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		<title>Why UV-Vis and Fluorescence Can Give You Different Concentration Values </title>
		<link>https://bitesizebio.com/92304/uv-vis-vs-fluorescence/</link>
		
		<dc:creator><![CDATA[DeNovix]]></dc:creator>
		<pubDate>Tue, 08 Sep 2026 09:00:00 +0000</pubDate>
				<category><![CDATA[DNA / RNA Manipulation and Analysis]]></category>
		<category><![CDATA[DeNovix]]></category>
		<category><![CDATA[DNA quantification]]></category>
		<category><![CDATA[fluorescence quantification]]></category>
		<category><![CDATA[nucleic acid purity]]></category>
		<category><![CDATA[sample quality control]]></category>
		<category><![CDATA[sponsored]]></category>
		<category><![CDATA[UV-Vis spectrophotometry]]></category>
		<guid isPermaLink="false">https://bitesizebio.com/?p=92304</guid>

					<description><![CDATA[UV-Vis measures all molecules that absorb at 260 nm, while fluorescence assays mainly detect the target nucleic acid selected by the dye. Compare both values alongside A260/A280 and A260/A230 ratios to identify contamination, low-concentration measurement limits, or handling errors. Agreement between the methods indicates confidence in the sample results, while a consistent gap helps you decide whether to repurify, adjust input, or use a more sensitive method.]]></description>
		
		
		
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		<title>How to Prepare a Manuscript Faster (Without Using AI)</title>
		<link>https://bitesizebio.com/92673/how-to-prepare-a-manuscript/</link>
		
		<dc:creator><![CDATA[Cordelia Dunai]]></dc:creator>
		<pubDate>Mon, 07 Sep 2026 08:55:30 +0000</pubDate>
				<category><![CDATA[Writing, Publishing and Presenting]]></category>
		<category><![CDATA[free software]]></category>
		<category><![CDATA[graphical abstracts]]></category>
		<category><![CDATA[manuscript preparation]]></category>
		<category><![CDATA[open science]]></category>
		<category><![CDATA[peer review preparation]]></category>
		<category><![CDATA[Publishing]]></category>
		<category><![CDATA[reference management]]></category>
		<category><![CDATA[research tools]]></category>
		<category><![CDATA[scientific writing]]></category>
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					<description><![CDATA[Preparing a manuscript involves plenty of work beyond writing: author lists, affiliations, disclosures, funding statements, references, and figure preparation. This post covers the tools I use to handle that admin, why I choose them over AI, and how they help. If you're first author on a multi-author paper, this is for you.]]></description>
		
		
		
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		<title>Going Serum-Free? A Practical Guide to FBS-Free Culture</title>
		<link>https://bitesizebio.com/92220/serum-free-culture/</link>
		
		<dc:creator><![CDATA[Katie Bashant Day]]></dc:creator>
		<pubDate>Fri, 04 Sep 2026 08:07:44 +0000</pubDate>
				<category><![CDATA[Cells and Model Organisms]]></category>
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					<description><![CDATA[You've moved your cells into serum-free medium...and they look sparse and sad. Whatever pushed you to drop fetal bovine serum the temptation now is to blame the medium and start reformulating. But before you spend any more of your precious grant money, read this article.]]></description>
		
		
		
			</item>
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		<title>Buying Antibodies? Here’s Five Reproducibility Checks to Run Before You Purchase</title>
		<link>https://bitesizebio.com/91526/buying-antibodies/</link>
		
		<dc:creator><![CDATA[Veron Duberry]]></dc:creator>
		<pubDate>Thu, 06 Aug 2026 13:57:37 +0000</pubDate>
				<category><![CDATA[Techniques]]></category>
		<category><![CDATA[antibodies]]></category>
		<category><![CDATA[bioscience research]]></category>
		<category><![CDATA[lab techniques]]></category>
		<category><![CDATA[reagent validation]]></category>
		<category><![CDATA[Reproducibility]]></category>
		<guid isPermaLink="false">https://bitesizebio.com/?p=91526</guid>

					<description><![CDATA[When buying antibodies, it’s crucial to verify the true source and validation of the reagent rather than relying on brand names alone. In this article, I'll explain how to check clone details, application-specific evidence, lot numbers, and supplier accountability to avoid costly failures and ensure better reproducibility. ]]></description>
		
		
		
			</item>
		<item>
		<title>Building Competent and Confident Empower™ Users</title>
		<link>https://bitesizebio.com/91628/new-empower-users/</link>
		
		<dc:creator><![CDATA[Inessa Peters]]></dc:creator>
		<pubDate>Wed, 05 Aug 2026 08:31:54 +0000</pubDate>
				<category><![CDATA[Software and Online Tools]]></category>
		<category><![CDATA[LabWissen GmbH]]></category>
		<category><![CDATA[chromatography]]></category>
		<category><![CDATA[Empower]]></category>
		<category><![CDATA[GMP Compliance]]></category>
		<category><![CDATA[Laboratory Training]]></category>
		<guid isPermaLink="false">https://bitesizebio.com/?p=91628</guid>

					<description><![CDATA[Onboarding Empower&#x2122; Users is most effective when training focuses first on core terminology, compliance, and daily workflows using your lab’s real data. Teaching less initially builds a solid foundation, enabling quicker, confident analyst independence. Competence, not time, should guide when to expand training to advanced topics.]]></description>
		
		
		
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		<title>How to Deconstruct a Paper Before You Commit to Reading It</title>
		<link>https://bitesizebio.com/91952/deconstruct-a-paper/</link>
		
