building on these findings, we now aim to elucidate how the cardiac endothelium, epicardium, and immune system components cooperate to regulate tissue replenishment, as well as the specific mechanisms underlying their roles in cardiomyocyte regeneration and development.

PhD student position 

Applicants should have training in molecular biology, cell biology, or related fields.  Candidates should be enthusiastic about regenerative and developmental biology. Previous research experience with zebrafish and/or heart regeneration is highly valued but not essential. Candidates with experience in confocal/light-sheet imaging and/or genome engineering are strongly encouraged to apply.

Postdoc position 

We are looking for candidates with a Ph.D. in the biological sciences and laboratory experience in tissue repair/regeneration, cellular, molecular biology, or genetics.  Previous experience working with zebrafish, imaging and histology are highly valued but not essential. Candidates with experience in confocal/light-sheet imaging and/or genome engineering are strongly encouraged to apply.  Preference will be given to applicants with excellent collaborative and communication skills.  

How to apply

Candidates must send the required documentsto Rubén Marín Juez at ruben.marin.juez.hsj@ssss.gouv.qc.ca

Please provide:

• Curriculum vitæ
• Cover letter 
• References (2 or 3)

The post Molecular control of cardiac regeneration and repair appeared first on the Node.

Preprint highlights

• Canonical mTOR signaling supports complete fin regeneration selected by Theodora M Stougiannou
• Whole-Cell Proteomics Identifies Novel Regulators of Ciliogenesis Beyond the Axoneme selected by Jawdat Sandakly
• Synthetic lumen rounding directs neural progenitor division mode selected by Sristilekha Nath
• Lamin A/C directs nucleosome-scale chromatin remodeling to define early lineage segregation in mammals selected by Deevitha Balasubramanian
• Abnormal ventricular wall patterning precedes and drives MYBPC3 hypertrophic cardiomyopathy selected by Theodora M Stougiannou

Preprint:

Theodora M Stougiannou

Preprint:

Canonical mTOR signaling supports complete fin regeneration
Josane F. de Sousa, Gabriela Lima, Louise Perez, Michaela Tsanova, Cyrus Bronson, Garrison Boehl, Icyss Sargeant, Rogerio Gomes, Aline C. Dragalzew, Wainna B. Mendes, Igor Schneider

preLight:

Fins, and cells, and signals, and regeneration, oh my! How the Senegal bichir regrows its fins after amputation.

The authors of this preprint investigate fin regeneration in the Senegal bichir (Polypterus senegalus), a type of ray-finned fish capable of full fin regeneration; this biological characteristic is quite impressive on its own, as the fin includes different tissues, such as skeletal, cartilaginous, muscular and connective tissue with complexity comparable to that found in tetrapod limbs.

The preprint authors show that regeneration entails the activation of canonical mTOR cellular programs, as treatment with the mTOR inhibitor rapamycin prevented this regeneration, though wound healing proceeded normally. Signaling was activated upon amputation, first in epithelial cells in the epidermis and then in adjacent mesenchymal cells below the superficial layers, as well as myeloid cell types.

It seems that mTOR programs in myeloid populations are responsible for the coordination of regenerative procedures across different cell types, as well as its eventual resolution.  Moreover, existence of such programs in species of fish highlights the ‘ancient’ evolutionary origins of tissue regeneration, giving hope for application of these principles in other species.

Jawdat Sandakly

Preprint:

Whole-Cell Proteomics Identifies Novel Regulators of Ciliogenesis Beyond the Axoneme
Xiaolu Xu, Yanbao Yu, Tony Zheng, Fiona Clark, Jean Ross, Neha Sindhu, Andre L P Tavares, John B Wallingford, Shuo Wei, Jian Sun

preLight:

Uncovering new players in ciliogenesis by whole-cell proteomics

Motile cilia are microtubule-based organelles that are involved in fundamental biological processes such as embryonic development, signalling, and mucus clearance. Their dysfunction results in several disorders known as ciliopathies.

Several efforts over the years have helped in elucidating the molecular architecture of motile cilia and in understanding ciliary structures and functions. Moreover, previous proteomic studies provided valuable insights into the axonemal composition. However, many molecular regulators of ciliogenesis remain unknown and other critical cellular components beyond the axoneme involved in ciliogenesis require further investigation.

In this preprint, the authors performed a high-resolution whole-cell proteomic profile of multiciliated cells (MCC), whose function is regulated by axonemal proteins, basal bodies, cytoplasmic factors, and nuclear components. They induced MCC cell fate in Xenopus, therefore enriching ciliary proteins and generating mucociliary organoids. Following their high-depth proteomic profiling, they identified several previously uncharacterized proteins that are essential for MCC maintenance and ciliogenesis. Through in situ hybridization, immunostaining, and gene knockdown, they further confirmed the new candidates, thus providing new potential targets to be further explored to gain a better understanding of the mechanisms related to ciliopathies.

Sristilekha Nath

Preprint:

Synthetic lumen rounding directs neural progenitor division mode
Marina Marchenko, Guillermo Martínez Ara, Juslina Pulikkal, Keisuke Ishihara, Miki Ebisuya

preLight:

Cells read lumen geometry to instruct division mode and lineage progression

During early brain development, tissue geometry – including lumen geometry – dynamically changes; a process which varies across species. But does this geometry simply result from development, or does it actively instruct how cells behave?

The authors of this preprint investigate this question by artificially controlling lumen geometry in brain organoids using two approaches: chemical induction of Shroom3, a protein that drives apical constriction and OptoShroom3, an optogenetic system enabling precise, light-controlled activation. The latter enables spatially targeted control without affecting overall Shroom3 levels within the organoids.

The results reveal that lumen geometry is not a passive consequence of development, but an active regulator of cell behavior. Chemically-induced Shroom3 organoids formed much rounder lumens and neural buds, and generated basal progenitor cells faster than controls, while cells gradually switched from vertical to horizontal cleavage planes over time, a critical reorientation since horizontal division results in asymmetric cell division that generates more basal progenitors, whereas vertical division (in controls) maintains more apical progenitors. When the authors used OptoShroom3 to create rounded lumens with localized blue light illumination, apical progenitor cells in target buds similarly shifted toward horizontal cleavage planes within an hour, whereas those without illumination (control bud within the same organoid) did not.

These findings demonstrate that cells ‘read’ their geometric environment to make developmental decisions, suggesting lumen shape as a key determinant, not merely a consequence of morphogenetic outcomes, a principle likely applicable broadly across organs and species. 

Deevitha Balasubramanian

Preprint:

Lamin A/C directs nucleosome-scale chromatin remodeling to define early lineage segregation in mammals
Alice Sherrard, Liangwen Zhong, Caroline Hoppe, Srikar Krishna, Scott Youlten, Curtis W. Boswell, Stephen Cross, Fiona E. Sievers, Goli Ardestani, Denny Sakkas, Liyun Miao, Zachary D. Smith, Berna Sozen, Antonio J. Giraldez

preLight:

Nuclear lamins direct the first lineage decision in mammalian cells

The first lineage decision during mammalian development into the inner cell mass (ICM) and trophectoderm (TE) is well known to be initiated by transcriptional and epigenetic factors and reinforced by mechanical forces. While global chromatin organization is understood to be important for this process, it remains unclear how the fine-scale distribution of chromatin and nucleosomes plays a role in these cell fate decisions.

To investigate this, the authors set up an improved chromatin electron tomography protocol called ChromEMT to observe nanometer-scale sub-nucleosomal structures. Using ChromEMT on human and mouse cell cultures before, during, and after specification into ICM and TE, they identified key differences in chromatin packing density and nucleosome spacing between these lineages. Importantly, they found that TE nuclei have highly compacted chromatin at their nuclear periphery. In line with this increased peripheral compaction, the authors could show that proteins located at the inner nuclear membrane, particularly lamins A and C, are specifically upregulated in TE cells across mammals. Loss of Lamin A/C resulted in loss of peripheral chromatin compaction and upregulation of pluripotency genes in TE cells, suggesting an overall transition to ICM-like characteristics. This, in turn, impairs normal progression through embryogenesis.

In concert with many more supporting findings, this preprint demonstrates how chromatin compaction and nuclear lamins directly shape early mammalian development.

Theodora M Stougiannou

Preprint:

Abnormal ventricular wall patterning precedes and drives MYBPC3 hypertrophic cardiomyopathy
Alejandro Salguero-Jiménez, Alba Pau-Navalón, Marcos Siguero-Álvarez, Carlos Relaño-Rupérez, Javier Santos-Cantador, María Sabater-Molina, Xiaoxi Luo, Laura Lalaguna, Laura Sen-Martín, Daniel Martín Pérez, Abel Galicia Martín, Bin Zhou, Juan Antonio Bernal Rodríguez, Fátima Sánchez-Cabo, Enrique Lara-Pezzi, Jorge Alegre-Cebollada, Juan R. Gimeno-Blanes, Donal MacGrogan, José Luis de la Pompa

preLight:

More ‘heart’, more problems; a natural history of myocardial hypertrophy progression from embryonic development to adulthood and the role of sarcomeric protein mutations (Mybpc3) in its emergence.

The authors of this preprint investigated the developmental biology underlying hypertrophic cardiomyopathy and left ventricular non-compaction in mice. To this end, they used CRISPR-Cas9, a method used to induce genetic alterations, to introduce MYBPC3 frameshift mutations in the mouse genome and then followed these mice from embryonic and fetal development into adulthood. Adult mice with these mutations displayed hypertrophic cardiomyopathy but with no evidence of left ventricular non-compaction, as opposed to humans. These formations began as trabecular enlargement and crypt enlargement during embryonic development and progressed to hypertrophy in adulthood. Lineage tracing studies further showed invasion of cardiomyocytes normally found in compact myocardium (Hey+ cardiomyocytes), into the developing trabeculae, while after birth, Hey+ cardiomyocytes became restricted to compact myocardium and the inner trabecular myocardium underwent hypertrophy. This is associated with downregulation of the Prdm16; this study highlights how the latter has potential to combat myocardial hypertrophy.

This study highlights the natural history of myocardial hypertrophy and how loss of Mybpc3 is associated with reduction in Prdm16 and onset of pathological hypertrophic remodeling.

The post preLighters’ choice – A curated selection of recent preprints appeared first on the Node.

The Medical Research Council provided £750K in funding for the National Mouse Genetics Network’s Business Engagement Fund with a call for applications in early 2023. The Business Engagement Fund supported 3–12-month projects, providing grants of £15–100K, with the expectation that matched funding would be provided by industry collaborators. Funded projects were designed to build and strengthen collaborations between the Network and businesses through feasibility, pilot, or initial studies. These activities aimed to explore ideas and generate initial data to support the development of competitive collaborative grant proposals.

We are now reporting on the first of these projects, highlighting how collaborative endeavours of this kind can help shape preclinical research and accelerate the development of therapeutic interventions.

The project was a partnership between Professor Nick Greene of University College London and OutFox Bio. Nick is a member of the Congenital Anomalies Cluster and a leading academic researcher studying a range of birth defects, with a long-standing interest in the role of folates in development and inherited metabolic disease. OutFox Bio is a delivery technology company focused on the development and optimisation of next-generation lipid nanoparticle (LNP) gene delivery technologies, designed to enable new gene therapy approaches and expand their potential applications.

A life-limiting incurable disease

Non-Ketotic Hyperglycinemia (NKH) is a life-limiting autosomal recessive neurometabolic disease that presents in neonates with lethargy, hypotonia, myoclonic jerks and apnoea. Affected children experience profound neurological impairment and complex epilepsy. Around one-third of infants with severe neonatal-onset NKH die within the first year, but age at death is highly variable, with some children surviving into their teenage years.

NKH is caused by mutations in genes that encode the glycine cleavage system (GCS). Most patients (80%) carry mutations in GLDC (glycine decarboxylase), with the remainder carrying mutations in AMT (aminomethyltransferase). The GCS decarboxylates glycine, with the concomitant transfer of a one-carbon (1C) group to tetrahydrofolate (THF), generating methylene-THF. Subsequent reactions in folate one-carbon metabolism (FOCM) provide 1C groups for multiple outputs, including nucleotide biosynthesis and methylation reactions. Hence, GCS dysfunction leads both to the accumulation of excess glycine in the body and to suppression of FOCM.

There is no cure for NKH; current treatments have limited efficacy. Prognosis remains very poor, highlighting an urgent unmet need for novel therapies. There is currently no established standard of care for NKH, although patients are typically treated with multiple anti-seizure medications. The most common treatment is sodium benzoate, which is administered to lower circulating glycine by stimulating glycine conjugation in the liver, generating hippurate (benzoylglycine) for excretion. Benzoate helps with seizure control but can be toxic and is associated with severe gastrointestinal side effects, necessitating long-term co-administration of proton-pump inhibitors which may carry additional risks. Replacement of benzoate has been highlighted as a priority during discussions with families of affected children.

To investigate NKH pathogenesis and develop novel treatments, Nick’s group developed a GLDC-deficient mouse model that recapitulates hallmark features of the disease, including elevated plasma and tissue glycine and neurological abnormalities. Loss of glycine cleavage system activity was confirmed by enzymatic assay and metabolic tracing using isotopically labelled glycine. Glycine is both a biomarker and a therapeutic target in NKH; both glycine and guanidinoacetate, a glycine–arginine conjugate, are epileptogenic.

In GLDC-deficient mice, the group observed that liver-specific reinstatement of GLDC expression or stimulation of hepatic glycine conjugation through benzoate administration led to normalisation of liver tissue glycine and glycine derivatives, correction of blood glycine concentrations, and reduction of glycine levels in the brain, the main site of NKH pathogenesis. These studies provide proof of principle for liver-directed therapy as a means of controlling systemic and brain glycine levels.

The causative genes are known, making NKH potentially amenable to therapies that restore gene expression. The aim is to develop RNA-based approaches to reinstate GLDC expression and normalise metabolism in NKH. Lipid nanoparticle (LNP)-mediated delivery of mRNA to the liver represents an attractive methodology for therapeutic gene expression. LNP systems have proven to be effective and safe for mRNA delivery and are already in clinical use for other conditions.

In this NMGN Business Engagement Fund project, undertaken in partnership with OutFox Bio, the team initially sought to address two key questions using a reporter-encoding mRNA.  First, they tested whether the liver in NKH remains amenable to LNP-mediated mRNA delivery despite abnormal metabolism. Second, they sought to identify the optimal LNP composition for mRNA delivery to the liver in the NKH GLDC-deficient mouse model. The team identified LNP compositions with improved efficacy compared with clinically approved benchmarks. They also confirmed that compromised glycine metabolism in the liver does not hinder uptake or expression of LNP-delivered mRNA in the NKH mouse model. For example, expression of LNP-mediated reporter expression was at least as high in GLDC-deficient mice as in wild-type mice following treatment with each LNP composition. These findings provided the proof of concept for extending the project to therapeutic mRNA, prioritising the lead LNPs.

The ongoing objective of the project is to develop an mRNA-based therapy that reinstates liver GLDC expression, normalises metabolism, and improves neurological outcomes in the GLDC-deficient NKH mouse model. Outputs from this project are expected to provide an evidence base for advancing this approach towards clinical trials in children with NKH.

Nick presented some of this work at the NKH Crusaders 11th Annual International Family Conference in Boston, where he gave a presentation and took part in several round-table discussions. The event was reported on social media, where Nick’s talk was also mentioned.

The post A Business Engagement Fund project to test Lipid Nanoparticle (LNP)-mediated mRNA delivery and efficacy appeared first on the Node.

Although Marianne and I first met at the Aspen Center for Physics, USA, in the summer of 2022, our scientific careers had, in a sense, been pre-slated to converge.

Marianne began her research life as a statistical physicist, studying the dynamics of ultracold gases (about as far from developmental biology as you can get). But she followed her curiosity steadily toward living systems. This path eventually landed her in Bill Bialek’s group at Princeton University, USA, one of the world’s great incubators for quantitative approaches to biology. I never overlapped with her there, but I spent my own PhD and postdoc years in Princeton’s Molecular Biology Department, orbiting the same weekly biophysics seminar, the kind of room that trains you to ask the most fundamental version of your question and hold out for a real answer. We started our independent labs at similar times, I at UC Santa Barbara, USA, and Marianne, a few years later, at TU Delft, The Netherlands. Whether by attraction to the same scientific community or simply by the shared foundation of our training, I suspect it was only a matter of time before we found each other.

But it took a mountain, or nearly.

Twining Peak, Colorado. Elevation 13,711 feet.

One of the best-kept secrets of the Aspen Center for Physics is its mandatory downtime. Every couple of days, the entire group stops working and goes hiking. No laptops, no slides. Just altitude, views of the Rockies, and several hours of unstructured conversation. I am convinced that some combination of thin air and long trails produces a particular quality of scientific thinking that is very hard to replicate in a seminar room or at a desk.

My lab had just engineered a new suite of optogenetic tools that allowed precise, programmable control of developmental signaling pathways in human embryonic stem cells, including, critically, the Wnt pathway, which governs cell fate decisions and patterning in early development and adult tissues. Borrowed from neuroscience and adapted for developmental biology, optogenetics allows you to use light to activate specific signals in cells with millisecond precision. For the first time, we could systematically interrogate how cells respond to signals delivered at different rhythms and timescales. I was looking for theorists who wanted to think about these questions seriously.

I found one on a mountain.

Twining Peak sits directly on the Continental Divide, where precipitation from the same slopes drains west toward the Roaring Fork River and east toward Arkansas. On this mountain, the same water flows in two different directions at once. The trail is not a casual outing, and the terrain is unforgiving at altitude. Somewhere on that mountain, probably on the way back down, we sketched the outlines of several project ideas and agreed to stay in touch. It felt inevitable.

“If I didn’t know this was biology, I’d almost say you’ve hit it at a resonance or something.”

What happened next was mostly emails and Zoom calls, which is to say, the unglamorous reality of international collaboration. Marianne is in Delft; I am in Santa Barbara. The overlap in our waking hours was narrow, so most of our early meetings required one of us to be awake either very early or very late. Reading back through our correspondence from late 2022, I am struck by how many messages were essentially calendar negotiations: a wedding, a conference, a colloquium that ran long, nine emails to lock in a single Zoom meeting. When we did finally connect, our calls would often be interrupted by a Dutch overhead announcement that rang through Marianne’s building at 5 or 6 PM, a recorded voice cheerfully informing everyone that the working day was over and it was time to go home. It became a kind of running joke.

But those early conversations were productive and fun. Marianne took on a graduate student, Olivier Witteveen, to work on the theory side. I began sending data. And it was during one of these data-sharing exchanges, in March 2023, that the concept at the heart of our paper was first named.

I had shared a set of experiments tracking how beta-catenin, the central transcription factor of Wnt signaling, responded to optogenetic inputs of varying durations and intensities into the upstream receptor system. The traces were very complex. Peaks where you expected them, but also puzzling dips, and an intriguing non-monotonic response to signals delivered at certain intervals. Marianne, looking at the data with fresh eyes and a physicist’s intuition, wrote back, “If I didn’t know this was biology, I’d almost say you’ve hit it at a resonance or something.”

I replied: “I was thinking resonance as well!”

As it turned out, the cells were doing the opposite. Anti-resonance is not where the response peaks but where it vanishes. We had identified the right concept and the wrong sign, a class of error that is actually quite common in physics.

Resonance and anti-resonance are well-developed concepts in physics and engineering. Resonance is when a system responds especially strongly to inputs delivered at a particular frequency. It’s why bridges can shake apart in the wind, why wine glasses shatter at the right musical note. Anti-resonance is the counterpart. A frequency at which the system’s response drops out almost entirely, even as neighboring frequencies drive a strong reaction.

These ideas apply to any physical system that receives inputs that vary in time, and yet they have rarely been applied to biology. The reason, until recently, was that you could not watch the signaling dynamics in a living developmental system over the timescales needed, and you certainly couldn’t deliver precisely timed, reproducible signals to probe them. The convergence of low-phototoxicity long-term imaging and cellular optogenetic tools changed both of these things at around the same time. So with these new tools, we could treat a developing tissue in the same way engineers have studied circuits for almost a century: put in a signal, vary its frequency, and measure how the system responds.

What we found in the Wnt pathway is that cells do not respond uniformly across frequencies. At certain input rhythms, the transcriptional response (the downstream readout of whether a cell “heard” the signal) falls to near zero, even as nearby slower or faster signals drive robust activation. The system has a blind spot. That is anti-resonance.

The deeper question, why, is one we speculate about but do not claim to have answered. Is anti-resonance a developmental gatekeeper that helps cells avoid dangerous intermediate identities by filtering out certain signal dynamics? Is it an anti-cancer mechanism that blocks runaway activation by particular signaling patterns? Or is it a spandrel in the Gould-and-Lewontin sense, an architectural byproduct with no function of its own, present simply because of how the underlying circuit is built? We genuinely don’t know. What we do know is that we are very early in our understanding of developmental signaling as a temporal phenomenon, and that most of what we currently call a signaling pathway is really a static snapshot of something that is fundamentally dynamic.

I visited Marianne in Delft in the spring of 2024; she came to present at our departmental seminar at UCSB that fall. In between, we had monthly meetings, almost always early morning Pacific time, which is early evening in the Netherlands, punctuated by that cheerful Dutch announcement that the working day was officially over.

We were both Assistant Professors, both trying to build our labs, find funding, attract students, figure out how to operate independently, and both aware that the tenure clock does not pause for transatlantic collaborations. But the calls were genuinely fun, which I had not counted on. We never had to convince each other that the questions were interesting, which turns out to be more than half the battle. And when an experiment did not match what the theory predicted, or a theoretical prediction seemed to have nothing to do with what the cells were actually doing, we could say so without it becoming a diplomatic incident. That back-and-forth, a physicist poking holes in the biology and a biologist pushing back on the theory, is where most of the real ideas in this paper came from.

The most satisfying moment of this project had nothing to do with the science directly. At some point in the past year, I tried to schedule a meeting with my graduate student Sam Rosen, who is co-first author on the eLife paper. He couldn’t make it. He was on a scheduled call with Olivier Witteveen, Marianne’s student and his co-first author, a call they had set up themselves, independently, without prompting from either of us.

They had built their own version of what Marianne and I had built. We are still figuring out why cells ignore certain signals. Apparently, our students were paying attention to different signals entirely.

The eLife paper can be found at https://elifesciences.org/articles/107794. The companion Physics Review Research paper is at https://journals.aps.org/prresearch/abstract/10.1103/f7qj-f7qy.

The post The signal that got through appeared first on the Node.

The dot plots are often accompanied by a graphical statistical summary. Common statistics are the mean or median. A more comprehensive statistical summary is provided by the box plot. The box plot was first proposed by Mary Eleanor Spear in her book “Charting Statistics” and publicised by the work of John W. Tukey. Open source tools, such as the user-friendly web tool BoxPlotR, have contributed to a wider adoption of box plots in publications. The box plot is characterised by 5 values, the median, the two borders of the box that indicate the IQR, and two whiskers. The whiskers can reflect multiple things, but most commonly indicate the most extreme data-point that is maximally at 1.5 x IQR from the border of the box (Krzywinski & Altman, 2014).

Since a box plot summarises the data distribution with 5 values, it does not add any information when the data consists of only 5 or less points. This can also be seen in figure 2 for conditions B & D. Adding a box plot to a condition that has only 5 datapoints would be similar to adding the mean for only 1 datapoint. Since some datasets have variable numbers of observations per condition, it would be ideal to only display the box plot when sufficient observations (n>5) are present. To do this in R with {ggplot2}, I considered defining a new geom (if you are interested in that, I recommend this tutorial), but then I realized that it can be done by filtering the data within the box plot function (inspired by the work of June Choe on {ggtrace}, see also this video: https://youtu.be/dUBnitXf5mk).

The trick is to use a filter() function within the geom_boxplot() definition to keep only the conditions for which n>5 (aggregated for each condition by group_by(group)). Here’s the R code:

#Filtered box plot, only drawing a box for conditions that have n > 5
ggplot(demo_data, aes(x = group, y = value, fill = group)) +
  geom_boxplot(
    data = ~ .x %>% group_by(group) %>% filter(n() > 5)
  ) +
  geom_jitter(width = 0.2, size = 1.5) +
  theme_classic() +
  theme(legend.position = "none")

The resulting plot only shows a box plot when n>5:

The use of a filter() function within the definition of a geom is an elegant method for getting rid of the box plot when the number of observations is too low. In general, this approach is very powerful and gives more control over plotting with ggplot2. There’s probably a ton of other applications, and one that comes to mind is to filter data based on some criterion and changing the color, e.g. for outliers. And, fun fact, Figure 1 was also created using the filter() function. Check out the R code (for all plots) here: https://github.com/JoachimGoedhart/Unboxing-data

The post Unboxing data appeared first on the Node.

Written by David Kelly

Researchers at the University of Colorado Anschutz may have identified why many cancer patients say food suddenly tastes unpleasant during treatment.

The study, published today in Development, found that a class of targeted cancer drugs known as tyrosine kinase inhibitors (TKIs) can change how taste buds are maintained—reducing the ability to taste sweet foods and altering flavor perception overall. While the study was conducted in animal models, researchers believe similar changes likely occur in humans.

The findings offer the clearest explanation to date for a common but often overlooked side effect of cancer treatment.

What researchers found

Using mouse models and lab-grown taste tissue, scientists studied the cancer drug cabozantinib and discovered:

• Taste buds as a whole were not damaged or reduced in number by drug treatment
• The composition of cells inside taste buds shifted
• Drug treatment reduced the number of cells that detect sweet tastes
• Drug treatment increased the number of cells that detect bitter and savory (umami) tastes
• Mice lost their preference for sweet-tasting solutions

Researchers identified an unexpected cause: a protein called KIT.

While TKIs are used to block cancer growth pathways, they also unintentionally block KIT—an important regulator of taste cell development.

When KIT is blocked:

• Sweet-sensing cells fail to develop properly
• Bitter/umami-sensing taste cells take their place

The proportion of sweet and bitter taste bud cells is very tightly controlled. When this proportion is altered, taste perception may drastically change.

“If you lose the sweet component of everything you eat, your entire sense of taste becomes distorted,” said senior author Linda Barlow, PhD, professor of cell and developmental biology at CU Anschutz.

Why it matters

TKIs are important anti-cancer drugs that significantly extend survival in several types of advanced cancers. However, an estimated 10% to 50% of patients taking these drugs experience taste changes, known as dysgeusia.

Though often considered minor, the impact of dysgeusia can be significant:

• Loss of appetite
• Weight loss
• Poor nutrition
• Social withdrawal and reduced quality of life

“It’s difficult for them to enjoy a meal with their family and friends,” Barlow said. “Nothing tastes good to them so they withdraw and become isolated. Isolation leads to depression.”

Study co-author Elaine Lam, MD, professor of medicine and medical oncology at the CU Anschutz Cancer Center, said the drugs are meant to block blood vessels developing in tumors, effectively starving them. Unfortunately, they also cause unintended consequences.

“People don’t eat and they lose weight. This sometimes leads us to reduce or interrupt the dose of their drugs,” said Lam, a kidney cancer specialist. “This research is important because it identifies the underlying mechanisms that affect taste. Now we have to figure out the best way to treat this.”

Lam said possible solutions include designing cancer drugs that avoid blocking KIT or developing treatments to protect taste function.

What’s next

Future research will focus on confirming these findings in patients and identifying ways to prevent or reduce taste changes.

Bottom line

Targeted cancer drugs called tyrosine kinase inhibitors may not destroy taste buds—but they can change their cellular makeup, shifting the balance away from sweet-sensing cells and potentially changing how food tastes.

The study in Development is titled “Tyrosine kinase inhibitors affect sweet taste and dysregulate fate selection of specific taste bud cell subtypes via KIT inhibition”. The lead author is Christina M. Piarowski, PhD. Additional authors are Jennifer K. Scott, Courtney E. Wilson, PhD, Heber I. Lara, PhD, Ernesto Salcedo, PhD, Andrew S. Han, Peter J. Dempsey, PhD and Jakob von Moltke, PhD. The study is available here. 

This press release was originally published on the University of Colorado Anschutz news page.

The post Some Cancer Drugs Disrupt Taste by Changing the Cells Inside Taste Buds, Study Shows appeared first on the Node.

MorphoNet 2.0: breaking the glass ceiling of 3D+time bioimage curation

Modern microscopy now allows us to image living systems in three dimensions over time. From early embryonic divisions to complex tissue morphogenesis, we can follow every cell with remarkable spatial and temporal resolution.

Yet a critical bottleneck remains. Even with powerful AI-based segmentation tools, such as Cellpose or StarDist, errors are inevitable. In large 3D+time (3D+t) datasets, a seemingly small error rate quickly translates into thousands of mis-segmented cells, broken lineages, and ultimately misleading biological conclusions.

Correcting these errors — a process known as curation — is often so time-consuming that it becomes the real “glass ceiling” of quantitative developmental biology.

MorphoNet 2.0 was designed to break this glass ceiling.

From segmentation to curation

MorphoNet 2.0 is a major evolution of the original platform. Rather than proposing yet another segmentation algorithm, it focuses on a problem that is just as critical, but far less addressed: how to efficiently assess, correct, and validate 3D and 4D segmentations at scale.

The key idea is simple: automated segmentation is only useful if biologists can trust and refine its output. MorphoNet 2.0 makes this process fast, efficient, interactive, and accessible to non-programmers.

A hybrid architecture built for 3D+t data

Unlike the original web-based MorphoNet, version 2.0 is a standalone application designed to run on standard research workstations and laptops. Its architecture deliberately bridges two complementary worlds:

• a high-performance 3D interface, powered by the Unity game engine, for smooth exploration of thousands of objects in real time

• a Python backend integrated with powerful scientific image-processing libraries, enabling advanced image analysis, editing, and AI-based segmentation directly on raw data.

Letting the data highlight its own problems

A major challenge in large 3D datasets is simply knowing where to look. Manually inspecting every cell is unrealistic.

MorphoNet 2.0 addresses this by computing unsupervised quality metrics for each segmented object, such as volume, shape regularity, boundary intensity, or temporal stability. These properties are projected, as color maps, directly onto the 3D meshes or cell lineages, turning potential errors into visible outliers.

While these metrics are not absolute measures of correctness, they act as intelligent guides, directing experts toward regions that deserve closer inspection.

Curation as a local, interactive process

Once a potential error is identified, MorphoNet 2.0 enables rapid local, targeted corrections rather than global reprocessing.

This is achieved through a modular plug-in system that allows users to perform a wide range of operations, including (but not limited to):

• re-segment specific regions using AI or classical methods
  

• split fused cells or merge over-segmented fragments

• remove specifically small objects
  

• propagate corrections across time in 3D+t datasets
  

• edit and repair cell lineages.

Because plug-ins operate only on user-defined regions of interest, most corrections take seconds rather than hours. This transforms curation from a batch process into a true human-in-the-loop workflow, with immediate visual feedback.

MorphoNet 2.0 provides an open plug-in architecture that enables contributors to develop and share custom plug-ins tailored to their own segmentation, tracking, or lineage-repair challenges.

Tested on real biological datasets

This paper demonstrates MorphoNet 2.0’s successful use on five previously published datasets spanning insects, plants, nematodes, echinoderms, and ascidians. These case studies show how the platform can:

• reveal hidden segmentation errors in datasets long considered “finished”
  

• significantly improve segmentation quality through iterative correction
  

• polish cell lineages to a level suitable for studying subtle biological variability.
  

In one example, rare lineage errors scattered across thousands of cells were identified and corrected in a few hours — even in datasets comprising several thousand segmented objects — a task that would have been practically infeasible with traditional tools.

Why this matters for AI and data reuse

High-quality 3D ground truth data are essential for training and benchmarking modern AI models. Yet producing such datasets is extremely costly when curation tools do not scale.

By making 3D+t curation feasible, traceable, and accessible to biologists, MorphoNet 2.0 directly addresses this gap. It helps turn raw automated outputs (“silver truth”) into reliable, reusable datasets (“gold truth”) that can support reproducible analysis, fair algorithm comparison, and community challenges.

Who is MorphoNet 2.0 for?

MorphoNet 2.0 is designed for:

• developmental and cell biologists working with complex 3D or 4D imaging data

• imaging facilities producing reference datasets
  

• researchers developing or benchmarking segmentation and tracking algorithms.
  

No programming skills are required to use the platform, but its open, Python-based plug-in architecture allows advanced users to extend it and share new tools with the community.

Looking ahead

MorphoNet 2.0 positions complex 3D curation as a fast and manageable activity rather than a never-ending technical burden. By combining intuitive 3D interaction, unsupervised quality assessment, and local correction, it offers a practical solution to one of the most persistent bottlenecks in modern bioimage analysis.

Find out more

Read the paper


Download MorphoNet 2.0


Documentation & use cases

The post Featured resource: MorphoNet 2.0 appeared first on the Node.

I have long been interested in understanding how development emerges at the intersection of molecular and spatial organization. On one hand, decades of work have identified the genes and signaling pathways that control cell fate. On the other hand, classical embryology and biophysics have revealed how cells move, change shape, and assemble into tissues. Yet, directly connecting these two layers—linking gene expression to spatial patterns and morphogenesis at the scale of a whole embryo—has remained challenging.

When I joined the Schier lab in March 2020, single-cell RNA-seq approaches had already enabled the reconstruction of developmental trajectories with remarkable molecular detail [1]. For the first time, we could computationally line up cells along developmental paths and begin to understand how cell fates emerge at the whole-embryo level. But something always felt missing to me: these trajectories existed in abstract space, detached from the physical embryo. We could describe where cells were going, but not where they actually were.

At the same time, there was growing interest in spatial transcriptomics within the lab and through our membership in the Allen Discovery Center for Cell Lineage Tracing. This created an opportunity to bridge molecular and spatial information in developing systems. With my background in imaging and technology development, I was particularly drawn to the idea of building a method that could map gene expression across whole embryos, while preserving spatial organization at high resolution.

Technically, the initial setup went relatively smoothly. Ahilya Sawh (then in Susan Mango’s lab at the Biozentrum, now leading her own group at the University of Toronto, Canada) had previously established a FISH-based system for chromosome tracing in C. elegans [2, 3]. With support from the Biozentrum Imaging Core Facility, we adapted this system onto a Nikon microscope, making it more accessible for biological applications. Having this foundation in place was reassuring—it meant that the challenge ahead was not starting from zero, but rather pushing something promising to its limits.

Making it work in a whole embryo

Early on, we realized that if we wanted to understand gene expression in a whole embryo, measuring just a handful of genes would not be enough. We needed to look at hundreds of genes at the same time, but without an impractical number of imaging rounds. This led us to explore MERFISH, which uses a combinatorial barcode system to identify genes across multiple rounds of imaging and allows many genes to be read out efficiently [4, 5]. In practice, it felt like a good balance between scale and feasibility for what we wanted to do.

While the foundation was in place, adapting MERFISH to whole embryos still required several key innovations. An important part of this effort was our close collaboration with Bogdan Bintu at UCSD in the USA. During his time in Xiaowei Zhuang’s lab at Harvard University, Bogdan developed high-throughput imaging approaches that combine MERFISH with 3D chromatin organization [6], contributing deep experience in both experimental and computational aspects. He had already begun implementing important technical improvements and generously shared his strategies with us, as well as providing essential support for the instrumentation and computational pipeline.

In the end, making this work required combining several key improvements, each addressing a different limitation of the system:

• Stability during long-term imaging

Whole-embryo MERFISH imaging can take weeks, as the sample must be imaged plane by plane, region by region, and across multiple hybridization rounds. Early on, this long-term imaging became one of the most frustrating bottlenecks. Using conventional approaches, in which mRNAs are anchored to a gel and repeatedly probed, we saw signals gradually fading over time. Even under carefully controlled RNase-free conditions, degradation was unavoidable.

The solution came from rethinking the problem: instead of anchoring RNA, we anchored the primary DNA probes using acrydite modifications. This seemingly simple shift made a huge difference. The signal remained stable even after a month of continuous imaging, and we could use harsh stripping conditions (80–100% formamide) without worrying about losing the probes. It was one of those moments where a technical fix suddenly makes everything feel possible.

• Seeing deeper without losing signal

Imaging deep tissues introduced another challenge. Spinning disk confocal microscopy is essential for thick samples, but it comes with reduced signal compared to widefield imaging. We initially explored computational approaches to recover signal, but in practice, signal and noise often looked too similar to confidently separate.

Instead, we turned to a physical solution: branching amplification. By boosting the signal at the molecular level, we were able to image more reliably, reduce exposure time, and make the data much more interpretable, especially in deeper regions of the embryo.

• Designing for flexibility

Another challenge was making the method usable in practice. Traditional MERFISH requires encoding the combinatorial barcode directly into the primary probes, effectively locking the experiment into a fixed readout strategy.

We wanted something more flexible. By introducing a gene-specific linker system, we decoupled probe design from readout. This means that researchers can design large probe libraries first, and decide later how to read them out—sequentially for a few genes, or combinatorially for many. This flexibility turned out to be important not just technically, but psychologically: it lowers the barrier to trying the method in the first place.

When the data started to speak

Once we had the data, the analysis moved quickly. I remember feeling a mix of excitement and disbelief: after spending three years building the system, suddenly everything was there at once. The work was tremendously accelerated by the incredible team behind it: Jakob El Kholtei and I co-developed the weMERFISH method and the data processing pipeline. KJ Jenie, an exceptionally talented undergraduate at the time in Bogdan’s lab, built the MERFISHEYES website (https://schier.merfisheyes.com), making the dataset accessible and explorable. A key component of the project came from my postdoctoral colleague Jialin Liu, who had generated a comprehensive scMultiomics dataset of zebrafish development to study the regulatory logic of cell type specification [7], and very generously made it available to us. Integrating his scMultiomics data with the weMERFISH data allowed us to comprehensively map gene expression and chromatin accessibility in space, creating a multiomic atlas. Mariona Colomer-Rosell performed the analysis of these multiomic data and helped uncover principles of tissue-specific gene regulation at the whole-embryo level. Bringing together these different pieces was essential for turning the dataset into something we could truly interpret.

One of the most fascinating aspects for me was the concept of “time” in development. In single-cell data, we often reconstruct “pseudotime” trajectories, but seeing these trajectories mapped into real space was incredibly satisfying. Along the zebrafish tail, for example, we could directly observe the progression from progenitors to differentiated cells as a spatial gradient. It was one of the first moments where the abstract and the physical truly aligned.

We also applied a spatial version of RNA velocity [8, 9], using nuclear versus cytoplasmic transcripts as a proxy for transcriptional dynamics. What surprised us was that, especially in early development, the inferred transcriptional dynamics mirrored physical cell movements during morphogenesis. At first glance, this feels intuitive. But the underlying mechanisms are very different: transcriptional regulation and cell movement are controlled by distinct processes. The fact that they align so closely suggests a deeper coupling between gene expression dynamics and morphogenesis. This was one of those observations that stayed with me, because it hints at something fundamental that we don’t yet fully understand.

Another memorable part of the journey was the path from preprint to publication. When we first posted the work on bioRxiv and launched the MERFISHEYES website, the response was immediate and very encouraging. People started exploring the data, reaching out with questions, and even visiting the lab to learn how to set up the method. Seeing the dataset being used so quickly made us realize that it could become a resource for the community much earlier than we had expected.

At the same time, the peer review process pushed the work in important ways. The reviewers appreciated the technology and the dataset, but also challenged us to go further, especially to better connect the method to biological questions and to take fuller advantage of the multimodal data. Addressing these comments led to substantial additions and improvements throughout the paper. We expanded the analysis of subcellular transcript localization, strengthened the RNA velocity framework, benchmarked data integration methods more rigorously, and added new analyses such as cell–cell communication.

Perhaps most importantly, the revision motivated us to develop MERFISH-FATE in collaboration with Guoqiang Yu’s group (Tsinghua University, China), integrating spatial transcriptomics with live imaging to directly link gene expression changes to morphogenetic movements. Specifically, we mapped corresponding regions between a weMERFISH embryo and a live-imaged embryo at early gastrulation, where cells had been tracked throughout development. We then followed these trajectories forward and mapped the descendant cells back to their corresponding regions at mid-gastrulation, effectively connecting gene expression patterns across time.

This became a great extension of the story and shifted the paper from a largely static atlas into a more dynamic view of development. We then spent months simply looking at how patterns evolve—scrolling through images, comparing stages, trying to build intuition. Across many genes, we saw surprisingly complex dynamics. One example is tbxta, which is expressed at the embryonic margin at both early and mid-gastrulation. It would be natural to assume this reflects simple inheritance. But when we incorporated cell dynamics, we found that some cells activate tbxta while others turn it off. What looked like a static pattern was actually the result of dynamic and opposing processes. Moments like this made me appreciate how much information is lost when we only look at snapshots, and how powerful it is to connect gene expression with cell behavior. This is a direction we are now continuing to explore in more depth in a recent preprint describing fate mapping in zebrafish embryogenesis and beyond [10].

Looking ahead: from description to understanding

weMERFISH provides a way to map gene expression and cellular states across intact, developing tissues in 3D. For me, a particularly exciting direction is to extend this approach to larger and more complex systems: whole organs, organoids, and beyond. Moving into these systems will allow us to study how spatial gene expression patterns scale with size and geometry, and how these patterns are adapted across evolution.

At the same time, a major direction in the field, in my view, is the integration of multiple modalities. In this work, we combined weMERFISH with chromatin accessibility and embryo morphogenesis, and this naturally raises broader questions: how does gene expression relate to 3D genome organization? To protein distribution? To lineage history and cell behavior? Each of these layers captures a different aspect of cellular identity, and I believe only by combining them can we begin to understand how cells make decisions in their native context.

This is also a direction I am eager to pursue in my own future work. These multimodal datasets are not just richer—they fundamentally change the type of questions we can ask. Instead of only describing patterns, we can begin to build models that explain how these patterns arise. We can start to ask causal questions: which molecular features predict a cell’s future behavior? Which spatial contexts bias fate decisions? And how conserved are these relationships across tissues and embryos?

Ultimately, I am particularly interested in understanding how variability between individual cells gives rise to robust and reproducible tissue structures. Development is remarkably reliable, despite underlying variability. Technologies like weMERFISH bring us closer to uncovering these principles, and to understanding how a single fertilized egg reliably gives rise to a complex organism.