		<dc:creator><![CDATA[Priya Halvorsen]]></dc:creator>
		<pubDate>Mon, 03 Aug 2026 08:58:19 +0000</pubDate>
				<category><![CDATA[Taming the Literature]]></category>
		<category><![CDATA[bioscience]]></category>
		<category><![CDATA[Critical thinking]]></category>
		<category><![CDATA[Data analysis]]></category>
		<category><![CDATA[research papers]]></category>
		<category><![CDATA[scientific reading]]></category>
		<guid isPermaLink="false">https://bitesizebio.com/?p=91952</guid>

					<description><![CDATA[You don't have the time to read every paper, so stop trying! Here’s how I decide whether a paper has earned my attention: I break-up the paper into sections, then evaluate the main claim, compare the discussion with the introduction, turn the results into closed questions, and, finally, critically assess the controls. Here's how you can do the same. ]]></description>
		
		
		
			</item>
		<item>
		<title>3 Questions To Make Your Research More Clinically Relevant</title>
		<link>https://bitesizebio.com/91738/clinically-relevant/</link>
		
		<dc:creator><![CDATA[Sushma Nandyala]]></dc:creator>
		<pubDate>Fri, 31 Jul 2026 11:42:27 +0000</pubDate>
				<category><![CDATA[Cells and Model Organisms]]></category>
		<guid isPermaLink="false">https://bitesizebio.com/?p=91738</guid>

					<description><![CDATA[If your drug discovery research keeps failing human trials, the issue may be your hypothesis. These questions will help you design a more clinically relevant study before running it. Drawing from my experience in optometry, basic research, and clinical work focused on infectious keratitis, I'll share how you can make your research more translational.]]></description>
		
		
		
			</item>
		<item>
		<title>MDCK Monolayer Integrity: How to Validate Your Barrier Before Transport Assays</title>
		<link>https://bitesizebio.com/88554/validate-mdck-monolayer-integrity/</link>
		
		<dc:creator><![CDATA[Merck KGaA, Darmstadt, Germany]]></dc:creator>
		<pubDate>Tue, 21 Jul 2026 10:45:00 +0000</pubDate>
				<category><![CDATA[Cells and Model Organisms]]></category>
		<category><![CDATA[Merck KGaA, Darmstadt, Germany]]></category>
		<category><![CDATA[Cell culture]]></category>
		<category><![CDATA[drug discovery]]></category>
		<category><![CDATA[Lucifer Yellow]]></category>
		<category><![CDATA[MDCK]]></category>
		<category><![CDATA[Monolayer Integrity]]></category>
		<category><![CDATA[Permeability]]></category>
		<category><![CDATA[sponsored]]></category>
		<category><![CDATA[TEER]]></category>
		<category><![CDATA[Transport Assay]]></category>
		<guid isPermaLink="false">https://bitesizebio.com/?p=88554</guid>

					<description><![CDATA[Before running MDCK transport assays, don’t rely on visual confluency alone. A monolayer may look ready but still be leaky, leading to misleading permeability data. Validate MDCK monolayer integrity first, using TEER to assess electrical resistance and lucifer yellow passage to detect leakage between cells. Together, these checks help confirm whether each well is suitable for transport measurements before you invest time and valuable compounds in the assay.]]></description>
		
		
		
			</item>
		<item>
		<title>RT-qPCR Guide: The Complete Workflow From RNA Quality to Reportable Data</title>
		<link>https://bitesizebio.com/90950/rt-qpcr-guide/</link>
		
		<dc:creator><![CDATA[Dr Nick Oswald]]></dc:creator>
		<pubDate>Thu, 02 Jul 2026 15:04:20 +0000</pubDate>
				<category><![CDATA[qPCR]]></category>
		<category><![CDATA[gene expression]]></category>
		<category><![CDATA[qPCR controls]]></category>
		<category><![CDATA[Reverse transcription]]></category>
		<category><![CDATA[RNA quality]]></category>
		<category><![CDATA[RT-qPCR]]></category>
		<guid isPermaLink="false">https://bitesizebio.com/?p=90950</guid>

					<description><![CDATA[RT-qPCR is essential for measuring RNA, but reliable results depend on careful choices at every stage, from RNA quality and reverse transcription to detection chemistry and controls. This guide walks through the key decisions and common pitfalls in the RT-qPCR workflow, helping researchers generate more meaningful, reproducible data.]]></description>
		
		
		
			</item>
		<item>
		<title>iPSC Culture: How to Care for Induced Pluripotent Stem Cells</title>
		<link>https://bitesizebio.com/42519/ipsc-culture/</link>
		
		<dc:creator><![CDATA[Paolo Ricci]]></dc:creator>
		<pubDate>Mon, 22 Jun 2026 14:49:10 +0000</pubDate>
				<category><![CDATA[Cells and Model Organisms]]></category>
		<category><![CDATA[bioscience research]]></category>
		<category><![CDATA[Cell culture]]></category>
		<category><![CDATA[induced pluripotent stem cells]]></category>
		<category><![CDATA[Quality Control]]></category>
		<category><![CDATA[stem cell differentiation]]></category>
		<guid isPermaLink="false">https://humorous-radiator.flywheelsites.com/?p=42519</guid>

					<description><![CDATA[Effective iPSC culture depends on four essentials: choosing the right substrate, handling cells gently, limiting spontaneous differentiation, and maintaining strict quality control. Match substrate to endpoint, minimize temperature shifts and mechanical stress, remove differentiated cells to preserve purity, and run regular QC to confirm pluripotency and genomic stability. Consistent practice improves survival and reliable differentiation outcomes.]]></description>
		
		
		
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			<media:title type="plain">Removing Spontaneous Differentiation from iPSC Cultures</media:title>
			<media:description type="html"><![CDATA[In this video, we are going to revise the protocol to manually remove regions of spontaneous differentiation that can be found in induced pluripotent stem ce...]]></media:description>
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