Access the article:

Wan, Y., J. El Kholtei, I. Jenie, M. Colomer-Rosell, J. Liu, Q. Zhang, J. Navajas Acedo, L. Y. Du, M. Codina-Tobias, M. Wang, W. Zheng, E. Lin, T. H. Chuang, O. Mayseless, A. Sawh, S. E. Mango, G. Yu, B. Bintu, and A. F. Schier. 2026. “Whole-embryo spatial transcriptomics at subcellular resolution from gastrulation to organogenesis.” Science 391 (6790): eadt3439. https://doi.org/10.1126/science.adt3439.

References

1. Farrell, J.A., et al., Single-cell reconstruction of developmental trajectories during zebrafish embryogenesis. Science, 2018. 360(6392).
2. Sawh, A.N., et al., Lamina-Dependent Stretching and Unconventional Chromosome Compartments in Early C. elegans Embryos. Mol Cell, 2020. 78(1): p. 96–111 e6.
3. Sawh, A.N. and S.E. Mango, Multiplexed Sequential DNA FISH in Caenorhabditis elegans Embryos. STAR Protoc, 2020. 1(3): p. 100107.
4. Chen, K.H., et al., RNA imaging. Spatially resolved, highly multiplexed RNA profiling in single cells. Science, 2015. 348(6233): p. aaa6090.
5. Moffitt, J.R. and X. Zhuang, RNA Imaging with Multiplexed Error-Robust Fluorescence In Situ Hybridization (MERFISH). Methods Enzymol, 2016. 572: p. 1–49.
6. Su, J.H., et al., Genome-Scale Imaging of the 3D Organization and Transcriptional Activity of Chromatin. Cell, 2020. 182(6): p. 1641–1659 e26.
7. Liu, J., et al., Decoding the regulatory logic of specification and differentiation during vertebrate embryogenesis. bioRxiv, 2024.
8. La Manno, G., et al., RNA velocity of single cells. Nature, 2018. 560(7719): p. 494–498.
9. Bergen, V., et al., Generalizing RNA velocity to transient cell states through dynamical modeling. Nat Biotechnol, 2020. 38(12): p. 1408–1414.
10. Wang, M., et al., High-Fidelity Long-term Whole-embryo Lineage and Fate Reconstruction by Iterative Tracking with Error Correction. bioRxiv, 2026: p. 2026.03. 12.711203.

The post From fate to form: the journey to whole-embryo spatial transcriptomics appeared first on the Node.

The preprints this month are hosted on bioRxiv – use these links to get to the section you want.

Developmental biology

• Patterning & signalling
• Morphogenesis & mechanics
• Genes & genomes
• Stem cells, regeneration & disease modelling
• Plant development
• Evo-devo

Cell Biology

Modelling

Reviews

Tools & Resources

Research practice & education

Developmental biology

| Patterning & signalling

Lineage domains and cytoskeletal cables organize a cellular square grid in a crustacean
Beatrice L. Steinert, Leo Blondel, Chandrashekar Kuyyamudi, Evangelia Stamataki, Anastasios Pavlopoulos, Cassandra G. Extavour

An optogenetic toolkit for robust activation of FGF, BMP, & Nodal signaling in zebrafish
Leanne E. Iannucci, Velanganni Selvaraj Maria Thomas, William K. Anderson, Micaela R. Murphy, Caitlin E.T. Donahue, Catherine E. Campbell, Matthew T. Monaghan, Allison J. Saul, Katherine W. Rogers

Directed conversion of porcine extended pluripotent stem cells into trophoblast-like stem cells through modulation of conserved TGF-β and ERK signaling pathways
Chi-Hun Park, Young-Hee Jeoung, JiTao Wang, Bhanu P. Telugu

Synthetic germ granules reveal a direct role of Vasa/DDX4 in RNA localization and translational activation
Ruoyu Chen, Henoc Zinga, Jay S. Goodman, Ruth Lehmann

Determinants of the Transition Zone Width of Morphogen Readouts
Jan A. Adelmann, Roman Vetter, José M. Dias, Johan Ericson, Dagmar Iber

Mechanistic tradeoffs between local and long-range signaling activity in natural and synthetic morphogens
Gavin Schlissel, Anders S. Hansen, Pulin Li

Inflammatory IL-1 signaling remodels epidermal stem cell compartments by suppressing Wnt activity
Hung Manh Phung, Ikuto Nishikawa, Nguyen Thi Kim Nguyen, Aiya K. Yesbolatova, Ahmed M. Hegazy, Tomson Kosasih, Jun Aoi, Satoshi Fukushima, Sho Hiroyasu, Hitoshi Takizawa, Aiko Sada

Cajal-Retzius fate specification is disrupted by constitutive activation of β-Catenin in hem progenitors
Amrita Singh, Arpan Parichha, Debarpita Datta, Mallika Chatterjee, Shubha Tole

Kinetic Control of Out-Of-Equilibrium Dynamics in the RhoA Signaling Cascade Shapes Actomyosin Contractility
Serena Prigent Garcia, Étienne Pinard, Camille N. Plancke, Jing Li, Shashi Kumar Suman, Loan Bourdon, Christelle Gally, Taeyoon Kim, François B. Robin

Dorsal/NF-κB exhibits a dorsal-to-ventral mobility gradient in the Drosophila embryo
Hadel Al Asafen, Natalie M. Clark, Etika Goyal, Sadia Siddika Dima, Hung-Yuan Chen, Thomas Jacobsen, Rosangela Sozzani, Gregory T. Reeves

Position Dependent Feedback Drives Scaling and Robustness of Morphogen Gradients
Lewis Scott Mosby, Zena Hadjivasiliou

TGF-β signaling regulates epithelial permeability in Drosophila ovaries by modulating adhesion independent of actomyosin contractility
Harshath Amal, Thea Jacobs, Max Lohrberg, Stefan Luschnig

Reconstructing signaling histories of single cells via perturbation screens and transfer learning
Nicholas T. Hutchins, Miram Meziane, Claire Lu, Maisam Mitalipova, David Fischer, Pulin Li

Direct cell-to-cell transport of Hedgehog morphogen is aided by the diffusible carrier Shifted/DmWif1
Carlos Jiménez-Jiménez, Gustavo Aguilar, Clara Fernández-Pardo, Markus Affolter, Isabel Guerrero

Arginine Kinase 1 regulates energy homeostasis in Drosophila muscle development
Maria Paula Zappia, Anton Westacott, Hannah Cooke, Rhianna Geary, Libby Travers, Lucia de Castro, Oliver Carty, Maxim V Frolov

Weckle is a molecular switch that diverts Toll signalling from innate immunity towards growth by engaging Yki
Maria Dolores Perez-Sanchez, Guiyi Li, Martin Moncrieffe, Francisca Rojo-Cortés, Karina Malinovska, Emily Sample, Myles Maddick, Marta Moreira, Elizabeth Connolly, Anna Parsons, Roberto Feuda, Nick J. Gay, Alicia Hidalgo

Basement membrane mechanics drives patterned response to developmental signalling
Ana Raffaelli, Tom P.J. Wyatt, Claire S. Simon, Léa M.D. Wenger, Kathy K. Niakan, Ewa K. Paluch, Kevin J. Chalut

A single-cell transcriptomic map of the Xenopus mesonephros reveals conserved nephron patterning across vertebrate kidney forms
Mark E. Corkins, Adrian Romero, MaryAnne A. Achieng, Nils O. Lindström, Rachel K. Miller

Sharp cell-type boundaries emerge from temporal coordination between morphogen signals
Ruiqi Li, Yiqun Jiang, Sarah Platt, Tianchi Xin, Ryan Driskell, Kevin A. Peterson, Sarah Van, Hainan Lam, Shagun Lukkad, Eva-LaRue Barber, Chae Ho Lim, M. Mark Taketo, Yuval Kluger, Peggy Myung

Synthetic reconstitution of planar polarity initiation reveals collective migration as a symmetry-breaking cue
Leah A Wallach, Connor D Thomas, Pulin Li

Epithelial-Mesenchymal Wnt Crosstalk Directs Planar Cell Polarity in the Developing Cochlea
Ippei Kishimoto, Abel P. David, Kevin P. Rose, Balasubramanian Narasimhan, Bradley Efron, Sara E. Billings, Erin L. Su, Wuxing Dong, Taha A. Jan, Ronna Hertzano, Alan G. Cheng

Notch-driven fate asymmetry dictates hair cell behavior via a fate-specific kinase
Emily Atlas, Caleb C. Reagor, Brian Frost, Sapna Krishnakumar, A. J. Hudspeth, Adrian Jacobo

A synNotch-based morphogen detection system reveals sFRP2 enhances Wnt3a signaling
Kosuke Mizuno, Satoshi Toda

| Morphogenesis & mechanics

Tenascin N contributes to spinal motor nerve morphogenesis during development
Charles G. Marcucci, Marieke Jones, Coleman Blanton, Sarah Kucenas

A stress-responsive morphogenetic program of the uterine epithelium safeguards the establishment of early pregnancy
Chihiro Ishizawa, Shizu Aikawa, Yamato Fukui, Xueting He, Ryoko Shimizu-Hirota, Daiki Hiratsuka, Mitsunori Matsuo, Takehiro Hiraoka, Yasushi Hirota

Cell-intrinsic compliance mechanism enables release of tensile stress to prevent tissue rupture
Chun Wai Kwan, Shunta Sakaguchi, Michiko Takeda, Takefumi Kondo, Yu-Chiun Wang

The mechanosensitive protein Zyxin influences Hippo signalling and tissue growth via adherens junctions and basal spot junctions in Drosophila
Harmanjeet Singh, Elliot Brooks, Kyoko Jinnai, Shu Kondo, Samuel A. Manning, Benjamin Kroeger, Kieran F. Harvey

Inverted Assembly of the Lens Within Ocular Organoids Reveals Alternate Paths to Ocular Morphogenesis
Elin Stahl, Miguel Angel Delgado-Toscano, Ishwariya Saravanan, Anastasija Paneva, Joachim Wittbrodt, Lucie Zilova

Long-Range Coupling of Posterior Cell Addition and Anterior Vacuolation Provides Robustness in Notochord Elongation
Carlos Camacho-Macorra, Alberto Ceccarelli, Dillan Saunders, Guillermo Serrano Nájera, Osvaldo Chara, Benjamin Steventon

Pre-cuticle DPY-6 acts as a blueprint for aECM periodic organization in C. elegans
Sophie Mazzoli, Thomas Sonntag, Emma Cadena, Claire Valotteau, Susanna K. Birnbaum, Meera V. Sundaram, Nathalie Pujol

Unified Transcriptome and Mechanics Map of the Intact Mammalian Preimplantation Embryo In Situ
Ehsan Habibi, Anubhav Sinha, Haiqian Yang, Payman Yadollahpour, Yiwei Li, Lani Lee, David A. Wollensak, Zachary D. Chiang, Denny Sakkas, Edward S. Boyden, Ming Guo, Aviv Regev, Fei Chen

A single-cell transcriptomic atlas of inner ear morphogenesis in zebrafish
Akankshi Munjal, Kalki Kukreja, Samara Williams, Toru Kawanishi, Natasha M. O’Brown, Kana Ishimatsu, Allon Klein, Sean G. Tsung-Megason, Ian A. Swinburne

Kindlin-2-Moesin interaction orchestrates sprouting angiogenesis via modulating endothelial membrane mechanics and VEGF signaling
Lu Wang, Yuxin Fu, Zeyang Yu, Yi Lei, Tianjing Yang, Jiayu Liu, Nina Ma, Yuming Liu, Kunfu Ouyang, Kai Zhang, Junhao Hu, Xi Fang, Ying Shen, Jing Zhou, Xiaohong Wang

Tissue-scale mechanics controls differentiation strategy and dynamics of epithelial multilayering
Clémentine Villeneuve, Somiealo Azote Epse Hassikpezi, Marga Albu, Matthias Rübsam, Leah C. Biggs, Sabrina Vinzens, Kai Kruse, Anubhav Prakash, Peter Zentis, Elizabeth Lawson-Keister, Gautier Follain, Johanna Ivaska, Carien M. Niessen, M. Lisa Manning, Sara A. Wickström

Mechanical memory of confinement pressure governs expansion size in epithelial monolayers
Linn Engström, Simon K Schnyder, Johannes K Ahnlide, Valeriia Grudtsyna, Martijn Gloerich, Pontus Nordenfelt, Amin Doostmohammadi, Vinay Swaminathan

| Genes & genomes

Light-entrained chromatin priming poises rapid metamorphosis in a marine sponge
Huifang Yuan, Oceane Blard, Zac Pujic, Bernard M. Degnan, Sandie M. Degnan

Single-cell multiomics identifies key nodes and cis-regulatory elements of the networks specifying the eye domains in zebrafish
Javier Macho Rendón, Rocío Polvillo, Álvaro Gónzalez-Cid, Jorge Corbacho, Silvia Naranjo, Sofia Manzo, Ana Sousa-Ortega, Ana Fernández-Miñán, Juan Tena, Juan Ramón Martínez-Morales

Reciprocal zebrafish-medaka hybrids reveal maternal control of zygotic genome activation timing
Krista R. Briedis-Gert, Gunnar Schulze, Maria Novatchkova, Karin Panser, Luis Enrique Cabrera Quio, Anja Koller, Yixuan Guo, Bradley R. Cairns, Eivind Valen, Andrea Pauli

A Multi-tissue Transcriptomic-Metabolomic Map Linking Maternal High-Fiber Diet to Reduced Offspring Type 2 Diabetes
Tetsuto Katsura, Oluwagbotemi Omojola, Antwi-Boasiasko Oteng, Peng Jiang, Katherine A. Overmyer, Josh Coon, Amadou Gaye, Huishi Toh

A transcriptional code controlling fluid shear stress-induced gene expression
Lucija Fleisinger, Susann Bruche, Hyewon Lim, Anna Rataj, Helena Rodriguez-Caro, Amaury Genovese, Vinesh Vinayachandran, Svanhild Nornes, Dorota Szumska, Dhruv S Gupta, Indrika Ratnayaka, Kira Chouliaras, Marek Giers, Simon J Conway, Alice Neal, Sophie Payne, Martin A Schwartz, Mukesh K Jain, Brian G Coon, Sarah De Val

Single-cell multiomic approaches define a gradual, spatially-regulated epigenetic and transcriptional transition from embryonic to adult neural stem cells
Beatrix S. Wang, Konstantina Karamboulas, Nareh Tahmasian, Daniel J. Dennis, David R. Kaplan, Freda D. Miller

Male fertility is independent of Enh13 control of Sox9 testicular expression
Maor Lubman, Meshi Ridnik, Isabelle Stévant, Yael Kimchi Djanshvili, Elisheva Abberbock, Shelly Ziv Lhermann, Nitzan Gonen

A dual-phase enhancer couples progenitor maintenance and pancreatic lineage stability
Marta Duque, João Amorim, Joana Teixeira, Beatriz Custódio, Mafalda Galhardo, Francisco Camões Magalhães, Joana Marques, Ana Paula Pêgo, José Bessa

Enhancer-mediated metabolic pre-patterning defines trabecular cardiomyocyte identity prior to morphogenesis
Costantino Parisi, Shikha Vashisht, Mohammad Salar Ghasemi Nasab, Kandhadayar Gopalan Srinivasan, Katarzyna Misztal, Marcin Zagorski, Cecilia Winata

ERH elicits cell lineage restriction in mammalian preimplantation development and differentiation from pluripotency via H3K9me3-heterochromatin
Andrew Katznelson, Blake Hernandez, Kylea Tapia, Holly Fahning, Adam Burton, Jingchao Zhang, Maria-Elena Torres-Padilla, Nicolas Plachta, Kenneth S. Zaret, Ryan L. McCarthy

A conserved C. elegans zinc finger-homeodomain protein, ZFH-2, continuously required for structural integrity and function of alimentary tract and gonad
Antoine Sussfeld, Berta Vidal, Surojit Sural, Daniel M. Merritt, G. Robert Aguilar, Yasmin Ramadan, Oliver Hobert

The Nkx2.3–Nr5a1 gene cascade plays a crucial role in spleen-specific vascular architecture and marginal zone formation
Kanako Miyabayashi, Koji Ono, Tetsuya Sato, Ayano Yahagi, Masanori Iseki, Katsuhiko Ishihara, Takami Mori, Miki Inoue, Ryuki Shimada, Kei-ichiro Ishiguro, Tomohiro Ishii, Jongsung Noh, Man Ho Choi, Takashi Baba, Yasuyuki Ohkawa, Emi Kiyokage, Kazunori Toida, Yuichi Shima

A Phospho-Switch for Cell Fate Control
Jin Ming, Xianzhuang Liu, Zexiao Jia, Wei Shi, Jiajun Li, Shikun Wang, Yulin Chen, Shixian Lin, Yu Liang, Peng Guo, Hanqing Zhao, Yuxiang Yao, Ruona Shi, Xiaofei Zhang, Yuanyue Shan, Yu Fu, Bo Wang, Chengchen Zhao, Duanqing Pei

Mitotic bookmarking by Prox1 preserves mammalian neuronal lineage identity memory via promoting timely H3K27me3 restoration
Chouin Wong, Jie Liu, Haoran Yang, Haotian Li, Xiaoqi Luo, Tingyi Li, Zili Chen, Jingyi Chu, Yuying Shen, Shuai Long, Yong Zhang, Yan Song

A Mediator-dependent hypertranscriptional program governs neural stem cell fate decisions in vivo
Tiago Baptista, Daniela Lopes, Ana Rita Rebelo, Catarina CF Homem

Single-Cell Atlas of Transcription and Chromatin States Reveals Regulatory Programs in the Human Brain
Yang Xie, Lei Chang, Guojie Zhong, Jonathan A. Rink, Tatiana Báez-Becerra, Ethan Armand, Wubin Ding, Kai Li, Eric Bonne, Audrey Lie, Hannah S Indralingam, Keyi Dong, Timothy Loe, Bohan Huang, Zhaoning Wang, Ariana S. Barcoma, Jackson K. Willier, Kyle W. Knutson, Jiayi Liu, Silvia Cho, Stella Cao, Kaitlyn G. Russo, Carissa K. Young, Jessica Arzavala, Yareli Sanchez, Aleksandra Bikkina, Natalie Schenker-Ahmed, Colin Kern, Zoey Zhao, Amit Klein, Jesus Flores, Chu-Yi Tai, Jacqueline Olness, Alexander Monell, Siavash Moghadami, Cesar Barragan, Chumo Chen, William Owens, Carolyn O’Connor, Michelle Liem, Mikayla V. Marrin, Cynthia Rose, Shane N. Alt, Nora Emerson, Julia Osteen, Jacinta Lucero, Daofeng Li, Rebecca D. Hodge, Ting Wang, C. Dirk Keene, Xiangming Xu, Quan Zhu, Joseph R. Ecker, M. Margarita Behrens, Bing Ren

DNA supercoiling links transcription and chromatin architecture during human stem cell differentiation
Consuelo Perez, Pierre Murat, Andrew Zeller, Kim C. Liu, Alastair Crisp, Julian E. Sale

Matched single-cell chromatin, transcriptome, and surface marker profiling captures in vivo epigenomic reprogramming during basal-to-luminal transition in the mammary gland
Anna Schwager, Eve Moutaux, Adeline Durand, Alexandra Van Keymeulen, Amélie Viaene, Mélanie Miranda, Louisa Hadj Abed, Simon Besson-Girard, Marion Lambault, Délia Dupré, Grégoire Jouault, Mélissa Saichi, Juliette Bertorello, Simon Dumas, Mathias Schwartz, Marthe Laisné, Justine Marsolier, Manuel Guthmann, Lorraine Bonneville, Urvashi Chitnavis, Déborah Bourc’his, Elisabetta Marangoni, Nicolas Servant, Cédric Blanpain, Leïla Perié, Céline Vallot

| Stem cells, regeneration & disease modelling

Neonatal diethylstilbestrol exposure disrupts uterine epithelial apical-basal polarity and partial EMT state
Rachel E. Bainbridge, Wendy N. Jefferson, Tianyuan Wang, Sara A. Grimm, Carmen J. Williams

PIK3CA-related overgrowth spectrum (PROS) zebrafish models reveal pan-lineage developmental dysregulation
Hannah Brunsdon, Nuoya Wang, Micha Sam Brickman Raredon, Ralitsa R Madsen, Robert K Semple, E Elizabeth Patton

Maternal-fetal immune conflict contributes to male-specific impairments in a mouse model of neurodevelopmental disorders
Irene Sanchez-Martin, Bharti Kukreja, Paige Henderson, Qianyu Lin, Daniel DiMartino, Valerie Bagan, Justin Park, Brian T. Kalish, Lucas Cheadle

PRDM16 is necessary for sensory neuronal development in the Trigeminal Ganglion
Fahmida Raha, Qiman Gao, Lomeli C. Shull, Kristin B. Artinger

Leucettinib-21 decreases dosage effects of DYRK1A in human trisomy 21 iPSC-derived neural cells
Nicole R. West, Mattias F. Lindberg, Julien Dairou, Shawn MacGregor, Sahith Puthireddy, Laurent Meijer, Anita Bhattacharyya

Mutant FGFR3 restricts bone yet expands cortex via ERK-mediated self-repression
Zhuangzhi Zhang, Zhejun Xu, Tongye Fu, Wenhui Zheng, Zizhuo Sha, Chuannan Yang, Feihong Yang, Jialin Li, Jing Ding, Zhengang Yang

Hypoxia differentially affects coronary vessel formation during heart development
Sophie Payne, Susann Bruche, Dorota Szumska, Alice Neal, Mark D Preston, Sarah De Val

Pancreatic Duct Cells as a Potential Source for Human Islet Neogenesis: Insights from Imaging Mass Cytometry
Rui Liang, Tengli Liu, Lanqiu Zhang, Wenmiao Ma, Huixia Ren, Shusen Wang

Rp-vasa: a bona fide Primordial Germ Cell marker that drives embryonic expression in the Chagas disease vector Rhodnius prolixus
G. Martins, M. Berni, T. Guedes-Silva, D. Bressan, J. Vieira, M. Cardoso, A. Pane, V. Gantz, E. Bier, H.M Araujo

Two axolotl-adapted cell-ablation platforms reveal macrophage-dependent processes essential for spinal-cord and skeletal regeneration
Gabriela Johnson, Andrew Hart, Markus Sujansky, Joel H. Graber, James W. Godwin

The regulatory landscape of optic fissure closure in the vertebrate eye
Brian Ho Ching Chan, Mariya Moosajee, Holly Hardy, James Prendergast, Joe Rainger

Evidence that injury can cause Drosophila gut differentiated, polyploid enterocytes to be recruited as stem cells via paligenosis
Dongkook Park, Robert M. Lawrence, Tyler Jackson, Hongjie Li, Jason C. Mills

The abnormal C-terminus in DVL1 impacts Robinow Syndrome phenotypes
Shruti S. Tophkhane, Gamze Akarsu, Sarah J. Gignac, Katherine Fu, Stephanie Xie, Esther M. Verheyen, Joy M. Richman

HSD17B7 is required for the function of sensory hair cells by regulating cholesterol synthesis
Yuqian Shen, Ziyang Wang, Xun Wang, Fuping Qian, Mingjun Zhong, Xin Wang, Jing Cheng, Dong Liu

Regeneration can take place across Drosophila compartments or segments with different Hox gene expression
Rafael Alejandro Juárez-Uribe, Paloma Martín, Laura Utiel, Blanca L. Arrabal, Marina Blanco, Roberto Yagüe-Serrano, Eduardo Cazalla, Ernesto Sánchez-Herrero

Intellectual disability risk gene RFX4 regulates cortical neurogenesis by restraining neuronal differentiation
Julianna J. Determan, Gareth Chapman, Sydney R. Crump, Faiza Batool, Sofia Malik, Taranjit S. Gujral, William Buchser, Caleb Valentine, Serena Elia, Monica Sentmanat, Xiaoxia Cui, Haley Jetter, Kristen L. Kroll

Brain morphological pattern is associated with the presence, severity, and transition of transdiagnostic psychiatric disorders in preadolescents
Nanyu Kuang, Christopher J Hammond, Betty Jo Salmeron, Xiang Xiao, Danni Wang, Laura Murray, Hong Gu, Tianye Zhai, Hui Zheng, Justine Hill, Maria Scavinicky, Hanbing Lu, Amy Janes, Thomas J Ross, Yihong Yang

4D Single-Cell Spatial Transcriptomics Reveals Dynamic Morphogenetic Gradients and Regenerative Domains in Planarians
Kai Han, Yue Chen, Yao Li, Lidong Guo, Yuxiaofei Wang, Xiawei Liu, Yaru Lin, Zhi Huang, Qun Liu, Wenjie Guo, Rui Zhang, Wandong Zhao, Langchao Liang, Xiaoyu Wei, Li Zhou, Xuebin Mao, Jiaqi Wang, Weijian Wu, Hongwei Pan, Tao Yang, He Zhang, Xiaoshan Su, Shanshan Liu, Wenwei Zhang, Longqi Liu, Søren Tvorup Christensen, Jifeng Fei, Xin Liu, Guangyi Fan, Hanbo Li, Ying Gu, Jian Wang, Huanming Yang, Gang Pei, Xun Xu, An Zeng, Mengyang Xu

Drosophila ryanodine receptor gene triggers functional and developmental muscle properties and could be used to assess the impact of human RYR1 mutations
Monika Zmojdzian, Teresa Jagla, Florian Cherik, Magda Dubinska-Magiera, Marta Migocka-Patrzalek, Malgorzata Daczewska, John Rendu, Krzysztof Jagla, Catherine Sarret

Asymmetric Histone Inheritance Regulates Olfactory Stem Cell Fates During Regeneration
Binbin Ma, Guanghui Yang, Jonathan Yao, Charles Wu, Jean Pinckney Vega, Gabriel Manske, Saher Sue Hammoud, Satrajit Sinha, Abhyudai Singh, Haiqing Zhao, Xin Chen

A knock-in Six2Cre line reveals transient interstitial potential in nephron progenitors
Azadeh Haghighitalab, Fariba Nosrati, Zeinab Dehghani-Ghobadi, Mohammed Sayed, Christopher Ahn, Yueh-Chiang Hu, Eunah Chung, Hee-Woong Lim, Joo-Seop Park

Lack of specificity of progenitor responses to injury in regeneration
Cecilia E. Pellegrini, Peter W. Reddien

Unique mineralization pattern revealed in TBCK syndrome mouse model
Kaitlin A. Katsura, Yuchen Jiang, Marius Didziokas, Nir Z. Badt, Sonia Dougherty, Kyle H. Vining, Elizabeth J. Bhoj

Pre-clinical models of idiopathic scoliosis implicate sex-specific roles for complement activity in modulating spinal curve severity
Vida Erfani, Brian Ciruna

Adipocyte-Derived Amino Acid Storage Proteins are Required for Germline Stem Cell Maintenance in Adult Drosophila Females
Anna B. Zike, Mekenzi O. Hazen, Madison G. Abel, Eleanor B. Goldstone, Robert C. Eisman, Lesley N. Weaver

Cell-autonomous Wnt activity promotes transient re-programming and cell cycle re-entry of coronary artery endothelial cells
Bhavnesh Bishnoi, Alfia Nirguni Saini, Vinay Rao, Omkar Golatkar, Ravindra Kailasrao Zirmire, Shruthi Viswanath, Perundurai Subramaniam Dhandapany, Soumyashree Das

Niche-dependent modular regulation of the stem cell transcriptome separates cell identity and potential
Amelie Raz, Hafidh Hassan, Yukiko Yamashita

TNAP and PHOSPHO1 function synergistically to afford critical control over the mineralisation of the postnatal murine skeleton
Lucie E Bourne, Aikta Sharma, Scott Dillon, Jacob Keen, Soher N Jayash, Natalie Crump, Lucinda AE Evans, Maya Karmali, Worachet Promruk, Claire E Clarkin, Sonoko Narisawa, Louise Stephen, Brian L Foster, José Luis Millán, Colin Farquharson, Katherine A Staines

Spinal cord regeneration deploys adult molecular programs that do not recapitulate embryonic development
Yuxiao Xu, Amulya Saini, Wenda Zhang, Lili Zhou, Mayssa H. Mokalled

Tissue composition shapes differential skeletal integration strategies during axolotl limb regeneration
Rita Aires, Sean D. Keeley, Kerstin Brandt, Mário Carreira, Doğa Berşan Güneş, Yagiz Savci, Ulrike Anne Friedrich, Andreas Dahl, Can Aztekin, Tatiana Sandoval-Guzmán

Endocardial TIE1 synergizes with TIE2 to regulate the atrial internal muscular network assembly
Kai Ding, Beibei Xu, Xinhao Yu, Xiwen Jia, Taotao Li, Xin Shen, Junda Li, Xudong Cao, Yahui Liu, Zhen Zhang, Yulong He

Hypoxia-activated scleraxis a mediates epicardial progenitor differentiation into a unique cardiac perivascular cell type
Björn Perder, Yu Xia, Jun Yao, Miaoyan Qiu, Alvin Gea Chen Yao, Muhammad Naeem, Paul Zumbo, Ignace Van der Wee, Avi Yakubov, Kazu Kikuchi, Doron Betel, Todd Evans, Michael R. Harrison, Jingli Cao

| Plant development

A Functional Basis for the Developmental Sequence of the Macrostructure of the Venus Flower Basket (Euplectella aspergillum)
Y. Mistry, S. Morankar, D. Kingsbury, N. Chawla, C. A. Penick, D. Bhate

Naturally occurring variation in gene-associated transposable elements impacts gene expression and phenotypic diversity in woodland strawberry
Santiago Priego-Cubero, Rocio Tolley, Julia Llinares-Gómez, Camila Zlauvinen, Tuomas Toivainen, Timo Hytönen, Carmen Martín-Pizarro, Ileana Tossolini, Pablo A. Manavella

Toxic metals increase root hair density by reducing epidermal cell length
Julia Zheku, Thea Do, M. Arif Ashraf

Extracellular calcium modulates pollen tube growth and guidance in Arabidopsis thaliana
Kumi Matsuura-Tokita, Yoko Mizuta, Daisuke Kurihara, Tetsuya Higashiyama

WUSCHEL modulates jasmonate signaling to control the balance between growth and defense in the shoot apical meristem
Pengfei Fan, Panagiotis Boumpas, Christian Wenzl, Yanfei Ma, Gernot Poschet, Jiao Zhao, Thomas Greb, Jan U. Lohmann

Mechanical coordination of counter-gradient growth maintains organ curvature in the apical hook
Sara Raggi, Hemamshu Ratnakaram, Adrien Heymans, Loitongbam Lorinda Devi, Özer Erguvan, Siamsa M. Doyle, François Jobert, Asal Atakhani, Sijia Liu, Manuel Petit, Jürgen Kleine-Vehn, Krzysztof Wabnik, Stéphane Verger, Stéphanie Robert

A visualization framework for cell division activity and orientation in pre-anthesis ovaries of Prunus species
Ayame Shimbo, Soichiro Nishiyama, Tatsuya Katsuno, Akane Kusumi, Hisayo Yamane, Masahiro M. Kanaoka, Ryutaro Tao

Transformers Outperform ConvNets for Root Segmentation: A Systematic Comparison Across Nine Datasets
Abraham George Smith, Sotiris Lamprinidis, Anand Seethepalli, Larry M. York, Eusun Han, Patrick Möhl, Kyriaki Boulata, Kristian Thorup-Kristensen, Jens Petersen

Evolution of moss leaf-like organs through variations in deeply conserved developmental principles
Wenye Lin, Loann Collet, Laure Mancini, Mandar Deshpande, Brendan Lane, Benjamin P. Lapointe, Agnieszka Bagniewska-Zadworna, Anne-Lise Routier-Kierzkowska, Richard S. Smith, Yoan Coudert, Daniel Kierzkowski

Ca²⁺ oscillations promote microtubule-band turnover and support tip growth in Arabidopsis zygotes
Hikari Matsumoto, Zichen Kang, Tomonobu Nonoyama, Yusuke Kimata, Satoru Tsugawa, Minako Ueda

Effects of lovastatin on auxin transport and root development in Arabidopsis thaliana
Veronica Giourieva, Christos Tersenidis, Alkiviadis Athanasiadis, Stylianos Poulios, Anna Kouskouveli, Konstantinos Vlachonasios, Emmanuel Panteris, George Komis

| Eco-evo-devo

Ancient origin of the dorso-ventral patterning system of vertebrate paired fins
Rebecca E. Dale, Silke Berger, Sara Alaei, Adele Barugahare, Marcus C. Davis, Laura Perlaza-Jimenez, Frank J. Tulenko, Peter D. Currie

Embryonic and larval development of the Pacific saury Cololabis saira: Distinctive characteristics of a rapidly growing beloniform fish
Rie Kusakabe, Shinya Yamauchi, Shigehiro Kuraku

Age- and Light-Dependent Changes in the Zebrafish Olfactory Epithelium
George B. Chapman, Rania Abutarboush, Victoria Connaughton

Thrifty phenotypes in ants: Extending a human developmental hypothesis to a superorganism
Érik Plante, Ehab Abouheif, Jean-Philippe Lessard

Thermal Plasticity of Stage-specific Development Time and Adult Body Size under Temperature Shifts: A Case Study Using Drosophila melanogaster
Aradhya Chattopadhyay, Rishav Roy, Payel Biswas, Shampa M. Ghosh

The dynamic evolution of panarthropod germ cell specification mechanisms
Jonchee A. Kao, Emily L. Rivard, Rishabh R. Kapoor, Cassandra G. Extavour

Annelid eye evolution revealed by developmental, ultrastructural, and connectome analyses of cerebral eyes in Malacoceros fuliginosus
Suman Kumar, Anna Seybold, Oleg Tolstenkov, Sharat Chandra Tumu, Harald Hausen

Tracking morphological development in stony corals
Garrett J. Fundakowski, Viviana Brambilla, Kyle J. A. Zawada, Cher F Y Chow, Emily Croasdale, Amelia J. F. Errington, Luisa Fontoura, Wilhelm J Marais, Rachael M. Woods, Pim Edelaar, Kevin Lala, Joshua S. Madin, Maria Dornelas

Cell Biology

Whole-Cell Proteomics Identifies Novel Regulators of Ciliogenesis Beyond the Axoneme
Xiaolu Xu, Yanbao Yu, Tony Zheng, Fiona Clark, Jean Ross, Neha Sindhu, Andre L P Tavares, John B Wallingford, Shuo Wei, Jian Sun

Developmentally programmed nuclear pore complex replacement enables oocyte specification
Shruti Venkat, Tram Nguyen, Cecilia Blangini, Michelle Pollak, Karen Schindler, Maya Capelson, Prashanth Rangan

PKN is a sex- and species-specific fertilization factor in brown algae
Masakazu Hoshino, Meri Nehlsen, Rita A. Batista, Morgane Raphalen, Toshiyuki Wakimoto, Shinya Uwai, Kazuhiro Kogame, Vikram Alva, Susana Coelho

HDAC1/2-mediated repression of Wnt receptor expression orients asymmetric division polarity in C. elegans
Mar Ferrando-Marco, Beatriz Garcia del Valle, Mark Hintze, Lucy Narunsky, Shuxiao Lin, Junyue Huang, Shannon Edwards, Michalis Barkoulas

Forward Programming Identifies Inducers of Blood-Brain Barrier Properties in Human Pluripotent Stem Cell-Derived Endothelial Cells
Soniya Tamhankar, Yunfeng Ding, Fatemeh Yaghoobi Hashjin, Sarah M. Boutom, Richard Daneman, Sean P. Palecek, Eric V. Shusta

Restoration of Spermatogenesis is Dependent on Activation of a SPRY4-ERK Checkpoint Following Germline Stem Cell Damage
Ying Liu, Tansol Choi, Brad Pearson, Ryan Nachman, Whitney Woo, Na Xu, Ryan Schreiner, Romulo Hurtado, Marco Seandel, Shahin Rafii, Todd Evans

Microtubules sustain the fidelity of cellularization in a coenocytic relative of animals
Margarida Araújo, Marine Olivetta, Paolo Ronchi, Viola Oorschot, Arif Khan, Christian Tischer, Hiral Shah, Gautam Dey, Omaya Dudin

Mechanosensing and IL-13 Signaling Synergistically Modulate Intestinal Stem Cell Differentiation via STAT6 and YAP
Sarbari Saha, Thao Nguyen, Cornelis Mense, Marie Touzet-Robin, Karen Kresbach, Stephan A. Eisler, Ulrich S. Schwarz, Andrew G. Clark

Altered stem cell properties of human hematopoietic stem and progenitor cells based on bone region location
Christopher J. Wells, Christine Hall, Samantha M. Holmes, Isabelle J. Grenier-Pleau, John F. Rudan, Steve Mann, Sheela A. Abraham

Modelling

Segmented wavetrains and sites of reversal in the mouse seminiferous tubules
Kei Sugihara, Ayuki Sekisaka, Toshiyuki Ogawa, Takashi Miura

Early development of male germ cell clones shapes their reproductive success
Tatsuro Ikeda, Maurice Langhinrichs, Tamar Nizharadze, Chieko Koike, Yuzuru Kato, Katsushi Yamaguchi, Shuji Shigenobu, Kana Yoshido, Shinnosuke Suzuki, Toshinori Nakagawa, Ayumi Maruyama, Seiya Mizuno, Satoru Takahashi, Nils B. Becker, Hans-Reimer Rodewald, Thomas Höfer, Shosei Yoshida

A mathematical synthesis of genetics, development, and evolution
Mauricio González-Forero

Tools & Resources

Cellular diversity of the developing chick trigeminal ganglion at single-cell resolution
Arvind Arul Nambi Rajan, Erica J. Hutchins

A quantitative in vivo CRISPR-imaging platform identifies regulators of hyperplastic and hypertrophic adipose morphology in zebrafish
Rebecca Wafer, Panna Tandon, James E. N. Minchin

A robust human airway organoid platform enables scalable expansion and trajectory mapping of pulmonary neuroendocrine cells
Noah Candeli, Lisanne den Hartigh, Nicholas Hou, Andrés Marco, José Antonio Sánchez-Villicaña, Andrea García-González, Shashank Gandhi, Francesca Sgualdino, Alyssa J. Miller, Jason Spence, Susana Chuva de Sousa Lopes, José L. McFaline-Figueroa, Hans Clevers, Talya L. Dayton

Nuclear Histone 3 Post-Translational Modification Profiling in Whole Cells using Spectral Flow Cytometry
Carly S. Golden, Saylor Williams, Sandeep Sreerama, Sophia Blankevoort, H. Joseph Yost, Martin Tristani-Firouzi, Anna Belkina, Maria A. Serrano

Epitope-based labeling for improved live-imaging of endogenous proteins in C. elegans
Elise van der Salm, Mette H. Schroeder, Loes B. Steller, Stephanie I. Miller, Amelie Scheper, Gwen Nowee, Erik. E. Griffin, Suzan Ruijtenberg

A facile method for fluorescent visualization of newly synthesized fibrous collagen by capturing allysine aldehyde groups as cross-link precursors
Junpei Kuroda, Kazunori K. Fujii, Sugiko Futaki, Azumi Hirata, Yuki Taga, Takaki Koide

Electrical stimulation combined with p27Kip1 inactivation drives proliferative neurogenic reprogramming of Mueller glia in the adult mouse retina
Megan L. Stone, Joel Jovanovic, Edward M. Levine

Arrayed single-gene perturbations identify drivers of human anterior neural tube closure
Roya E. Huang, Giridhar M. Anand, Heitor C. Megale, Jason Chen, Chudi Abraham-Igwe, Sharad Ramanathan

Conserved cellular architecture and developmental mechanisms of the zebrafish meninges
Ashley L. Arancio, Kathryn Wilhem, Hung-Jhen Chen, Brandon M. Hernandez, Percy J. Raggi, D’Juan T. Farmer

A Single-Cell Temporal Atlas of Mouse Nasal Embryonic Development
Huan Chen, Yingxiu Chen, Mengjie Pan, Ziyu Feng, Baomei Cai, Yiyi Cheng, Sihao Chen, Jiehong Deng, Xia Yao, Chunhua Zhou, Yunjing Du, Wei He, Ruifang Zhang, Yudong Fu, Shujuan Liu, Lihui Lin, Shengyong Yu, Yuehong Yan, Duanqing Pei, Dajiang Qin, Jiekai Chen, Shangtao Cao

Cell Type Architecture and Positional Gene Gradients in an Adult Animal at Subcellular Resolution
Maoqin Sun, Yuxiaofei Wang, Kai Han, Lidong Guo, Yue Chen, Yao Li, Yaru Lin, Xiawei Liu, Zhi Huang, Qun Liu, Wenjie Guo, Rui Zhang, Wandong Zhao, Langchao Liang, Xiaoyu Wei, Li Zhou, Xuebin Mao, Jiaqi Wang, Weijian Wu, Hongwei Pan, Tao Yang, He Zhang, Xiaoshan Su, Shanshan Liu, Wenwei Zhang, Longqi Liu, Søren Tvorup Christensen, Jifeng Fei, Xin Liu, Ying Gu, Jian Wang, Huanming Yang, Gang Pei, Guangyi Fan, Xun Xu, Hanbo Li, Mengyang Xu, An Zeng

Husbandry and Maintenance of Carausius morosus Laboratory Populations
Macy Ingersoll, Petra Kovacikova, Yousuf Hashmi, Cassandra G. Extavour

A molecular and spatial resource defining tubulin isotype organization during corneal development
R Ramarapu, WR Stoehr, M Miesen, S. Border, SM Thomasy, CD Rogers

Efficient derivation and transcriptional characterization of mouse extra-embryonic endoderm stem cell lines generated by somatic cell nuclear transfer
Shuaipeng Li, Shu Wei, Guomeng Li, Mei Hu, Jiangwei Lin, Wandong Bao

Integrative Inference of Spatially Resolved Cell Lineage Trees using LineageMap
Xinhai Pan, Yiru Chen, Xiuwei Zhang

Observing concurrent subcellular dynamics in large living tissues
Charles S Wright, Sanjeev Uthishtran, Laura Z Kreplin, Hetvi R Gandhi, Abhishek Patil, Harrison M York, Samyukta Sita, Samuel A Manning, Elliot Brooks, Guizhi Sun, In-won Lee, Wing Hei Chan, Sara Hlavca, Samuel Crossman, Helen E Abud, Jan Kaslin, Avnika A Ruparelia, Peter D Currie, Kieran F Harvey, Jose M Polo, John Carroll, Senthil Arumugam

High resolution spatial transcriptomic and proteomic profiling of early primate gastrulation in utero
Nikola Sekulovski, Maliha Kabir, Anusha Rengarajan, Amber E. Carleton, Jenna K. Schmidt, Chien-Wei Lin, Kenichiro Taniguchi

Single-cell-scale spatial transcriptome reveals early regional priming of the developing mouse ovary
Anthony S. Martinez, Tyler J. Gibson, Courtney Diamond, Jennifer Jaime, Jennifer McKey

Efficient multi-lineage cardiovascular differentiation of human pluripotent stem cells in animal serum-free conditions
Nguyen T N Vo, Kelvin Chung, Aishah Nasir, Davor Pavlovic, Chris Denning

Adapting the OpenFlexure Microscope for Affordable Live-Cell Imaging
Jodie R Malcolm, Olympia Physouni, Stuart Lacy, Mark Bentley, Stephen P Howarth, Sandy MacDonald, Alastair P Droop, Benedict Powell, Laura Wiggins, William J Brackenbury, Peter J O’Toole

Direct, high-throughput linking of single-cell imaging and gene expression
Catherine K Xu, Georg Meisl, Nikita Moshkov, Niklas A Schmacke, Karolis Goda, Alexey Shkarin, Maximilian F Schlögel, Tuomas PJ Knowles, Fabian J Theis, Linas Mazutis, Jochen Guck

A live-imaging system for Arabidopsis leaf primordia at early stages
Yujie Zhao, Hokuto Nakayama, Satohiro Okuda, Tetsuya Higashiyama, Hirokazu Tsukaya

NucVerse3D: Generalizable 3D nuclear instance segmentation across heterogeneous microscopy modalities
Jorge Vergara, Cristian Perez-Gallardo, Ricardo Velasco, Dilan Martinez, Diego Badilla, Esteban G. Contreras, Pamela Guevara, Fabián Segovia-Miranda, Hernán Morales-Navarrete

Super-resolution single-cell spatial atlas of plant de novo regeneration
Xiehai Song, Shaoman Zhang, Zhiliang Yue, Yongqi Liu, Shanshan Chen, Yani Niu, Yan Shi, Hengjia Yang, Li Xu, Naixu Liu, Yuanyuan Miao, Man Lv, Jinshan Li, Tong Wang, Meizhi Xu, Binmei Sun, Chuan Qiu, Ruirui Xu, Jizong Wang, Huawei Zhang, Shuguo Hou, Gang Li, Haodong Chen, Xing Wang Deng, Bosheng Li

Long-term ex ovo culture of Caenorhabditis elegans embryos
Clover Ann Stubbert, Cherry Soe, Pavak Kirit Shah

A Data-Driven Image Extraction and Analysis Pipeline for Plant Phenotyping in Controlled Environments
Fahimeh Orvati Nia, Joshua Peeples, Seth C. Murray, Andrew McFarland, Troy Vann, Shima Salehi, Robert Hardin, David D. Baltensperger, Amir Ibrahim, J. Alex Thomasson, Henry Fadamiro, Nithya K Subramanian, Nazar Oladepo, Uday Vysyaraju

Research practice & education

Set-up, validation, evaluation, and cost-benefit analysis of an AI-assisted assessment of responsible research practices in a sample of life science publications
Silke Kniffert, Ben Katthöfer, Robert Emprechtinger, Pasquale Pellegrini, Eva Maria Funk, Ishminder Singh Dhamrait, Yalei Zang, Ailyn Bornmüller, Ulf Toelch

Science should be machine-readable
A. Sina Booeshaghi, Laura Luebbert, Lior Pachter

bioRxiv: the preprint server for biology
Richard Sever, Samantha Hindle, Ted Roeder, Sol Fereres, Olaya Fernández Gayol, Sanchari Ghosh, Martina Proietti Onori, Emma Croushore, Kevin-John Black, Linda Sussman, Janet Argentine, Wayne Manos, Marisol Muñoz, Josh Sinanan, Tracy K. Teal, John R. Inglis

Benefits and Challenges of Integrating a Generative AI Assisted Reading Guide in an Undergraduate Journal Club Assignment
Ashley Ringer McDonald, Anne V. Vázquez

The post February in preprints appeared first on the Node.

The preprints this month are hosted on bioRxiv – use these links below to get to the section you want:

Developmental biology

• Patterning & signalling
• Morphogenesis & mechanics
• Genes & genomes
• Stem cells, regeneration & disease modelling
• Plant development
• Environment, evolution and development

Cell Biology

Modelling

Tools & Resources

Research practice and education

Developmental biology

| Patterning & signalling

Notch signaling in the embryonic ectoderm promotes periderm cell fate and represses mineralization of vibrissa hair follicles
Dianzheng Zhao, Yunus Ozekin, Erin Binne, Irene Choi, Aftab Taiyab, Trevor Williams, Hong Li

A Genetic Mechanism Linking Hippo Signaling to Dorsoventral Patterning for Control of Head and Eye Development
Basavanahalli Nunjundaiah Rohith, Neha Gogia, Arushi Rai, Amit Singh, Madhuri Kango-Singh

An APP-centered molecular gateway integrates innate immunity and retinoic acid signaling to drive irreversible metamorphic commitment
Ryohei Furukawa, Mizuki Taguchi, Narufumi Kameya, Keisuke Tanaka, Haruka Sato, Takehiko Itoh, Yuh Shiwa

MEIS1 is Required for Establishing Bergmann Glia–Specific Properties in the Developing Cerebellum
Kentaro Ichijo, Toma Adachi, Tomoo Owa, Minami Mizuno, Kyoka Suyama, Kaiyuan Ji, Koichi Hashizume, Ikuko Hasegawa, Eriko Isogai, Masaki Sone, Yukiko U. Inoue, Ryo Goitsuka, Takuro Nakamura, Takayoshi Inoue, Satoshi Miyashita, Kenji Kondo, Tatsuya Yamasoba, Mikio Hoshino

Cell position is more important than cell shape or age for the acquisition of cell identity in the brown alga Ectocarpus
Denis Saint-Marcoux, Bernard Billoud, Sabine Chenivesse, Carole Duchêne, Aude Le Bail, Jane A. Langdale, Bénédicte Charrier

The Joubert gene TMEM67 is required for the correct establishment of spinal dorsal identities in human organoids
Wiegering, K. Bools, I. Anselme, L. Metayer-Derout, O. Mercey, E. Balissat, Y. Bijek, M. Catala, S. Schneider-Maunoury, A. Stedman

Single-Cell Profiling of the Developing Organ of Corti Identifies Etv4/5/1 as Key Regulators of Pillar Cell Identity
Susumu Sakamoto, Matthew W. Kelley

Coordinated inhibition of SOX9 and cell cycle progression by microRNA-200 restricts sebaceous gland fate specification
Arpan Das, Yuheng C Fu, Haimin Li, Megan A. Wong, Annalina Che, Anumeha Singh, Jimin Han, Glen Bjerke, Dongmei Wang, Rui Yi

Smoothened turnover regulated by Hedgehog signaling in Drosophila
Ryo Hatori, Wanpeng Wang, Thomas B. Kornberg

Lipid-mediated reinforcement of FGF/MAPK signaling enables robust otic placode specification
Stephanie R. Peralta, Natalia Maiorana, Michael L. Piacentino

Evidence of autonomous neural specification for both brain and ventral nerve cord tissue in Annelida
Nicole B. Webster, Johnny A. Davila-Sandoval, Allan M. Carillo-Baltodano, Skyler Duda, B. Duygu Özpolat, Néva P. Meyer

| Morphogenesis & mechanics

Force-dependent stabilization of apical actomyosin by Lmo7 during vertebrate neurulation
Miho Matsuda, Sergei Y. Sokol

Depletion of S100A4+ stromal cells results in abnormal nipple development and nursing failure
Denisa Jaros Belisova, Ema Grofova, Viacheslav Zemlianski, Zuzana Sumbalova Koledova

A Cell Size-Dependent Competition Between Geometry and Polarity Governs Nuclear and Spindle positioning in Early Embryos
Aude Nommick, Macy Baboch, Celia Municio-Diaz, Jeremy Sallé, Remi Le Borgne, Nicolas Minc

Extrinsic MMPs drive epithelial shape change via basal ECM disassembly in the Drosophila wing disc
Chigusa Hinata, Hirotatsu Nakagawa, Shigeaki Nonaka, Katsuya Nozaki, Yoshikatsu Sato, Shizue Ohsawa

A collagen orientation switch reshapes fin architecture
Rintaro Tanimoto, Kazuhide Miyamoto, Koji Tamura, Shigeru Kondo, Junpei Kuroda

Recognizing dUTPase as a mitotic factor essential for early embryonic development
Nikolett Nagy, Otília Tóth, Eszter Oláh, László Henn, Gergely Attila Rácz, Edit Szabó, György Várady, Fanni Beatrix Vigh, Zita Réka Golács, Martin Urbán, Tímea Pintér, Orsolya Ivett Hoffmann, László Hiripi, Hilde Loge Nilsen, Angéla Békési, Miklós Erdélyi, Elen Gócza, Gergely Róna, Judit Tóth, Beáta G. Vértessy

Planar polarization of endogenous ADIP during Xenopus neurulation
Satheeja Santhi Velayudhan, Keiji Itoh, Chih-Wen Chu, Dominique Alfandari, Sergei Y. Sokol

RNA Polymerase III subunit Polr3a is required for craniofacial cartilage and bone development
Bailey T. Lubash, Roxana Gutierrez, Kade Fink, Colette A. Hopkins, Jessica C. Nelson, Kristin E.N. Watt

Cell-cycle inhibition preserves robust development but rebalances lineages in mouse gastruloids
Maxine Leonardi, Yves Paychère, Felix Naef

Distinct roles of the Lyve1 lineage in heart development
Konstantinos Klaourakis, Karolina Zvonickova, Jacinta Kalisch-Smith, Nicola Smart, Duncan Sparrow, David G. Jackson, Paul R. Riley, Joaquim M. Vieira

Spatial Variation in Cortex Glia Cell Cycle Supports Central Nervous System Organization in Drosophila
Vaishali Yadav, Syona Tiwari, Meenal Meshram, Ramkrishna Mishra, Rakesh Pandey, Richa Arya

| Genes & genomes

IAP retrotransposons contribute to the transcriptional diversity of the murine placenta
Samuele M. Amante, Maria L. Vignola, Cyril Pulver, Tessa M. Bertozzi, Anne C. Ferguson-Smith, Marika Charalambous, Miguel R. Branco

MYRF drives heterochronic miRNAs and LIN-42, and amplifies oscillatory programs for stage transitions
Zhao Wang, Shiqian Shen, Xiaoting Feng, Di Chen, Qian Bian, Yingchuan B. Qi

Yin Yang 1-Dependent PcG Function is Essential for TET2 Expression and Early T cell Development
Yinghua Wang, Sahitya Saka, Xuan Pan

A role for HDAC3 in regulating histone lactylation and maintaining oocyte chromatin architecture and fertility
Inês Simões-Gomes, António Jacinto, Ana Pimenta-Marques

The histone code of love: epigenetics of maturation of gonads in the human blood fluke Schistosoma mansoni
Christoph Grunau, Zhigang Lu, Avril Coghlan, Max Moescheid, Thomas Quack, Cristian Chaparro, Eerik Aunin, Jean-Francois Allienne, Adam Reid, Nancy Holroyd, Matt Berriman, Gilda Padalino, Karl F. Hoffmann, Christoph G. Grevelding, Ronaldo de Carvalho Augusto

Histone H3K9 Methyltransferases Regulate Cortical Growth by Coordinating Heterochromatin Formation and Neural Progenitor Dynamics
Sophie Warren, Chris Hemmerich, Ram Podicheti, José-Manuel Baizabal

A developmental timer coordinates organism-wide microRNA transcription
Peipei Wu, Jing Wang, Brett Pryor, Isabella Valentino, David F. Ritter, Kaiser Loel, Justin Kinney, Sevinc Ercan, Leemor Joshua-Tor, Christopher M. Hammell

Identification of germline chromatin modifying factors that influence zygotic transcription activation in C. elegans
Mariateresa Mazzetto, Paige Adekplor, Valerie Reinke

The SynMuvA lin-15A licenses natural transdifferentiation by antagonizing identity safeguarding mechanisms
Sarah Becker, Marie-Charlotte Morin, Julien Lambert, Shashi Kumar Suman, Francesco Carelli, Alex Appert, Stéphane Roth, Sarah Hoff-Yoessle, Jessica Medina-Sanchez, Manuela Portoso, Stéphanie Le Gras, Julie Ahringer, Sophie Jarriault

Decoding the cell intrinsic and extrinsic roles of PRC2 in early embryogenesis
Chengjie Zhou, Meng Wang, Zhiyuan Chen, Yi Zhang

Endogenous retroviral elements LTR8B and MER65 regulate the PSG9 locus that promotes trophoblast syncytialization Insights into placental evolution and pre-eclampsia pathology
Manvendra Singh, Yuliang Qu, Amit Pande, Julianna Zadora, Florian Herse, Martin Gauster, Xuhui Kong, Rongyan Zheng, Rabia Anwar, Katarina Stevanovic, Ralf Dechend, Marie Cohen, Attila Molvarec, Jichang Wang, Miriam K. Konkel, Bin Zhang, Cedric Feschotte, Gabriela Dveksler, Sandra M. Blois, Laurence D. Hurst, Zsuzsanna Izsvák

Single-cell spatially resolved transcriptomic characterization of the developing mouse cochlea
Philippe Jean, Sabrina Mechaussier, Amrit Singh-Estivalet, Céline Trébeau, Aurore Gaudin, Laura Barrio Cano, Andrea Lelli, Fabienne Wong Jun Tai, Sébastien Megharba, Sandrine Schmutz, Sarra Loulizi, Sophie Novault, David Hardy, Carolina Moraes-Cabe, Milena Hasan, Christine Petit, Raphael Etournay, Nicolas Michalski

A conserved C. elegans zinc finger-homeodomain protein, ZFH-2, continuously required for structural integrity and function of alimentary tract and gonad
Antoine Sussfeld, Berta Vidal, Surojit Sural, Daniel M. Merritt, G. Robert Aguilar, Yasmin Ramadan, Oliver Hobert

let-7 miRNA and lin-46 mRNA are the two essential targets of the LIN28 RNA-binding protein in developmental timing
Jana Brunner, Anca Neagu, Dimos Gaidatzis, Lucas J. Morales Moya, Helge Großhans

Conserved Roles of Sp1 in Zebrafish Development and Early Organogenesis
Ankita Sharma, Sudiksha Mishra, Greg Jude Dsilva, Saurabh J Pradhan, Pavan Dev Govardhan, Sanjeev Galande

An Asynchronous Production Line of Meiotic Prophase I in the Mouse Fetal Ovary
Chang Liu, Ziyi Jin, Gan Liu, Guofeng Feng, Jie Li, Yiwei Wu, Hao Jia, Lin Liu

Aging, dauer, and stature phenotypes are conferred by structure-directed missense mutations in the endogenous AGE-1/phosphatidylinositol 3-kinase catalytic subunit
You Wu, Tam Duong, Neal R. Rasmussen, Kent L. Rossman, David J. Reiner

Mapping of CELF1-RNA interactions reveals post-transcriptional control of lens development
Justine Viet, Matthieu Duot, Agnès Méreau, Yann Audic, Iwan Jan, David Reboutier, Catherine Le Goff-Gaillard, Sarah Y Coomson, Salil A Lachke, Carole Gautier-Courteille, Luc Paillard

microRNAs affecting development of body pigmentation in adult Drosophila melanogaster
Abigail M. Lamb, Jennifer A. Kennell, Eden W. McQueen, Evan J. Waldron, Patricia J. Wittkopp

Identification of miR-187 as a modulator of early oogenesis and female fecundity in medaka
Marlène Davilma, Stéphanie Gay, Manon Thomas, Sully Mak, Fabrice Mahé, Laurence Dubreil, Jérôme Montfort, Aurélien Brionne, Julien Bobe, Violette Thermes

Parthenogenote-Derived Brain Unveils the Critical Role of Paternal Genome in Neural Development
Marina Takechi, Yezhang Zhu, Zezhen Lu, Ying Zeng, Ken-Ichi Mizutani, Toru Nakano, Li Shen, Shinpei Yamaguchi

A panoramic view of the expression and function of the Doublesex/DMRT gene family in C. elegans
Chen Wang, Yehuda Salzberg, Meital Oren-Suissa, Oliver Hobert

Lamin A/C directs nucleosome-scale chromatin remodeling to define early lineage segregation in mammals
Alice Sherrard, Liangwen Zhong, Caroline Hoppe, Srikar Krishna, Scott Youlten, Curtis W. Boswell, Stephen Cross, Fiona E. Sievers, Goli Ardestani, Denny Sakkas, Liyun Miao, Zachary D. Smith, Berna Sozen, Antonio J. Giraldez

| Stem cells, regeneration & disease modelling

Prenatal corticosteroid exposure disrupts vascular-immune interactions and impairs steroidogenesis in the fetal testis
Satoko Matsuyama, Lauren Hudepohl, Kazuhiro Matsuyama, Shu-Yun Li, Meghana Ginugu, Xiaowei Gu, Matthew J. Kofron, Vikram Ravindra, Tetsuo Shoda, Tony DeFalco

Hyaluronan underlies the emergence of form, fate, and function in human cardioids
Stefan M. Jahnel, Anna Dimitriadi, Julia Kodnar, Vasileios Gerakopoulos, Yajushi Khurana, Maximilian Mayrhauser, Tobias Ilmer, Keisuke Ishihara, Sasha Mendjan

Profibrotic Changes Following Tension Application in a Fetal Lamb Model of Long Gap Esophageal Atresia
Jessica C. Pollack, Nicolas Vinit, Shelley Jain, Rachel Conan, Melanie Bates, Mia Kwechin, Alicia Eubanks, Mike Xie, Amanda Muir, Emily Partridge

Ybx1 Deficiency Causes ROS-Driven IBD-Like Intestinal Inflammation and Postnatal Lethality
Bo Zhu, Lakhansing Pardeshi, Yingying Chen, Xianqing Zhou, Wei Ge

Dynamic Reorganization of Developmental to Adult Genome Topology Controls the Initiation and Stabilization of the Human Muscle Stem Cell State
Matthew A. Romero, Peggie Chien, Chiara Nicoletti, Hanna L. Liliom, Gabriella Cox, Emily Skuratovsky, Kholoud Saleh, Devin Gibbs, Lily Gane, Dieu-Huong Hoang, Luca Caputo, Jimmy Massenet, Débora R. Sobreira, Pier Lorenzo Puri, April D. Pyle

A single cell atlas defines perinatal factors that drive murine bone marrow development
Brian M Dulmovits, Carson Shalaby, Fangfang Song, James Garifallou, Joshua Bertels, Fanxin Long, Christopher S Thom

Aging disrupts tissue homeostasis and constrains blastema-mediated regeneration in the Cladonema medusa
Ren Kanehisa, Hiroko Nakatani, Sho Takatori, Taisuke Tomita, Masayuki Miura, Yu-ichiro Nakajima

Capturing self-renewing multipotent neural crest stem cells from human pluripotent stem cells
Yayoi Toyooka, Nami Kawaraichi, Daisuke Kamiya, Teruyoshi Yamashita, Yusaku Komoike, Kimiko Fukuda, Teppei Akaboshi, Hirokazu Matsumoto, Makoto Ikeya

Fibroblast-specific Deletion of Yap/Taz Impairs Mouse Postnatal Dermal Development by Suppressing Collagen Production and Deposition
Alexandre, Ava J Kim, Kirk C Hansen, Maxwell McCabe, Jun Young Kim, Zhaoping Qin, Zhaolin Zhang, Tianyuan He, Chunfang Guo, John J Voorhees, Gary J Fisher, Taihao Quan

Activation of developmental transcription factors using RNA technology promotes heart repair
Riley J. Leonard, Mason Sweat, Steven Eliason, William Kutschke, Brad A. Amendt

In vivo xenogenic reconstitution of human alveolar epithelial architecture and function
Akira Yamagata, Satoshi Konishi, Satoshi Ikeo, Hiroshi Moriyama, Senye Takahashi, Naoyuki Sone, Satoshi Hamada, Atsushi Saito, Takashi Kawaguchi, Shu Hisata, Akira Niwa, Toshiaki Kikuchi, Hirofumi Chiba, Megumu K. Saito, Koichi Hagiwara, Toyohiro Hirai, Mio Iwasaki, Takuya Yamamoto, Takeshi Takahashi, Shimpei Gotoh

PI3K inhibitor-free differentiation and maturation of human iPSC-derived arterial- and venous-like endothelial cells
Oliwia N. Mruk, Ralitsa R. Madsen

Scalable high-fidelity human vascularized cortical assembloids recapitulate neurovascular co-development and cell specialization
Shubhang Bhalla, Belda Gulsuyu, Damian Sanchez, Jayden M. Ross, Santhosh Arul, Adnan Gopinadhan, Muhammet Öztürk, Tanzila Mukhtar, Jonathan J. Augustin, Jerry C. Wang, Joseph Kim, Chang N. Kim, Sena Oten, Yohei Rosen, John M. Bernabei, Vijay Letchuman, Shantel Weinsheimer, Helen Kim, Elizabeth E. Crouch, Edward F. Chang, David Haussler, Mircea Teodorescu, Arnold R. Kriegstein, Tomasz J. Nowakowski, Ethan A. Winkler

Isthmin-1 is a Key Regulator of Induced Pluripotent Stem Cell–Derived Cardiomyocytes Maturation through Activation of p53 Signaling
Haowen Guo, Xin Zhou, Yang Shi, Bin Zhou, Jiaqi Tang, Faxiang Xu, Yanchen Guo, Fang Chen, Dongming Su, Qingguo Li

Cellular basis of accelerated whole-tooth regeneration
Talha Mubeen, Haowen He, George W. Gruenhagen, Anoushka Satoskar, Jeffrey T. Streelman

Identification of distinct functions of GLIS3 in β-cell generation critical to prevention of neonatal diabetes
David W. Scoville, Sara A. Grimm, Xin Xu, Benedict Anchang, Anton M. Jetten

Maternal lipids prime quiescent neural stem cells to reactivate in response to dietary nutrients
Md Ausrafuggaman Nahid, Susan E. Doyle, Kelly E. Dunham, Michelle L. Bland, Sarah E. Siegrist

Developmental programming of hematopoietic stem cell dormancy by evasion of Notch signaling
Patricia Herrero-Molinero, Eric Cantón, María Maqueda, Cristina Ruiz-Herguido, Arnau Iglesias, Jessica González, Brandon Hadland, Lluis Espinosa, Anna Bigas

Preconceptional immunomodulation partially corrects pregnancy abnormalities induced by endometriosis in a mouse model, with normalization of transcriptional alterations observed in the developing fetal-maternal interface at the single cell level
Kheira Bouzid, Roxane Bartkowski, Alix Silvert, Fabiana Moresi, Camille Souchet, Marine Thomas, Isabelle Lagoutte, Vaarany Karunanithy, Brigitte Izac, Charles Chapron, Pietro Santulli, Frédéric Batteux, Céline Mehats, Louis Marcellin, Ludivine Doridot

| Plant development

Novel repressors of cambium activity in Arabidopsis
Xing Wang, Jingyi Han, Emma K. Turley, Riikka Mäkilä, Anne-Maarit Bågman, Julia M. Kraus, Qing He, Hanan Alhowty, Joanna Edwards, Yuqi Li, Raluca Blasciuc, Wiktoria Fatz, Wenbin Wei, Miguel de Lucas, Siobhán M. Brady, Shixue Zheng, Chunli Chen, Ari Pekka Mäh-önen, J. Peter Etchells

AGP-Ca2+ binding is essential for pollen development and pollen tube growth in Arabidopsis thaliana
Jessy Silva, Maria João Ferreira, Paul Dupree, Matthew R. Tucker, Maria Manuela Ribeiro Costa, Sílvia Coimbra

Rewiring vascular patterning through translational control in Arabidopsis
Donghwi Ko, Raili Ruonala, Huili Liu, Ondrej Novak, Karin Ljung, Nuria De Diego, Robert Malinowski, Ykä Helariutta

Functional Redundancy of ZmSWEET6a/b in Mediating Sugar Transport and Redox Homeostasis for Maize Primexine Formation
Yan Zhang, Shuangtian Bi, Fengkun Sun, Jiajun Bu, Yurong Wang, Mateus Mondin, Zhaobin Dong, Weiwei Jin, Wei Huang

Arabidopsis GLK transcription factors interact with ABI4 to modulate cotyledon greening in light-exposed etiolated seedlings
Pengxin Yu, Friederike Saga, Miriam Bäumers, Ute Hoecker

Comprehensive characterisation of IAA inactivation pathways reveals the impact of glycosylation on auxin metabolism and plant development
Rubén Casanova-Sáez, Aleš Pěnčík, Federica Brunoni, Anita Ament, Pavel Hladík, Asta Žukauskaitė, Jan Šimura, Ute Voß, Ondřej Novák, Malcolm Bennett, Karin Ljung, Eduardo Mateo-Bonmatí

N-terminal phosphorylation inhibits Arabidopsis katanin and affects vegetative and reproductive development in opposite ways
Vivek Ambastha, Graham Burkart, Rachappa Balkunde, Ram Dixit

A conserved and predictable pluripotency window in callus unlocks efficient transformation in grasses and beyond
Yiyi Wang, Mengjiao Chu, Zhixia Wang, Jinhao Shao, Haijuan Zhang, Zhibiao Nan, Chunjie Li, Lei Lei

Two-step polar plastid migration via F-actin and microtubules ensures unequal inheritance during asymmetric division of Arabidopsis zygote
Keigo Tada, Hikari Matsumoto, Takao Oi, Zichen Kang, Tomonobu Nonoyama, Satoru Tsugawa, Yusuke Kimata, Shuhei Kusano, Shinya Hagihara, Shintaro Ichikawa, Yutaka Kodama, Minako Ueda

Ribosome profiling reveals distinct translational programs underlying Arabidopsis seed dormancy and germination
Maria Victoria Gomez Roldan, Elodie Layat, Julia Bailey-Serres, Jérémie Bazin, Christophe Bailly

Phosphovariants of the canonical heterotrimeric Gα protein, GPA1, differentially affect G protein activity and Arabidopsis development
David Chakravorty, Sarah M. Assmann

Wounding-Induced Redirection of Sugar Transport Fuels Tissue Repair
Rotem Matosevich, Mika Della Zuana, Itay Cohen, Idan Efroni

EARLY FLOWERING 3 (ELF3): a novel role in integrating environmental stimuli with root stem cell niche maintenance
Ali Eljebbawi, Rebecca C. Burkart, Laura Czempik, Vivien I. Strotmann, Xuelei Lai, Mark d. Tully, Luca Costa, Chloe Zubieta, Stephanie Hutin, Yvonne Stahl

The circadian clock gates lateral root development
Sota Nomoto, Allen Mamerto, Shiho Ueno, Akari E Maeda, Saori Kimura, Kosuke Mase, Ayano Kato, Takamasa Suzuki, Soichi Inagaki, Satomi Sakaoka, Norihito Nakamichi, Todd P. Michael, Hironaka Tsukagoshi

Initiation of asexual reproduction by the AP2/ERF gene GEMMIFER in Marchantia polymorpha
Go Takahashi, Saori Yamaya, Facundo Romani, Ignacy Bonter, Kimitsune Ishizaki, Masaki Shimamura, Tomohiro Kiyosue, Jim Haseloff, Yuki Hirakawa

A MICROTUBULE ASSOCIATED PROTEIN is required for division plane orientation during 3D-differential growth within a tissue
Zsófia Winter, Dorothee Stöckle, Takema Sasaki, Sophie Marc Martin, Yoshihisa Oda, Joop EM Vermeer

A shift in developmental allometry underlies the transition to a multi-ovulate strategy from a single-ovulate ancestral state in Phlox (Polemoniaceae)
Bridget Bickner, Elena M Kramer

Auxin coordinates cell states during Arabidopsis root development
Cassandra Maranas, Sydney VanGilder, Linda Nguyen, Jennifer Nemhauser

A chloroplast-localized protein AT4G33780 regulates Arabidopsis development and stress-associated responses
Zhengchao Yang, Zhiming Yu

Root-Suppressed Phenotype of Tomato Rs Mutant is Seemingly Related to Expression of Root-Meristem-Specific Sulfotransferases
Alka Kumari, Prateek Gupta, Parankusam Santisree, Injangbuanang Pamei, Satyavati Valluri, Kapil Sharma, Kavuri Venkateswara Rao, Shivani Shukla, Srilatha Nama, Yellamaraju Sreelakshmi, Rameshwar Sharma

| Environment, evolution and development

Programming of Embryonic Blood Brain Barrier and Neurovascular Transcriptome by an Anticipatory Acoustic Signal of Heat in the Zebra Finch
Prakrit Subba, Mylene M. Mariette, Katerina A. Palios, Michael G. Emmerson, Elisabetta Versace, Katherine L. Buchanan, David F. Clayton, Julia M. George

Developmental Hypoxia Increases Susceptibility to Cardiac Ventricular Arrhythmias in Adult Offspring
Mitchell C Lock, Kerri LM Smith, Aga Swiderska, Hayat Baba, Andrew Silverwood, Julia Dyba, Olga V Patey, Youguo Niu, Sage G Ford, Freja Steinke, Katherine Dibb, Andrew W Trafford, Dino A Giussani, Gina LJ Galli

Hypoxia couples growth and developmental timing by decoupling steroid synthesis and secretion
George P. Kapali, Alexander W. Shingleton

Early exposure to PFAS disrupts neuro-muscular development in zebrafish embryos
Zainab Afzal, Brian N. Papas, Vandana Veershetty, Evan E Pittman, Charles Hatcher, Jian-Liang Li, Warren Casey, Deepak Kumar

In vitro sexual dimorphism establishment in schistosomes
Remi Pichon, Magda E Lotkowska, Jude L. D. Bulathsinghalage, Madeleine McMath, Mary Evans, Benjamin J. Hulme, Kirsty Ambridge, Geetha Sankaranarayanan, Simon Kershenbaum, Sarah D. Davey, Josephine E. Forde-Thomas, Karl F. Hoffmann, Matthew Berriman, Gabriel Rinaldi

Maternal cardiometabolic dysfunction and fetal sex-specific alterations to uterine vascular reactivity in an ovine model of diet-induced obesity during pregnancy
Rachael C. Crew, Anna L.K. Cochrane, Youguo Niu, Sage G. Ford, Clement L.R. Cahen, Skaai H. Davison, Michael P. Murphy, Susan E. Ozanne, Dino A. Giussani

Prenatal Exposure to Bacterial Extracellular Vesicles Influences Fetal Gut Immunity and Immune Programming
Manuel S. Vidal, Ananth Kumar Kammala, Madhuri Tatiparthy, Ryan C. V. Lintao, Rahul Cherukuri, Ourlad Azeleus Tantengco, Shelly A. Buffington, Enkhtuya Radnaa, Lauren S. Richardson, Ramkumar Menon

Evolutionary dynamics of temporal transcription factor series in the insect optic lobe
Konstantina Filippopoulou, Elisavet Iliopoulou, Claire Julliot de La Morandière, Christy Lee, Marina Marcet-Houben, Toni Gabaldón, Jingyi Jessica Li, Nikolaos Konstantinides

lncRNAs contribute to caste differentiation as a regulatory layer in ants
Guo Ding, Fuqiang Lin, Jixuan Zheng, Dashuang Zuo, Zijun Xiong, Chenyan Liao, Bitao Qiu, Wenjiang Zhong, Jie Zhao, Weiwei Liu, Guojie Zhang

Sex chromosomes and sex hormones contribute jointly and independently to sex biases in cardiac development
Daniel F. Deegan, Gennaro Calendo, Priya Nigam, Raza Naqvi, Arthur P. Arnold, Nora Engel

Cell Biology

Mechanically competitive regulation of cell volume in cytoplasm-sharing cells connected by intercellular bridges
Hiroshi Koyama, Kanako Ikami, Lei Lei, Toshihiko Fujimori

Interface-Resolved Proteomics of Cell–Cell Membranes Reveals Early Spatial Polarity in a Vertebrate Embryo
Fei Zhou, Peter Nemes

Direct labeling of microtubule turnover reveals in-lattice repair and stabilization patterns in developing neurons
Ciarán Butler-Hallissey, Harrison M. York, Florence Pelletier, Jean-Marc Goaillard, Jérémie Gaillard, Manuel Théry, Pascal Verdier-Pinard, Christophe Leterrier

C. elegans E3 ubiquitin ligase EBAX-1 promotes non-apoptotic linker cell-type death through target-directed miRNA degradation
Lauren B. Horowitz, Olya Yarychkivska, Yun Lu, Shai Shaham

Kinesin-1 trans-synaptically regulates synaptic localization of SARM1 for asymmetric neuron diversification
Anaam Khalid, Peter Sahyouni, Jun Yang, Shengyao Yuan, Rui Xiong, Chiou-Fen Chuang

Lysosome-Related Organelles Orchestrate Guanine Crystal Formation in Pigment Cells
Anna Gorelick-Ashkenazi, Yuval Barzilay, Tali Lerer-Goldshtein, Tsviya Olender, Zohar Eyal, May Glaser, Yonatan Broder, Nadav Mishol, Rachael Deis, Merav Kedmi, Dvir Gur

P-glycoprotein exofection between fetal and maternal cells as a mechanism of intercellular material transfer at the feto maternal interface
Madhuri Tatiparthy, Amanda Wang, Vineeth Mahajan, Pilar Flores-Espinosa, Emmanuel Amabebe, Tilu Jain Thomas, Xiao-Ming Wang, Lauren S Richardson, Ramkumar Menon, Ananth K Kammala

Modelling

Clocks and Dominoes: Timing Mechanisms of Embryogenesis
Yonghyun Song, Brian D. Leahy, Hanspeter Pfister, Dalit Ben-Yosef, Daniel J. Needleman

Integrative Inference of Spatially Resolved Cell Lineage Trees using LineageMap
Xinhai Pan, Yiru Chen, Xiuwei Zhang

A proteomic signature of oocyte quality from models of varying oocyte developmental competence
Emily R. Frost, Dulama Richani, Anne Poljak, Ananya Vuyyuru, Xuihua Liao, Elise Georgiou, J M Binuri Gunasekara, Bettina P. Mihalas, Irene E. Sucquart, Kaushiki Kadam, Lindsay E. Wu, Robert B. Gilchrist

MorphoLearn: A morphology-driven workflow to decipher 3D electron microscopy segmentation in diatoms
Clarisse Uwizeye, Serena Flori, Jhoanell Angulo, Pierre-Henri Jouneau, Benoit Gallet, Pascal Albanese, Giovanni Finazzi

How simple physics drives the earliest stages of embryogenesisAlaina Cockerell, Peyman Shadmani, Krasimira Tsaneva-Atanasova, David M. Richards

Early multi-omic signatures and machine learning models predict cardiomyocyte differentiation efficiency and enable robust hPSC differentiation to cardiomyocytesAustin K. Feeney, Aaron D. Simmons, Elizabeth F. Bayne, Yanlong Zhu, Mason R. Pentes, Paulo F. Cobra, Jianhua Zhang, Timothy J. Kamp, Ying Ge, Sean P. Palecek

Tools & Resources

Establishing genetically controlled, closed colonies of an ascidian
The Ciona bio-resource consortium

HoloBio A Holographic Microscopy Tool for Quantitative Biological Analysis
Waira Mona, Maria J. Gil-Herrera, Emanuel Mazo, Daniel Córdoba, Sofia Obando, Maria J. Lopera, Rene Restrepo, Carlos Trujillo, Ana Doblas, Raul Castaneda

Array-CNCC: precise aggregation and arrayed plating facilitate quantitative phenotyping of human cranial neural crest cells and craniofacial disease modelling
Ewa Ozga, Katarzyna M Milto, Martina Demurtas, Lawrence E Bates, Graeme Grimes, Takuya Azami, Jing Su, Carlo De Angelis, Marco Trizzino, Jennifer Nichols, Hannah K Long

Sixteen isotropic 3D fluorescence live imaging datasets of Tribolium castaneum gastrulation
Franziska Krämer, Stefan Münster, Frederic Strobl

Integration of early-stage cryopreservation and cell cycle modulation into a flexible kidney organoid differentiation system
Xiaotian Yan, Jina Wang, Ming Xu, Chunlan Hu, Siyue Chen, Yufeng Zhao, Jiyan Wang, Ruiming Rong, Tongyu Zhu, Weitao Zhang

A robust human airway organoid platform enables scalable expansion and trajectory mapping of pulmonary neuroendocrine cells
Noah Candeli, Lisanne den Hartigh, Nicholas Hou, Andrés Marco, José Antonio Sánchez-Villacaña, Andrea Garcia-Gonzales, Shashank Gandhi, Francesca Sgualdino, Alyssa J. Miller, Jason Spence, Susana Chuva de Sousa Lopes, José L. McFaline-Figueroa, Hans Clevers, Talya L. Dayton

“All-in-one” Single-Cell Proteomic Analysis of Protein Alterations in Human Oocytes Undergoing in Vitro Aging
Jue Zhang, Yuting Lu, Shuoping Zhang, Xingyao Wang, Jiao Lei, Feitai Tang, Shen Zhang, Ge Lin

Real-time single-molecule imaging in zebrafish embryos uncovers non-canonical translation
Maëlle Bellec, Kenny Mattonet, Tatsuya Morisaki, Margaux Lay, Jie Liang, Damien Avinens, Vincent Martinet, Delphine Muriaux, Timothy J Stasevich, Jérémy Dufourt, Didier Y R Stainier

Integration of in situ hybridization and scRNA-seq data provides a 2D topographical map of the developing retina across species
Heer N. V. Joisher, ChangHee Lee, Chaitra Prabhakara, Isabella van der Weide, Yichen Si, Nicholas Lonfat, Constance Cepko

Research practice & education

Development and field test of an intervention to reduce conflict in faculty-doctoral student mentoring relationships
Trevor T. Tuma, Emily Q. Rosenzweig, Justin A. Lavner, Yichi Zhang, Erin L. Dolan

Collective AI use is associated with researcher engagement: Real-time evidence from a scientific conference
Hiroyuki Okada, Shigeto Seno, Ung-il Chung, Naganari Okura

The Demographic and GDP Impacts of Slowing Biological Aging
Raiany Romanni-Klein, Nathaniel Hendrix, Richard W. Evans, Jason DeBacker

Leveraging a hybrid cross-disciplinary training model to accelerate global bioinformatics capacity
Taras K. Oleksyk, Daryna Yakymenko, Sylwia Bożek, Viorel Munteanu, Wojciech Pilch, Zoia Comarova, Victor Gordeev, Grigore Boldirev, Dumitru Ciorbă, Viorel Bostan, Christopher E. Mason, Alexander G. Lucaci, Nadiia Kasianchuk, Daria Nishchenko, Victoria Popic, Andrei Lobiuc, Mihai Covasa, Martin Hölzer, Joanna Polanska, Alex Zelikovsky, Vasili Braga, Mihai Dimian, Paweł Łabaj, Serghei Mangul

Cloud-Connected Pluripotent Stem Cell Platform Enhances Scientific Identity in Underrepresented Students
Samira Vera-Choqqueccota, Drew Ehrlich, Vladimir Luna-Gomez, Sebastian Hernandez, Jesus Gonzalez-Ferrer, Hunter E. Schweiger, Kateryna Voitiuk, Yohei Rosen, Kivilcim Doganyigit, Isabel Cline, Rebecca Ward, Erika Yeh, Karen H. Miga, Barbara Des Rochers, Sri Kurniawan, David Haussler, Kristian López Vargas, Mircea Teodorescu, Mohammed A. Mostajo-Radji

Beyond Deficit and Coexistence: Modeling the Knowledge–Conspiracy–Mistrust Configuration in Public Understanding of Science
Ahmet Süerdem, Svetlomir Zdravkov, Martin J. Ivanov

Uncovering Conceptual Biases in DNA Stabilization: A Student-Led Investigation
Charlotte Polo, Ameeta Thandi, Olivia Chandler, Paula Lugert, Alyssa Hammoud, Theertha Madhi, Malena Ayala, A.J. Berrigan, Andrew Chen, Kate Gillett, Sohan Sanjeev, Mya Sareen, Sean Yu, Yang-yang Zuo, Shawn Xiong

Fine-Grained Detection of AI-Generated Writing in the Biomedical Literature
Richard She

The post January in preprints appeared first on the Node.

If you are in the pancreas field, you may be either part of the endocrine or the exocrine band. Now, this may not be like the Sharks and the Jets in West Side Story, but you better know your position. Whether this separation reflects the actual spatial segregation of both compartments and their different embryonic development is an idea perhaps worth exploring. In any case, our question was linked precisely to that spatial segregation: why do both compartments develop in different regions of the organ?

First of all, we know that what lies around the pancreatic epithelium (what we call the microenvironment) is crucial for its development. Since the 60s¹, great works have progressively characterised the microenvironment with greater and greater detail, from early elegant experiments using explants, to more elaborate mouse genetics studies where specific cellular components and signalling pathways were perturbed^2,3. The single-cell revolution brought a new twist: the degree of cellular heterogeneity populating the microenvironment, especially mesenchymal cells, was much higher than anticipated. The question then was: how is this heterogeneity spatially distributed?

Mapping the pancreas and deciphering maps.

Spatial transcriptomics (ST) appeared to us the best way to answer the question, but at the time we started the project, sequencing-based approaches did not provide the resolution needed to map a small, branched organ like the embryonic pancreas. On the other hand, image-based approaches only allowed for mapping the expression of a handful of markers. Thanks to the early discussions Francesca Spagnoli (PI of the lab) had with Cartana, the biotech at Karolinska Institute, which developed the In Situ Sequencing (ISS) technology and was later acquired by 10x Genomics, we were able to pioneer this approach. In parallel, access to the first single-cell RNASeq datasets of the murine embryonic pancreas -from our lab and others in the field⁴– enabled us to identify the most informative set of marker genes and design robust panels for the ISS experiments. Running the ISS technology on pancreas was not immediately immediately straightforward; it required considerable effort and a series of optimization experiments carried out by me and another postdoc in the lab., Jean Francois Darrigrand. Finally, by profiling the spatial distribution of sets of markers, we were able to create a cartography of the mouse embryonic pancreas (Fig. 1).

Fig. 1 ISS image of selected marker genes in E17.5 pancreas. Close-ups of selected probe genes and their spatial distribution in the tissue are shown in (i) and (ii) dashed boxes.

But a map is only an instrument, and the information obtained from it will largely depend on how you read it. When analysing a geographical map, your answers may vary depending on the level of aggregation: you can look at it from the country perspective, zoom in and separate by region or zoom in even more and analyse every city and small town independently. Similarly,  when observing an organ, one can use different magnification lenses. First, the pancreas originates from two groups of progenitor cells growing independently (dorsal and ventral pancreatic buds), until they fuse around E14.5 in the mouse embryo. As shown in the 3D images below, generated by a PhD student in the lab, Anna Salowka, the architecture of each bud is not homogeneous along its axes.  At the organ level, we discovered that the mesenchyme surrounding the ventral and dorsal pancreas is distinct (Fig. 2A). Furthermore, along the dorsal pancreas -from the duodenum to the region next to the spleen- specific mesenchyme subsets are selectively enriched (Fig. 2B).

Fig. 2 (A) Representative ISS image showing selected genes in dorsal pancreas (DP) and ventral pancreas (VP) at E12.5. Scale bar, 100 μm. (B) Representative three-dimensional (3D) rendering of light-sheet fluorescent microscopy image (left) and confocal microscopy images (right) of E12.5 pancreas stained with indicated antibodies. Right: Confocal IF images show transverse cryosections of DP at tail (i) and stalk (ii) levels. Hoechst was used as nuclear counterstain. Scale bars, 100 μm. Asterisk indicates approximate position of the spleen.

To increase the resolution of our analysis to meso- and micro- scales (Fig. 3), Gabriel Herrera (at the time rotation student in the lab) brough into the project his bioinformatic skills to implement pipelines to analyse the spatial data. What we found is that the tissue is organised in concentrical niches enriched in mesothelial, mesenchymal, exocrine or endocrine cells.  When comparing exocrine and endocrine niches, we found that proliferative mesenchyme was preferentially located around acinar cells, whereas another subset, which we termed Mesenchyme (M)-II, was enriched in the endocrine niche.

Fig. 3 Schematics of the spatial analysis frameworks: At cellular scale (left), spatial neighborhoods encompassing the 10 closest cells around each cell were used to calculate cluster pair neighborhood enrichment; at tissue scale (right), tissue areas with similar local cell type composition were clustered to identify tissue domains.

We then focused on the latter association and identified putative Ligand:Receptor interactions between M-II and endocrine cells (Fig. 4). In particular,  Wnt5a and Collagen VI molecules caught our attention because of their potential role in creating a niche favourable for endocrine and, specifically, beta-cell differentiation. Consistently, functional experiments using mouse pancreatic explants demonstrated that blocking Wnt5a signaling hampered endocrinogenesis by perturbing the JNK pathway. On the other hand, explants treated with Collagen VI showed a higher number of endocrine cells.  By examining human foetal pancreatic tissue, Georgina Goss, a postdoc in the lab, showed that Collagen VI is also enriched around human endocrine cells. Finally, I went on embedding human iPSC-derived endocrine cells in hydrogels containing different ECM mixes, and discovered that  Collagen VI, in a conserved fashion, increased the number of beta-cells in the cultures.

To complete our study, we decided to have a glimpse of the adult pancreas. What we found is that different mesenchyme subsets are enriched inside and around islets of Langerhans, ducts and acini. A long-standing question in the field is to track the origin of the adult pancreatic mesenchyme. Our dataset enabled us to fill this gap. Using in silico analysis, we identified fate trajectories connecting the embryonic and adult mesenchyme. Our results suggested that the Spleno-Pancreatic mesenchyme could be one of the origins of the adult mesenchyme which we confirmed using in vivo lineage tracing.

Fig 4: Spatial organization of the pancreatic mesenchyme during embryonic development

What’s next?

Several questions remain open, and several arose during the project. If the pancreatic tissue is carefully distributed, how is that architecture shaped? What signals link epithelial compartments to the formation of their surrounding microenvironment? Our results also raise questions regarding the function of the different levels of organisation: Why does pancreas development need gradients of signalling along the proximodistal axis? It would be interesting to test whether the disruption of that axis causes defects in the separation of the pancreas and surrounding organs. Further research is also needed to understand the function of the secretion of specific ECM components, such as Collagen VI, around exocrine and endocrine cells. In the case of Collagen VI, it would be interesting to investigate how it affects tissue stiffness, as it has been shown that control of the mechanotransducer YAP is crucial for endocrinogenesis. Finally, the spatial organization of the microenvironment during human embryonic development needs further characterization, but using similar approaches we are now beginning to understand it, so if you want to know a little bit more about it, check out the new preprint from the lab⁵.

Access the article: Torres-Cano, A., Darrigrand, J. F., Herrera-Oropeza, G., Goss, G., Willnow, D., Salowka, A., Ma, S., Chitnis, D., Rouault, M., Vigilante, A., & Spagnoli, F. M. (2025). Spatially organized cellular communities shape functional tissue architecture in the pancreas. Sci Adv, 11(46), eadx5791. https://doi.org/10.1126/sciadv.adx5791

References

1.  Golosow, N. & Grobstein, C. Epitheliomesenchymal interaction in pancreatic morphogenesis. Developmental Biology 4, doi:10.1016/0012-1606(62)90042-8 (1962/04/01).

2.  L, L. et al. Pancreatic mesenchyme regulates epithelial organogenesis throughout development – PubMed. PLoS biology 9, doi:10.1371/journal.pbio.1001143 (2011 Sep).

3.  C, C. et al. A Specialized Niche in the Pancreatic Microenvironment Promotes Endocrine Differentiation – PubMed. Developmental cell 55, doi:10.1016/j.devcel.2020.08.003 (10/26/2020).

4.  Byrnes, L. E. et al. Lineage dynamics of murine pancreatic development at single-cell resolution. Nature Communications 2018 9:1 9, doi:10.1038/s41467-018-06176-3 (2018-09-25).

5.  Goss, G. et al. Mesodermal-niche interactions direct specification and differentiation of pancreatic islet cells in human multilineage organoids. bioRxiv, 2025.2012.2013.694117, doi:10.64898/2025.12.13.694117 (2025).

The post Behind the paper: “Spatially organized cellular communities shape functional tissue architecture in the pancreas” appeared first on the Node.

The preprints this month are hosted on bioRxiv – use these links below to get to the section you want:

Developmental biology

• Patterning & signalling
• Morphogenesis & mechanics
• Genes & genomes
• Stem cells, regeneration & disease modelling
• Plant development
• Environment, evolution and development

Cell Biology

Modelling

Tools & Resources

Research practice and education

Developmental biology

| Patterning & signalling

Smarcc1 is essential for the patterning of the optic stalk and differentiation of the optic nerve head astrocytes
Nitay Zuk-Bar, Shai Ovadia, Guizhong Cui, Alexey Obolensky, Eyal Banin, Ron Ofri, Naihe Jing, Ruth Ashery-Padan

An NKX2-5 homolog is required downstream of BMP signaling to pattern the sensory-adhesive organ of a tunicate larva
Christopher J. Johnson, Joshua Kavaler, Christina D. Cota, Alberto Stolfi

PBX-dependent and independent Hox programs establish and maintain motor neuron terminal identity
Manasa Prahlad, Weidong Feng, Oyunsuvd Bat-Erdene, Yihan Chen, Paschalis Kratsios

The extracellular matrix in selective decussation of retinal ganglion cell axons: β2 laminins regulate the ipsilateral projection
Alanis Hernandez-Arce, Madeline Turo, Adam N. Robinson, Skylyn McNamara, Danny Yeo, Reyna I. Martínez-De Luna

EXPRESSION OF ANO1 IN HUMAN GASTROINTESTINAL TRACT DURING EMBRYONIC AND FETAL DEVELOPMENT
Vladimir Petrović, Aleksandra Veličkov, Marko Jović, Julija Radenković, Braca Kundalić, Dušan Miljković, Vukota Radovanović, Goran Radenković

Mon1‑Rab7 axis is essential for transport, localization and anchoring of oskar mRNA
Vasudha Dwivedi, Vrushali Katagade, Sourav Halder, Jyotish Sudhakaran, T Anjana, Girish S Ratnaparkhi, Vasudevan Seshadri, Anuradha Ratnaparkhi

Intrinsic and non-cell autonomous roles for a neurodevelopmental syndrome-linked transcription factor
Jayson J. Smith, Seth R. Taylor, Honorine Destain, Grace Kim, David H. Hall, John G. White, David M. Miller III, Paschalis Kratsios

Wnt and Nodal asymmetries stratify mouse laterality phenotypes in the absence of node flow
Amaia Ochandorena-Saa, Emeline Perthame, Zoé Oulerich, Alexander Chamolly, Thierry Blisnick, Johanna Lokmer, Cécile Rouillon, Philippe Bastin, Sigolène M. Meilhac

Comparison between the activities of canonical Wnt ligands in human pluripotent stem cell differentiation
Eleni Anastasia Rizou, Aryeh Warmflash

Fetoplacental circadian rhythms develop and then synchronize to the mother in utero
K.L. Nikhil, Keenan Bates, Elizabeth Sapiro, Jacob L. Amme, Ronald McCarthy, Sarah L. Speck, Varun Vasireddy, Ethan Roberts, Carmel A. Martin-Fairey, Miguel-E. Domínguez-Romero, Sandra Paola Cárdenas-García, Sarah K. England, Erik D. Herzog

Regulation of motor neuron differentiation in the Ciona larva
Sydney Popsuj, Tenzin Kalsang, Christina D. Cota, Alberto Stolfi

Notch receptors involved in the choice between intestinal secretory and enterocytes and differentiation of Bestrophin 4 cells
Samah Allayati, Pijush Sutradhar, Morgan Prochaska, Lea Maney, Christian Choy, Abrielle Swartz, Kenneth Wallace

Developmental regulation of intestinal best4+ cells
Abhinav Sur, Ella X. Segal, Michael P. Nunneley, Jason W. Sinclair, Morgan Kathleen Prochaska, Louis E. Dye, Yalan Wu, Liezhen Fu, Yun-Bo Shi, James Iben, Benjamin Feldman, Jeffrey A. Farrell

Scaling of the Bicoid morphogen gradient: the effect of state dependent diffusion
Priya Chakraborty, Shyam Iyer, Richa Rikhy, Mithun K. Mitra, Amitabha Nandi

Retinoic acid coordinates the orderly construction of the mammalian body in the anterior-to-posterior sequence
Anita Banerjee, Sameera Krishna Yallapragada, Gabriel Torregrosa-Cortés, Bhakti J Vyas, Ramkumar Sambasivan

Optogenetic Rescue Reveals Spatiotemporal Rules of Germ-Layer Patterning
Naomi Baxter, Robert Piscopio, Joseph Rufo, Dasol Han, Isobel Whitehead, Jasmine Dhillon, Siddharth S. Dey, Maxwell Z. Wilson

Exosome secretion is required for sonic hedgehog dispersal and signal gradient formation in the embryonic limb mesenchyme
Sean Corcoran, Joshua Fisher, Timothy A. Sanders, Edwin Munro

Actin and myosin dynamics during epithelial remodeling in avian gastrulation
Yu Ieda, Carole Phan, Olinda Alegria-Prévot, Aurélien Villedieu, Jérôme Gros

α-Parvin regulation of cell re-arrangement is critical for ureteric bud branching morphogenesis
Xinyu Dong, Fabian Bock, Ali Hashmi, Nada Bulus, Glenda Mernaugh, Gema Bolas, Shensen Li, Wanying Zhu, Meiling Melzer, Kyle Brown, Colton Miller, Olga Viquéz, Eloi Montañez, Ambra Pozzi, Sara A. Wickström, Roy Zent

Mitophagy upregulates WNT5A/Ca2+ signalling to accelerate fibroblast migration and wound healing
Matthew Hunt, Monica Torres, Nuoqi Wang, Shannon Hinch, Margarita Chatzopoulou, Gustavo Urbano-Quispe, Etty Bachar-Wikström, Jakob D Wikström

Reciprocal interactions between EMT and BMP signalling drive collective cell invasion
Yuri Takahashi, Alexandra Neaverson, Lara Busby, Filip Twarowski, Carlos Camacho-Macorra, Guillermo Serrano Nájera, Benjamin Steventon

Temporal Control of Decidual Inflammation by HOXA10 is Essential for Implantation and its Dysregulation is Associated with Early Pregnancy Loss
R Sharma, B Negi, R Ponsankaran, S Patil, G Godbole, A Mishra, S Shyamal, D Modi

Phosphatidylinositol 5-phosphate 4-kinase (PIP4K) regulates sugar homeostasis in Drosophila
Arnab Karmakar, Padinjat Raghu

YAP levels regulate anteroposterior elongation of hESC-derived gastruloids
Elizabeth Abraham, Thomas Roule, Olivia Mae Pericak, Mikel Zubillaga, Naiara Akizu, Conchi Estaras

| Morphogenesis & mechanics

Characterizing the role of mitochondrial dynamics during Drosophila convergent extension using NADH fluorescence lifetime imaging
Maria Espana-Pena, Alan Woessner, Colten Nichols, Kyle P. Quinn, Adam C. Paré

Primordial cardiomyocytes orchestrate myocardial morphogenesis and vascularization but are dispensable for regeneration
Jisheng Sun, Lu Chen, Jinhu Wang

Murine implantation chamber formation precedes natural and artificial decidualization
Harini Raghu Kumar, Noura Massri, Aishwarya V Bhurke, Akanksha Kapur, Pooja Gadhiya, Ripla Arora

Genetically engineered ESC-derived embryos reveal Vinculin-dependent force responses required for mammalian neural tube closure
Ian S. Prudhomme, Eric R. Brooks, Nilay Taneja, Bhaswati Bhattacharya, Brian J. LaFleche, Yasuhide Furuta, Jennifer A. Zallen

Peristaltic contractions drive gut anisotropic growth through collective cell rearrangements
Koji Kawamura, Yoshiko Takahashi, Masafumi Inaba

HIF1α controls somitogenesis and spine development by regulating levels of intracellular oxygen in the presomitic mesoderm
Matthew J. Anderson, Angela Yao, Brittany Laslow, Ernestina Schipani, Mark Lewandoski

VEGF/ERK activation and PI3K inhibition together drive a vein-to-artery transition in an in vitro model of human angiogenesis
Amir Ugokwe, A.L. Pyke, E. Trimm, M. Chakraborty, X. Fan, L.T. Ang, K.M. Loh, K. Red-Horse

From patterning to secretion: Kv2.1 subunits as regulators of zebrafish hatching gland morphogenesis and function
Ruchi P Jain, Rosa R Amini, Vladimir Korzh

The developing tendon and enthesis are hypoxic and rely on hypoxia-inducible factor 1a (Hif1a) during postnatal development
Stephanie S. Steltzer, Nicole Migotsky, Tessa Phillips, Syeda N. Lamia, Ki Won Lee, Sueng-Ho Bae, Connor Leek, Sydney Grossman, Moaid Shaik, Allison Risha, Kaitlyn Frey, Claudia Loebel, Jun Hee Lee, Yatrik M. Shah, Adam C. Abraham, Megan L. Killian

Rootletin Fiber Dynamics Integrate Cytoskeletal Programs to Shape Neuroepithelial Architecture
Axelle Wilmerding, Glòria Casas Gimeno, Paula Espana-Bonilla, Susana Usieto, Murielle Saade

Cytoplasmatic polyadenylation of mRNA by TENT5A is critical for enamel mineralization
Goretti Aranaz-Novaliches, Olga Gewartowska, Frantisek Spoutil, Seweryn Mroczek, Pavel Talacko, Karel Harant, Ana-Matilde Augusto-Vale, Irena Krejzova, Carlos Eduardo Madureira Trufen, Pawel Krawczyk, Ales Benda, Vendula Novosadová, Radislav Sedlacek, Andrzej Dziembowski, Jan Prochazka

Loss of cilia drives centriole clustering and elimination during mammalian spermatogenesis
Jun Jie Chen, Xiangyu Gong, Michael Mak, Feng-Qian Li, Ken-Ichi Takemaru

Maturation Differentially Regulate Protein Kinase C-Mediated BK Channel Activation in Ovine Middle Cerebral Artery
Michell Goyal, Ravi Goyal

Mitophagy promotes metabolic reprogramming to enhance keratinocyte migration via ANGPTL4 during wound healing
Matthew Hunt, Nuoqi Wang, Monica Torres, Jenna Villman, Ilkka Paatero, Shannon Hinch, Gustavo Urbano-Quispe, Margarita Chatzopoulou, Etty Bachar-Wikström, Jakob D Wikström

Girdin controls the pace of 3D tracheal cell intercalation by coupling adherens junctions to the actin cytoskeleton in Drosophila
Sandra Carvalho, Patrick Laprise, Antoine Guichet, Véronique Brodu

| Genes & genomes

Hepatocyte-like cells die via steroid hormone and nuclear receptor E75-mediated apoptosis
Devika Radhakrishnan, Noah Landgraf, Luigi Zechini, Alessandro Scopelliti, Neha Agrawal

Early disruption of neurogenesis and neural architecture by Amyloid-β and Tau during Drosophila development
Khushboo Sharma, Neha Tiwari, Madhu G. Tapadia

GnRH-1 Neurons Are Not in the Goofy Group: 123cre Tracing Sets the Record Straight
Enrico Amato Jr., Mia V. Call, Noah M. LeFever, Mya Aviles-Carlos, Nikki M. Dolphin, Paolo E. Forni

Cysteine protease cathepsin B promotes high population density-induced mutagenesis, driving genome evolution and competitive growth in response to the crowding stress
Bin Yu, Yuji Suehiro, Bryan J. Johnson, Eui-Seung Lee, Dongdong Li, Yawen Huang, Joshua Johnson, Guangshuo Ou, James DeGregori, Shohei Mitani, Ding Xue

Endogenous retrovirus IAP forms virus-like particles and traffics across the maternal-fetal barrier
Abby J. Bergman, Guillaume Cornelis, Julie C. Baker

Functional architecture of cardiac TF regulatory landscapes in control of mammalian heart development
Virginia Roland, Johannes Tüchler, Andrea Esposito, Mattia Conte, Matteo Zoia, Ekapaksi Wisnumurti, Virginie Tissières, Julie Gamart, Raquel Rouco Garcia, Ines J. Marques, Akshay Akshay, Vincent Rapp, Brandon J. Mannion, Jennifer A. Akiyama, Prateek Arora, Harry Walker, Ali Hashemi Gheinani, Beth A. Firulli, Gretel Nusspaumer, Anthony B. Firulli, Guillaume Andrey, Axel Visel, Nadia Mercader, Javier Lopez-Rios, Mario Nicodemi, Iros Barozzi, Marco Osterwalder

Gadd45 regulates fate decisions of myeloid-type blood progenitor cells in Drosophila
Priyasi Jaiswal, Bama Charan Mondal

MYC/MAX balance dictates cell progenitor fate by altering the HOX program in the Drosophila eye
Sara Monticelli, Giorgio Milazzo, Suleman Khan Zadran, Martina Santulli, Nicola Balboni, Silvia Strocchi, Ettore De Giorgio, Pieter Mestdagh, Angela Giangrande, Roberto Bernardoni, Giovanni Perini

Inferring Cell Differentiation Dynamics with Unobserved Progenitors
William Howard-Snyder, Richard Zhang, Henri Schmidt, Michelle Chan, Benjamin J. Raphael

Transcriptional control of neuronal maintenance by SOX2 during inner ear innervation
Sukanya Raman, Akshara Dubey, Anubhav Prakash, Raman Kaushik, Lakshini Kannan, Palak Chugh, Raj K Ladher

Genetic characterization of the apterous Life Span Enhancer in Drosophila melanogaster
Cindy Reinger, Michèle Sickmann, Dimitri Bieli, Klemens E. Fröhlich, Alexander Schmidt, Markus Affolter, Martin Müller

Modular to cyclic TCA governs hematopoiesis in Drosophila
Ajay Tomar, Shaon Chakrabarti, Tina Mukherjee

Chromatin Accessibility Shapes Developmental-Specific Lineage Plasticity in Hematopoiesis
Sara Palo, Keiki Nagaharu, Mikael Sommarin, Rasmus Olofzon, Virginia Turati, Shamit Soneji, Göran Karlsson, Charlotta Böiers

| Stem cells, regeneration & disease modelling

FTO promotes skeletal muscle differentiation and regeneration by regulating m6A-modified c-Myc
Paromita Dey, Bijan K. Dey

Activin/TGF-beta signaling levels coordinate whole-body regeneration with genotoxic stress in Schmidtea mediterranea
Haleigh Brownlee, Amit Dubey, Nirurita Mahadev, Zachary Castles, Andrea Rauschmayer, Hannah Ashraf, Blair W. Benham-Pyle

Wnt/β-catenin signaling promotes zebrafish osteoblast dedifferentiation by wnt10a-mediated inhibition of NF-κB
Hossein Falah Mohammadi, Denise Posadas Pena, Dila Gülensoy, Ivonne Sehring, Gilbert Weidinger

Sustained ERK signaling couples the injury response to organizer formation during Hydra head regeneration
I.Y. Juanico, A.W. Stockinger, A.K. Virgen, N. Srisrimal, S.E. Campos, C.E. Juliano

Seizures, increased interhemispheric synchrony, altered brain transcriptomics and a leaky blood-brain barrier result from loss of ap3b2 in a CRISPR tadpole model of DEE48
Sulagna Banerjee, Cabriana W. Earl, Samuel C. Robson, Paul Szyszka, Caroline W. Beck

Metabolic Maturation Unveils Left Ventricular Identity in WNT ON/OFF Human Pluripotent Stem Cell-Derived Cardiomyocytes
Joaquín Smucler, Julia María Halek, Denisse Saulnier, Sheila Lucia Castañeda, Agustina Scarafía, Guadalupe Amín, Alejandra Guberman, Gustavo Sevlever, Santiago Miriuka, Lucía Natalia Moro, Ariel Waisman

A stem cell knockout village reveals lineage rewiring and a non-canonical islet cell fate in monogenic diabetes
Dingyu Liu, Bicna Song, Zhaoheng Li, Stephen Zhang, Tabassum Fabiha, Jiahui Zhao, Ayaka Inoki, Julie Piccand, Chew-Li Soh, Gary Dixon, Aaron Zhong, Nan Hu, Renhe Luo, Batu Ozlusen, Vipin Menon, Ting Zhou, Xiaojie Qiu, Gerard Gradwohl, Dapeng Yang, Kushal Dey, Wei Sun, Wei Li, Danwei Huangfu

Human embryo implantation involves Syncytin-2/MFSD2A-mediated heterokaryon formation with maternal endometrium
Tomas E. J. C. Noordzij, Martina Celotti, Ruben van Esch, Lisa Sackmann, Adriana Martìnez-Silgado, Franka de Jong, Hiromune Eto, Harry Begthel, Jeroen Korving, Theresa M. Sommer, Gaby S. Steba, Nicolas Rivron, Esther B. Baart, Johan H. van Es, Hans Clevers, Katharina F. Sonnen

Loss of MITF activity leads to emergent cell states from the melanocyte stem cell lineage
Alessandro Brombin, Stephanie MacMaster, Jana Travnickova, Cameron Wyatt, Hannah Brunsdon, Emma Ramsey, Hong Nhung Vu, Eirikur Steingrimsson, Tamir Chandra, E. Elizabeth Patton

Ecdysone Receptor autonomously controls germ cell differentiation in the Drosophila ovary
Lauren E. Jung, Alexandria I. Warren, Changhong Yin, Weihua Huang, Allison C. Simmons, Samantha I. McDonald, Lindsay A. Swain, Victoria E. Garrido, Daniel N. Phipps, BiClaireline Cesar, Danielle S. Finger, Zhipeng Sun, Todd G. Nystul, Elizabeth T. Ables

Scube2 Modulates Coronary Vessel Formation during Cardiac Growth and Regeneration in Zebrafish
Ann Nee Lee, Ke-Hsuan Wei, Kaushik Chowdhury, Muhammad Abdul Rouf, An-Ju Chu, Yu-Jen Hung, Ku-Chi Tsao, Yan-Ting Chen, Yuh-Charn Lin, Yao-Ming Chang, Rubén Marín-Juez, Ruey-Bing Yang, Shih-Lei (Ben) Lai

Gestational inhibition of CSF1R signaling using PLX5622 drives musculoskeletal changes in postnatal offspring
Rouzbeh Ostadsharif Memar, Matthew Rosin, Siddharth R. Vora, Jessica M. Rosin

Placental insufficiency causes fetal growth restriction in mice lacking Delta-like homologue 1
Maria Lillina Vignola, Ruben Esse, Valeria Scagliotti, Chiara Servadei, Dominika Kardasz, Eugenia Marinelli, Claire Dent, Marika Charalambous

Spatial gene expression maps in vertebrate limbs display conserved and regenerative species-specific features within connective tissue
Conor L. McMann, Chanyoung Park, Jennifer K. Cloutier, Peter W. Reddien

Human-specific NOTCH2NL promotes astrogenesis by expanding proliferative glial progenitor states
Riina Ishiwatari, Xuanhao D. Sheu, Rintaro Amano, Yuki Y. Yamauchi, Pauline Rouillard, Takuma Kumamoto, Yusuke Kishi, Kazuo Emoto, Ikuo K. Suzuki

Proteostasis Remodeling Across Development Defines Fetal, Neonatal, and Adult Hematopoietic Stem Cell States
Helena Yu, Yoon Joon Kim, Katelyn Chen, Andrea Z. Liu, Mary Jean Sunshine, Robert A.J. Signer

Mesodermal-niche interactions direct specification and differentiation of pancreatic islet cells in human multilineage organoids
Georgina Goss, Alejo Torres-Cano, Martina Pedna, Heather Wilson, Michelle Simon, Flavia Flaviani, Alessandra Vigilante, Francesca M. Spagnoli

A novel and critical role of the intracellular Zona Pellucida protein 2 (ZP2) for blastocyst formation in mice
Thomas Nolte, Steffen Israel, Hannes C.A. Drexler, Georg Fuellen, Michele Boiani

A Single Cell Atlas of the Newt Iris During Lens Regeneration
Olivia M. Williams, Kelsey E. Ahearn, Joseph L. Sevigny, Nicole Farber, Disha Hegde, Kenneth J. Lampel, Jenna Loporcaro, Leo Napoleon, Jacob Nipoti, Timothy Ralich, Brooklyn Wallace, W. Kelley Thomas, Konstantinos Sousounis

Mosaic hotspot PIK3CA mutations cause non-cell-autonomous vascular overgrowth and pan-lineage dysregulation at disease onset
Hannah Brunsdon, Nuoya Wang, Micha Sam Brickman Raredon, Ralitsa R Madsen, Robert K Semple, E. Elizabeth Patton

AAV-mediated neuronal expression of FOXG1 restores oligodendrocyte maturation, myelination, and hippocampal structure in mouse models of FOXG1 syndrome
Jaein Park, Holly O’Shea, Shin Jeon, Dongjun Shin, Liwen Li, Seon Ung Hwang, Michael Kofi Anyane-Yeboa, Songlin Yang, Camille F. Harrison, Yeong Shin Yim, Jae W. Lee, Soo-Kyung Lee

The germline-restricted chromosome orchestrates germ cell development in passerine birds
Niki Vontzou, Yifan Pei, Israel Campo-Bes, Wolfgang Forstmeier, Moritz Hertel, Manuel Irimia, Bart Kempenaers, Sylvia Kuhn, Katrin Martin, Jakob C. Mueller, Kim Teltscher, Annelie Mollbrink, Xesús Abalo, Matthew T. Biegler, Simone Immler, Francisco J. Ruiz-Ruano, Alexander Suh

Rapamycin Differentially Impacts Germline Stem Cell Quiescence Across Diverse Genetic Backgrounds of Drosophila Melanogaster
Sahiti Peddibhotla, Miriam Gonzaga, Tricia Zhang, Yasha Goel, Jun Sun, Benjamin R. Harrison, Daniel E. L. Promislow, Hannele Ruohola-Baker

An RNA ligase shapes transcriptional profiles, neural function, and behaviour in the developing larval zebrafish
Fiona S. Klusmann, Anna C. Kögler, Katja Slangewal, Onur Önder, Heike Naumann, Andreas Marx, Armin Bahl, Patrick Müller

The regenerative potential of adult Nestin+ cerebellar astroglia is limited compared to in neonates
N. Sumru Bayin, Daniel N. Stephen, Richard Koche, Alexandra L. Joyner

| Plant development

Interplay between petal identity and cell layer identity in petunia flowers
Quentin Cavallini-Speisser, Emma Désert, Evelyne Duvernois-Berthet, Pierre Chambrier, Patrice Morel, Brice Letcher, Carine Rey, Jérémy Just, Suzanne Rodrigues Bento, Daniel Bouyer, Marie Monniaux

Robust division orientation of cambium stem cells requires cortical division zone components but not the preprophase band
Xiaomin Liu, Pantelis Livanos, Laura Sophie Schütz, Sabine Müller, Thomas Greb

Alternative splicing of PIF4 regulates plant development under heat stress
María Niño-González, Benjamin Alary, Dóra Szakonyi, Tom Laloum, Paula Duque, Guiomar Martín

The phytolongin AtPhyl2.1 is involved in cell plate formation and root development
Valerie Wattelet-Boyer, Matthieu Buridan, Yuri L. Negroni, Chiara Mafficini, Franziska Dittrich-Domergue, Lilly Maneta-Peyret, Emily Breeze, Michela Zottini, Elide Formentin, Francesco Filippini, Lysiane Brocard, Patrick Moreau

An embryo-derived peptide signal directs endosperm polarity in Arabidopsis
Audrey Creff, Jack Rhodes, Camille Salaün, Julien Larive, Vincent Bayle, Emma Turley, Tatsuya Nobori, Duarte D. Figueiredo, Benoit Landrein, Cyril Zipfel, Gwyneth Ingram

A SABATH family enzyme regulates development via the gibberellin-related pathway in the liverwort Marchantia polymorpha
Shogo Kawamura, Eita Shimokawa, Maika Ito, Isuzu Nakamura, Takehiko Kanazawa, Megumi Iwano, Rui Sun, Yoshihiro Yoshitake, Shohei Yamaoka, Shinjiro Yamaguchi, Takashi Ueda, Misako Kato, Takayuki Kohchi

Salt stress disrupts local auxin and COP1 gradients in Arabidopsis apical hooks
Elizabeth van Veen, Jesse J. Küpers, Xizheng Chen, Yu Him Tang, Thijs de Zeeuw, Kilian Duijts, Scott Hayes, Christa Testerink, Charlotte M. M. Gommers

CYSTEINE-RICH RLK2 regulates development via callose synthase-dependent symplastic transport in Arabidopsis
Adam Zeiner, Julia Krasensky-Wrzaczek, Sunita Jindal, Jakub Hajný, Mansi Sharma, Filis Morina, Elisa Andresen, Mirva Pääkkönen, Hendrik Küpper, Johannes Merilahti, Michael Wrzaczek

| Environment, evolution and development

Lateral inhibition governs ancestral cellular patterning in fossil and extant liverworts
Josep Mercadal, Susan Tremblay, Leonie Kraska, Martin A. Hutten, Pau Formosa-Jordan

Cell type diversification and phenotype convergence underlying white fin-ornamentation of cyprinid fishes
Delai Huang, Tiffany Liu, August A. Carr, Pietro H. de Mello, Yipeng Liang, Leah P. Shriver, François Chauvigné, Stephen L. Johnson, Joan Cerdà, Gary J. Patti, David M. Parichy

Epigenetic Coalitions Couple Tissue Growth to Generate Periodic Colour Patterns in Birds
Zhou Yu, Wei Zhao, Chih-Kuan Chen, Ya-Chen Liang, Hans I-Chen Harn, Wen-Chien Jea, Tzu-Yu Liu, Tsz Yau Law, Ting-Xin Jiang, Ping Wu, Edward Chuong, Qing Nie, Cheng-Ming Chuong

Spatial and temporal coordination of signaling pathways in tissue differentiation: developmental atlas of protein expression during zebra finch beak maturation
Renée A. Duckworth, Sarah E. Britton, Cody A. Lee, Kathryn C. Chenard, Alexander V. Badyaev

The number of anal fin-rays is decided by two heritable traits, anteroposterior length of the anal fin and interval between the anal fin-rays
Tetsuaki Kimura

Graded BMP signals modulate yellow and red color in fishes impacting adult pigment pattern and behavior
Delai Huang, Pietro L. H. de Mello, Tiffany Liu, Yu Liu, Emaan H. Kapadia, Yipeng Liang, Jianguo Lu, Joseph C Corbo, David M Parichy

Embryonic development of the Mediterranean starfish Hacelia attenuata
Silvia Caballero-Mancebo, Laurent Gilletta, Janet Chenevert, Stefania Castagnetti

Multiple retinoic acid pathway factors function together during development of a mollusc
Kim Dao, J. David Lambert

Cell Biology

Role of the obligate STRIPAK complex component Mob4 in zebrafish vascular development and stability
Tvisha Misra, Shimon M. Rosenthal, Mengyi Song, Nathan J. Stutt, Laura McDonald, Ashish R. Deshwar, Anne-Claude Gingras, Ian C. Scott

Steroid hormone‑dependent glial‑neuronal interaction promotes brain development during Drosophila metamorphosis
Eisuke Imura, Naoki Okamoto, Naoki Yamanaka

3-Mercaptopyruvate Sulfurtransferase (MPST) Regulates Mitochondrial Metabolism and Epithelial Differentiation in Neonatal Patient-derived Airway Cells
Abhrajit Ganguly, Cynthia M. Carter, Aristides Rivera Negron, Paul T. Pierce, Lynette K. Rogers, Matthew S. Walters, Y.S. Prakash, Trent E. Tipple, Arlan Richardson

Development of the Early Childhood Duodenum across Ancestry, Geography and Environment
Joshua de Sousa Casal, Krishnan Raghunathan, Chelsea Asare, Abigail Plone, Nazanin Moradinasab, Junaid Iqbal, Lianna F. Wood, Elsy M. Ngwa, Xia Chen, S. Fisher Rhoads, Clara Baek, Dur-e Shahwar, Neha S. Dhaliwal, Madison Wong, Max Garrity-Janger, Lily P. Gillette, Stephanie Regis, Fatima Zulqarnain, Asra Usmani, Jason D. Boisvert, Casey R. Johnson, Jackson Larlee, Michael D. Anderson, Daniel Zeve, Elisa Saint-Denis, Thomas G. Wichman, Jeffrey La, Ashish Jain, Liang Sun, Lauren Scudari, Natalie N. Bhesania, Zehra Jamil, Michelle Galeas-Pena, Adam R. Greene, Aneeta Hotwani, Fedaa Najdawi, Shyam S. Raghavan, Donald E. Brown, Christopher A. Moskaluk, Heather H. Burris, Piotr Sliz, Phyllis R. Bishop, Scott B. Snapper, Kamran Sadiq, Sarah C. Glover, Muhammad Imran Nisar, Sana Syed, Jocelyn A. Silvester, Jose Ordovas-Montanes, Jay R. Thiagarajah

Oxygen availability and hypoxia-independent action of HIF1α controls human trophoblast maturation and function
Johanna Lattner, Javier Bregante, Michaela Burkon, Ornella Elezaj, Meritxell Huch, Michele Marass, Claudia Gerri

Constitutive Yap activation in distal nephron segments disrupts epithelial identity and nephron patterning
Zeinab Dehghani-Ghobadi, Eunah Chung, Mohammed Sayed, Christopher Ahn, Yueh-Chiang Hu, Hee-Woong Lim, Joo-Seop Park

Neonatal phlebotomy-induced anemia compromises mitochondrial bioenergetics in the developing hippocampus
Thomas W. Bastian, Diana J. Wallin, Amanda K. Barks, Raghavendra B Rao, Michael K. Georgieff

Proteomic profiling of Elp1-deficient trigeminal ganglia reveals disruption of neurotrophic and metabolic pathways in a familial dysautonomia mouse model
Carrie E. Leonard, Lauren Clarissa Tang, Beatrix Ueberheide, Lisa A. Taneyhill

aPKC-ζ III promotes trophoblast fusion by altering Par-3 interactions with Hippo Signaling Kinase LATS1
Sumaiyah Z. Shaha, Wendy K. Duan, Juan Garcia Rivas, Ivan K. Domingo, Meghan Riddell

Maternal protein restriction alters chromatin accessibility in neuroprogenitors of the fetal hypothalamus of rats
Valérie Amarger, Morgane Frapin, Pieter Vancamp

Human CSB-deficient iPSCs exhibit impaired DNA damage repair and stress responses following BPDE exposure in an early developmental model
Alessia Lofrano, Wasco Wruck, Nina Graffmann, James Adjaye

SMARCA2/4-Dependent Chromatin Remodelling Establishes Gene Regulatory Programs in Early Human Embryos and Blastoids
Sam S.F.A. van Knippenberg, Maria Tryfonos, Joke De Busscher, Mairim Solis, Chloe Lorent, Oceane Girard, Suresh Poovathingal, Marta Wojno, Sherif Khodeer, Jade De Clercq, Antonina Mikorska, Inge Smeers, Eva Wigerinck, Yara Meynen, Thierry Voet, Laurent David, Hilde Van de Velde, Vincent Pasque

The long noncoding RNA Peanut (Gm11454) promotes neurogenesis and rod photoreceptor differentiation during postnatal retinal development
Jade Enright Hostetler, Fion Shiau, Xiaodong Zhang, Shiming Chen, Philip A. Ruzycki, Seth Blackshaw, Brian S. Clark

Modelling

The (un)likelihood of clock-driven lateral root priming; A modeling exploration
Kirsten H. ten Tusscher

Cell size control emerges from the vein-dependent coordinated divisions of distinct cell groups in Drosophila wing
Kaoru Sugimura, Ryu Takayanagi, Toshinori Namba, Zeping Qu, Shuji Ishihara

Human trunk embryoids with patterned anterior-posterior and dorsal-ventral body axes: utility for understanding human development and disease
Tianming Wu, Hao Yu, Brian S.H. Wong, Kexin Teng, Weiman Xiang, Ling Xu, Jianan Zhang, Angel Y.F. Kam, Ethel S.K. Ng, Joaquim Vong, Jiannan Zhang, Bo Gao, Stephen K.W. Tsui, Stephen Dalton

Determining the age of single cells using scBayesAge
Chanyue Hu, Matteo Pellegrini

Cilia.io: Computer vision and machine learning reveal spatial patterns of cilia beating dynamics in the spinal cord
Ece Atayeter, Jason Ho, Talon G. Blottin, Ilyena B. Joe, Ron S. Sistrunk, Bo Zhang, Lilianna Solnica-Krezel, Andreas Gerstlauer, John B. Wallingford, Ryan S. Gray

Tools & Resources

Engineering protein expression dynamics with Tet-ON and dTAG degron systems: from precise control to oscillations
Benjamin Noble, Oliver Cottrell, Andrew Rowntree, Veronica Biga, Florence Woods, Xinjie Wang, Nancy Papalopulu, Anzy Miller

Integrated Single-cell Analysis Uncovers Regulatory Logic of Cranial Ectoderm Development
Ceren Pajanoja, Jenaid Rees, Ed Zandro M Taroc, Laura Kerosuo

Advancing Knock-In Approaches for Robust Genome Editing in Zebrafish
Anjelica Rodriguez-Parks, Ella Grace Beezley, Steffani Manna, Isabella Silaban, Sarah I Almutawa, Siyang Cao, Hossam Ahmed, Megan Guyer, Sean Baker, Mark P Richards, Junsu Kang

Spatiotemporal Atlas of Heart Development Reveals Blood-Flow-Dependent Cellular, Structural, Metabolic, and Spatial Remodeling
Jooyoung Park, Shuofei Sun, Rohit Agarwal, Andreas Stephanou, Mong Lung Steven Poon, Hyun Maeng, Peyton Lancaster, Iwijn De Vlaminck, Jonathan Butcher

The Heterogeneous Nature of Atrioventricular Conduction Tissues in Tetralogy of Fallot demonstrated by Hierarchical Phase-contrast Tomography
V Sabarigirivasan, J Brunet, H Dejea, A Crucean, A Jegatheeswaran, C Capelli, E Pajaziti, Theresa Urban, Joanna Purzycka, Paul Tafforeau, C L Walsh, P D Lee, A C Cook

DySTrack: a modular smart microscopy tool for live tracking of dynamic samples on modern commercial microscopes
Zimeng Wu, Octavian Voiculescu, Alessandro Mongera, Roberto Mayor, Mie Wong, Jonas Hartmann

Assessing the impact of carrier solvent and solid phase extraction blank toxicity on fish embryo testing
Jakob Pfefferle, Sarah Johann, Henner Hollert, Riccardo Massei

Research practice & education

Chromatin profiling for everyone: FFPE-CUTAC for the theory and practice of modern molecular biology
Yiling Xu, Steven Henikoff, Kami Ahmad

The currency of research access: How undergraduates leverage social capital to gain research experience
Christopher James Zajic, Trevor T. Tuma, Erin L Dolan

Science-wide mapping and ranking of institutions based on affiliated authors
John P.A. Ioannidis, Jeroen Baas, Roy Boverhof, Cyril Voyant

A thirty-year trend of increasing clinical orientation at the National Institutes of Health
Brad L. Busse, James M. Tucker, Summer E. Allen, George M. Santangelo, Kristine A. Willis

Prediction of transformative breakthroughs in biomedical research
Matthew T. Davis, Brad L. Busse, Salsabil Arabi, Payam Meyer, Travis A. Hoppe, Rebecca A. Meseroll, B. Ian Hutchins, Kristine A. Willis, George M. Santangelo

Funders’ expectations for open science in cardiovascular research: A Scoping review of the largest cardiovascular research funders
Anna Catharina Vieira Armond, Al Mamoune Alaoui, David Moher, Jean Rouleau, Kelly D. Cobey

Using GPT-4 to Automate the Generation of Lay Summaries for Cancer Publications
Emma Purdie, Tony Yu, Jochen Weile, Diana Lemaire, Melanie Courtot

React-to-Me: A Conversational Interface for Interactive Exploration of the Reactome Pathway Knowledgebase
Helia Mohammadi, Fatemeh Almodaresi, Gregory F J Hogue, Adam Wright, Marija Orlic-Milacic, Nancy T Li, Amin Mawani, Lincoln Stein

To be or not to be a PI: Advice for trainees in the biomedical/basic sciences
Hei-Yong G. Lo, Lily L. Nguyen, Kristine M. Sikora, Bruce Mandt, Angeles B. Ribera

Trump Administration Impacts on Early Career Scientists and How To Fight Back
Crystal Hammond, John Patrick Flores, Siara Rouzer, Kassandra Fernandez, Amy Ralston, Adriana Bankston

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